LOCUS       RC207219L1                9198 bp    DNA     circular  SYN 
DEFINITION  PDE2A (Myc-DDK-tagged)-Human phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1
ACCESSION   NM_002599
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7489..7669 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8397..9056 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7828..8396 
                     /label="ColE1"  
     misc_feature    6364..6384 
                     /label="LR50 primer"  
     misc_feature    6161..6232 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6373..6964 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6143..6149 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6143 
                     /label="PDE2A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGGCAGGCA TGCGGCCACT CCATCCTCTG 
     3361 CAGGAGCCAG CAGTACCCGG CAGCGCGACC GGCTGAGCCG CGGGGCCAGC AGGTCTTCCT 
     3421 CAAGCCGGAC GAGCCGCCGC CGCCGCCGCA GCAATGCGCC GACAGCCTGC AGGACGCCTT 
     3481 GCTGAGTCTG GGCTCTGTCA TCGACATTTC AGGCCTGCAA CGTGCTGTCA AGGAGGCCCT 
     3541 GTCAGCTGTG CTCCCCCGAG TGGAAACTGT CTACACCTAC CTACTGGATG GTGAGTCCCA 
     3601 GCTGGTGTGT GAGGACCCCC CACATGAGCT GCCCCAGGAG GGGAAAGTCC GGGAGGCTAT 
     3661 CATCTCCCAG AAGCGGCTGG GCTGCAATGG GCTGGGCTTC TCAGACCTGC CAGGGAAGCC 
     3721 CTTGGCCAGG CTGGTGGCTC CACTGGCTCC TGATACCCAA GTGCTGGTCA TGCCGCTAGC 
     3781 GGACAAGGAG GCTGGGGCCG TGGCAGCTGT CATCTTGGTG CACTGTGGCC AGCTGAGTGA 
     3841 TAATGAGGAA TGGAGCCTGC AGGCGGTGGA GAAGCATACC CTGGTCGCCC TGCGGAGGGT 
     3901 GCAGGTCCTG CAGCAGCGCG GGCCCAGGGA GGCTCCCCGA GCCGTCCAGA ACCCCCCGGA 
     3961 GGGGACGGCG GAAGACCAGA AGGGCGGGGC GGCGTACACC GACCGCGACC GCAAGATCCT 
     4021 CCAACTGTGC GGGGAACTCT ACGACCTGGA TGCCTCTTCC CTGCAGCTCA AAGTGCTCCA 
     4081 ATACCTGCAG CAGGAGACCC GGGCATCCCG CTGCTGCCTC CTGCTGGTGT CGGAGGACAA 
     4141 TCTCCAGCTT TCTTGCAAGG TCATCGGAGA CAAAGTGCTC GGGGAAGAGG TCAGCTTTCC 
     4201 CTTGACAGGA TGCCTGGGCC AGGTGGTGGA AGACAAGAAG TCCATCCAGC TGAAGGACCT 
     4261 CACCTCCGAG GATGTACAAC AGCTGCAGAG CATGTTGGGC TGTGAGCTGC AGGCCATGCT 
     4321 CTGTGTCCCT GTCATCAGCC GGGCCACTGA CCAGGTGGTG GCCTTGGCCT GCGCCTTCAA 
     4381 CAAGCTAGAA GGAGACTTGT TCACCGACGA GGACGAGCAT GTGATCCAGC ACTGCTTCCA 
     4441 CTACACCAGC ACCGTGCTCA CCAGCACCCT GGCCTTCCAG AAGGAACAGA AACTCAAGTG 
     4501 TGAGTGCCAG GCTCTTCTCC AAGTGGCAAA GAACCTCTTC ACCCACCTGG ATGACGTCTC 
     4561 TGTCCTGCTC CAGGAGATCA TCACGGAGGC CAGAAACCTC AGCAACGCAG AGATCTGCTC 
     4621 TGTGTTCCTG CTGGATCAGA ATGAGCTGGT GGCCAAGGTG TTCGACGGGG GCGTGGTGGA 
     4681 TGATGAGAGC TATGAGATCC GCATCCCGGC CGATCAGGGC ATCGCGGGAC ACGTGGCGAC 
     4741 CACGGGCCAG ATCCTGAACA TCCCTGACGC ATATGCCCAT CCGCTTTTCT ACCGCGGCGT 
     4801 GGACGACAGC ACCGGCTTCC GCACGCGCAA CATCCTCTGC TTCCCCATCA AGAACGAGAA 
     4861 CCAGGAGGTC ATCGGTGTGG CCGAGCTGGT GAACAAGATC AATGGGCCAT GGTTCAGCAA 
     4921 GTTCGACGAG GACCTGGCGA CGGCCTTCTC CATCTACTGC GGCATCAGCA TCGCCCATTC 
     4981 TCTCCTATAC AAAAAAGTGA ATGAGGCTCA GTATCGCAGC CACCTGGCCA ATGAGATGAT 
     5041 GATGTACCAC ATGAAGGTCT CCGACGATGA GTATACCAAA CTTCTCCATG ATGGGATCCA 
     5101 GCCTGTGGCT GCCATTGACT CCAATTTTGC AAGTTTCACC TATACCCCTC GTTCCCTGCC 
     5161 CGAGGATGAC ACGTCCATGG CCATCCTGAG CATGCTGCAG GACATGAATT TCATCAACAA 
     5221 CTACAAAATT GACTGCCCGA CCCTGGCCCG GTTCTGTTTG ATGGTGAAGA AGGGCTACCG 
     5281 GGATCCCCCC TACCACAACT GGATGCACGC CTTTTCTGTC TCCCACTTCT GCTACCTGCT 
     5341 CTACAAGAAC CTGGAGCTCA CCAACTACCT CGAGGACATC GAGATCTTTG CCTTGTTTAT 
     5401 TTCCTGCATG TGTCATGACC TGGACCACAG AGGCACAAAC AACTCTTTCC AGGTGGCCTC 
     5461 GAAATCTGTG CTGGCTGCGC TCTACAGCTC TGAGGGCTCC GTCATGGAGA GGCACCACTT 
     5521 TGCTCAGGCC ATTGCCATCC TCAACACCCA CGGCTGCAAC ATCTTTGATC ATTTCTCCCG 
     5581 GAAGGACTAT CAGCGCATGC TGGATCTGAT GCGGGACATC ATCTTGGCCA CAGACCTGGC 
     5641 CCACCATCTC CGCATCTTCA AGGACCTCCA GAAGATGGCT GAGGTGGGCT ACGACCGAAA 
     5701 CAACAAGCAG CACCACAGAC TTCTCCTCTG CCTCCTCATG ACCTCCTGTG ACCTCTCTGA 
     5761 CCAGACCAAG GGCTGGAAGA CTACGAGAAA GATCGCGGAG CTGATCTACA AAGAATTCTT 
     5821 CTCCCAGGGA GACCTGGAGA AGGCCATGGG CAACAGGCCG ATGGAGATGA TGGACCGGGA 
     5881 GAAGGCCTAT ATCCCTGAGC TGCAAATCAG CTTCATGGAG CACATTGCAA TGCCCATCTA 
     5941 CAAGCTGTTG CAGGACCTGT TCCCCAAAGC GGCAGAGCTG TATGAGCGCG TGGCCTCCAA 
     6001 CGGTGAGCAC TGGACCAAGG TGTCCCACAA GTTCACCATC CGCGGCATCC CAAGTAACAA 
     6061 CTCGCTGGAC TTCCTGGATG AGGAGTACGA GGTGCCTGAT CTGGATGGCA CTAGGGCCCC 
     6121 CATCAATGGC TGCTGCAGCC TTGATGCTGA GACGCGTACG CGGCCGCTCG AGCAGAAACT 
     6181 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     6241 GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 
     6301 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 
     6361 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 
     6421 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 
     6481 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 
     6541 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 
     6601 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 
     6661 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 
     6721 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 
     6781 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 
     6841 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 
     6901 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 
     6961 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 
     7021 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 
     7081 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 
     7141 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 
     7201 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 
     7261 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 
     7321 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 
     7381 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 
     7441 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 
     7501 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 
     7561 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 
     7621 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 
     7681 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     7741 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     7801 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     7861 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     7921 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     7981 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     8041 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     8101 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     8161 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     8221 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     8281 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     8341 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     8401 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
     8461 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 
     8521 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 
     8581 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 
     8641 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 
     8701 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
     8761 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 
     8821 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 
     8881 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 
     8941 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 
     9001 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 
     9061 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     9121 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG CACATTTCCC 
     9181 CGAAAAGTGC CACCTGAC
//