LOCUS RC206423 6453 bp DNA circular SYN DEFINITION CYP4F12 (Myc-DDK-tagged)-Human cytochrome P450, family 4, subfamily F, polypeptide 12 (CYP4F12) ACCESSION NM_023944 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2610..2701 /label="Myc-DDK" misc_feature 2731..3316 /label="PolyA signal" misc_feature 2788..2807 /label="Restriction Enzyme" misc_feature 3592..4264 /label="ColE1" misc_feature 4694..5488 /label="Kan/Neo" misc_feature 5523..5825 /label="SV40 ori" misc_feature 5911..6388 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2592..2598 /label="Restriction Enzyme" misc_feature 1020..2592 /label="RC206423" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GTCGCTGCTG AGCCTGCCCT GGCTGGGCCT CAGACCGGTG GCAATGTCCC 1081 CATGGCTACT CCTGCTGCTG GTTGTGGGCT CCTGGCTACT CGCCCGCATC CTGGCTTGGA 1141 CCTATGCCTT CTATAACAAC TGCCGCCGGC TCCAGTGTTT CCCACAGCCC CCAAAACGGA 1201 ACTGGTTTTG GGGTCACCTG GGCCTGATCA CTCCTACAGA GGAGGGCTTG AAGGACTCGA 1261 CCCAGATGTC GGCCACCTAT TCCCAGGGCT TTACGGTATG GCTGGGTCCC ATCATCCCCT 1321 TCATCGTTTT ATGCCACCCT GACACCATCC GGTCTATCAC CAATGCCTCA GCTGCCATTG 1381 CACCCAAGGA TAATCTCTTC ATCAGGTTCC TGAAGCCCTG GCTGGGAGAA GGGATACTGC 1441 TGAGTGGCGG TGACAAGTGG AGCCGCCACC GTCGGATGCT GACGCCCGCC TTCCATTTCA 1501 ACATCCTGAA GTCCTATATA ACGATCTTCA ACAAGAGTGC AAACATCATG CTTGACAAGT 1561 GGCAGCACCT GGCCTCAGAG GGCAGCAGTT GTCTGGACAT GTTTGAGCAC ATCAGCCTCA 1621 TGACCTTGGA CAGTCTACAG AAATGCATCT TCAGCTTTGA CAGCCATTGT CAGGAGAGGC 1681 CCAGTGAATA TATTGCCACC ATCTTGGAGC TCAGTGCCCT TGTAGAGAAA AGAAGCCAGC 1741 ATATCCTCCA GCACATGGAC TTTCTGTATT ACCTCTCCCA TGACGGGCGG CGCTTCCACA 1801 GGGCCTGCCG CCTGGTGCAT GACTTCACAG ACGCTGTCAT CCGGGAGCGG CGTCGCACCC 1861 TCCCCACTCA GGGTATTGAT GATTTTTTCA AAGACAAAGC CAAGTCCAAG ACTTTGGATT 1921 TCATTGATGT GCTTCTGCTG AGCAAGGATG AAGATGGGAA GGCATTGTCA GATGAGGATA 1981 TAAGAGCAGA GGCTGACACC TTCATGTTTG GAGGCCATGA CACCACGGCC AGTGGCCTCT 2041 CCTGGGTCCT GTACAACCTT GCGAGGCACC CAGAATACCA GGAGCGCTGC CGACAGGAGG 2101 TGCAAGAGCT TCTGAAGGAC CGCGATCCTA AAGAGATTGA ATGGGACGAC CTGGCCCAGC 2161 TGCCCTTCCT GACCATGTGC GTGAAGGAGA GCCTGAGGTT ACATCCCCCA GCTCCCTTCA 2221 TCTCCCGATG CTGCACCCAG GACATTGTTC TCCCAGATGG CCGAGTCATC CCCAAAGGCA 2281 TTACCTGCCT CATCGATATT ATAGGGGTCC ATCACAACCC AACTGTGTGG CCGGATCCTG 2341 AGGTCTACGA CCCCTTCCGC TTTGACCCAG AGAACAGCAA GGGGAGGTCA CCTCTGGCTT 2401 TTATTCCTTT CTCCGCAGGG CCCAGGAACT GCATCGGGCA GGCGTTCGCC ATGGCGGAGA 2461 TGAAAGTGGT CCTGGCGTTG ATGCTGCTGC ACTTCCGGTT CCTGCCAGAC CACACTGAGC 2521 CCCGCAGGAA GCTGGAATTG ATCATGCGCG CCGAGGGCGG GCTTTGGCTG CGGGTGGAGC 2581 CCCTGAATGT AAGCTTGCAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 2641 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 2701 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 2761 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 2821 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 2881 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 2941 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 3001 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 3061 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 3121 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 3181 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 3241 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 3301 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 3361 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 3421 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 3481 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 3541 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 3601 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 3661 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 3721 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 3781 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 3841 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 3901 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 3961 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 4021 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 4081 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 4141 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 4201 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 4261 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 4321 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 4381 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 4441 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 4501 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 4561 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 4621 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 4681 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 4741 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 4801 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 4861 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 4921 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 4981 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 5041 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 5101 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 5161 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 5221 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 5281 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 5341 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 5401 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 5461 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 5521 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 5581 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 5641 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 5701 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 5761 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 5821 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 5881 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 5941 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 6001 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 6061 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 6121 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 6181 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 6241 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 6301 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 6361 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 6421 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT //