LOCUS RC206970L3 8798 bp DNA circular SYN DEFINITION PINK1 (Myc-DDK-tagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein ACCESSION NM_032409 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 7089..7269 /label="3-LTR" misc_feature 833..1013 /label="5-LTR" misc_feature 7997..8656 /label="CAT(Cam-r)" misc_feature 2620..3234 /label="CMV" misc_feature 7317..7936 /label="ColE1" misc_feature 5129..5152 /label="DDK" misc_feature 5964..5984 /label="LR50" misc_feature 5081..5152 /label="MYC" misc_feature 5162..5227 /label="P2A" misc_feature 5228..5827 /label="Puro" misc_feature 3252..3271 /label="T7" misc_feature 3221..3237 /label="V2" misc_feature 5973..6564 /label="WPRE" misc_feature 3320..3328 /label="Restriction Enzyme" misc_feature 5063..5069 /label="Restriction Enzyme" misc_feature 3320..5063 /label="PINK1" ORIGIN 1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGTGCGA CAGGCGCTGG GCCGCGGCCT 3361 GCAGCTGGGT CGAGCGCTGC TGCTGCGCTT CACGGGCAAG CCCGGCCGGG CCTACGGCTT 3421 GGGGCGGCCG GGCCCGGCGG CGGGCTGTGT CCGCGGGGAG CGTCCAGGCT GGGCCGCAGG 3481 ACCGGGCGCG GAGCCTCGCA GGGTCGGGCT CGGGCTCCCT AACCGTCTCC GCTTCTTCCG 3541 CCAGTCGGTG GCCGGGCTGG CGGCGCGGTT GCAGCGGCAG TTCGTGGTGC GGGCCTGGGG 3601 CTGCGCGGGC CCTTGCGGCC GGGCAGTCTT TCTGGCCTTC GGGCTAGGGC TGGGCCTCAT 3661 CGAGGAAAAA CAGGCGGAGA GCCGGCGGGC GGTCTCGGCC TGTCAGGAGA TCCAGGCAAT 3721 TTTTACCCAG AAAAGCAAGC CGGGGCCTGA CCCGTTGGAC ACGAGACGCT TGCAGGGCTT 3781 TCGGCTGGAG GAGTATCTGA TAGGGCAGTC CATTGGTAAG GGCTGCAGTG CTGCTGTGTA 3841 TGAAGCCACC ATGCCTACAT TGCCCCAGAA CCTGGAGGTG ACAAAGAGCA CCGGGTTGCT 3901 TCCAGGGAGA GGCCCAGGTA CCAGTGCACC AGGAGAAGGG CAGGAGCGAG CTGCGGGGGC 3961 CCCTGCCTTC CCCTTGGCCA TCAAGATGAT GTGGAACATC TCGGCAGGTT CCTCCAGCGA 4021 AGCCATCTTG AACACAATGA GCCAGGAGCT GGTCCCAGCG AGCCGAGTGG CCTTGGCTGG 4081 GGAGTATGGA GCAGTCACTT ACAGAAAATC CAAGAGAGGT CCCAAGCAAC TAGCCCCTCA 4141 CCCCAACATC ATCCGGGTTC TCCGCGCCTT CACCTCTTCC GTGCCGCTGC TGCCAGGGGC 4201 CCTGGTCGAC TACCCTGATG TGCTGCCCTC ACGCCTCCAC CCTGAAGGCC TGGGCCATGG 4261 CCGGACGCTG TTCCTCGTTA TGAAGAACTA TCCCTGTACC CTGCGCCAGT ACCTTTGTGT 4321 GAACACACCC AGCCCCCGCC TCGCCGCCAT GATGCTGCTG CAGCTGCTGG AAGGCGTGGA 4381 CCATCTGGTT CAACAGGGCA TCGCGCACAG AGACCTGAAA TCCGACAACA TCCTTGTGGA 4441 GCTGGACCCA GACGGCTGCC CCTGGCTGGT GATCGCAGAT TTTGGCTGCT GCCTGGCTGA 4501 TGAGAGCATC GGCCTGCAGT TGCCCTTCAG CAGCTGGTAC GTGGATCGGG GCGGAAACGG 4561 CTGTCTGATG GCCCCAGAGG TGTCCACGGC CCGTCCTGGC CCCAGGGCAG TGATTGACTA 4621 CAGCAAGGCT GATGCCTGGG CAGTGGGAGC CATCGCCTAT GAAATCTTCG GGCTTGTCAA 4681 TCCCTTCTAC GGCCAGGGCA AGGCCCACCT TGAAAGCCGC AGCTACCAAG AGGCTCAGCT 4741 ACCTGCACTG CCCGAGTCAG TGCCTCCAGA CGTGAGACAG TTGGTGAGGG CACTGCTCCA 4801 GCGAGAGGCC AGCAAGAGAC CATCTGCCCG AGTAGCCGCA AATGTGCTTC ATCTAAGCCT 4861 CTGGGGTGAA CATATTCTAG CCCTGAAGAA TCTGAAGTTA GACAAGATGG TTGGCTGGCT 4921 CCTCCAACAA TCGGCCGCCA CTTTGTTGGC CAACAGGCTC ACAGAGAAGT GTTGTGTGGA 4981 AACAAAAATG AAGATGCTCT TTCTGGCTAA CCTGGAGTGT GAAACGCTCT GCCAGGCAGC 5041 CATCCTCCTC TGCTCATGGA GGGCAGCCCT GACGCGTACG CGGCCGCTCG AGCAGAAACT 5101 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 5161 GGTTTGGGTA GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA 5221 GGAGAACCCT GGACCTATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA 5281 CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA 5341 CACCGTCGAT CCGGATCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC 5401 GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT 5461 CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT 5521 GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC 5581 GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA 5641 GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG 5701 GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG 5761 CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG 5821 CAAGCCCGGT GCCTGATGTA CACCCAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG 5881 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT 5941 TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA 6001 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 6061 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 6121 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 6181 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 6241 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 6301 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 6361 TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA 6421 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 6481 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 6541 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA 6601 GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG 6661 CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA 6721 CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG 6781 GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA 6841 CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG 6901 ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT 6961 GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG 7021 AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG 7081 CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT 7141 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA 7201 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA 7261 GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 7321 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 7381 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 7441 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 7501 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 7561 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 7621 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 7681 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 7741 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 7801 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 7861 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 7921 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 7981 TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT 8041 GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA 8101 TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG 8161 GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG 8221 GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT 8281 CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT 8341 ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT 8401 GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG 8461 CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT 8521 TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG 8581 CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG 8641 TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 8701 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 8761 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC //