LOCUS       RC207045                8073 bp    DNA     circular  SYN 
DEFINITION  NLRP12 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2
ACCESSION   NM_144687
VERSION     NM_144687.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4228..4319 
                     /label="Myc-DDK"  
     misc_feature    4349..4934 
                     /label="PolyA signal"  
     misc_feature    4406..4425 
                     /label="Restriction Enzyme"  
     misc_feature    5210..5882 
                     /label="ColE1"  
     misc_feature    6312..7106 
                     /label="Kan/Neo"  
     misc_feature    7141..7443 
                     /label="SV40 ori"  
     misc_feature    7529..8006 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4203..4210 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4203 
                     /label="NLRP12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTACGAACC GCAGGCAGGG ACGGCCTCTG TCGCCTGTCC ACCTACTTGG 
     1081 AAGAACTCGA GGCTGTGGAA CTGAAGAAGT TCAAGTTATA CCTGGGGACC GCGACAGAGC 
     1141 TGGGAGAAGG CAAGATCCCC TGGGGAAGCA TGGAGAAGGC CGGTCCCCTG GAAATGGCCC 
     1201 AGCTGCTCAT CACCCACTTC GGGCCAGAGG AGGCCTGGAG GTTGGCTCTC AGCACCTTTG 
     1261 AGCGGATAAA CAGGAAGGAC CTGTGGGAGA GAGGACAGAG AGAGGACCTG GTGAGGGATA 
     1321 CCCCACCTGG TGGCCCGTCC TCACTTGGGA ACCAGTCAAC ATGCCTTCTG GAAGTCTCTC 
     1381 TTGTCACTCC AAGAAAAGAT CCCCAGGAAA CCTACAGGGA CTATGTCCGC AGGAAATTCC 
     1441 GGCTCATGGA AGACCGCAAT GCGCGCCTAG GGGAATGTGT CAACCTCAGC CACCGGTACA 
     1501 CCCGGCTCCT GCTGGTGAAG GAGCACTCAA ACCCCATGCA GGTCCAGCAG CAGCTTCTGG 
     1561 ACACAGGCCG GGGACACGCG AGGACCGTGG GACACCAGGC TAGCCCCATC AAGATAGAGA 
     1621 CCCTCTTTGA GCCAGACGAG GAGCGCCCCG AGCCACCGCG CACCGTGGTC ATGCAAGGCG 
     1681 CGGCAGGGAT AGGCAAGTCC ATGCTGGCAC ACAAGGTGAT GCTGGACTGG GCGGACGGGA 
     1741 AGCTCTTCCA AGGCAGATTT GATTATCTCT TCTACATCAA CTGCAGGGAG ATGAACCAGA 
     1801 GTGCCACGGA ATGCAGCATG CAAGACCTCA TCTTCAGCTG CTGGCCTGAG CCCAGCGCGC 
     1861 CTCTCCAGGA GCTCATCCGA GTTCCCGAGC GCCTCCTTTT CATCATCGAC GGCTTCGATG 
     1921 AGCTCAAGCC TTCTTTCCAC GATCCTCAGG GACCCTGGTG CCTCTGCTGG GAGGAGAAAC 
     1981 GGCCCACGGA GCTGCTTCTT AACAGCTTAA TTCGGAAGAA GCTGCTCCCT GAGCTATCTT 
     2041 TGCTCATCAC CACACGGCCC ACGGCTTTGG AGAAGCTCCA CCGTCTGCTG GAGCACCCCA 
     2101 GGCATGTGGA GATCCTGGGC TTCTCTGAGG CAGAAAGGAA GGAATACTTC TACAAGTATT 
     2161 TCCACAATGC AGAGCAGGCG GGCCAAGTCT TCAATTACGT GAGGGACAAC GAGCCTCTCT 
     2221 TCACCATGTG CTTCGTCCCC CTGGTGTGCT GGGTGGTGTG TACCTGCCTC CAGCAGCAGC 
     2281 TGGAGGGTGG GGGGCTGTTG AGACAGACGT CCAGGACCAC CACTGCAGTG TACATGCTCT 
     2341 ACCTGCTGAG TCTGATGCAA CCCAAGCCGG GGGCCCCGCG CCTCCAGCCC CCACCCAACC 
     2401 AGAGAGGGTT GTGCTCCTTG GCGGCAGATG GGCTCTGGAA TCAGAAAATC CTATTTGAGG 
     2461 AGCAGGACCT CCGGAAGCAC GGCCTAGACG GGGAAGACGT CTCTGCCTTC CTCAACATGA 
     2521 ACATCTTCCA GAAGGACATC AACTGTGAGA GGTACTACAG CTTCATCCAC TTGAGTTTCC 
     2581 AGGAATTCTT TGCAGCTATG TACTATATCC TGGACGAGGG GGAGGGCGGG GCAGGCCCAG 
     2641 ACCAGGACGT GACCAGGCTG TTGACCGAGT ACGCGTTTTC TGAAAGGAGC TTCCTGGCAC 
     2701 TCACCAGCCG CTTCCTGTTT GGACTCCTGA ACGAGGAGAC CAGGAGCCAC CTGGAGAAGA 
     2761 GTCTCTGCTG GAAGGTCTCG CCGCACATCA AGATGGACCT GTTGCAGTGG ATCCAAAGCA 
     2821 AAGCTCAGAG CGACGGCTCC ACCCTGCAGC AGGGCTCCTT GGAGTTCTTC AGCTGCTTGT 
     2881 ACGAGATCCA GGAGGAGGAG TTTATCCAGC AGGCCCTGAG CCACTTCCAG GTGATCGTGG 
     2941 TCAGCAACAT TGCCTCCAAG ATGGAGCACA TGGTCTCCTC GTTCTGTCTG AAGCGCTGCA 
     3001 GGAGCGCCCA GGTGCTGCAC TTGTATGGCG CCACCTACAG CGCGGACGGG GAAGACCGCG 
     3061 CGAGGTGCTC CGCAGGAGCG CACACGCTGT TGGTGCAGCT ACCAGAGAGG ACCGTTCTGC 
     3121 TGGACGCCTA CAGTGAACAT CTGGCAGCGG CCCTGTGCAC CAATCCAAAC CTGATAGAGC 
     3181 TGTCTCTGTA CCGAAATGCC CTGGGCAGCC GGGGGGTGAA GCTGCTCTGT CAAGGACTCA 
     3241 GACACCCCAA CTGCAAACTT CAGAACCTGA GGCTGAAGAG GTGCCGCATC TCCAGCTCAG 
     3301 CCTGCGAGGA CCTCTCTGCA GCTCTCATAG CCAATAAGAA TTTGACAAGG ATGGATCTCA 
     3361 GTGGCAACGG CGTTGGATTC CCAGGCATGA TGCTGCTTTG CGAGGGCCTG CGGCATCCCC 
     3421 AGTGCAGGCT GCAGATGATT CAGTTGAGGA AGTGTCAGCT GGAGTCCGGG GCTTGTCAGG 
     3481 AGATGGCTTC TGTGCTCGGC ACCAACCCAC ATCTGGTTGA GTTGGACCTG ACAGGAAATG 
     3541 CACTGGAGGA TTTGGGCCTG AGGTTACTAT GCCAGGGACT GAGGCACCCA GTCTGCAGAC 
     3601 TACGGACTTT GTGGCTGAAG ATCTGCCGCC TCACTGCTGC TGCCTGTGAC GAGCTGGCCT 
     3661 CAACTCTCAG TGTGAACCAG AGCCTGAGAG AGCTGGACCT GAGCCTGAAT GAGCTGGGGG 
     3721 ACCTCGGGGT GCTGCTGCTG TGTGAGGGCC TCAGGCATCC CACGTGCAAG CTCCAGACCC 
     3781 TGCGGTTGGG CATCTGCCGG CTGGGCTCTG CCGCCTGTGA GGGTCTTTCT GTGGTGCTCC 
     3841 AGGCCAACCA CAACCTCCGG GAGCTGGACT TGAGTTTCAA CGACCTGGGA GACTGGGGCC 
     3901 TGTGGTTGCT GGCTGAGGGG CTGCAACATC CCGCCTGCAG ACTCCAGAAA CTGTGGCTGG 
     3961 ATAGCTGTGG CCTCACAGCC AAGGCTTGTG AGAATCTTTA CTTCACCCTG GGGATCAACC 
     4021 AGACCTTGAC CGACCTTTAC CTGACCAACA ACGCCCTAGG GGACACAGGT GTCCGACTGC 
     4081 TTTGCAAGCG GCTGAGCCAT CCTGGCTGCA AACTCCGAGT CCTCTGGTTA TTTGGGATGG 
     4141 ACCTGAATAA AATGACCCAC AGTAGGTTGG CAGCGCTTCG AGTAACAAAA CCTTATTTGG 
     4201 ACATTGGCTG CAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4261 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4321 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4381 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4441 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4501 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4561 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4621 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4681 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4741 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4801 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4861 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4921 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4981 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5821 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5881 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5941 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6001 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6061 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6121 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6181 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6241 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6301 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6361 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6421 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6481 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6541 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6601 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6661 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6721 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6781 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6841 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6901 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6961 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7021 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7081 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7141 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7201 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7261 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7321 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7501 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7561 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7621 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7681 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7741 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7801 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7861 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7921 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7981 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8041 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//