LOCUS       RC206955                8928 bp    DNA     circular  SYN 
DEFINITION  EFCAB6 (Myc-DDK-tagged)-Human EF-hand calcium binding domain 6 (EFCAB6), transcript variant 2
ACCESSION   NM_198856
VERSION     NM_198856.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5085..5176 
                     /label="Myc-DDK"  
     misc_feature    5206..5791 
                     /label="PolyA signal"  
     misc_feature    5263..5282 
                     /label="Restriction Enzyme"  
     misc_feature    6067..6739 
                     /label="ColE1"  
     misc_feature    7169..7963 
                     /label="Kan/Neo"  
     misc_feature    7998..8300 
                     /label="SV40 ori"  
     misc_feature    8386..8863 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5067..5073 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5067 
                     /label="EFCAB6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATTGCTGC CGCACATTAA GAGAACTTGA AATCCAAGTG GGAGAAAAGG 
     1081 TTTTCAAAAA CATTAAGACT GTTATGAAAG CCTTTGAGCT CATTGATGTT AACAAGACTG 
     1141 GACTGGTTCG ACCGCAGGAG CTAAGAAGGG TTCTGGAGAC CTTCTGTATG AAGTTAAGAG 
     1201 ACGAGGAATA CGAAAAGTTT TCGAAACACT ACAACATCCA CAAGGATACT GCAGTAGATT 
     1261 ACAACGTGTT TTTGAAGAAT CTCAGCATAA ATAACGACTT GAACCTTAGA TATTGTATGG 
     1321 GAAATCAAGA GGTCTCATTG GAGAATCAAC AAGCCAAAAA TTCCAAAAAG GAACGTTTGC 
     1381 TAGGTTCTGC ATCATCTGAA GATATCTGGA GAAACTACTC CTTGGATGAA ATTGAGAGGA 
     1441 ACTTTTGTCT ACAACTTTCG AAGTCTTATG AAAAGGTTGA AAAGGCCCTC AGTGCAGGGG 
     1501 ACCCCTGTAA AGGTGGCTAC GTGTCTTTTA ATTATCTAAA GATTGTCCTC GACACTTTTG 
     1561 TATACCAAAT ACCAAGAAGA ATTTTTATCC AGTTAATGAA AAGATTTGGA CTTAAAGCCA 
     1621 CCACTAAAAT CAATTGGAAG CAATTTCTAA CATCATTTCA TGAGCCTCAG GGGTTGCAAG 
     1681 TTAGCAGTAA AGGTCCCCTG ACAAAAAGAA ATAGCATCAA CTCTAGAAAT GAATCTCACA 
     1741 AGGAAAACAT CATCACAAAG TTATTTAGAC ACACTGAAGA TCACTCTGCG TCACTGAAGA 
     1801 AAGCATTACT GATAATCAAC ACTAAACCCG ATGGACCGAT AACAAGAGAA GAATTTCGAT 
     1861 ATATTCTAAA TTGCATGGCT GTAAAACTAA GCGATTCAGA ATTCAAAGAA CTAATGCAAA 
     1921 TGCTTGACCC TGGGGACACT GGAGTGGTCA ACACCAGCAT GTTTATTGAT CTGATTGAAG 
     1981 AGAACTGTAG GATGAGAAAG ACATCTCCCT GTACAGATGC TAAGACACCT TTCCTCCTGG 
     2041 CCTGGGATTC AGTGGAAGAA ATTGTTCATG ATACAATTAC TAGGAACCTA CAAGCTTTTT 
     2101 ATAACATGCT ACGCTCATAT GACCTTGGAG ACACAGGGCG CATTGGCCGA AATAATTTCA 
     2161 AGAAAATCAT GCACGTCTTC TGTCCATTTT TAACGAATGC ACATTTCATA AAACTCTGCA 
     2221 GTAAGATTCA GGACATTGGT TCAGGAAGAA TCCTTTACAA GAAACTTTTG GCATGCATAG 
     2281 GAATTGATGG CCCACCCACT GTCTCTCCAG TTCTTGTTCC AAAGGATCAG CTGTTAAGTG 
     2341 AACATTTACA AAAAGATGAA CAGCAGCAGC CAGATCTTTC TGAGAGAACC AAGCTCACGG 
     2401 AGGATAAAAC CACCCTGACC AAGAAGATGA CCACAGAAGA AGTGATTGAA AAATTCAAAA 
     2461 AGTGTATACA GCAGCAGGAC CCGGCATTCA AAAAACGATT TCTTGACTTC AGCAAGGAGC 
     2521 CTAATGGAAA AATTAACGTG CATGACTTTA AGAAGGTACT GGAAGACACT GGGATGCCCA 
     2581 TGGACGATGA TCAGTATGCC CTGCTGACCA CTAAAATAGG CTTCGAGAAG GAAGGGATGA 
     2641 GCTATCTTGA TTTTGCAGCA GGATTTGAAG ATCCTCCAAT GAGAGGGCCG GAAACCACTC 
     2701 CGCCGCAGCC TCCAACTCCT TCAAAAAGTT ACGTGAACAG CCACTTTATC ACCGCAGAAG 
     2761 AATGCCTGAA GCTTTTCCCT AGGAGGCTGA AGGAGTCATT CCGGGACCCC TACTCTGCCT 
     2821 TCTTTAAAAC AGATGCTGAC AGGGATGGCA TAATCAACAT GCATGACCTT CACAGACTGC 
     2881 TCCTGCATCT ACTGCTTAAT CTCAAAGACG ACGAGTTTGA GCGCTTCCTT GGCCTTCTTG 
     2941 GCTTGAGACT TAGTGTCACT TTAAATTTTC GGGAATTTCA AAATTTGTGT GAGAAGAGAC 
     3001 CATGGAGAAC AGATGAAGCG CCTCAAAGAC TCATTAGACC AAAACAGAAG GTTGCGGATT 
     3061 CAGAACTAGC TTGTGAGCAG GCTCATCAGT ATCTTGTTAC CAAAGCAAAA AACAGATGGT 
     3121 CAGACTTGTC TAAGAATTTT CTAGAAACCG ATAATGAGGG CAATGGCATT CTTCGACGCC 
     3181 GGGACATAAA GAACGCACTG TACGGTTTTG ATATTCCCCT CACACCAAGA GAGTTTGAAA 
     3241 AGCTTTGGGC AAGATACGAC ACCGAGGGAA AAGGGCACAT TACTTACCAG GAATTTTTAC 
     3301 AGAAATTGGG TATTAACTAT TCGCCTGCTG TCCATCGGCC CTGTGCAGAG GATTATTTCA 
     3361 ACTTCATGGG TCATTTTACA AAGCCACAGC AGCTACAGGA AGAGATGAAG GAGCTGCAGC 
     3421 AGAGCACAGA GAAGGCTGTG GCAGCCAGGG ATAAGCTTAT GGACCGCCAT CAAGATATCA 
     3481 GCAAAGCATT CACCAAAACT GATCAATCCA AAACCAACTA CATATCCATA TGCAAGATGC 
     3541 AGGAAGTGCT GGAAGAATGT GGATGTTCTC TTACCGAAGG GGAGCTGACC CATCTGCTAA 
     3601 ACAGTTGGGG AGTCAGCCGG CATGATAATG CTATCAATTA CCTCGACTTC CTGAGAGCAG 
     3661 TGGAGAACAG CAAGTCAACA GGAGCTCAGC CCAAGGAAAA AGAAGAGAGC ATGCCAATCA 
     3721 ATTTTGCAAC GCTGAATCCA CAGGAGGTTG TGAGGAAGAT CCAGGAAGTA GTTGAGTCCT 
     3781 CCCAGCTGGC TTTGTCCACG GCATTTTCTG CATTGGGTAA AGAGGATACA GGATTTGTAA 
     3841 AGGCTACAGA ATTCGGACAA GTTCTTAAGG ATTTCTGTTA CAAACTAACG GACAATCAGT 
     3901 ATCATTATTT TTTGAGGAAA CTAAGAATTC ATCTAACCCC CTATATAAAT TGGAAATATT 
     3961 TTCTCCAGAA CTTTAGCTGT TTCCTTGAGG AGACAGCTGA TGAGTGGGCT GAGAAAATGC 
     4021 CCAAAGGCCC GCCGCCTACC TCTCCCAAGG CCACAGCCGA CAGAGACATC CTGGCTCGCC 
     4081 TCCACAAAGC AGTGACTTCC CATTACCATG CCATCACCCA GGAGTTTGAG AATTTTGACA 
     4141 CCATGAAAAC GAACACCATC TCCAGAGAGG AGTTTAGGGC CATTTGTAAT CGCCGCGTCC 
     4201 AAATCCTGAC GGACGAACAG TTTGGCAGAC TCTGGAACGA GATGCCAGTC AATGCCAAGG 
     4261 GGAGGCTGAA ATACCCGGAC TTCCTGAGCA GGTTCAGTTC CGAGACAGCA GCCACACCAA 
     4321 TGGCCACTGG TGACTCGGCC GTGGCCCAGA GAGGGAGCAG TGTCCCTGAC GTCTCGGAAG 
     4381 GCACCAGATC TGCCCTCTCA TTGCCCACTC AGGAGCTGAG ACCAGGGTCA AAGTCGCAGA 
     4441 GCCACCCCTG TACTCCAGCG AGCACCACCG TGATCCCGGG CACTCCACCC TTGCAGAACT 
     4501 GTGATCCCAT AGAGAGCAGG CTCCGGAAGA GAATCCAGGG CTGCTGGCGC CAGCTCCTGA 
     4561 AAGAATGCAA GGAGAAGGAC GTGGCCAGAC AGGGGGACAT CAACGCCTCC GATTTCCTGG 
     4621 CTCTTGTGGA GAAATTCAAC CTGGACATAA GCAAAGAGGA GTGTCAGCAG CTCATTATAA 
     4681 AATACGACTT AAAGAGCAAC GGGAAATTTG CATACTGTGA CTTCATTCAG AGCTGTGTCC 
     4741 TCCTGCTAAA AGCAAAGGAA AGCTCACTGA TGCACAGGAT GAAGATCCAG AATGCACACA 
     4801 AGATGAAAGA AGCCGGCGCG GAGACGCCAT CTTTTTACTC TGCTCTGCTG CGTATTCAGC 
     4861 CCAAAATTGT GCACTGCTGG AGGCCAATGC GGCGCACGTT CAAAAGCTAT GATGAGGCTG 
     4921 GAACAGGGCT GCTAAGCGTC GCAGATTTCA GGACGGTCCT GAGACAGTAC AGCATCAACC 
     4981 TCTCTGAGGA AGAGTTCTTC CATATTCTGG AGTATTACGA TAAGACGCTG TCTTCAAAAA 
     5041 TCTCCTACAA CGACTTCCTC CGGGCATTCC TCCAGACGCG TACGCGGCCG CTCGAGCAGA 
     5101 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5161 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5221 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5281 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5341 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5401 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5461 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5521 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5581 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5641 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5701 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5761 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5821 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5881 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5941 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6001 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6061 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6121 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6181 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6241 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6301 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6361 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6421 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6481 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6541 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6601 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6661 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6721 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6781 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6841 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6901 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6961 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7021 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7081 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7141 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7201 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7261 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7321 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7381 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7441 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7501 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7561 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7621 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7681 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7741 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7801 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7861 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7921 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7981 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8041 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8101 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8161 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8221 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8281 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8341 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8401 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8461 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8521 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8581 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8641 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8701 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8761 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8821 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8881 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//