LOCUS       RC207085L1                9417 bp    DNA     circular  SYN 
DEFINITION  PARP1 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase 1 (PARP1)
ACCESSION   NM_001618
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7708..7888 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8616..9275 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8047..8615 
                     /label="ColE1"  
     misc_feature    6583..6603 
                     /label="LR50 primer"  
     misc_feature    6380..6451 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6592..7183 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6362..6368 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6362 
                     /label="PARP1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGAGTCT TCGGATAAGC TCTATCGAGT 
     3361 CGAGTACGCC AAGAGCGGGC GCGCCTCTTG CAAGAAATGC AGCGAGAGCA TCCCCAAGGA 
     3421 CTCGCTCCGG ATGGCCATCA TGGTGCAGTC GCCCATGTTT GATGGAAAAG TCCCACACTG 
     3481 GTACCACTTC TCCTGCTTCT GGAAGGTGGG CCACTCCATC CGGCACCCTG ACGTTGAGGT 
     3541 GGATGGGTTC TCTGAGCTTC GGTGGGATGA TCAGCAGAAA GTCAAGAAGA CAGCGGAAGC 
     3601 TGGAGGAGTG ACAGGCAAAG GCCAGGATGG AATTGGTAGC AAGGCAGAGA AGACTCTGGG 
     3661 TGACTTTGCA GCAGAGTATG CCAAGTCCAA CAGAAGTACG TGCAAGGGGT GTATGGAGAA 
     3721 GATAGAAAAG GGCCAGGTGC GCCTGTCCAA GAAGATGGTG GACCCGGAGA AGCCACAGCT 
     3781 AGGCATGATT GACCGCTGGT ACCATCCAGG CTGCTTTGTC AAGAACAGGG AGGAGCTGGG 
     3841 TTTCCGGCCC GAGTACAGTG CGAGTCAGCT CAAGGGCTTC AGCCTCCTTG CTACAGAGGA 
     3901 TAAAGAAGCC CTGAAGAAGC AGCTCCCAGG AGTCAAGAGT GAAGGAAAGA GAAAAGGCGA 
     3961 TGAGGTGGAT GGAGTGGATG AAGTGGCGAA GAAGAAATCT AAAAAAGAAA AAGACAAGGA 
     4021 TAGTAAGCTT GAAAAAGCCC TAAAGGCTCA GAACGACCTG ATCTGGAACA TCAAGGACGA 
     4081 GCTAAAGAAA GTGTGTTCAA CTAATGACCT GAAGGAGCTA CTCATCTTCA ACAAGCAGCA 
     4141 AGTGCCTTCT GGGGAGTCGG CGATCTTGGA CCGAGTAGCC GATGGCATGG TGTTCGGTGC 
     4201 CCTCCTTCCC TGCGAGGAAT GCTCGGGTCA GCTGGTCTTC AAGAGCGATG CCTATTACTG 
     4261 CACTGGGGAC GTCACTGCCT GGACCAAGTG TATGGTCAAG ACACAGACAC CCAACCGGAA 
     4321 GGAGTGGGTA ACCCCAAAGG AATTCCGAGA AATCTCTTAC CTCAAGAAAT TGAAGGTTAA 
     4381 AAAACAGGAC CGTATATTCC CCCCAGAAAC CAGCGCCTCC GTGGCGGCCA CGCCTCCGCC 
     4441 CTCCACAGCC TCGGCTCCTG CTGCTGTGAA CTCCTCTGCT TCAGCAGATA AGCCATTATC 
     4501 CAACATGAAG ATCCTGACTC TCGGGAAGCT GTCCCGGAAC AAGGATGAAG TGAAGGCCAT 
     4561 GATTGAGAAA CTCGGGGGGA AGTTGACGGG GACGGCCAAC AAGGCTTCCC TGTGCATCAG 
     4621 CACCAAAAAG GAGGTGGAAA AGATGAATAA GAAGATGGAG GAAGTAAAGG AAGCCAACAT 
     4681 CCGAGTTGTG TCTGAGGACT TCCTCCAGGA CGTCTCCGCC TCCACCAAGA GCCTTCAGGA 
     4741 GTTGTTCTTA GCGCACATCT TGTCCCCTTG GGGGGCAGAG GTGAAGGCAG AGCCTGTTGA 
     4801 AGTTGTGGCC CCAAGAGGGA AGTCAGGGGC TGCGCTCTCC AAAAAAAGCA AGGGCCAGGT 
     4861 CAAGGAGGAA GGTATCAACA AATCTGAAAA GAGAATGAAA TTAACTCTTA AAGGAGGAGC 
     4921 AGCTGTGGAT CCTGATTCTG GACTGGAACA CTCTGCGCAT GTCCTGGAGA AAGGTGGGAA 
     4981 GGTCTTCAGT GCCACCCTTG GCCTGGTGGA CATCGTTAAA GGAACCAACT CCTACTACAA 
     5041 GCTGCAGCTT CTGGAGGACG ACAAGGAAAA CAGGTATTGG ATATTCAGGT CCTGGGGCCG 
     5101 TGTGGGTACG GTGATCGGTA GCAACAAACT GGAACAGATG CCGTCCAAGG AGGATGCCAT 
     5161 TGAGCACTTC ATGAAATTAT ATGAAGAAAA AACCGGGAAC GCTTGGCACT CCAAAAATTT 
     5221 CACGAAGTAT CCCAAAAAGT TCTACCCCCT GGAGATTGAC TATGGCCAGG ATGAAGAGGC 
     5281 AGTGAAGAAG CTGACAGTAA ATCCTGGCAC CAAGTCCAAG CTCCCCAAGC CAGTTCAGGA 
     5341 CCTCATCAAG ATGATCTTTG ATGTGGAAAG TATGAAGAAA GCCATGGTGG AGTATGAGAT 
     5401 CGACCTTCAG AAGATGCCCT TGGGGAAGCT GAGCAAAAGG CAGATCCAGG CCGCATACTC 
     5461 CATCCTCAGT GAGGTCCAGC AGGCGGTGTC TCAGGGCAGC AGCGACTCTC AGATCCTGGA 
     5521 TCTCTCAAAT CGCTTTTACA CCCTGATCCC CCACGACTTT GGGATGAAGA AGCCTCCGCT 
     5581 CCTGAACAAT GCAGACAGTG TGCAGGCCAA GGCGGAAATG CTTGACAACC TGCTGGACAT 
     5641 CGAGGTGGCC TACAGTCTGC TCAGGGGAGG GTCTGATGAT AGCAGCAAGG ATCCCATCGA 
     5701 TGTCAACTAT GAGAAGCTCA AAACTGACAT TAAGGTGGTT GACAGAGATT CTGAAGAAGC 
     5761 CGAGATCATC AGGAAGTATG TTAAGAACAC TCATGCAACC ACACACAATG CGTATGACTT 
     5821 GGAAGTCATC GATATCTTTA AGATAGAGCG TGAAGGCGAA TGCCAGCGTT ACAAGCCCTT 
     5881 TAAGCAGCTT CATAACCGAA GATTGCTGTG GCACGGGTCC AGGACCACCA ACTTTGCTGG 
     5941 GATCCTGTCC CAGGGTCTTC GGATAGCCCC GCCTGAAGCG CCCGTGACAG GCTACATGTT 
     6001 TGGTAAAGGG ATCTATTTCG CTGACATGGT CTCCAAGAGT GCCAACTACT GCCATACGTC 
     6061 TCAGGGAGAC CCAATAGGCT TAATCCTGTT GGGAGAAGTT GCCCTTGGAA ACATGTATGA 
     6121 ACTGAAGCAC GCTTCACATA TCAGCAAGTT ACCCAAGGGC AAGCACAGTG TCAAAGGTTT 
     6181 GGGCAAAACT ACCCCTGATC CTTCAGCTAA CATTAGTCTG GATGGTGTAG ACGTTCCTCT 
     6241 TGGGACCGGG ATTTCATCTG GTGTGAATGA CACCTCTCTA CTATATAACG AGTACATTGT 
     6301 CTATGATATT GCTCAGGTAA ATCTGAAGTA TCTGCTGAAA CTGAAATTCA ATTTTAAGAC 
     6361 CTCCCTGTGG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC 
     6421 AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG 
     6481 TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA 
     6541 GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT TCGATATCAA GCTTATCGAT 
     6601 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 
     6661 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 
     6721 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 
     6781 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 
     6841 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 
     6901 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 
     6961 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 
     7021 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 
     7081 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 
     7141 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 
     7201 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 
     7261 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 
     7321 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 
     7381 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 
     7441 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 
     7501 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 
     7561 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 
     7621 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 
     7681 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 
     7741 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 
     7801 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 
     7861 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 
     7921 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     7981 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     8041 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     8101 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     8161 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     8221 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     8281 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     8341 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     8401 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     8461 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     8521 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     8581 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGATTACGC CCCGCCCTGC 
     8641 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 
     8701 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 
     8761 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 
     8821 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA 
     8881 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC 
     8941 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG 
     9001 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC 
     9061 ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA 
     9121 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC 
     9181 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT 
     9241 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATACTCTT CCTTTTTCAA 
     9301 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     9361 TAGAAAAATA AACAAATAGG GGTCCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
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