LOCUS       RC206992                7311 bp    DNA     circular  SYN 
DEFINITION  TMEM63A (Myc-DDK-tagged)-Human transmembrane protein 63A (TMEM63A)
ACCESSION   NM_014698
VERSION     NM_014698.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3466..3557 
                     /label="Myc-DDK"  
     misc_feature    3587..4172 
                     /label="PolyA signal"  
     misc_feature    3644..3663 
                     /label="Restriction Enzyme"  
     misc_feature    4448..5120 
                     /label="ColE1"  
     misc_feature    5550..6344 
                     /label="Kan/Neo"  
     misc_feature    6379..6681 
                     /label="SV40 ori"  
     misc_feature    6767..7244 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3441..3448 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3441 
                     /label="TMEM63A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGACTCC CCGTTCCTGG AGCTGTGGCA GTCCAAGGCA GTGTCCATCA 
     1081 GGGAGCAGCT GGGACTCGGG GACCGGCCCA ACGACTCCTA TTGCTACAAC TCGGCCAAAA 
     1141 ACAGCACCGT GCTCCAGGGG GTCACCTTTG GTGGCATCCC CACTGTCCTG CTCATAGACG 
     1201 TCAGCTGCTT CCTGTTCTTA ATCTTGGTGT TTTCTATTAT AAGAAGAAGA TTCTGGGACT 
     1261 ATGGCCGCAT TGCCCTGGTG TCAGAAGCAG ACAGCGAGTC CAGATTTCAG AGATTGTCAT 
     1321 CGACTTCCTC CTCAGGTCAA CAAGACTTTG AAAATGAGCT GGGATGCTGT CCCTGGCTGA 
     1381 CTGCCATCTT CCGTCTGCAT GATGACCAGA TCCTGGAATG GTGTGGGGAG GACGCCATCC 
     1441 ACTACCTGTC CTTCCAGAGG CACATCATCT TCCTGTTGGT GGTGGTCAGC TTTTTGTCCC 
     1501 TGTGTGTCAT CCTGCCTGTC AACCTCTCAG GGGACTTGCT GGACAAAGAC CCGTATAGTT 
     1561 TTGGGAGGAC AACAATAGCA AACCTACAGA CTGACAATGA CCTCCTTTGG CTGCACACCA 
     1621 TCTTTGCTGT CATTTACCTC TTCCTCACTG TGGGTTTCAT GCGGCACCAC ACTCAGTCCA 
     1681 TTAAGTACAA AGAGGAGAAC CTGGTGAGGC GGACCCTGTT CATCACAGGA CTCCCCAGAG 
     1741 ATGCCAGGAA GGAGACTGTG GAGAGCCACT TCCGGGACGC GTATCCCACG TGTGAGGTGG 
     1801 TTGATGTGCA GCTGTGCTAC AACGTGGCCA AACTGATCTA CCTGTGCAAG GAGAAAAAGA 
     1861 AGACTGAGAA GAGCCTGACC TATTACACAA ACCTGCAGGT GAAGACAGGC CAGCGGACCC 
     1921 TCATCAACCC CAAGCCCTGT GGCCAGTTTT GCTGCTGTGA AGTGCTGGGC TGTGAGTGGG 
     1981 AAGACGCCAT CTCTTACTAC ACACGGATGA AGGACAGGCT GCTGGAGAGG ATCACAGAGG 
     2041 AAGAACGCCA CGTCCAGGAC CAGCCCCTGG GAATGGCCTT CGTCACCTTC CAGGAGAAGT 
     2101 CCATGGCCAC CTACATCCTG AAAGATTTCA ATGCCTGCAA GTGTCAGAGC CTTCAGTGCA 
     2161 AAGGTGAGCC CCAGCCGTCC TCCCATAGCA GGGAGCTCTA TACCTCCAAG TGGACAGTCA 
     2221 CCTTTGCTGC TGACCCTGAG GACATCTGCT GGAAGAACCT CTCTATCCAG GGCCTCCGCT 
     2281 GGTGGCTACA GTGGCTGGGC ATCAACTTCA CCCTCTTCCT GGGGCTATTT TTCCTGACCA 
     2341 CACCCTCCAT CATCCTGTCC ACCATGGACA AGTTTAATGT CACCAAACCC ATCCATGCGC 
     2401 TGAATAACCC GATCATCAGC CAGTTCTTCC CCACCCTCCT GCTCTGGTCC TTCTCGGCCC 
     2461 TGCTCCCCTC CATTGTCTAC TACTCTACAC TGCTGGAGTC TCACTGGACC AAGTCGGGGG 
     2521 AAAACCAGAT CATGATGACC AAAGTCTACA TATTCTTGAT CTTCATGGTG CTGATCCTGC 
     2581 CCTCCCTGGG TCTCACCAGT CTAGATTTTT TCTTCCGGTG GCTCTTTGAC AAAACTTCCT 
     2641 CGGAGGCCTC CATCAGGTTG GAGTGCGTCT TCCTGCCTGA CCAGGGTGCC TTCTTTGTGA 
     2701 ACTATGTCAT CGCCTCGGCC TTCATCGGCA ATGGCATGGA GCTGCTGCGG CTGCCAGGTC 
     2761 TCATCCTCTA TACCTTCCGC ATGATCATGG CCAAGACGGC TGCTGACCGC AGGAATGTCA 
     2821 AGCAGAACCA GGCCTTCCAG TACGAGTTTG GAGCCATGTA TGCATGGATG CTGTGTGTCT 
     2881 TCACTGTCAT CGTGGCCTAC AGCATCACTT GTCCCATCAT CGCGCCATTT GGCCTCATCT 
     2941 ACATCCTGCT CAAGCACATG GTGGACCGGC ACAACCTCTA CTTCGTCTAC CTCCCAGCCA 
     3001 AGCTGGAGAA GGGGATCCAC TTTGCCGCTG TGAACCAGGC CTTGGCAGCC CCCATCCTGT 
     3061 GCCTCTTCTG GCTCTACTTC TTTTCCTTCC TGCGCCTGGG TATGAAGGCC CCCGCCACTC 
     3121 TGTTCACCTT CCTGGTGCTG CTGCTCACCA TCCTGGTCTG CCTGGCTCAC ACCTGCTTTG 
     3181 GATGCTTCAA GCACCTCAGC CCTCTGAACT ACAAAACAGA AGAGCCTGCA AGTGACAAAG 
     3241 GAAGTGAGGC AGAGGCCCAC ATGCCCCCAC CGTTCACACC CTACGTGCCT CGGATTCTGA 
     3301 ACGGCTTGGC CTCGGAGAGG ACAGCACTGT CTCCGCAGCA GCAGCAGCAG CAGACCTATG 
     3361 GTGCCATCCA CAACATCAGC GGGACTATCC CTGGACAGTG CTTGGCGCAG AGCGCCACGG 
     3421 GCAGTGTGGC TGCTGCCCCC CAGGAGGCCA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     3481 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3541 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3601 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3661 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3721 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3781 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3841 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3901 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3961 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4021 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4081 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4141 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4201 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4261 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4321 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4381 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4441 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4501 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4561 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4621 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4681 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4741 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4801 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4861 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4921 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4981 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5041 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5101 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5161 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5221 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5281 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5341 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5401 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5461 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5521 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5581 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5641 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5701 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5761 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5821 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5881 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5941 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6001 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6061 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6121 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6181 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6241 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6301 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6361 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6421 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6481 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6541 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6601 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6661 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6721 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6781 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6841 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6901 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6961 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7021 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7081 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7141 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7201 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7261 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//