LOCUS       RC206740                7245 bp    DNA     circular  SYN 
DEFINITION  HID1 (Myc-DDK-tagged)-Human chromosome 17 open reading frame 28 (C17orf28)
ACCESSION   NM_030630
VERSION     NM_030630.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3402..3493 
                     /label="Myc-DDK"  
     misc_feature    3523..4108 
                     /label="PolyA signal"  
     misc_feature    3580..3599 
                     /label="Restriction Enzyme"  
     misc_feature    4384..5056 
                     /label="ColE1"  
     misc_feature    5486..6280 
                     /label="Kan/Neo"  
     misc_feature    6315..6617 
                     /label="SV40 ori"  
     misc_feature    6703..7180 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3384..3390 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3384 
                     /label="HID1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGTCGACC GACTCCAAGC TGAACTTCCG GAAGGCGGTG ATCCAGCTCA 
     1081 CCACCAAGAC GCAGCCCGTG GAAGCCACCG ATGATGCCTT TTGGGACCAG TTCTGGGCAG 
     1141 ACACAGCCAC CTCGGTGCAG GATGTGTTTG CACTGGTGCC GGCAGCAGAG ATCCGGGCCG 
     1201 TGCGGGAAGA GTCACCCTCC AACTTGGCCA CCCTGTGCTA CAAGGCCGTT GAGAAGCTGG 
     1261 TGCAGGGAGC TGAGAGTGGC TGCCACTCGG AGAAGGAGAA GCAGATCGTC CTGAACTGCA 
     1321 GCCGGCTGCT CACCCGCGTG CTGCCCTACA TCTTTGAGGA CCCCGACTGG AGGGGCTTCT 
     1381 TCTGGTCCAC AGTGCCCGGG GCAGGGCGAG GAGGGCAGGG AGAAGAGGAT GATGAGCATG 
     1441 CCAGGCCCCT GGCCGAGTCC CTGCTCCTGG CCATTGCTGA CCTGCTCTTC TGCCCGGACT 
     1501 TCACGGTTCA GAGCCACCGG AGGAGCACTG TGGACTCGGC AGAGGACGTC CACTCCCTGG 
     1561 ACAGCTGTGA ATACATCTGG GAGGCTGGTG TGGGCTTCGC TCACTCCCCC CAGCCTAACT 
     1621 ACATCCACGA TATGAACCGG ATGGAGCTGC TGAAACTGCT GCTGACATGC TTCTCCGAGG 
     1681 CCATGTACCT GCCCCCAGCT CCGGAAAGTG GCAGCACCAA CCCATGGGTT CAGTTCTTTT 
     1741 GTTCCACGGA GAACAGACAT GCCCTGCCCC TCTTCACCTC CCTCCTCAAC ACCGTGTGTG 
     1801 CCTATGACCC TGTGGGCTAC GGGATCCCCT ACAACCACCT GCTCTTCTCT GACTACCGGG 
     1861 AACCCCTGGT GGAGGAGGCT GCCCAGGTGC TCATTGTCAC TTTGGACCAC GACAGTGCCA 
     1921 GCAGTGCCAG CCCCACTGTG GACGGCACCA CCACTGGCAC CGCCATGGAT GATGCCGATC 
     1981 CTCCAGGCCC TGAGAACCTG TTTGTGAACT ACCTGTCCCG CATCCATCGT GAGGAGGACT 
     2041 TCCAGTTCAT CCTCAAGGGT ATAGCCCGGC TGCTGTCCAA CCCCCTGCTC CAGACCTACC 
     2101 TGCCTAACTC CACCAAGAAG ATCCAGTTCC ACCAGGAGCT GCTAGTTCTC TTCTGGAAGC 
     2161 TCTGCGACTT CAACAAGAAA TTCCTCTTCT TCGTGCTGAA GAGCAGCGAC GTCCTAGACA 
     2221 TCCTTGTCCC CATCCTCTTC TTCCTCAACG ATGCCCGGGC CGATCAGTCT CGGGTGGGCC 
     2281 TGATGCACAT TGGTGTCTTC ATCTTGCTGC TTCTGAGCGG GGAGCGGAAC TTCGGGGTGC 
     2341 GGCTGAACAA ACCCTACTCA ATCCGCGTGC CCATGGACAT CCCAGTCTTC ACAGGGACCC 
     2401 ACGCCGACCT GCTCATTGTG GTGTTCCACA AGATCATCAC CAGCGGGCAC CAGCGGTTGC 
     2461 AGCCCCTCTT CGACTGCCTG CTCACCATCG TGGTCAACGT GTCCCCCTAC CTCAAGAGCC 
     2521 TGTCCATGGT GACCGCCAAC AAGTTGCTGC ACCTGCTGGA GGCCTTCTCC ACCACCTGGT 
     2581 TCCTCTTCTC TGCCGCCCAG AACCACCACC TGGTCTTCTT CCTCCTGGAG GTCTTCAACA 
     2641 ACATCATCCA GTACCAGTTT GATGGCAACT CCAACCTGGT CTACGCCATC ATCCGCAAGC 
     2701 GCAGCATCTT CCACCAGCTG GCCAACCTGC CCACGGACCC GCCCACCATT CACAAGGCCC 
     2761 TGCAGCGGCG CCGGCGGACA CCTGAGCCCT TGTCTCGCAC CGGCTCCCAG GAGGGCACCT 
     2821 CCATGGAGGG CTCCCGCCCC GCTGCCCCTG CAGAGCCAGG CACCCTCAAG ACCAGTCTGG 
     2881 TGGCTACTCC AGGCATTGAC AAGCTGACCG AGAAGTCCCA GGTGTCAGAG GATGGCACCT 
     2941 TGCGGTCCCT GGAACCTGAG CCCCAGCAGA GCTTGGAGGA TGGCAGCCCG GCTAAGGGGG 
     3001 AGCCCAGCCA GGCATGGAGG GAGCAGCGGC GACCGTCCAC CTCATCAGCC AGTGGGCAGT 
     3061 GGAGCCCAAC GCCAGAGTGG GTCCTCTCCT GGAAGTCGAA GCTGCCGCTG CAGACCATCA 
     3121 TGAGGCTGCT GCAGGTGCTG GTTCCGCAGG TGGAGAAGAT CTGCATCGAC AAGGGCCTGA 
     3181 CGGATGAGTC TGAGATCCTG CGGTTCCTGC AGCATGGCAC CCTGGTGGGG CTGCTGCCCG 
     3241 TGCCCCACCC CATCCTCATC CGCAAGTACC AGGCCAACTC GGGCACTGCC ATGTGGTTCC 
     3301 GCACCTACAT GTGGGGCGTC ATCTATCTGA GGAATGTGGA CCCCCCTGTC TGGTACGACA 
     3361 CCGACGTGAA GCTGTTTGAG ATACAGCGGG TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3421 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3481 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3541 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3601 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3661 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     3721 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     3781 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     3841 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     3901 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     3961 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4021 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4081 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4141 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4201 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4261 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4321 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4381 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4441 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4501 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4561 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4621 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4681 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     4741 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     4801 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     4861 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     4921 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     4981 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5041 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5101 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5161 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5221 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5281 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5341 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5401 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5461 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5521 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5581 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5641 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     5701 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     5761 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     5821 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     5881 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     5941 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6001 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6061 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6121 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6181 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6241 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6301 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6361 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6421 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6481 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6541 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6601 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6661 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     6721 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     6781 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     6841 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     6901 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     6961 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7021 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7081 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7141 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7201 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//