LOCUS       RC206464L1                8571 bp    DNA     circular  SYN 
DEFINITION  F13A1 (Myc-DDK-tagged)-Human coagulation factor XIII, A1 polypeptide (F13A1)
ACCESSION   NM_000129
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6862..7042 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7770..8429 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7201..7769 
                     /label="ColE1"  
     misc_feature    5737..5757 
                     /label="LR50 primer"  
     misc_feature    5534..5605 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5746..6337 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5516..5522 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5516 
                     /label="F13A1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTCAGAAACT TCCAGGACCG CCTTTGGAGG 
     3361 CAGAAGAGCA GTTCCACCCA ATAACTCTAA TGCAGCGGAA GATGACCTGC CCACAGTGGA 
     3421 GCTTCAGGGC GTGGTGCCCC GGGGCGTCAA CCTGCAAGAG TTTCTTAATG TCACGAGCGT 
     3481 TCACCTGTTC AAGGAGAGAT GGGACACTAA CAAGGTGGAC CACCACACTG ACAAGTATGA 
     3541 AAACAACAAG CTGATTGTCC GCAGAGGGCA GTCTTTCTAT GTGCAGATTG ACTTCAGTCG 
     3601 TCCATATGAC CCCAGAAGGG ATCTCTTCAG GGTGGAATAC GTCATTGGTC GCTACCCACA 
     3661 GGAGAACAAG GGAACCTACA TCCCAGTGCC TATAGTCTCA GAGTTACAAA GTGGAAAGTG 
     3721 GGGGGCCAAG ATTGTCATGA GAGAGGACAG GTCTGTGCGG CTGTCCATCC AGTCTTCCCC 
     3781 CAAATGTATT GTGGGGAAAT TCCGCATGTA TGTTGCTGTC TGGACTCCCT ATGGCGTACT 
     3841 TCGAACCAGT CGAAACCCAG AAACAGACAC GTACATTCTC TTCAATCCTT GGTGTGAAGA 
     3901 TGATGCTGTG TATCTGGACA ATGAGAAAGA AAGAGAAGAG TATGTCCTGA ATGACATCGG 
     3961 GGTAATTTTT TATGGAGAGG TCAATGACAT CAAGACCAGA AGCTGGAGCT ATGGTCAGTT 
     4021 TGAAGATGGC ATCCTGGACA CTTGCCTGTA TGTGATGGAC AGAGCACAAA TGGACCTCTC 
     4081 TGGAAGAGGG AATCCCATCA AAGTCAGCCG TGTGGGGTCT GCAATGGTGA ATGCCAAAGA 
     4141 TGACGAAGGT GTCCTCGTTG GATCCTGGGA CAATATCTAT GCCTATGGCG TCCCCCCATC 
     4201 GGCCTGGACT GGAAGCGTTG ACATTCTATT GGAATACCGG AGCTCTGAGA ATCCAGTCCG 
     4261 GTATGGCCAA TGCTGGGTTT TTGCTGGTGT CTTTAACACA TTTTTACGAT GCCTTGGAAT 
     4321 ACCAGCAAGA ATTGTTACCA ATTATTTCTC TGCCCATGAT AATGATGCCA ATTTGCAAAT 
     4381 GGACATCTTC CTGGAAGAAG ATGGGAACGT GAATTCCAAA CTCACCAAGG ATTCAGTGTG 
     4441 GAACTACCAC TGCTGGAATG AAGCATGGAT GACAAGGCCT GACCTTCCTG TTGGATTTGG 
     4501 AGGCTGGCAA GCTGTGGACA GCACCCCCCA GGAAAATAGC GATGGCATGT ATCGGTGTGG 
     4561 CCCCGCCTCG GTTCAAGCCA TCAAGCACGG CCATGTCTGC TTCCAATTTG ATGCACCTTT 
     4621 TGTTTTTGCA GAGGTCAACA GCGACCTCAT TTACATTACA GCTAAGAAAG ATGGCACTCA 
     4681 TGTGGTGGAA AATGTGGATG CCACCCACAT TGGGAAATTA ATTGTGACCA AACAAATTGG 
     4741 AGGAGATGGC ATGATGGATA TTACTGATAC TTACAAATTC CAAGAAGGTC AAGAAGAAGA 
     4801 GAGATTGGCC CTAGAAACTG CCCTGATGTA CGGAGCTAAA AAGCCCCTCA ACACAGAAGG 
     4861 TGTCATGAAA TCAAGGTCCA ACGTTGACAT GGACTTTGAA GTGGAAAATG CTGTGCTGGG 
     4921 AAAAGACTTC AAGCTCTCCA TCACCTTCCG GAACAACAGC CACAACCGTT ACACCATCAC 
     4981 AGCTTATCTC TCAGCCAACA TCACCTTCTA CACCGGGGTC CCGAAGGCAG AATTCAAGAA 
     5041 GGAGACGTTC GACGTGACGC TGGAGCCCTT GTCCTTCAAG AAAGAGGCGG TGCTGATCCA 
     5101 AGCCGGCGAG TACATGGGTC AGCTGCTGGA ACAAGCGTCC CTGCACTTCT TTGTCACAGC 
     5161 TCGCATCAAT GAGACCAGGG ATGTTCTGGC CAAGCAAAAG TCCACCGTGC TAACCATCCC 
     5221 TGAGATCATC ATCAAGGTCC GTGGCACTCA GGTAGTTGGT TCTGACATGA CTGTGATAGT 
     5281 TGAGTTTACC AATCCTTTAA AAGAAACCCT GCGAAATGTC TGGGTACACC TGGATGGTCC 
     5341 TGGAGTAACA AGACCAATGA AGAAGATGTT CCGTGAAATC CGGCCCAACT CCACCGTGCA 
     5401 GTGGGAAGAA GTGTGCCGGC CCTGGGTCTC TGGGCATCGG AAGCTGATAG CCAGCATGAG 
     5461 CAGTGACTCC CTGAGACATG TGTATGGCGA GCTGGACGTG CAGATTCAAA GACGACCTTC 
     5521 CATGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     5581 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     5641 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     5701 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     5761 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     5821 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     5881 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     5941 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6001 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     6061 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     6121 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     6181 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     6241 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     6301 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     6361 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     6421 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     6481 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     6541 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     6601 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     6661 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     6721 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     6781 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     6841 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     6901 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     6961 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7021 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     7081 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     7141 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     7201 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     7261 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     7321 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     7381 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     7441 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     7501 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     7561 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     7621 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     7681 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     7741 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     7801 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     7861 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     7921 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     7981 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     8041 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     8101 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     8161 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     8221 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     8281 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     8341 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     8401 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     8461 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8521 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//