LOCUS       RC207124                8163 bp    DNA     circular  SYN 
DEFINITION  AP3B1 (Myc-DDK-tagged)-Human adaptor-related protein complex 3, beta 1 subunit (AP3B1)
ACCESSION   NM_003664
VERSION     NM_003664.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4320..4411 
                     /label="Myc-DDK"  
     misc_feature    4441..5026 
                     /label="PolyA signal"  
     misc_feature    4498..4517 
                     /label="Restriction Enzyme"  
     misc_feature    5302..5974 
                     /label="ColE1"  
     misc_feature    6404..7198 
                     /label="Kan/Neo"  
     misc_feature    7233..7535 
                     /label="SV40 ori"  
     misc_feature    7621..8098 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4302..4308 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4302 
                     /label="AP3B1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAGCAAT AGTTTTCCTT ACAATGAGCA GTCCGGAGGA GGGGAGGCGA 
     1081 CGGAGCTGGG TCAGGAGGCG ACCTCAACCA TTTCCCCCTC GGGGGCCTTC GGCCTCTTTA 
     1141 GCAGCGATTT GAAGAAGAAT GAAGATCTAA AGCAAATGTT AGAGAGCAAC AAAGATTCTG 
     1201 CTAAACTGGA TGCTATGAAG CGGATTGTTG GGATGATTGC AAAAGGGAAA AATGCATCTG 
     1261 AACTGTTTCC TGCTGTTGTG AAGAATGTGG CCAGTAAAAA TATTGAGATC AAGAAGTTGG 
     1321 TATATGTTTA CCTGGTTCGA TATGCTGAAG AACAGCAGGA TCTTGCACTC CTGTCCATAA 
     1381 GCACTTTTCA GCGAGCTCTG AAGGACCCAA ACCAACTAAT TCGTGCAAGC GCTTTGAGAG 
     1441 TTCTGTCAAG TATTAGAGTG CCAATTATTG TACCTATCAT GATGCTTGCT ATTAAGGAAG 
     1501 CTTCTGCTGA CTTATCACCA TATGTTAGGA AGAATGCAGC CCATGCAATA CAAAAATTAT 
     1561 ACAGCCTTGA TCCAGAGCAG AAGGAAATGT TAATTGAAGT AATTGAAAAA CTTCTGAAAG 
     1621 ATAAAAGCAC ATTGGTAGCT GGCAGTGTTG TGATGGCTTT TGAAGAAGTA TGCCCGGACA 
     1681 GAATAGATCT GATTCATAAA AATTACCGCA AGCTATGTAA CTTACTAGTG GATGTTGAAG 
     1741 AGTGGGGGCA GGTTGTCATA ATCCACATGC TAACTCGATA TGCTCGGACA CAGTTTGTCA 
     1801 GCCCTTGGAA AGAGGGTGAT GAATTAGAAG ACAATGGAAA GAATTTCTAC GAATCTGATG 
     1861 ATGATCAGAA GGAAAAGACT GACAAAAAGA AGAAGCCGTA TACTATGGAT CCAGATCATA 
     1921 GACTCTTAAT TAGAAATACA AAGCCTTTGC TTCAGAGCAG GAATGCTGCG GTGGTTATGG 
     1981 CAGTTGCTCA GCTGTATTGG CACATATCAC CAAAATCTGA AGCTGGCATA ATTTCTAAAT 
     2041 CACTAGTGCG TTTACTTCGT AGCAATAGGG AGGTGCAGTA TATTGTCCTA CAAAATATAG 
     2101 CAACTATGTC AATTCAAAGA AAGGGGATGT TTGAACCTTA TCTGAAGAGT TTCTATGTTA 
     2161 GGTCAACTGA TCCAACTATG ATCAAGACAC TGAAGCTTGA AATTTTGACA AACTTGGCAA 
     2221 ATGAAGCCAA CATATCAACT CTTCTTCGAG AATTTCAGAC CTATGTGAAA AGCCAGGATA 
     2281 AACAATTTGC AGCAGCCACT ATTCAGACTA TAGGCAGATG TGCAACCAAC ATCTTGGAAG 
     2341 TCACTGACAC GTGCCTCAAT GGCTTGGTCT GTCTGCTGTC CAACAGGGAT GAAATAGTTG 
     2401 TTGCTGAAAG TGTGGTTGTT ATAAAGAAAT TACTGCAAAT GCAACCTGCA CAACATGGTG 
     2461 AAATTATTAA ACATATGGCC AAACTCCTGG ACAGTATCAC TGTTCCTGTT GCTAGAGCAA 
     2521 GTATTCTTTG GCTAATTGGA GAAAACTGTG AACGAGTTCC TAAAATTGCC CCTGATGTTT 
     2581 TGAGGAAGAT GGCTAAAAGC TTCACTAGTG AAGATGATCT GGTAAAACTG CAGATATTAA 
     2641 ATCTGGGAGC AAAATTGTAT TTAACCAACT CCAAACAGAC AAAATTGCTT ACCCAGTACA 
     2701 TATTAAATCT CGGCAAGTAT GATCAAAACT ACGACATCAG AGACCGTACA AGATTTATTA 
     2761 GGCAGCTTAT TGTTCCGAAT GAAAAGAGTG GAGCTTTAAG TAAATATGCC AAAAAAATAT 
     2821 TCCTAGCACA AAAGCCTGCA CCACTGCTTG AGTCTCCTTT TAAAGATAGA GATCATTTCC 
     2881 AGCTTGGCAC CTTATCTCAT ACTCTCAACA TTAAAGCTAC TGGGTACCTG GAATTATCTA 
     2941 ATTGGCCAGA GGTGGCGCCC GACCCATCAG TTCGAAATGT AGAAGTAATA GAGTTGGCAA 
     3001 AAGAATGGAC CCCAGCAGGA AAAGCAAAGC AAGAGAATTC TGCTAAGAAG TTTTATTCTG 
     3061 AATCTGAGGA AGAGGAGGAC TCTTCTGATA GTAGCAGTGA CAGTGAGAGT GAATCTGGAA 
     3121 GTGAAAGTGG AGAACAAGGC GAAAGTGGGG AGGAAGGAGA CAGCAATGAG GACAGCAGTG 
     3181 AGGACTCCTC CAGTGAGCAG GACAGTGAGA GTGGACGGGA GTCAGGCCTA GAAAACAAAA 
     3241 GAACAGCCAA GAGGAACTCA AAAGCCAAAG GAAAAAGTGA TTCTGAAGAT GGGGAGAAGG 
     3301 AAAATGAAAA ATCTAAAACT TCAGATTCTT CAAATGACGA ATCTAGTTCA ATAGAAGACA 
     3361 GTTCTTCCGA TTCTGAATCA GAGTCAGAAC CTGAAAGTGA ATCTGAATCC AGAAGAGTCA 
     3421 CTAAGGAGAA AGAAAAGAAA ACAAAGCAAG ATAGAACTCC TCTTACCAAA GATGTTTCAC 
     3481 TTCTAGATCT GGATGATTTT AACCCAGTAT CCACTCCAGT TGCACTTCCC ACACCAGCTC 
     3541 TTTCTCCAAG TTTGATGGCT GATCTTGAAG GTTTACACTT GTCAACTTCC TCTTCAGTCA 
     3601 TCAGTGTCAG TACTCCTGCA TTTGTACCAA CGAAAACTCA CGTGCTGCTT CATCGAATGA 
     3661 GTGGAAAAGG ACTAGCTGCC CATTATTTCT TTCCAAGACA GCCTTGCATT TTTGGTGATA 
     3721 AGATGGTCTC TATACAAATA ACACTGAATA ACACTACTGA TCGAAAGATA GAAAATATCC 
     3781 ACATAGGGGA AAAAAAACTT CCTATAGGCA TGAAAATGCA TGTTTTTAAT CCAATAGACT 
     3841 CTCTTGAGCC TGAGGGATCC ATTACAGTTT CAATGGGTAT TGACTTTTGT GATTCTACTC 
     3901 AGACTGCCAG TTTCCAGTTG TGTACCAAGG ATGATTGCTT CAATGTTAAT ATTCAGCCAC 
     3961 CTGTTGGAGA ACTGCTTTTA CCTGTGGCCA TGTCAGAGAA AGATTTTAAG AAAGAGCAAG 
     4021 GAGTGCTAAC AGGAATGAAT GAAACTTCTG CTGTAATCAT TGCTGCACCA CAGAATTTCA 
     4081 CTCCCTCTGT GATCTTTCAG AAGGTTGTAA ATGTAGCCAA TGTAGGTGCA GTCCCTTCTG 
     4141 GCCAGGATAA TATACACAGG TTTGCAGCTA AAACTGTGCA CAGTGGGTCA TTGATGCTAG 
     4201 TCACAGTGGA ACTGAAGGAA GGCTCTACAG CCCAGCTTAT CATAAACACT GAGAAAACTG 
     4261 TGATTGGCTC TGTTCTGCTG CGGGAACTGA AGCCTGTCCT GTCTCAGGGG ACGCGTACGC 
     4321 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4381 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4441 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4501 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4561 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4621 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4681 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4741 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4801 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4861 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4921 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4981 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5041 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5101 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5161 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5221 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5281 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5341 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5401 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5461 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5521 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5581 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5641 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5701 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5761 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5821 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5881 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5941 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6001 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6061 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6121 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6181 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6241 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6301 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6361 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6421 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6481 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6541 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6601 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6661 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6721 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6781 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6841 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6901 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6961 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7021 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7081 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7141 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7201 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7261 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7321 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7381 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7441 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7501 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7561 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7621 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7681 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7741 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7801 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7861 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7921 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7981 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8041 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8101 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8161 TTT
//