LOCUS       RC207074                8544 bp    DNA     circular  SYN 
DEFINITION  REXO1 (Myc-DDK-tagged)-Human REX1, RNA exonuclease 1 homolog (S. cerevisiae) (REXO1)
ACCESSION   NM_020695
VERSION     NM_020695.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4701..4792 
                     /label="Myc-DDK"  
     misc_feature    4822..5407 
                     /label="PolyA signal"  
     misc_feature    4879..4898 
                     /label="Restriction Enzyme"  
     misc_feature    5683..6355 
                     /label="ColE1"  
     misc_feature    6785..7579 
                     /label="Kan/Neo"  
     misc_feature    7614..7916 
                     /label="SV40 ori"  
     misc_feature    8002..8479 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4683..4689 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4683 
                     /label="REXO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTACGCTCC ACTGGCTTCT TCCGGGCCAT TGACTGCCCC TATTGGTCTG 
     1081 GGGCGCCCGG GGGGCCCTGC CGGCGGCCCT ACTGCCACTT CCGGCACCGC GGGGCCCGGG 
     1141 GCCCCGGCGC GCCCGGTGAC GGCGGAGAGG CGCCCCCCGC AGCAGGGCTG GGTTACGACC 
     1201 CCTACAACCC TGAGCTGCCC AAGCCCCCCG CGCAGAGGGA GAATGGCACC CTGGGCCTGG 
     1261 GGGAGGAGCC GCGCCCGGAT GTGCTGGAGT TGGAGCTGGT CAACCAGGCC ATCGAGGCCG 
     1321 TGCGCAGTGA GGTGGAGCTG GAGCAGCGGC GCTACCGGGA GCTGCTGGAG ACGACCCGTG 
     1381 AGCACCGCTC CGCCGAGGCC CCCGCCCTGG CGCCCCGCGG CCCCAACGCC AGCCCCACTG 
     1441 TGGGCCCGGA CGAGGATGCC TTCCCACTGG CCTTCGACTA CAGCCCCGGC AGCCACGGCC 
     1501 TATTAAGCCC TGATGCCGGC TACCAGCCCA CCCCACTGGC CGCCCCTGCC GAGCCGGGCA 
     1561 GCAAGTACTC GCTGGCGTCC CTGGACAGGG GTCAGGGCAG AGGTGGAGGG GGTGGCGGTG 
     1621 CCCTGGAATA CGTCCCCAAG GCTGTGAGCC AGCCCCGGCG GCACAGCCGC CCCGTTCCCA 
     1681 GTGGCAAGTA CGTGGTGGAC AACTCCAGGC CACCCACAGA CCTGGAGTAT GACCCTCTCT 
     1741 CCAACTACTC GGCCCGGCAC CTCAGCAGGG CCAGCTCCCG GGATGAGCGG GCCGCCAAGC 
     1801 GGCCCCGGGG CTCCCGCGGC AGTGAGCCCT ACACACCTGC TCCCAAGAAG CTCTGTGACC 
     1861 CCTTTGGCAG TTGCGATGCA AGGTTCTCAG ACTCAGAAGA TGAGGCCGCC ACGGTCCCAG 
     1921 GTAACGAGCC CACCACGGCC AGCACCCCCA AAGCCAGGGC CGACCCTGAG ATCAAGGCCA 
     1981 CCGGGCAGCC ACCCTCCAAA GAGGGCCTGG AGGCCGAGGG GGGCGGCCTG CGGGAGACCA 
     2041 AGGAGACGGC CGTGCAGTGC GACGTGGGGG ACCTCCAGCC GCCCCCAGCC AAGCCCGCCT 
     2101 CCCCAGCCCA GGTCCAGTCC TCACAGGATG GGGGCTGCCC CAAGGAGGGA AAACCCAAGA 
     2161 AGAAAAAAAC CGGGGCCCCA CCTGCCCCCA GCTGCAAAGA CGGGGCCCAG GGGAAGGACA 
     2221 AGACCAAGGA CAAGGGCCGA GGGCGGCCTG TGGAGAAGCC CCGTGCGGAC AAGAAGGGCC 
     2281 CGCAGGCCAG CAGCCCCCGG CGCAAGGCAG AGCGGCCGGA AGGGACCAAG AAGAAGCCAT 
     2341 CTTCGGCCAC TCCTGTGGCC ACCTCAGGGA AAGGGAGGCC TGACCGGCCA GCGCGGCGGC 
     2401 CGAGCCCCAC AAGCGGGGAC TCCCGACCGG CGGCCGGCAG AGGCCCACCC CGCCCCCTCC 
     2461 AGCTGCCCGA CAGGAAGAGC ACCAAGGCCC CGTCGGGGAA GCTAGTGGAG CGGAAAGCCC 
     2521 GCTCACTAGA CGAGGGCGCC TCCCAGGACG CCCCCAAGCT GAAGAAGCGG GCCCTGAGCC 
     2581 ACGCCGACCT CTTTGGGGAC GAGAGTGAGG ACGAGGCCGC AGGGCCAGGG GTGCCGAGCG 
     2641 TGTGGCCCTC TGCCCTCCCC AGCCTCAGCT CGGACTCAGA CTCCGACTCA GACTCCAGCC 
     2701 TGGGCTTCCC GGAGGCGCAG GGGCCGCCCA AGCGGCTCAA GGCCTCCCCG CCCCCCTCCC 
     2761 CCGCCCCATC CTCCTCCTCC TCCTCCTCCT CCTCCACCTC CAGCGCGGGG GCGGATGTGG 
     2821 ACTACTCGGC CCTGGAGAAG GAGGTGGACT TTGACTCCGA CCCCATGGAG GAGTGCCTGC 
     2881 GGATCTTCAA CGAGTCCACC AGCGTCAAGA CGGAGGACAG AGGCCGGCTG GCCCGGCAGC 
     2941 CCCCCAAGGA AGAGAAGAGT GAGGAGAAGG GGCTTTCGGG TCTGACCACT CTGTTCCCCG 
     3001 GGCAGAAGAG GAGGATCTCC CACCTTTCCA AGCAAGGCCA GGAGGTGGAG CCCCCGAGGA 
     3061 GGGGTCCCGC GGTGCCCCCG GCCCGGCCCC CGACGGCGCA GGAGGTGTGC TACCTGCGGG 
     3121 CCCAGCAGGC GCAGAGGGCA TCGGCGAGCT TGCTGCAGGC CCCCGCCAGG CTGGCAGAGA 
     3181 AGTCGCCCTC CGTCCACATT TCCGCCCCTG GCGAGAAGAG GAGGATCGCC CACATCCCCA 
     3241 ACCCCCGCCT GGCTGCAGCC CCCACAGGTG CCAAGAGGAC CCTTGCGGCC AGCGGCAGCC 
     3301 AGCCCTCCAA CGGCCCTGAG CCAGGTGGCC AGCAGCTGAA AACACGCACA TTGTCGGGGA 
     3361 TGGCGTCCAA GACTACCACC ACCATCATCC CTAAGCGAAT CGCCCACAGT CCATCCTTAC 
     3421 AGAGTTTAAA GAAACCCATT ATCCCCAAAG AGTTTGGGGG CAAAGTCCCC ACCGTCATCC 
     3481 GCCAGCGCTA TCTCAACCTG TTCATCGAGG AGTGTCTCAA GTTCTGTACC TCCAACCAGG 
     3541 AGGCCATAGA GAAGGCACTG AACGAGGAGA AGGTGGCCTA TGACCGCAGC CCCAGCAAGA 
     3601 ACATCTACCT GAATGTGGCC GTGAACACCC TCAAGAAGCT CAGGGGCCTG GCCCCCAGCG 
     3661 CTGTGCCCGG CCTCAGCAAA ACCGGTGGCC GCAGGGTTGT GTCCCACGAG GTGGTGTTGG 
     3721 GGGGCAGGTT GGCCGCCAAG ACCAGCTTCT CGCTCAGCCG TCCAAGCAGC CCCCGGGTGG 
     3781 AGGACCTGAA AGGGGCTGCC CTGTACAGCC GCCTCAGGGA GTACCTGCTC ACCCAGGACC 
     3841 AGCTCAAGGA GAACGGCTAC CCCTTCCCGC ACCCAGAGCG GCCCGGGGGC GCAATCATCT 
     3901 TCACAGCTGA GGAGAAGAGG CCCAAGGACT CTTCCTGCAG GACCTGCTGC CGCTGTGGCA 
     3961 CCGAGTACCT CGTGTCCTCT TCAGGCCGCT GCATCCGGGA CGAGGAGTGT TATTACCACT 
     4021 GGGGACGGCT GCGCCGGAAC CGGGTGGCCG GAGGCTGGGA GACCCAGTAC ATGTGCTGCT 
     4081 CGGCTGCCGC CGGCTCTGTC GGCTGCCAAG TCGCAAAGCA ACACGTGCAG GATGGCCGGA 
     4141 AGGAGCGCCT TGAGGGCTTC GTGAAGACCT TTGAGAAAGA GCTCTCAGGA GACACCCACC 
     4201 CGGGGATCTA CGCCCTGGAC TGCGAGATGT CCTACACCAC ATATGGCCTG GAGCTGACGC 
     4261 GCGTCACGGT GGTCGACACG GACGTGCACG TGGTTTATGA CACCTTCGTG AAGCCTGACA 
     4321 ACGAGATCGT GGACTACAAC ACCAGGTTTT CGGGGGTGAC GGAGGCTGAC CTTGCCGACA 
     4381 CAAGTGTCAC GCTGCGTGAC GTCCAGGCCG TTCTGCTGAG CATGTTCAGC GCTGACACCA 
     4441 TCCTCATCGG ACACAGCCTG GAGAGCGACC TCCTGGCCCT GAAGGTCATC CACAGCACCG 
     4501 TGGTGGACAC GTCTGTGCTC TTCCCCCACC GCCTGGGCCT CCCCTACAAG CGGTCCCTGC 
     4561 GGAACCTCAT GGCCGACTAC CTCAGACAGA TCATCCAGGA CAATGTGGAT GGGCACAGCT 
     4621 CCAGCGAGGA CGCCGGCGCC TGCATGCACC TGGTGATCTG GAAGGTTCGA GAAGACGCCA 
     4681 AGACCAAGCG AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4741 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4801 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4861 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4921 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4981 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5041 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5101 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5161 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5221 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5281 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5341 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5401 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5461 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5521 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5581 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5641 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5701 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5761 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5821 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5881 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5941 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6001 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6061 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6121 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6181 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6241 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6301 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6361 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6421 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6481 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6541 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6601 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6661 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6721 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6781 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6841 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6901 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6961 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7021 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7081 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7141 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7201 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7261 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7321 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7381 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7441 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7501 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7561 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7621 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7681 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7741 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7801 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7861 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7981 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8041 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8101 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8161 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8221 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8281 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8341 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8401 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8521 TAACAAAAAT TTAACGCGAA TTTT
//