LOCUS       RC206486                8346 bp    DNA     circular  SYN 
DEFINITION  DHX30 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 2
ACCESSION   NM_014966
VERSION     NM_014966.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4503..4594 
                     /label="Myc-DDK"  
     misc_feature    4624..5209 
                     /label="PolyA signal"  
     misc_feature    4681..4700 
                     /label="Restriction Enzyme"  
     misc_feature    5485..6157 
                     /label="ColE1"  
     misc_feature    6587..7381 
                     /label="Kan/Neo"  
     misc_feature    7416..7718 
                     /label="SV40 ori"  
     misc_feature    7804..8281 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4485..4491 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4485 
                     /label="DHX30"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCTTCT AGGGACCTAT TAAAAGAGTT CCCACAGCCC AAAAATCTTC 
     1081 TCAACAGTGT GATTGGAAGA GCCCTCGGCA TCTCACATGC AAAAGACAAA CTAGTCTACG 
     1141 TGCACACAAA TGGACCGAAG AAAAAGAAAG TCACACTGCA CATAAAATGG CCCAAGAGCG 
     1201 TGGAGGTAGA AGGCTATGGC AGCAAGAAGA TCGATGCTGA GCGGCAGGCT GCAGCTGCAG 
     1261 CCTGCCAGCT GTTCAAGGGT TGGGGTCTGC TAGGTCCCCG GAATGAGTTG TTTGACGCAG 
     1321 CCAAATACCG AGTGCTAGCT GATCGCTTTG GCTCCCCTGC CGACAGCTGG TGGCGTCCGG 
     1381 AACCCACCAT GCCCCCTACT TCCTGGCGGC AGCTGAATCC AGAGAGTATT CGACCAGGGG 
     1441 GACCTGGGGG CCTATCCCGC TCTTTAGGCC GGGAAGAAGA GGAGGACGAG GAGGAAGAGC 
     1501 TAGAAGAAGG GACCATAGAT GTTACCGACT TCTTGTCCAT GACCCAGCAG GATTCCCACG 
     1561 CTCCACTCAG GGACTCAAGG GGGAGTTCCT TTGAGATGAC AGATGACGAC AGTGCCATTA 
     1621 GGGCTCTGAC CCAGTTTCCA CTTCCCAAGA ACCTTCTGGC CAAGGTGATT CAGATTGCAA 
     1681 CGTCATCCTC CACAGCTAAG AACCTCATGC AGTTCCATAC TGTGGGCACC AAGACCAAGC 
     1741 TGTCTACACT CACCCTGCTC TGGCCCTGCC CCATGACCTT TGTTGCCAAA GGGCGCCGCA 
     1801 AAGCAGAGGC TGAGAATAAG GCGGCAGCCT TGGCCTGCAA GAAACTGAAG AGCCTGGGCC 
     1861 TGGTGGACAG GAACAACGAA CCGCTTACAC ACGCCATGTA TAACCTGGCC TCTTTGCGTG 
     1921 AGCTGGGTGA GACCCAGCGC CGACCATGCA CCATCCAGGT GCCCGAGCCC ATCCTCCGCA 
     1981 AGATAGAGAC CTTCCTGAAC CATTACCCTG TGGAGAGTTC ATGGATCGCC CCAGAACTCC 
     2041 GGCTGCAGAG TGATGACATC TTGCCCTTGG GCAAGGACTC AGGGCCTCTG AGTGACCCTA 
     2101 TCACAGGCAA GCCCTATGTG CCCCTGTTGG AAGCAGAGGA GGTACGTCTC AGCCAGAGTC 
     2161 TGCTAGAACT GTGGCGGCGG CGAGGGCCGG TCTGGCAGGA GGCCCCCCAG CTACCTGTGG 
     2221 ACCCACATCG GGACACCATC CTCAACGCCA TTGAGCAGCA CCCGGTGGTG GTCATCTCTG 
     2281 GGGACACGGG CTGTGGGAAG ACCACGCGCA TCCCCCAGCT GTTGCTGGAG CGCTATGTGA 
     2341 CCGAGGGCCG AGGTGCCCGC TGCAATGTTA TCATCACCCA ACCTCGCCGC ATCTCTGCTG 
     2401 TGTCTGTGGC ACAGCGGGTC AGCCACGAAC TGGGCCCCTC CCTGCGCCGG AATGTGGGCT 
     2461 TCCAGGTGCG GTTGGAAAGT AAGCCCCCAT CCCGAGGCGG GGCCCTGCTC TTCTGCACTG 
     2521 TGGGTATCCT GCTGCGTAAG CTGCAGAGCA ACCCCAGCCT GGAGGGCGTG AGCCACGTCA 
     2581 TCGTGGATGA GGTGCATGAG CGGGACGTGA ACACAGACTT TCTGCTGATC CTGCTCAAGG 
     2641 GCCTGCAGCG GCTCAACCCG GCCCTGCGGC TGGTGCTCAT GAGTGCCACA GGGGACAATG 
     2701 AGCGCTTCTC CCGATACTTT GGTGGCTGCC CCGTCATCAA GGTGCCTGGC TTCATGTACC 
     2761 CAGTCAAGGA GCACTACCTA GAGGACATCC TGGCCAAGTT GGGCAAGCAC CAGTACCTGC 
     2821 ACCGGCACCG GCACCATGAG TCTGAGGATG AATGCGCACT CGATTTGGAC CTTGTGACTG 
     2881 ATCTGGTTCT GCACATCGAT GCTCGCGGGG AACCAGGTGG GATCCTGTGC TTCCTGCCTG 
     2941 GGTGGCAGGA GATCAAAGGA GTGCAGCAGC GCCTCCAGGA GGCCCTGGGC ATGCACGAGA 
     3001 GCAAGTACCT CATCCTGCCA GTGCACTCCA ACATCCCCAT GATGGATCAG AAGGCCATAT 
     3061 TCCAGCAGCC TCCAGTTGGG GTGCGCAAGA TTGTCTTGGC CACCAACATT GCTGAGACTT 
     3121 CCATCACAAT CAATGACATC GTGCATGTGG TGGACAGTGG GCTGCACAAG GAAGAACGCT 
     3181 ATGACCTGAA GACCAAGGTG TCCTGCCTGG AGACAGTGTG GGTATCAAGA GCCAATGTGA 
     3241 TCCAGCGCCG GGGCCGGGCG GGCCGCTGCC AGTCCGGCTT TGCCTACCAC TTGTTCCCTC 
     3301 GAAGCCGGCT GGAGAAAATG GTCCCTTTCC AAGTGCCAGA GATCCTGCGC ACACCTCTTG 
     3361 AGAACCTGGT GCTGCAAGCG AAAATCCACA TGCCTGAGAA GACGGCGGTG GAGTTCCTGT 
     3421 CCAAGGCTGT GGACAGTCCA AACATCAAGG CAGTGGACGA GGCTGTGATC TTGCTCCAGG 
     3481 AGATCGGGGT GCTGGACCAG CGGGAGTACC TGACTACCCT GGGGCAGCGC CTGGCTCACA 
     3541 TCTCCACCGA CCCCCGGTTG GCCAAGGCCA TTGTGTTGGC TGCCATCTTC CGTTGCCTGC 
     3601 ACCCACTACT GGTGGTCGTT TCCTGCCTCA CCCGGGACCC CTTCAGCAGC AGCCTACAGA 
     3661 ACCGGGCAGA GGTGGACAAG GTGAAAGCAC TGTTGAGCCA TGACAGCGGC AGTGACCACC 
     3721 TGGCCTTTGT GCGGGCTGTC GCCGGCTGGG AGGAGGTGCT GCGTTGGCAG GACCGCAGCT 
     3781 CCCGGGAGAA TTACCTGGAG GAAAACCTGC TGTACGCACC CAGCCTGCGC TTCATCCACG 
     3841 GACTCATCAA GCAGTTCTCA GAGAACATTT ATGAGGCCTT CCTGGTGGGG AAGCCCTCGG 
     3901 ACTGCACCCT GGCCTCCGCC CAGTGCAACG AGTACAGTGA GGAGGAGGAG CTGGTGAAGG 
     3961 GCGTGCTGAT GGCCGGCCTC TACCCCAACC TCATCCAGGT GAGGCAGGGC AAGGTCACCC 
     4021 GGCAGGGGAA GTTCAAGCCC AACAGCGTCA CATATAGGAC CAAATCAGGC AACATCCTGC 
     4081 TGCACAAGTC GACCATTAAC AGGGAGGCCA CACGGTTACG GAGCCGATGG CTGACGTATT 
     4141 TCATGGCAGT CAAGTCCAAT GGCAGCGTCT TCGTCCGGGA CTCCTCTCAG GTGCACCCGC 
     4201 TAGCTGTGCT GCTGCTGACC GACGGGGACG TGCACATCCG TGATGACGGG CGCCGGGCCA 
     4261 CCATCTCACT GAGCGACAGT GACCTGCTGC GGCTGGAGGG TGACTCGCGT ACCGTGCGGC 
     4321 TGCTGAAGGA GCTGCGGCGG GCCCTGGGCC GCATGGTGGA GCGGAGCCTG CGCAGCGAGC 
     4381 TGGCTGCACT TCCCCCCAGC GTACAGGAGG AGCACGGGCA GCTGCTTGCG CTACTGGCAG 
     4441 AGCTGCTGCG AGGACCCTGT GGCAGCTTTG ATGTGCGCAA GACAGCTGAC GACACGCGTA 
     4501 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4561 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4621 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4681 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4741 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4801 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4861 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4921 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4981 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5041 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5101 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5161 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5221 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5281 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5341 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5401 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5461 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5521 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5581 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5641 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5701 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5761 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5821 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5881 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5941 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6001 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6061 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6121 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6181 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6241 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6301 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6361 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6421 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6481 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6541 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6601 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6661 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6721 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6781 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6841 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6901 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6961 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7021 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7081 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7141 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7201 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7261 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7321 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7381 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7441 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7501 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7561 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7801 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7861 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7921 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7981 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8041 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8101 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8161 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8221 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8281 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8341 AATTTT
//