LOCUS       RC206047                7836 bp    DNA     circular  SYN 
DEFINITION  CBLB (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence b (CBLB)
ACCESSION   NM_170662
VERSION     NM_170662.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3991..4082 
                     /label="Myc-DDK"  
     misc_feature    4112..4697 
                     /label="PolyA signal"  
     misc_feature    4169..4188 
                     /label="Restriction Enzyme"  
     misc_feature    4973..5645 
                     /label="ColE1"  
     misc_feature    6075..6869 
                     /label="Kan/Neo"  
     misc_feature    6904..7206 
                     /label="SV40 ori"  
     misc_feature    7292..7769 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3966..3973 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3966 
                     /label="CBLB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAACTCA ATGAATGGCA GAAACCCTGG TGGTCGAGGA GGAAATCCCC 
     1081 GAAAAGGTCG AATTTTGGGT ATTATTGATG CTATTCAGGA TGCAGTTGGA CCCCCTAAGC 
     1141 AAGCTGCCGC AGATCGCAGG ACCGTGGAGA AGACTTGGAA GCTCATGGAC AAAGTGGTAA 
     1201 GACTGTGCCA AAATCCCAAA CTTCAGTTGA AAAATAGCCC ACCATATATA CTTGATATTT 
     1261 TGCCTGATAC ATATCAGCAT TTACGACTTA TATTGAGTAA ATATGATGAC AACCAGAAAC 
     1321 TTGCCCAACT CAGTGAGAAT GAGTACTTTA AAATCTACAT TGATAGCCTT ATGAAAAAGT 
     1381 CAAAACGGGC AATAAGACTC TTTAAAGAAG GCAAGGAGAG AATGTATGAA GAACAGTCAC 
     1441 AGGACAGACG AAATCTCACA AAACTGTCCC TTATCTTCAG TCACATGCTG GCAGAAATCA 
     1501 AAGCAATCTT TCCCAATGGT CAATTCCAGG GAGATAACTT TCGTATCACA AAAGCAGATG 
     1561 CTGCTGAATT CTGGAGAAAG TTTTTTGGAG ACAAAACTAT CGTACCATGG AAAGTATTCA 
     1621 GACAGTGCCT TCATGAGGTC CACCAGATTA GCTCTGGCCT GGAAGCAATG GCTCTAAAAT 
     1681 CAACAATTGA TTTAACTTGC AATGATTACA TTTCAGTTTT TGAATTTGAT ATTTTTACCA 
     1741 GGCTGTTTCA GCCTTGGGGC TCTATTTTGC GGAATTGGAA TTTCTTAGCT GTGACACATC 
     1801 CAGGTTACAT GGCATTTCTC ACATATGATG AAGTTAAAGC ACGACTACAG AAATATAGCA 
     1861 CCAAACCCGG AAGCTATATT TTCCGGTTAA GTTGCACTCG ATTGGGACAG TGGGCCATTG 
     1921 GCTATGTGAC TGGGGATGGG AATATCTTAC AGACCATACC TCATAACAAG CCCTTATTTC 
     1981 AAGCCCTGAT TGATGGCAGC AGGGAAGGAT TTTATCTTTA TCCTGATGGG AGGAGTTATA 
     2041 ATCCTGATTT AACTGGATTA TGTGAACCTA CACCTCATGA CCATATAAAA GTTACACAGG 
     2101 AACAATATGA ATTATATTGT GAAATGGGCT CCACTTTTCA GCTCTGTAAG ATTTGTGCAG 
     2161 AGAATGACAA AGATGTCAAG ATTGAGCCTT GTGGGCATTT GATGTGCACC TCTTGCCTTA 
     2221 CGGCATGGCA GGAGTCGGAT GGTCAGGGCT GCCCTTTCTG TCGTTGTGAA ATAAAAGGAA 
     2281 CTGAGCCCAT AATCGTGGAT CCCTTTGATC CAAGAGATGA AGGCTCCAGG TGTTGCAGCA 
     2341 TCATTGACCC CTTTGGCATG CCGATGCTCG ACTTGGACGA CGATGATGAT CGTGAGGAGT 
     2401 CCTTGATGAT GAATCGGTTG GCAAACGTCC GAAAGTGCAC TGACAGGCAG AACTCACCAG 
     2461 TCACATCACC AGGATCCTCT CCCCTTGCCC AGAGAAGAAA GCCACAGCCT GACCCACTCC 
     2521 AGATCCCACA TCTAAGCCTG CCACCCGTGC CTCCTCGCCT GGATCTAATT CAGAAAGGCA 
     2581 TAGTTAGATC TCCCTGTGGC AGCCCAACGG GTTCACCAAA GTCTTCTCCT TGCATGGTGA 
     2641 GAAAACAAGA TAAACCACTC CCAGCACCAC CTCCTCCCTT AAGAGATCCT CCTCCACCGC 
     2701 CACCTGAAAG ACCTCCACCA ATCCCACCAG ACAATAGACT GAGTAGACAC ATCCATCATG 
     2761 TGGAAAGCGT GCCTTCCAAA GACCCGCCAA TGCCTCTTGA AGCATGGTGC CCTCGGGATG 
     2821 TGTTTGGGAC TAATCAGCTT GTGGGATGTC GACTCCTAGG GGAGGGCTCT CCAAAACCTG 
     2881 GAATCACAGC GAGTTCAAAT GTCAATGGAA GGCACAGTAG AGTGGGCTCT GACCCAGTGC 
     2941 TTATGCGGAA ACACAGACGC CATGATTTGC CTTTAGAAGG AGCTAAGGTC TTTTCCAATG 
     3001 GTCACCTTGG AAGTGAAGAA TATGATGTTC CTCCCCGGCT TTCTCCTCCT CCTCCAGTTA 
     3061 CCACCCTCCT CCCTAGCATA AAGTGTACTG GTCCGTTAGC AAATTCTCTT TCAGAGAAAA 
     3121 CAAGAGACCC AGTAGAGGAA GATGATGATG AATACAAGAT TCCTTCATCC CACCCTGTTT 
     3181 CCCTGAATTC ACAACCATCT CATTGTCATA ATGTAAAACC TCCTGTTCGG TCTTGTGATA 
     3241 ATGGTCACTG TATGCTGAAT GGAACACATG GTCCATCTTC AGAGAAGAAA TCAAACATCC 
     3301 CTGACTTAAG CATATATTTA AAGGGAGATG TTTTTGATTC AGCCTCTGAT CCCGTGCCAT 
     3361 TACCACCTGC CAGGCCTCCA ACTCGGGACA ATCCAAAGCA TGGTTCTTCA CTCAACAGGA 
     3421 CGCCCTCTGA TTATGATCTT CTCATCCCTC CATTAGGTGA AGATGCTTTT GATGCCCTCC 
     3481 CTCCATCTCT CCCACCTCCC CCACCTCCTG CAAGGCATAG TCTCATTGAA CATTCAAAAC 
     3541 CTCCTGGCTC CAGTAGCCGG CCATCCTCAG GACAGGATCT TTTTCTTCTT CCTTCAGATC 
     3601 CCTTTGTTGA TCTAGCAAGT GGCCAAGTTC CTTTGCCTCC CGCTAGAAGG TTACCAGGTG 
     3661 AAAATGTCAA AACTAACAGA ACATCACAGG ACTATGATCA GCTTCCTTCA TGTTCAGATG 
     3721 GTTCACAGGC ACCAGCCAGA CCCCCTAAAC CACGACCGCG CAGGACTGCA CCAGAAATTC 
     3781 ACCACAGAAA ACCCCATGGG CCTGAGGCGG CATTGGAAAA TGTCGATGCA AAAATTGCAA 
     3841 AACTCATGGG AGAGGGTTAT GCCTTTGAAG AGGTGAAGAG AGCCTTAGAG ATAGCCCAGA 
     3901 ATAATGTCGA AGTTGCCCGG AGCATCCTCC GAGAATTTGC CTTCCCTCCT CCAGTATCCC 
     3961 CACGTCTAAA TCTAAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4021 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4081 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4141 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4201 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4261 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4321 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4381 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4441 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4501 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4561 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4621 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4681 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4741 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4801 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4861 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4921 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4981 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5041 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5101 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5161 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5221 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5281 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5341 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5401 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5461 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5521 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5581 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5641 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5701 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5761 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5821 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5881 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5941 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6001 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6061 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6121 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6181 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6241 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6301 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6361 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6421 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6481 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6541 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6601 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6661 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6721 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6781 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6841 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6901 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6961 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7021 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7081 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7261 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7321 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7381 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7441 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7501 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7561 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7621 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7681 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7741 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7801 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//