LOCUS       RC206822                8028 bp    DNA     circular  SYN 
DEFINITION  OGT (Myc-DDK-tagged)-Human O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) (OGT), transcript variant 1
ACCESSION   NM_181672
VERSION     NM_181672.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4183..4274 
                     /label="Myc-DDK"  
     misc_feature    4304..4889 
                     /label="PolyA signal"  
     misc_feature    4361..4380 
                     /label="Restriction Enzyme"  
     misc_feature    5165..5837 
                     /label="ColE1"  
     misc_feature    6267..7061 
                     /label="Kan/Neo"  
     misc_feature    7096..7398 
                     /label="SV40 ori"  
     misc_feature    7484..7961 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4158..4165 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4158 
                     /label="OGT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCTTCC GTGGGCAACG TGGCCGACAG CACAGAACCA ACGAAACGTA 
     1081 TGCTTTCCTT CCAAGGGTTA GCTGAGTTGG CACATCGAGA ATATCAGGCA GGAGATTTTG 
     1141 AGGCAGCTGA GAGACACTGC ATGCAGCTCT GGAGACAAGA GCCAGACAAT ACTGGTGTGC 
     1201 TTTTATTACT TTCATCTATA CACTTCCAGT GTCGAAGGCT GGACAGATCT GCTCACTTTA 
     1261 GCACTCTGGC AATTAAACAG AACCCCCTTC TGGCAGAAGC TTATTCGAAT TTGGGGAATG 
     1321 TGTACAAGGA AAGAGGGCAG TTGCAGGAGG CAATTGAGCA TTATCGACAT GCATTGCGTC 
     1381 TCAAACCTGA TTTCATCGAT GGTTATATTA ACCTGGCAGC CGCCTTGGTA GCAGCGGGTG 
     1441 ACATGGAAGG GGCAGTACAA GCTTACGTCT CTGCTCTTCA GTACAATCCT GATTTGTACT 
     1501 GTGTTCGCAG TGACCTGGGG AACCTGCTCA AAGCCCTGGG TCGCTTGGAA GAAGCCAAGG 
     1561 CATGTTATTT GAAAGCAATT GAGACGCAAC CGAACTTTGC AGTAGCTTGG AGTAATCTTG 
     1621 GCTGTGTTTT CAATGCACAA GGGGAAATTT GGCTTGCAAT TCATCACTTT GAAAAGGCTG 
     1681 TCACCCTTGA CCCAAACTTT CTGGATGCTT ATATCAATTT AGGAAATGTC TTGAAAGAGG 
     1741 CACGCATTTT TGACAGAGCT GTGGCAGCTT ATCTTCGTGC CCTAAGTTTG AGTCCAAATC 
     1801 ACGCAGTGGT GCACGGCAAC CTGGCTTGTG TATACTATGA GCAAGGCCTG ATAGATCTGG 
     1861 CAATAGACAC CTACAGGCGG GCTATCGAAC TACAACCACA TTTCCCTGAT GCTTACTGCA 
     1921 ACCTAGCCAA TGCTCTCAAA GAGAAGGGCA GTGTTGCTGA AGCAGAAGAT TGTTATAATA 
     1981 CAGCTCTCCG TCTGTGTCCC ACCCATGCAG ACTCTCTGAA TAACCTAGCC AATATCAAAC 
     2041 GAGAACAGGG AAACATTGAA GAGGCAGTTC GCTTGTATCG TAAAGCATTA GAAGTCTTCC 
     2101 CAGAGTTTGC TGCTGCCCAT TCAAATTTAG CAAGTGTACT GCAGCAGCAG GGAAAACTGC 
     2161 AGGAAGCTCT GATGCATTAT AAGGAGGCTA TTCGAATCAG TCCTACCTTT GCTGATGCCT 
     2221 ACTCTAATAT GGGAAACACT CTAAAGGAGA TGCAGGATGT TCAGGGAGCC TTGCAGTGTT 
     2281 ATACGCGTGC CATCCAAATT AATCCTGCAT TTGCAGATGC ACATAGCAAT CTGGCTTCCA 
     2341 TTCATAAGGA TTCAGGGAAT ATTCCAGAAG CCATAGCTTC TTACCGCACG GCTCTGAAAC 
     2401 TTAAGCCTGA TTTTCCTGAT GCTTATTGTA ACTTGGCTCA TTGCCTGCAG ATTGTCTGTG 
     2461 ATTGGACAGA CTATGATGAG CGAATGAAGA AGTTGGTCAG TATTGTGGCT GACCAGTTAG 
     2521 AGAAGAATAG GTTGCCTTCT GTGCATCCTC ATCATAGTAT GCTATATCCT CTTTCTCATG 
     2581 GCTTCAGGAA GGCTATTGCT GAGAGGCACG GCAACCTGTG CTTAGATAAG ATTAATGTTC 
     2641 TTCATAAACC ACCATATGAA CATCCAAAAG ACTTGAAGCT CAGTGATGGT CGGCTGCGTG 
     2701 TAGGATATGT GAGTTCCGAC TTTGGGAATC ATCCTACTTC TCACCTTATG CAGTCTATTC 
     2761 CAGGCATGCA CAATCCTGAT AAATTTGAGG TGTTCTGTTA TGCCCTGAGC CCAGACGATG 
     2821 GCACAAACTT CCGAGTGAAG GTGATGGCAG AAGCCAATCA TTTCATTGAT CTTTCTCAGA 
     2881 TTCCATGCAA TGGAAAAGCA GCTGATCGCA TCCATCAGGA TGGAATTCAT ATCCTTGTAA 
     2941 ATATGAATGG CTATACTAAG GGCGCTCGAA ATGAGCTTTT TGCTCTCAGG CCAGCTCCTA 
     3001 TTCAGGCAAT GTGGCTGGGA TACCCTGGGA CGAGTGGTGC GCTTTTCATG GATTATATTA 
     3061 TCACTGATCA GGAAACTTCG CCAGCTGAAG TTGCTGAGCA GTATTCCGAG AAATTGGCTT 
     3121 ATATGCCCCA CACTTTTTTT ATTGGTGATC ATGCTAATAT GTTCCCTCAC CTGAAGAAAA 
     3181 AAGCAGTCAT CGATTTTAAG TCCAATGGGC ACATTTATGA CAATCGGATA GTTCTGAATG 
     3241 GCATCGACCT CAAAGCATTT CTTGATAGTC TACCAGATGT GAAAATTGTC AAGATGAAGT 
     3301 GTCCTGATGG AGGAGACAAT GCAGATAGCA GTAACACAGC TCTTAATATG CCTGTTATTC 
     3361 CTATGAATAC TATTGCAGAA GCAGTTATTG AAATGATTAA CCGAGGACAG ATTCAAATAA 
     3421 CAATTAATGG ATTCAGTATT AGCAATGGAC TGGCAACTAC TCAGATCAAC AATAAGGCTG 
     3481 CAACTGGAGA GGAGGTTCCC CGTACCATTA TTGTAACCAC CCGTTCTCAG TACGGGTTAC 
     3541 CAGAAGATGC CATCGTATAC TGTAACTTTA ATCAGTTGTA TAAAATTGAC CCTTCTACTT 
     3601 TGCAGATGTG GGCAAACATT CTGAAGCGTG TTCCCAATAG TGTACTCTGG CTGTTGCGTT 
     3661 TTCCAGCAGT AGGAGAACCT AATATTCAAC AGTATGCACA AAACATGGGC CTGCCCCAGA 
     3721 ACCGTATCAT TTTTTCACCT GTTGCTCCTA AAGAGGAACA CGTCAGGAGA GGCCAGCTGG 
     3781 CTGATGTCTG CTTGGACACT CCACTCTGTA ATGGGCACAC CACAGGGATG GATGTCCTCT 
     3841 GGGCAGGGAC CCCCATGGTG ACTATGCCAG GAGAGACTCT TGCTTCTCGA GTTGCAGCAT 
     3901 CCCAGCTCAC TTGCTTAGGT TGTCTTGAGC TTATTGCTAA AAACAGACAA GAATATGAAG 
     3961 ACATAGCTGT GAAGCTGGGA ACTGATCTAG AATACCTGAA GAAAGTTCGT GGCAAAGTCT 
     4021 GGAAGCAAAG AATATCTAGC CCTCTGTTCA ACACCAAACA ATACACAATG GAACTAGAGC 
     4081 GGCTCTATCT ACAGATGTGG GAGCATTATG CAGCTGGCAA CAAACCTGAC CACATGATTA 
     4141 AGCCTGTTGA AGTCACTGAG TCAGCAAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     4201 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4261 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4321 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4381 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4441 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4501 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4561 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4621 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4681 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4741 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4801 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4861 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4921 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4981 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5041 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5101 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5161 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5221 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5281 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5341 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5401 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5461 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5521 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5581 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5641 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5701 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5761 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5821 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5881 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5941 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6001 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6061 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6121 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6181 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6241 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6301 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6361 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6421 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6481 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6541 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6601 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6661 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6721 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6781 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6841 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6901 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6961 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7021 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7081 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7141 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7201 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7261 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7441 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7501 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7561 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7621 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7681 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7741 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7801 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7861 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7921 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7981 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//