LOCUS       RC207013                8136 bp    DNA     circular  SYN 
DEFINITION  BUB1 (Myc-DDK-tagged)-Human budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1)
ACCESSION   NM_004336
VERSION     NM_004336.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4293..4384 
                     /label="Myc-DDK"  
     misc_feature    4414..4999 
                     /label="PolyA signal"  
     misc_feature    4471..4490 
                     /label="Restriction Enzyme"  
     misc_feature    5275..5947 
                     /label="ColE1"  
     misc_feature    6377..7171 
                     /label="Kan/Neo"  
     misc_feature    7206..7508 
                     /label="SV40 ori"  
     misc_feature    7594..8071 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4275..4281 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4275 
                     /label="BUB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACACCCCG GAAAATGTCC TTCAGATGCT TGAAGCCCAC ATGCAGAGCT 
     1081 ACAAGGGCAA TGACCCTCTT GGTGAATGGG AAAGATACAT ACAGTGGGTA GAAGAGAATT 
     1141 TTCCTGAGAA TAAAGAATAC TTGATAACTT TACTAGAACA TTTAATGAAG GAATTTTTAG 
     1201 ATAAGAAGAA ATACCACAAT GACCCAAGAT TCATCAGTTA TTGTTTAAAA TTTGCTGAGT 
     1261 ACAACAGTGA CCTCCATCAA TTTTTTGAGT TTCTGTACAA CCATGGGATT GGAACCCTGT 
     1321 CATCCCCTCT GTACATTGCC TGGGCGGGGC ATCTGGAAGC CCAAGGAGAG CTGCAGCATG 
     1381 CCAGTGCTGT CCTTCAGAGA GGAATTCAAA ACCAGGCTGA ACCCAGAGAG TTCCTGCAAC 
     1441 AACAATACAG GTTATTTCAG ACACGCCTCA CTGAAACCCA TTTGCCAGCT CAAGCTAGAA 
     1501 CCTCAGAACC TCTGCATAAT GTTCAGGTTT TAAATCAAAT GATAACATCA AAATCAAATC 
     1561 CAGGAAATAA CATGGCCTGC ATTTCTAAGA ATCAGGGTTC AGAGCTTTCT GGAGTGATAT 
     1621 CTTCAGCTTG TGATAAAGAG TCAAATATGG AACGAAGAGT GATCACGATT TCTAAATCAG 
     1681 AATATTCTGT GCACTCATCT TTGGCATCCA AAGTTGATGT TGAGCAGGTT GTTATGTATT 
     1741 GCAAGGAGAA GCTTATTCGT GGGGAATCAG AATTTTCCTT TGAAGAATTG AGAGCCCAGA 
     1801 AATACAATCA ACGGAGAAAG CATGAGCAAT GGGTAAATGA AGACAGACAT TATATGAAAA 
     1861 GGAAAGAAGC AAATGCTTTT GAAGAACAGC TATTAAAACA GAAAATGGAT GAACTTCATA 
     1921 AGAAGTTGCA TCAGGTGGTG GAGACATCCC ATGAGGATCT GCCCGCTTCC CAGGAAAGGT 
     1981 CCGAGGTTAA TCCAGCACGT ATGGGGCCAA GTGTAGGCTC CCAGCAGGAA CTGAGAGCGC 
     2041 CATGTCTTCC AGTAACCTAT CAGCAGACAC CAGTGAACAT GGAAAAGAAC CCAAGAGAGG 
     2101 CACCTCCTGT TGTTCCTCCT TTGGCAAATG CTATTTCTGC AGCTTTGGTG TCCCCAGCCA 
     2161 CCAGCCAGAG CATTGCTCCT CCTGTTCCTT TGAAAGCCCA GACAGTAACA GACTCCATGT 
     2221 TTGCAGTGGC CAGCAAAGAT GCTGGATGTG TGAATAAGAG TACTCATGAA TTCAAGCCAC 
     2281 AGAGTGGAGC AGAGATCAAA GAAGGGTGTG AAACACATAA GGTTGCCAAC ACAAGTTCTT 
     2341 TTCACACAAC TCCAAACACA TCACTGGGAA TGGTTCAGGC AACGCCATCC AAAGTGCAGC 
     2401 CATCACCCAC CGTGCACACA AAAGAAGCAT TAGGTTTCAT CATGAATATG TTTCAGGCTC 
     2461 CTACACTTCC TGATATTTCT GATGACAAAG ATGAATGGCA ATCTCTAGAT CAAAATGAAG 
     2521 ATGCATTTGA AGCCCAGTTT CAAAAAAATG TAAGGTCATC TGGGGCTTGG GGAGTCAATA 
     2581 AGATCATCTC TTCTTTGTCA TCTGCTTTTC ATGTGTTTGA AGATGGAAAC AAAGAAAATT 
     2641 ATGGATTACC ACAGCCTAAA AATAAACCCA CAGGAGCCAG GACCTTTGGA GAACGCTCTG 
     2701 TCAGCAGACT TCCTTCAAAA CCAAAGGAGG AAGTGCCTCA TGCTGAAGAG TTTTTGGATG 
     2761 ACTCAACTGT ATGGGGTATT CGCTGCAACA AAACCCTGGC ACCCAGTCCT AAGAGCCCAG 
     2821 GAGACTTCAC ATCTGCTGCA CAACTTGCGT CTACACCATT CCACAAGCTT CCAGTGGAGT 
     2881 CAGTGCACAT TTTAGAAGAT AAAGAAAATG TGGTAGCAAA ACAGTGTACC CAGGCGACTT 
     2941 TGGATTCTTG TGAGGAAAAC ATGGTGGTGC CTTCAAGGGA TGGAAAATTC AGTCCAATTC 
     3001 AAGAGAAAAG CCCAAAACAG GCCTTGTCGT CTCACATGTA TTCAGCATCC TTACTTCGTC 
     3061 TGAGCCAGCC TGCTGCAGGT GGGGTACTTA CCTGTGAGGC AGAGTTGGGC GTTGAGGCTT 
     3121 GCAGACTCAC AGACACTGAC GCTGCCATTG CAGAAGATCC ACCAGATGCT ATTGCTGGGC 
     3181 TCCAAGCAGA ATGGATGCAG ATGAGTTCAC TTGGGACTGT TGATGCTCCA AACTTCATTG 
     3241 TTGGGAACCC ATGGGATGAT AAGCTGATTT TCAAACTTTT ATCTGGGCTT TCTAAACCAG 
     3301 TGAGTTCCTA TCCAAATACT TTTGAATGGC AATGTAAACT TCCAGCCATC AAGCCCAAGA 
     3361 CTGAATTTCA ATTGGGTTCT AAGCTGGTCT ATGTCCATCA CCTTCTTGGA GAAGGAGCCT 
     3421 TTGCCCAGGT GTACGAAGCT ACCCAGGGAG ATCTGAATGA TGCTAAAAAT AAACAGAAAT 
     3481 TTGTTTTAAA GGTCCAAAAG CCTGCCAACC CCTGGGAATT CTACATTGGG ACCCAGTTGA 
     3541 TGGAAAGACT AAAGCCATCT ATGCAGCACA TGTTTATGAA GTTCTATTCT GCCCACTTAT 
     3601 TCCAGAATGG CAGTGTATTA GTAGGAGAGC TCTACAGCTA TGGAACATTA TTAAATGCCA 
     3661 TTAACCTCTA TAAAAATACC CCTGAAAAAG TGATGCCTCA AGGTCTTGTC ATCTCTTTTG 
     3721 CTATGAGAAT GCTTTACATG ATTGAGCAAG TGCATGACTG TGAAATCATT CATGGAGACA 
     3781 TTAAACCAGA CAATTTCATA CTTGGAAACG GATTTTTGGA ACAGGATGAT GAAGATGATT 
     3841 TATCTGCTGG CTTGGCACTG ATTGACCTGG GTCAGAGTAT AGATATGAAA CTTTTTCCAA 
     3901 AAGGAACTAT ATTCACAGCA AAGTGTGAAA CATCTGGTTT TCAGTGTGTT GAGATGCTCA 
     3961 GCAACAAACC ATGGAACTAC CAGATCGATT ACTTTGGGGT TGCTGCAACA GTATATTGCA 
     4021 TGCTCTTTGG CACTTACATG AAAGTGAAAA ATGAAGGAGG AGAGTGTAAG CCTGAAGGTC 
     4081 TTTTTAGAAG GCTTCCTCAT TTGGATATGT GGAATGAATT TTTTCATGTT ATGTTGAATA 
     4141 TTCCAGATTG TCATCATCTT CCATCTTTGG ATTTGTTAAG GCAAAAGCTG AAGAAAGTAT 
     4201 TTCAACAACA CTATACTAAC AAGATTAGGG CCCTACGTAA TAGGCTAATT GTACTGCTCT 
     4261 TAGAATGTAA GCGTTCACGA AAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4321 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4381 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4441 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4501 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4561 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4621 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4681 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4741 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4801 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4861 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4921 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4981 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5041 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5101 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5161 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5221 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5281 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5341 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5401 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5461 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5521 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5581 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5641 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5701 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5761 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5821 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5881 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5941 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6001 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6061 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6121 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6181 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6241 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6301 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6361 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6421 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6481 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6541 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6601 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6661 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6721 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6781 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6841 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6901 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6961 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7021 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7081 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7141 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7201 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7261 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7321 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7381 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7561 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7621 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7681 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7741 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7801 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7861 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7921 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7981 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8041 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8101 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//