LOCUS       RC206255                8505 bp    DNA     circular  SYN 
DEFINITION  AMBRA1 (Myc-DDK-tagged)-Human autophagy/beclin-1 regulator 1 (AMBRA1)
ACCESSION   NM_017749
VERSION     NM_017749.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4662..4753 
                     /label="Myc-DDK"  
     misc_feature    4783..5368 
                     /label="PolyA signal"  
     misc_feature    4840..4859 
                     /label="Restriction Enzyme"  
     misc_feature    5644..6316 
                     /label="ColE1"  
     misc_feature    6746..7540 
                     /label="Kan/Neo"  
     misc_feature    7575..7877 
                     /label="SV40 ori"  
     misc_feature    7963..8440 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4644..4650 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4644 
                     /label="AMBRA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTTGTC CCAGAAAAGA ATGCTGTCCG GATACTCTGG GGGCGAGAAC 
     1081 GGGGTGCTCG GGCCATGGGA GCTCAGCGGC TTCTGCAGGA GCTGGTAGAG GATAAAACCC 
     1141 GGTGGATGAA ATGGGAGGGC AAGAGAGTAG AACTGCCGGA TAGTCCACGC TCTACCTTCT 
     1201 TATTGGCCTT CAGCCCAGAC AGGACTCTCT TAGCCTCCAC CCATGTGAAC CATAATATCT 
     1261 ATATTACGGA GGTGAAGACT GGCAAGTGTG TTCATTCCCT GATTGGACAC CGCCGTACTC 
     1321 CATGGTGTGT CACTTTTCAT CCCACCATCT CAGGCCTTAT TGCTTCTGGC TGCCTAGATG 
     1381 GGGAGGTTAG GATTTGGGAT TTACACGGTG GCAGTGAAAG CTGGTTCACA GATAGCAACA 
     1441 ATGCCATTGC CTCCCTGGCT TTCCACCCTA CGGCTCAGCT CCTGCTGATT GCCACTGCCA 
     1501 ATGAGATCCA CTTCTGGGAC TGGAGTCGAC GGGAACCCTT TGCTGTGGTG AAGACAGCTA 
     1561 GTGAGATGGA ACGGGTCCGT CTGGTGAGAT TTGATCCACT TGGACACTAC TTACTCACAG 
     1621 CAATTGTTAA CCCCTCTAAT CAACAGGGTG ATGACGAACC AGAGATCCCC ATAGATGGAA 
     1681 CAGAATTATC CCACTACCGT CAGCGTGCCC TCCTGCAATC ACAGCCAGTT CGCCGGACGC 
     1741 CTCTCCTCCA CAATTTCCTG CACATGCTGT CCTCCCGCTC TTCTGGCATC CAGACCGAGC 
     1801 CCTTCCATCC CCCGGAGCAG GCCTCGTCAA CGCAGCAGGA CCAGGGCCTC CTGAACCGGC 
     1861 CGTCTGCCTT CAGTACAGTC CAGAGCAGCA CTGCCGGCAA CACGCTCCGC AACCTCAGTC 
     1921 TGGGTCCTAC CCGCCGCTCT TTGGGAGGGC CTCTGTCTAG CCACCCTTCT AGGTATCACC 
     1981 GAGAAATAGC TCCTGGGTTG ACAGGATCTG AGTGGACCCG GACAGTACTC AGTCTGAACT 
     2041 CCCGCTCTGA GGCGGAATCC ATGCCCCCGC CCAGAACCAG TGCCTCTTCG GTGAGTTTGC 
     2101 TGTCTGTGCT GAGACAGCAG GAAGGTGGCT CTCAGGCATC TGTGTACACT TCAGCCACAG 
     2161 AAGGGAGGGG TTTTCCGGCA TCAGGGTTGG CAACTGAGTC AGATGGAGGG AATGGCTCCA 
     2221 GCCAAAACAA CTCGGGCAGC ATTCGCCATG AGCTTCAGTG TGACCTGAGA CGCTTCTTTC 
     2281 TGGAGTATGA CCGGCTTCAG GAGCTGGATC AGAGCCTGAG TGGGGAAGCT CCCCAGACCC 
     2341 AACAGGCCCA GGAAATGCTC AACAATAACA TTGAATCTGA GAGGCCAGGC CCTTCCCACC 
     2401 AGCCCACCCC ACACAGCAGT GAGAACAACT CCAACCTGTC CCGTGGCCAC CTGAATCGCT 
     2461 GTCGTGCTTG CCACAATCTC CTGACCTTCA ACAACGATAC CCTGCGCTGG GAAAGAACCA 
     2521 CACCTAACTA CTCCTCTGGC GAGGCTAGTT CCTCTTGGCA GGTCCCCAGC TCCTTTGAGA 
     2581 GTGTGCCATC AAGTGGCAGC CAGTTGCCAC CTCTCGAGCG GACTGAGGGC CAAACGCCCA 
     2641 GCTCCAGCAG GCTGGAGTTG AGCAGCTCTG CTAGTCCGCA GGAGGAGAGG ACTGTGGGGG 
     2701 TGGCCTTTAA CCAGGAGACA GGCCACTGGG AAAGAATTTA CACCCAGTCC AGCAGATCTG 
     2761 GAACTGTGTC ACAGGAGGCC TTACATCAGG ATATGCCTGA GGAGAGCTCT GAGGAGGATT 
     2821 CACTCAGGAG GAGGCTGCTG GAATCTTCCC TCATTTCATT ATCCCGTTAT GATGGAGCAG 
     2881 GATCCAGAGA GCACCCAATT TACCCAGACC CAGCGAGATT ATCTCCTGCT GCATACTACG 
     2941 CCCAGAGGAT GATCCAGTAT CTCTCACGGA GAGACAGTAT TCGCCAGCGC TCCATGCGCT 
     3001 ACCAACAGAA CCGTCTCCGT TCTTCCACCT CCTCCTCTTC CTCAGACAAC CAGGGTCCAT 
     3061 CAGTAGAGGG AACCGACTTG GAATTTGAGG ACTTTGAGGA CAATGGTGAC AGATCCAGGC 
     3121 ACCGAGCTCC ACGCAATGCC CGGATGTCTG CACCTTCGCT TGGACGCTTT GTCCCAAGGC 
     3181 GTTTCTTGCT GCCTGAGTAC TTGCCTTATG CTGGGATTTT TCATGAACGT GGACAGCCTG 
     3241 GCTTGGCTAC TCACTCTTCT GTTAACAGGG TCCTGGCAGG GGCAGTGATC GGTGATGGAC 
     3301 AGTCTGCTGT GGCCAGTAAC ATTGCCAATA CTACCTACCG GCTCCAGTGG TGGGACTTCA 
     3361 CTAAGTTTGA CCTCCCTGAA ATCAGTAATG CTTCCGTGAA TGTGCTGGTG CAGAACTGCA 
     3421 AGATCTACAA TGATGCCAGC TGTGACATTT CTGCAGATGG CCAGCTCCTG GCAGCTTTCA 
     3481 TCCCCAGCAG CCAGAGGGGC TTTCCTGATG AAGGCATCCT GGCAGTGTAC TCCCTGGCCC 
     3541 CCCATAACCT GGGCGAAATG CTCTACACCA AGCGATTTGG TCCCAATGCC ATTTCGGTGA 
     3601 GCCTGTCCCC AATGGGCAGA TATGTAATGG TGGGCTTGGC CTCACGAAGG ATCCTGCTGC 
     3661 ACCCCTCCAC AGAGCACATG GTGGCCCAGG TCTTCAGGCT GCAACAGGCC CATGGTGGAG 
     3721 AGACCTCCAT GAGGAGAGTT TTCAACGTCC TTTATCCCAT GCCTGCCGAC CAGCGGAGAC 
     3781 ATGTCAGTAT CAACTCTGCC CGTTGGCTGC CTGAGCCAGG GCTTGGCTTG GCCTATGGTA 
     3841 CTAACAAAGG AGACCTGGTG ATCTGCCGAC CAGAGGCCTT AAACTCTGGT GTTGAGTACT 
     3901 ACTGGGACCA GCTGAACGAG ACGGTCTTCA CTGTCCATTC CAACAGCAGG AGCAGCGAGC 
     3961 GGCCTGGAAC CAGCAGAGCC ACATGGAGGA CAGACAGAGA CATGGGGCTG ATGAATGCCA 
     4021 TTGGGCTTCA GCCCCGGAAC CCTGCCACCT CAGTGACATC TCAGGGCACC GAGACTCTGG 
     4081 CCCTTCAGCT GCAGAATGCC GAAACACAGA CTGAGAGGGA GGTGCCGGAG CCAGGGACAG 
     4141 CCGCCTCAGG TCCTGGTGAA GGTGAGGGTT CAGAGTATGG TGCCAGTGGA GAAGATGCGC 
     4201 TCAGCAGGAT CCAGAGGCTG ATGGCGGAGG GCGGCATGAC AGCCGTGGTG CAGCGGGAGC 
     4261 AGAGCACCAC CATGGCCTCC ATGGGCGGCT TCGGCAACAA CATCATCGTC AGCCACCGCA 
     4321 TTCACCGCAG CTCTCAGACG GGCACTGAAC CTGGTGCCGC CCACACCTCC TCACCCCAGC 
     4381 CCTCCACCTC TCGGGGACTG CTCCCAGAGG CCGGGCAACT GGCAGAGCGA GGCCTAAGCC 
     4441 CCCGGACAGC TTCCTGGGAC CAGCCTGGTA CCCCTGGGCG GGAGCCAACC CAGCCAACCC 
     4501 TGCCCTCTTC CTCCCCTGTC CCCATTCCTG TTTCCCTTCC CAGCGCTGAG GGACCAACCG 
     4561 TCCACTGCGA GTTGACCAAT AACAACCACC TTCTGGATGG TGGCAGCAGC AGGGGGGACG 
     4621 CTGCAGGCCC TAGGGGAGAA CCACGGAACA GGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4681 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4741 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4801 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4861 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4921 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4981 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5041 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5101 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5161 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5221 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5281 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5341 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5401 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5461 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5521 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5581 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5641 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5701 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5761 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5821 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5881 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5941 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6001 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6061 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6121 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6181 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6241 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6301 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6361 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6421 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6481 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6541 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6601 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6661 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6721 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6781 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6841 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6901 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6961 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7021 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7081 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7141 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7201 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7261 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7321 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7381 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7441 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7501 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7561 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7621 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7681 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7741 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7861 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7921 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7981 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8041 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8101 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8161 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8221 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8281 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8341 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8401 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8461 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//