LOCUS       RC207147                8193 bp    DNA     circular  SYN 
DEFINITION  SMC5 (Myc-DDK-tagged)-Human structural maintenance of chromosomes 5 (SMC5)
ACCESSION   NM_015110
VERSION     NM_015110.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4348..4439 
                     /label="Myc-DDK"  
     misc_feature    4469..5054 
                     /label="PolyA signal"  
     misc_feature    4526..4545 
                     /label="Restriction Enzyme"  
     misc_feature    5330..6002 
                     /label="ColE1"  
     misc_feature    6432..7226 
                     /label="Kan/Neo"  
     misc_feature    7261..7563 
                     /label="SV40 ori"  
     misc_feature    7649..8126 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4323..4330 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4323 
                     /label="SMC5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACTCCG AGCAAGAAGA CGTCAACTCC AAGCCCCCAG CCTTCCAAGA 
     1081 GAGCTCTCCC GAGAGACCCT TCGTCGGAGG TCCCGAGCAA GAGGAAGAAT TCGGCCCCGC 
     1141 AGCTGCCGCT GTTGCAGTCG TCCGGGCCTT TCGTGGAAGG CTCTATCGTC CGCATCTCGA 
     1201 TGGAGAACTT CCTAACATAT GATATTTGTG AAGTATCTCC TGGACCCCAC TTGAATATGA 
     1261 TCGTTGGAGC CAATGGAACA GGGAAGTCGA GCATTGTGTG TGCCATTTGC CTTGGTTTAG 
     1321 CTGGAAAACC TGCTTTCATG GGACGAGCAG ATAAGGTTGG GTTTTTTGTG AAGAGAGGAT 
     1381 GTTCTAGAGG CATGGTTGAA ATTGAATTGT TCAGGGCTTC TGGAAATCTT GTAATCACCC 
     1441 GTGAGATTGA TGTGGCAAAA AATCAGTCCT TTTGGTTCAT CAACAAAAAA TCTACAACCC 
     1501 AGAAAATAGT GGAAGAGAAA GTTGCAGCCT TAAATATTCA AGTGGGGAAT CTTTGCCAGT 
     1561 TTCTCCCTCA GGACAAAGTT GGAGAATTTG CTAAACTCAG CAAAATTGAA CTCCTCGAAG 
     1621 CCACTGAAAA GTCAATTGGT CCCCCAGAAA TGCACAAATA TCACTGTGAA CTCAAAAACT 
     1681 TAAGGGAGAA AGAAAAACAG CTCGAGACCT CATGCAAAGA GAAAACTGAG TATCTACAGA 
     1741 AAATGGTTCA GAGGAATGAA AGATATAAAC AAGATGTGGA GAGGTTCTAT GAACGGAAGC 
     1801 GACATTTAGA TTTAATTGAG ATGCTTGAAG CAAAAAGGCC ATGGGTGGAA TATGAAAATG 
     1861 TTCGTCAGGA ATATGAAGAA GTAAAACTAG TTCGTGACCG AGTGAAGGAA GAGGTCAGAA 
     1921 AACTTAAAGA AGGGCAGATT CCTGTAACAT GTCGAATTGA AGAAATGGAA AACGAGCGTC 
     1981 ACAATTTGGA GGCTCGAATC AAAGAAAAGG CAACAGATAT TAAGGAGGCA TCTCAAAAAT 
     2041 GCAAACAGAA GCAAGATGTT ATAGAAAGGA AAGATAAACA TATTGAGGAA CTTCAGCAGG 
     2101 CTTTAATAGT AAAGCAAAAT GAAGAGCTTG ACCGACAGAG GAGAATAGGT AATACCCGCA 
     2161 AAATGATAGA GGATTTGCAA AATGAACTAA AGACCACGGA AAACTGCGAG AATCTTCAGC 
     2221 CCCAGATTGA TGCCATTACA AATGATCTGA GACGGATTCA GGATGAAAAG GCATTATGTG 
     2281 AAGGCGAAAT AATTGATAAG CGAAGAGAGA GGGAAACTCT AGAGAAGGAG AAAAAGAGTG 
     2341 TGGACGATCA TATTGTACGT TTTGACAATC TTATGAATCA GAAGGAAGAT AAGCTAAGAC 
     2401 AGAGATTCCG TGACACGTAT GATGCTGTTT TATGGCTAAG AAATAACAGA GACAAATTTA 
     2461 AACAAAGAGT CTGTGAGCCC ATAATGCTCA CGATCAATAT GAAAGATAAT AAAAATGCCA 
     2521 AATATATTGA AAATCATATT CCATCAAATG ACTTAAGAGC CTTTGTATTT GAAAGTCAAG 
     2581 AAGATATGGA GGTTTTCCTC AAAGAGGTTC GTGACAATAA AAAATTAAGA GTAAATGCTG 
     2641 TTATTGCTCC CAAGAGTTCA TATGCAGACA AAGCACCTTC AAGATCTTTG AATGAACTTA 
     2701 AACAATACGG ATTTTTCTCT TATTTGAGAG AATTATTTGA TGCACCTGAT CCTGTAATGA 
     2761 GTTACCTTTG CTGTCAGTAT CATATTCATG AAGTTCCTGT AGGAACTGAA AAGACCAGAG 
     2821 AAAGAATTGA ACGGGTAATA CAAGAAACCC GATTAAAACA GATTTATACA GCAGAAGAAA 
     2881 AGTATGTGGT GAAAACTTCT TTTTATTCAA ACAAAGTTAT TTCTAGTAAC ACATCTCTAA 
     2941 AAGTAGCGCA GTTTCTCACT GTCACTGTGG ACCTAGAGCA GAGAAGACAC TTAGAAGAAC 
     3001 AGCTAAAGGA AATTCATAGA AAATTGCAAG CAGTGGATTC AGGGTTGATT GCCTTACGTG 
     3061 AAACAAGCAA ACATCTGGAG CACAAAGACA ATGAACTTAG ACAAAAGAAG AAGGAGCTTC 
     3121 TTGAGAGAAA AACCAAGAAA AGACAACTGG AACAAAAAAT CAGTTCCAAA CTAGGAAGTT 
     3181 TAAAGCTGAT GGAACAGGAT ACTTGCAATC TTGAAGAGGA AGAGCGAAAA GCAAGTACCA 
     3241 AAATCAAAGA AATAAATGTT CAAAAAGCGA AACTTGTTAC CGAATTAACA AACCTAATAA 
     3301 AGATTTGTAC TTCTTTGCAT ATACAAAAAG TAGATTTAAT TCTCCAAAAT ACTACAGTGA 
     3361 TCTCTGAGAA GAACAAATTA GAATCAGATT ATATGGCCGC ATCTTCACAA CTCCGTCTTA 
     3421 CAGAGCAACA TTTCATTGAA TTGGATGAAA ATAGACAGAG ATTATTGCAG AAATGCAAGG 
     3481 AACTTATGAA AAGAGCTAGG CAAGTATGTA ACCTGGGTGC AGAGCAGACT CTTCCTCAAG 
     3541 AATACCAGAC ACAAGTACCC ACCATTCCAA ATGGACACAA CTCCTCACTC CCCATGGTTT 
     3601 TCCAAGACCT TCCAAACACA TTGGATGAAA TTGATGCTTT ATTAACTGAA GAAAGATCAA 
     3661 GAGCTTCCTG CTTCACGGGA CTGAATCCTA CAATTGTTCA GGAATATACA AAAAGAGAAG 
     3721 AAGAAATAGA ACAGTTAACT GAGGAACTAA AGGGAAAGAA AGTTGAACTA GATCAATACA 
     3781 GGGAAAACAT TTCACAGGTA AAAGAAAGGT GGCTTAATCC TTTAAAAGAG CTGGTAGAAA 
     3841 AAATTAATGA AAAATTCAGC AATTTTTTTA GTTCCATGCA GTGTGCTGGT GAAGTTGATC 
     3901 TCCATACAGA AAATGAGGAA GATTATGATA AATATGGAAT TCGAATTAGA GTCAAATTTC 
     3961 GAAGTAGTAC TCAACTGCAT GAATTAACTC CTCATCATCA AAGTGGAGGT GAAAGAAGTG 
     4021 TTTCTACCAT GTTATACTTG ATGGCACTTC AGGAGCTAAA TAGATGTCCA TTCAGAGTAG 
     4081 TTGATGAAAT CAATCAGGGA ATGGACCCAA TCAATGAACG GAGAGTGTTT GAAATGGTTG 
     4141 TAAATACTGC CTGTAAAGAA AATACATCTC AATACTTTTT CATAACACCA AAGCTCCTGC 
     4201 AAAATCTTCC TTATTCTGAA AAGATGACAG TTTTGTTTGT CTACAATGGC CCTCATATGC 
     4261 TGGAACCAAA CACATGGAAT TTAAAGGCTT TCCAAAGGCG GCGGCGCCGT ATTACATTCA 
     4321 CTCAACCTTC TAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4381 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4441 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4501 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4561 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4621 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4681 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4741 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4801 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4861 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4921 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4981 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5041 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5101 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5161 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5221 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5281 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5341 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5401 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5461 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5521 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5581 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5641 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5701 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5761 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5821 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5881 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5941 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6001 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6061 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6121 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6181 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6241 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6301 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6361 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6421 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6481 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6541 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6601 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6661 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6721 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6781 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6841 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6901 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6961 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7021 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7081 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7141 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7201 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7261 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7321 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7381 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7441 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7621 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7681 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7741 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7801 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7861 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7921 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7981 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8041 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8101 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8161 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//