LOCUS       RC206935                7620 bp    DNA     circular  SYN 
DEFINITION  CUL4B (Myc-DDK-tagged)-Human cullin 4B (CUL4B), transcript variant 1
ACCESSION   NM_003588
VERSION     NM_003588.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3777..3868 
                     /label="Myc-DDK"  
     misc_feature    3898..4483 
                     /label="PolyA signal"  
     misc_feature    3955..3974 
                     /label="Restriction Enzyme"  
     misc_feature    4759..5431 
                     /label="ColE1"  
     misc_feature    5861..6655 
                     /label="Kan/Neo"  
     misc_feature    6690..6992 
                     /label="SV40 ori"  
     misc_feature    7078..7555 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3759..3765 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3759 
                     /label="CUL4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTCACAG TCATCTGGAT CAGGAGATGG GAATGATGAT GAGGCTACTA 
     1081 CCTCTAAAGA CGGTGGTTTT TCTTCCCCCA GTCCCTCAGC TGCTGCTGCT GCTCAGGAGG 
     1141 TCAGATCTGC CACTGATGGT AATACCAGCA CCACTCCGCC CACCTCTGCC AAGAAGAGAA 
     1201 AGTTAAACAG CAGCAGCAGT AGCAGCAGTA ACAGTAGTAA CGAGAGAGAA GACTTTGATT 
     1261 CCACCTCTTC CTCCTCTTCC ACTCCTCCTT TACAACCCAG GGATTCGGCA TCCCCTTCAA 
     1321 CCTCGTCCTT CTGCCTGGGG GTTTCAGTGG CTGCTTCCAG CCACGTACCG ATACAGAAGA 
     1381 AGCTGCGTTT TGAAGACACC CTGGAGTTTG TAGGGTTTGA TGCGAAGATG GCTGAGGAAT 
     1441 CCTCCTCCTC CTCCTCCTCA TCTTCACCAA CTGCTGCAAC ATCTCAGCAG CAGCAACTTA 
     1501 AAAATAAGAG TATATTAATC TCTTCTGTGG CTTCGGTGCA TCATGCAAAC GGCCTAGCCA 
     1561 AATCTTCTAC CACCGTCTCT AGCTTTGCTA ACAGCAAACC TGGCTCTGCT AAGAAGTTAG 
     1621 TGATCAAGAA CTTTAAAGAT AAGCCTAAAT TACCAGAAAA CTACACAGAT GAAACCTGGC 
     1681 AAAAACTGAA AGAAGCAGTG GAAGCTATTC AGAATAGTAC TTCAATTAAG TACAATTTAG 
     1741 AAGAACTCTA CCAGGCTGTA GAAAATCTCT GTTCTTACAA GATTTCTGCA AACTTGTACA 
     1801 AACAGCTGAG ACAGATCTGC GAAGATCACA TCAAAGCACA GATTCATCAA TTCAGAGAGG 
     1861 ATTCATTGGA TAGCGTTCTT TTTTTAAAGA AGATTGATAG ATGCTGGCAA AACCATTGCA 
     1921 GACAAATGAT CATGATCAGG AGCATTTTTT TGTTTCTGGA TAGAACTTAC GTTCTTCAGA 
     1981 ATTCAATGCT ACCCTCCATT TGGGACATGG GACTGGAGTT ATTTAGGGCT CATATTATAA 
     2041 GTGATCAGAA AGTGCAGAAT AAGACAATTG ATGGCATTCT TCTCTTGATT GAGAGGGAAA 
     2101 GGAATGGTGA AGCAATTGAT AGAAGTTTAC TTCGAAGCCT TTTAAGCATG CTGTCTGATT 
     2161 TGCAAATTTA TCAAGATTCT TTTGAACAAC GATTTTTGGA AGAAACTAAC CGGCTCTATG 
     2221 CAGCTGAAGG CCAAAAATTA ATGCAAGAAA GAGAGGTTCC TGAATATCTA CATCATGTTA 
     2281 ACAAACGTCT AGAAGAAGAA GCAGACAGAC TTATTACTTA CTTAGATCAG ACCACCCAGA 
     2341 AGTCATTAAT TGCTACTGTA GAAAAACAAC TTCTAGGTGA ACACTTAACA GCAATTCTTC 
     2401 AGAAAGGTTT AAATAACCTC CTTGATGAAA ACCGAATTCA AGATTTGTCT CTTCTGTATC 
     2461 AGCTCTTCAG TAGAGTTCGA GGTGGAGTTC AGGTTCTTTT GCAGCAGTGG ATCGAATATA 
     2521 TCAAGGCATT TGGCAGCACT ATTGTAATTA ATCCTGAAAA AGATAAAACC ATGGTTCAAG 
     2581 AATTGCTGGA TTTTAAAGAT AAGGTTGACC ATATAATTGA TATCTGCTTT CTGAAGAATG 
     2641 AGAAATTTAT CAATGCCATG AAAGAAGCAT TTGAAACGTT CATTAACAAA AGACCAAATA 
     2701 AACCAGCTGA ACTTATAGCT AAGTATGTAG ATTCAAAACT TCGTGCAGGC AACAAAGAAG 
     2761 CTACAGATGA AGAACTTGAG AAAATGTTGG ATAAAATTAT GATCATATTT AGATTTATCT 
     2821 ATGGCAAGGA TGTTTTTGAG GCCTTCTATA AGAAAGATTT AGCCAAGCGC CTGTTAGTCG 
     2881 GAAAGAGTGC ATCTGTAGAT GCTGAAAAAT CAATGCTGTC CAAACTTAAA CATGAATGCG 
     2941 GAGCTGCTTT CACCAGCAAA CTTGAAGGAA TGTTTAAAGA CATGGAACTT TCTAAAGACA 
     3001 TCATGATTCA GTTCAAACAG TATATGCAGA ATCAGAATGT TCCGGGAAAT ATTGAGTTAA 
     3061 CTGTGAATAT CCTGACAATG GGCTATTGGC CGACATATGT GCCTATGGAA GTTCATTTAC 
     3121 CACCAGAGAT GGTAAAACTT CAGGAGATTT TCAAGACATT TTACCTAGGC AAACATAGTG 
     3181 GCAGGAAACT TCAGTGGCAG TCAACCATAG GACACTGTGT GTTAAAAGCA GAATTTAAAG 
     3241 AGGGTAAAAA GGAACTCCAG GTCTCTCTTT TTCAAACACT GGTGCTGCTA ATGTTTAATG 
     3301 AGGGAGAGGA GTTCAGTTTA GAAGAGATCA AGCAGGCAAC TGGAATAGAG GATGGAGAGT 
     3361 TAAGGAGAAC ACTGCAGTCA TTAGCCTGTG GCAAAGCTAG AGTTCTGGCG AAAAATCCAA 
     3421 AGGGCAAAGA CATTGAAGAT GGTGACAAGT TCATTTGTAA TGATGATTTC AAACATAAAC 
     3481 TTTTCAGGAT AAAGATCAAT CAAATCCAGA TGAAAGAAAC GGTTGAAGAA CAAGCAAGCA 
     3541 CTACAGAAAG AGTATTTCAA GACAGACAGT ATCAAATTGA TGCTGCAATT GTTCGAATTA 
     3601 TGAAGATGAG AAAGACACTT AGCCACAATC TCCTTGTTTC AGAAGTGTAC AACCAGTTGA 
     3661 AATTTCCAGT AAAGCCTGCT GATCTTAAGA AGAGAATAGA ATCTTTAATT GACCGGGACT 
     3721 ACATGGAAAG AGATAAAGAA AATCCAAACC AGTACAACTA TATTGCAACG CGTACGCGGC 
     3781 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3841 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3901 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3961 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4021 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4081 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4141 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4201 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4261 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4321 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4381 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4441 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4501 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4561 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4621 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5281 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5341 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5401 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5461 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5521 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5581 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5641 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5701 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5761 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5821 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5881 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5941 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6001 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6061 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6121 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6181 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6241 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6301 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6361 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6421 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6481 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6541 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6601 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6661 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6721 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6781 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6841 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6961 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7021 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7081 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7141 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7201 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7261 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7321 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7381 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7441 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7501 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7561 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
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