LOCUS       RC206622                6708 bp    DNA     circular  SYN 
DEFINITION  AFP (Myc-DDK-tagged)-Human alpha-fetoprotein (AFP)
ACCESSION   NM_001134
VERSION     NM_001134.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2865..2956 
                     /label="Myc-DDK"  
     misc_feature    2986..3571 
                     /label="PolyA signal"  
     misc_feature    3043..3062 
                     /label="Restriction Enzyme"  
     misc_feature    3847..4519 
                     /label="ColE1"  
     misc_feature    4949..5743 
                     /label="Kan/Neo"  
     misc_feature    5778..6080 
                     /label="SV40 ori"  
     misc_feature    6166..6643 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2847..2853 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2847 
                     /label="AFP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTGGGTG GAATCAATTT TTTTAATTTT CCTACTAAAT TTTACTGAAT 
     1081 CCAGAACACT GCATAGAAAT GAATATGGAA TAGCTTCCAT ATTGGATTCT TACCAATGTA 
     1141 CTGCAGAGAT AAGTTTAGCT GACCTGGCTA CCATATTTTT TGCCCAGTTT GTTCAAGAAG 
     1201 CCACTTACAA GGAAGTAAGC AAAATGGTGA AAGATGCATT GACTGCAATT GAGAAACCCA 
     1261 CTGGAGATGA ACAGTCTTCA GGGTGTTTAG AAAACCAGCT ACCTGCCTTT CTGGAAGAAC 
     1321 TTTGCCATGA GAAAGAAATT TTGGAGAAGT ACGGACATTC AGACTGCTGC AGCCAAAGTG 
     1381 AAGAGGGAAG ACATAACTGT TTTCTTGCAC ACAAAAAGCC CACTCCAGCA TCGATCCCAC 
     1441 TTTTCCAAGT TCCAGAACCT GTCACAAGCT GTGAAGCATA TGAAGAAGAC AGGGAGACAT 
     1501 TCATGAACAA ATTCATTTAT GAGATAGCAA GAAGGCATCC CTTCCTGTAT GCACCTACAA 
     1561 TTCTTCTTTG GGCTGCTCGC TATGACAAAA TAATTCCATC TTGCTGCAAA GCTGAAAATG 
     1621 CAGTTGAATG CTTCCAAACA AAGGCAGCAA CAGTTACAAA AGAATTAAGA GAAAGCAGCT 
     1681 TGTTAAATCA ACATGCATGT GCAGTAATGA AAAATTTTGG GACCCGAACT TTCCAAGCCA 
     1741 TAACTGTTAC TAAACTGAGT CAGAAGTTTA CCAAAGTTAA TTTTACTGAA ATCCAGAAAC 
     1801 TAGTCCTGGA TGTGGCCCAT GTACATGAGC ACTGTTGCAG AGGAGATGTG CTGGATTGTC 
     1861 TGCAGGATGG GGAAAAAATC ATGTCCTACA TATGTTCTCA ACAAGACACT CTGTCAAACA 
     1921 AAATAACAGA ATGCTGCAAA CTGACCACGC TGGAACGTGG TCAATGTATA ATTCATGCAG 
     1981 AAAATGATGA AAAACCTGAA GGTCTATCTC CAAATCTAAA CAGGTTTTTA GGAGATAGAG 
     2041 ATTTTAACCA ATTTTCTTCA GGGGAAAAAA ATATCTTCTT GGCAAGTTTT GTTCATGAAT 
     2101 ATTCAAGAAG ACATCCTCAG CTTGCTGTCT CAGTAATTCT AAGAGTTGCT AAAGGATACC 
     2161 AGGAGTTATT GGAGAAGTGT TTCCAGACTG AAAACCCTCT TGAATGCCAA GATAAAGGAG 
     2221 AAGAAGAATT ACAGAAATAC ATCCAGGAGA GCCAAGCATT GGCAAAGCGA AGCTGCGGCC 
     2281 TCTTCCAGAA ACTAGGAGAA TATTACTTAC AAAATGCGTT TCTCGTTGCT TACACAAAGA 
     2341 AAGCCCCCCA GCTGACCTCG TCGGAGCTGA TGGCCATCAC CAGAAAAATG GCAGCCACAG 
     2401 CAGCCACTTG TTGCCAACTC AGTGAGGACA AACTATTGGC CTGTGGCGAG GGAGCGGCTG 
     2461 ACATTATTAT CGGACACTTA TGTATCAGAC ATGAAATGAC TCCAGTAAAC CCTGGTGTTG 
     2521 GCCAGTGCTG CACTTCTTCA TATGCCAACA GGAGGCCATG CTTCAGCAGC TTGGTGGTGG 
     2581 ATGAAACATA TGTCCCTCCT GCATTCTCTG ATGACAAGTT CATTTTCCAT AAGGATCTGT 
     2641 GCCAAGCTCA GGGTGTAGCG CTGCAAACGA TGAAGCAAGA GTTTCTCATT AACCTTGTGA 
     2701 AGCAAAAGCC ACAAATAACA GAGGAACAAC TTGAGGCTGT CATTGCAGAT TTCTCAGGCC 
     2761 TGTTGGAGAA ATGCTGCCAA GGCCAGGAAC AGGAAGTCTG CTTTGCTGAA GAGGGACAAA 
     2821 AACTGATTTC AAAAACTCGT GCTGCTTTGG GAGTTACGCG TACGCGGCCG CTCGAGCAGA 
     2881 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     2941 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3001 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3061 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3121 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3181 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3241 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3301 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3361 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3421 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3481 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3541 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     3601 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     3661 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     3721 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     3781 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     3841 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     3901 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     3961 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4021 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4081 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4141 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4201 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4261 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4321 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4381 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4441 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4501 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4561 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     4621 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     4681 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     4741 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     4801 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     4861 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     4921 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     4981 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5041 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5101 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5161 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5221 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5281 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5341 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5401 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5461 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5521 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     5581 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     5641 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     5701 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     5761 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     5821 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     5881 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     5941 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6001 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6061 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6121 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6181 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6241 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6301 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6361 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6421 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6481 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6541 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     6601 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     6661 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//