LOCUS       RC207085                7923 bp    DNA     circular  SYN 
DEFINITION  PARP1 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase 1 (PARP1)
ACCESSION   NM_001618
VERSION     NM_001618.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4080..4171 
                     /label="Myc-DDK"  
     misc_feature    4201..4786 
                     /label="PolyA signal"  
     misc_feature    4258..4277 
                     /label="Restriction Enzyme"  
     misc_feature    5062..5734 
                     /label="ColE1"  
     misc_feature    6164..6958 
                     /label="Kan/Neo"  
     misc_feature    6993..7295 
                     /label="SV40 ori"  
     misc_feature    7381..7858 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4062..4068 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4062 
                     /label="PARP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGTCT TCGGATAAGC TCTATCGAGT CGAGTACGCC AAGAGCGGGC 
     1081 GCGCCTCTTG CAAGAAATGC AGCGAGAGCA TCCCCAAGGA CTCGCTCCGG ATGGCCATCA 
     1141 TGGTGCAGTC GCCCATGTTT GATGGAAAAG TCCCACACTG GTACCACTTC TCCTGCTTCT 
     1201 GGAAGGTGGG CCACTCCATC CGGCACCCTG ACGTTGAGGT GGATGGGTTC TCTGAGCTTC 
     1261 GGTGGGATGA TCAGCAGAAA GTCAAGAAGA CAGCGGAAGC TGGAGGAGTG ACAGGCAAAG 
     1321 GCCAGGATGG AATTGGTAGC AAGGCAGAGA AGACTCTGGG TGACTTTGCA GCAGAGTATG 
     1381 CCAAGTCCAA CAGAAGTACG TGCAAGGGGT GTATGGAGAA GATAGAAAAG GGCCAGGTGC 
     1441 GCCTGTCCAA GAAGATGGTG GACCCGGAGA AGCCACAGCT AGGCATGATT GACCGCTGGT 
     1501 ACCATCCAGG CTGCTTTGTC AAGAACAGGG AGGAGCTGGG TTTCCGGCCC GAGTACAGTG 
     1561 CGAGTCAGCT CAAGGGCTTC AGCCTCCTTG CTACAGAGGA TAAAGAAGCC CTGAAGAAGC 
     1621 AGCTCCCAGG AGTCAAGAGT GAAGGAAAGA GAAAAGGCGA TGAGGTGGAT GGAGTGGATG 
     1681 AAGTGGCGAA GAAGAAATCT AAAAAAGAAA AAGACAAGGA TAGTAAGCTT GAAAAAGCCC 
     1741 TAAAGGCTCA GAACGACCTG ATCTGGAACA TCAAGGACGA GCTAAAGAAA GTGTGTTCAA 
     1801 CTAATGACCT GAAGGAGCTA CTCATCTTCA ACAAGCAGCA AGTGCCTTCT GGGGAGTCGG 
     1861 CGATCTTGGA CCGAGTAGCC GATGGCATGG TGTTCGGTGC CCTCCTTCCC TGCGAGGAAT 
     1921 GCTCGGGTCA GCTGGTCTTC AAGAGCGATG CCTATTACTG CACTGGGGAC GTCACTGCCT 
     1981 GGACCAAGTG TATGGTCAAG ACACAGACAC CCAACCGGAA GGAGTGGGTA ACCCCAAAGG 
     2041 AATTCCGAGA AATCTCTTAC CTCAAGAAAT TGAAGGTTAA AAAACAGGAC CGTATATTCC 
     2101 CCCCAGAAAC CAGCGCCTCC GTGGCGGCCA CGCCTCCGCC CTCCACAGCC TCGGCTCCTG 
     2161 CTGCTGTGAA CTCCTCTGCT TCAGCAGATA AGCCATTATC CAACATGAAG ATCCTGACTC 
     2221 TCGGGAAGCT GTCCCGGAAC AAGGATGAAG TGAAGGCCAT GATTGAGAAA CTCGGGGGGA 
     2281 AGTTGACGGG GACGGCCAAC AAGGCTTCCC TGTGCATCAG CACCAAAAAG GAGGTGGAAA 
     2341 AGATGAATAA GAAGATGGAG GAAGTAAAGG AAGCCAACAT CCGAGTTGTG TCTGAGGACT 
     2401 TCCTCCAGGA CGTCTCCGCC TCCACCAAGA GCCTTCAGGA GTTGTTCTTA GCGCACATCT 
     2461 TGTCCCCTTG GGGGGCAGAG GTGAAGGCAG AGCCTGTTGA AGTTGTGGCC CCAAGAGGGA 
     2521 AGTCAGGGGC TGCGCTCTCC AAAAAAAGCA AGGGCCAGGT CAAGGAGGAA GGTATCAACA 
     2581 AATCTGAAAA GAGAATGAAA TTAACTCTTA AAGGAGGAGC AGCTGTGGAT CCTGATTCTG 
     2641 GACTGGAACA CTCTGCGCAT GTCCTGGAGA AAGGTGGGAA GGTCTTCAGT GCCACCCTTG 
     2701 GCCTGGTGGA CATCGTTAAA GGAACCAACT CCTACTACAA GCTGCAGCTT CTGGAGGACG 
     2761 ACAAGGAAAA CAGGTATTGG ATATTCAGGT CCTGGGGCCG TGTGGGTACG GTGATCGGTA 
     2821 GCAACAAACT GGAACAGATG CCGTCCAAGG AGGATGCCAT TGAGCACTTC ATGAAATTAT 
     2881 ATGAAGAAAA AACCGGGAAC GCTTGGCACT CCAAAAATTT CACGAAGTAT CCCAAAAAGT 
     2941 TCTACCCCCT GGAGATTGAC TATGGCCAGG ATGAAGAGGC AGTGAAGAAG CTGACAGTAA 
     3001 ATCCTGGCAC CAAGTCCAAG CTCCCCAAGC CAGTTCAGGA CCTCATCAAG ATGATCTTTG 
     3061 ATGTGGAAAG TATGAAGAAA GCCATGGTGG AGTATGAGAT CGACCTTCAG AAGATGCCCT 
     3121 TGGGGAAGCT GAGCAAAAGG CAGATCCAGG CCGCATACTC CATCCTCAGT GAGGTCCAGC 
     3181 AGGCGGTGTC TCAGGGCAGC AGCGACTCTC AGATCCTGGA TCTCTCAAAT CGCTTTTACA 
     3241 CCCTGATCCC CCACGACTTT GGGATGAAGA AGCCTCCGCT CCTGAACAAT GCAGACAGTG 
     3301 TGCAGGCCAA GGCGGAAATG CTTGACAACC TGCTGGACAT CGAGGTGGCC TACAGTCTGC 
     3361 TCAGGGGAGG GTCTGATGAT AGCAGCAAGG ATCCCATCGA TGTCAACTAT GAGAAGCTCA 
     3421 AAACTGACAT TAAGGTGGTT GACAGAGATT CTGAAGAAGC CGAGATCATC AGGAAGTATG 
     3481 TTAAGAACAC TCATGCAACC ACACACAATG CGTATGACTT GGAAGTCATC GATATCTTTA 
     3541 AGATAGAGCG TGAAGGCGAA TGCCAGCGTT ACAAGCCCTT TAAGCAGCTT CATAACCGAA 
     3601 GATTGCTGTG GCACGGGTCC AGGACCACCA ACTTTGCTGG GATCCTGTCC CAGGGTCTTC 
     3661 GGATAGCCCC GCCTGAAGCG CCCGTGACAG GCTACATGTT TGGTAAAGGG ATCTATTTCG 
     3721 CTGACATGGT CTCCAAGAGT GCCAACTACT GCCATACGTC TCAGGGAGAC CCAATAGGCT 
     3781 TAATCCTGTT GGGAGAAGTT GCCCTTGGAA ACATGTATGA ACTGAAGCAC GCTTCACATA 
     3841 TCAGCAAGTT ACCCAAGGGC AAGCACAGTG TCAAAGGTTT GGGCAAAACT ACCCCTGATC 
     3901 CTTCAGCTAA CATTAGTCTG GATGGTGTAG ACGTTCCTCT TGGGACCGGG ATTTCATCTG 
     3961 GTGTGAATGA CACCTCTCTA CTATATAACG AGTACATTGT CTATGATATT GCTCAGGTAA 
     4021 ATCTGAAGTA TCTGCTGAAA CTGAAATTCA ATTTTAAGAC CTCCCTGTGG ACGCGTACGC 
     4081 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4141 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4201 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4261 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4321 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4381 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4441 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4501 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4561 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4621 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4681 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4741 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4801 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4861 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4921 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4981 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5041 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5101 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5161 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5221 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5281 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5341 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5401 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5461 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5521 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5581 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5641 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5701 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5761 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5821 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5881 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5941 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6001 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6061 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6121 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6181 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6241 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6301 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6361 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6421 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6481 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6541 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6601 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6661 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6721 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6781 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6841 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6901 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6961 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7021 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7081 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7141 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7201 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7261 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7321 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7381 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7441 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7501 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7561 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7621 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7681 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7741 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7801 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7861 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7921 TTT
//