LOCUS       RC206026                9393 bp    DNA     circular  SYN 
DEFINITION  NISCH (Myc-DDK-tagged)-Human nischarin (NISCH)
ACCESSION   NM_007184
VERSION     NM_007184.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5550..5641 
                     /label="Myc-DDK"  
     misc_feature    5671..6256 
                     /label="PolyA signal"  
     misc_feature    5728..5747 
                     /label="Restriction Enzyme"  
     misc_feature    6532..7204 
                     /label="ColE1"  
     misc_feature    7634..8428 
                     /label="Kan/Neo"  
     misc_feature    8463..8765 
                     /label="SV40 ori"  
     misc_feature    8851..9328 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5532..5538 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5532 
                     /label="NISCH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACCGCG CGCACCTTCG GGCCCGAGCG GGAAGCCGAG CCGGCCAAGG 
     1081 AAGCGCGCGT CGTGGGCTCG GAGCTTGTGG ACACTTATAC GGTTTACATC ATCCAGGTCA 
     1141 CTGATGGCAG CCATGAGTGG ACAGTAAAGC ACCGCTACAG CGACTTCCAT GACCTGCATG 
     1201 AAAAGCTCGT TGCAGAGAGA AAGATTGATA AAAATCTGCT TCCGCCCAAA AAGATAATTG 
     1261 GGAAAAACTC AAGAAGCTTG GTGGAGAAGA GGGAGAAGGA TCTGGAGGTC TACCTCCAGA 
     1321 AGCTCCTGGC TGCCTTCCCT GGCGTGACCC CCAGAGTACT GGCCCACTTC TTGCATTTTC 
     1381 ACTTCTATGA GATAAATGGC ATCACCGCGG CACTGGCTGA AGAGCTCTTT GAGAAAGGAG 
     1441 AACAGCTCCT GGGGGCCGGC GAGGTCTTTG CCATTGGACC CCTGCAGCTG TATGCCGTCA 
     1501 CGGAGCAGCT GCAGCAGGGA AAGCCCACGT GCGCCAGTGG GGATGCCAAG ACCGACCTCG 
     1561 GGCACATCCT GGACTTCACC TGTCGCCTTA AGTACCTTAA GGTTTCTGGC ACAGAAGGAC 
     1621 CTTTTGGGAC CAGCAACATT CAGGAGCAGC TCCTGCCGTT CGACCTATCA ATATTCAAGT 
     1681 CCCTGCATCA GGTGGAGATA AGTCACTGTG ATGCTAAGCA CATCAGAGGG CTGGTCGCAT 
     1741 CGAAGCCCAC CTTAGCCACG CTGAGTGTCC GCTTCTCAGC AACCTCGATG AAGGAAGTCC 
     1801 TTGTTCCTGA AGCCTCAGAA TTTGATGAGT GGGAGCCTGA AGGCACAACC CTAGAAGGCC 
     1861 CTGTGACTGC CGTCATCCCC ACTTGGCAGG CATTGACCAC GCTTGACCTG AGCCACAACA 
     1921 GCATCTCCGA GATCGACGAG TCTGTGAAAC TGATCCCAAA GATTGAGTTC CTGGACCTGA 
     1981 GTCACAATGG ATTGCTGGTT GTGGACAATC TGCAGCACCT GTATAACCTT GTGCATCTGG 
     2041 ACCTGTCCTA CAACAAGCTC TCCTCCTTGG AAGGGCTTCA CACCAAGCTG GGGAACATCA 
     2101 AGACCTTAAA CCTGGCAGGC AACCTCCTAG AGAGTCTGAG TGGCCTGCAC AAGCTCTACT 
     2161 CACTGGTCAA CCTGGATCTC CGGGACAACA GGATCGAACA GATGGAGGAG GTCCGGAGCA 
     2221 TAGGCAGCCT CCCGTGTCTG GAGCACGTGT CTCTGCTGAA CAACCCTCTG AGCATCATCC 
     2281 CCGACTACCG GACCAAGGTG CTGGCTCAGT TCGGAGAGAG GGCCTCAGAG GTCTGTCTGG 
     2341 ATGACACAGT GACCACAGAG AAGGAGCTGG ACACTGTGGA AGTGCTGAAA GCAATTCAGA 
     2401 AAGCCAAGGA GGTCAAGTCC AAACTGAGCA ACCCAGAGAA GAAGGGTGGT GAAGACTCCC 
     2461 GGCTCTCAGC TGCCCCCTGC ATCAGACCCA GCAGCTCCCC TCCCACTGTG GCTCCCGCAT 
     2521 CTGCCTCCCT GCCCCAGCCC ATCCTCTCTA ACCAAGGAAT CATGTTCGTT CAGGAGGAGG 
     2581 CCCTGGCCAG CAGCCTCTCG TCCACTGACA GTCTGACTCC CGAGCACCAG CCCATTGCCC 
     2641 AGGGATGTTC TGATTCCTTG GAGTCCATCC CTGCGGGACA GGCAGCTTCC GATGATTTAA 
     2701 GGGACGTGCC AGGAGCTGTT GGTGGTGCAA GCCCAGAACA TGCCGAGCCG GAGGTCCAGG 
     2761 TGGTGCCGGG GTCTGGCCAG ATCATCTTCC TGCCCTTCAC CTGCATTGGC TACACGGCCA 
     2821 CCAATCAGGA CTTCATCCAG CGCCTGAGCA CACTGATCCG GCAGGCCATC GAGCGGCAGC 
     2881 TGCCTGCCTG GATCGAGGCT GCCAACCAGC GGGAGGAGGG CCAGGGTGAA CAGGGCGAGG 
     2941 AGGAGGATGA GGAGGAGGAA GAAGAGGAGG ACGTGGCTGA GAACCGCTAC TTTGAAATGG 
     3001 GGCCCCCAGA CGTGGAGGAG GAGGAGGGAG GAGGCCAGGG GGAGGAAGAG GAGGAGGAAG 
     3061 AGGAGGATGA AGAGGCCGAG GAGGAGCGCC TGGCTCTGGA ATGGGCCCTG GGCGCGGACG 
     3121 AGGACTTCCT GCTGGAGCAC ATCCGCATCC TCAAGGTGCT GTGGTGCTTC CTGATCCATG 
     3181 TGCAGGGCAG TATCCGCCAG TTCGCCGCCT GCCTTGTGCT CACCGACTTC GGCATCGCAG 
     3241 TCTTCGAGAT CCCGCACCAG GAGTCTCGGG GCAGCAGCCA GCACATCCTC TCCTCCCTGC 
     3301 GCTTTGTCTT TTGCTTCCCG CATGGCGACC TCACCGAGTT TGGCTTCCTC ATGCCGGAGC 
     3361 TGTGTCTGGT GCTCAAGGTA CGGCACAGTG AGAACACGCT CTTCATTATC TCGGACGCCG 
     3421 CCAACCTGCA CGAGTTCCAC GCGGACCTGC GCTCATGCTT TGCACCCCAG CACATGGCCA 
     3481 TGCTGTGTAG CCCCATCCTC TACGGCAGCC ACACCAGCCT GCAGGAGTTC CTGCGCCAGC 
     3541 TGCTCACCTT CTACAAGGTG GCTGGCGGCT GCCAGGAGCG CAGCCAGGGC TGCTTCCCCG 
     3601 TCTACCTGGT CTACAGTGAC AAGCGCATGG TGCAGACGGC CGCCGGGGAC TACTCAGGCA 
     3661 ACATCGAGTG GGCCAGCTGC ACACTCTGTT CAGCCGTGCG GCGCTCCTGC TGCGCGCCCT 
     3721 CTGAGGCCGT CAAGTCCGCC GCCATCCCCT ACTGGCTGTT GCTCACGCCC CAGCACCTCA 
     3781 ACGTCATCAA GGCCGACTTC AACCCCATGC CCAACCGTGG CACCCACAAC TGTCGCAACC 
     3841 GCAACAGCTT CAAGCTCAGC CGTGTGCCGC TCTCCACCGT GCTGCTGGAC CCCACACGCA 
     3901 GCTGTACCCA GCCTCGGGGC GCCTTTGCTG ATGGCCACGT GCTAGAGCTG CTCGTGGGGT 
     3961 ACCGCTTTGT CACTGCCATC TTCGTGCTGC CCCACGAGAA GTTCCACTTC CTGCGCGTCT 
     4021 ACAACCAGCT GCGGGCCTCG CTGCAGGACC TGAAGACTGT GGTCATCGCC AAGACCCCCG 
     4081 GGACGGGAGG CAGCCCCCAG GGCTCCTTTG CGGATGGCCA GCCTGCCGAG CGCAGGGCCA 
     4141 GCAATGACCA GCGTCCCCAG GAGGTCCCAG CAGAGGCTCT GGCCCCGGCC CCAGCGGAAG 
     4201 TCCCAGCTCC AGCCCCTGCA GCAGCCTCAG CCTCAGGCCC AGCGAAGACT CCGGCCCCAG 
     4261 CAGAGGCCTC AACTTCAGCT TTGGTCCCAG AGGAGACGCC AGTGGAAGCT CCAGCCCCAC 
     4321 CCCCAGCCGA GGCCCCTGCC CAGTACCCGA GTGAGCACCT CATCCAGGCC ACCTCGGAGG 
     4381 AGAATCAGAT CCCCTCGCAC TTGCCTGCCT GCCCGTCGCT CCGGCACGTC GCCAGCCTGC 
     4441 GGGGCAGCGC CATCATCGAG CTCTTCCACA GCAGCATTGC TGAGGTTGAA AACGAGGAGC 
     4501 TGAGGCACCT CATGTGGTCC TCGGTGGTGT TCTACCAGAC CCCAGGGCTG GAGGTGACTG 
     4561 CCTGCGTGCT GCTCTCCACC AAGGCTGTGT ACTTTGTGCT CCACGACGGC CTCCGCCGCT 
     4621 ACTTCTCAGA GCCACTGCAG GATTTCTGGC ATCAGAAAAA CACCGACTAC AACAACAGCC 
     4681 CTTTCCACAT CTCCCAGTGC TTCGTGCTAA AGCTTAGTGA CCTGCAGTCA GTCAATGTGG 
     4741 GGCTTTTCGA CCAGCATTTC CGGCTGACGG GTTCCACCCC GATGCAGGTG GTCACGTGCT 
     4801 TGACGCGGGA CAGCTACCTG ACGCACTGCT TCCTCCAGCA CCTCATGGTC GTGCTGTCCT 
     4861 CTCTGGAACG CACGCCCTCG CCGGAGCCTG TTGACAAGGA CTTCTACTCC GAGTTTGGGA 
     4921 ACAAGACCAC AGGGAAGATG GAGAACTACG AGCTGATCCA CTCTAGTCGC GTCAAGTTTA 
     4981 CCTACCCCAG TGAGGAGGAG ATTGGGGACC TGACGTTCAC TGTGGCCCAA AAGATGGCTG 
     5041 AGCCAGAGAA GGCCCCAGCC CTCAGCATCC TGCTGTACGT GCAGGCCTTC CAGGTGGGCA 
     5101 TGCCACCCCC TGGGTGCTGC AGGGGCCCCC TGCGCCCCAA GACACTCCTG CTCACCAGCT 
     5161 CCGAGATCTT CCTCCTGGAT GAGGACTGTG TCCACTACCC ACTGCCCGAG TTTGCCAAAG 
     5221 AGCCGCCGCA GAGAGACAGG TACCGGCTGG ACGATGGCCG CCGCGTCCGG GACCTGGACC 
     5281 GAGTGCTCAT GGGCTACCAG ACCTACCCGC AGGCCCTCAC CCTCGTCTTC GATGACGTGC 
     5341 AAGGTCATGA CCTCATGGGC AGTGTCACCC TGGACCACTT TGGGGAGGTG CCAGGTGGCC 
     5401 CGGCTAGAGC CAGCCAGGGC CGTGAAGTCC AGTGGCAGGT GTTTGTCCCC AGTGCTGAGA 
     5461 GCAGAGAGAA GCTCATCTCG CTGTTGGCTC GCCAGTGGGA GGCCCTGTGT GGCCGTGAGC 
     5521 TGCCTGTCGA GCTCACCGGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5581 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5641 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5701 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5761 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5821 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5881 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5941 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     6001 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6061 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6121 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6181 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6241 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6301 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6361 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6421 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6481 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6541 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6601 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6661 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6721 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6781 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6841 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6901 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6961 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     7021 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7081 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7141 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7201 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7261 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7321 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7381 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7441 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7501 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7561 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7621 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7681 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7741 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7801 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7861 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7921 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7981 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     8041 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8101 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8161 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8221 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8281 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8341 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8401 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8461 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8521 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8581 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8641 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8701 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8761 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8821 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8881 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8941 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     9001 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9061 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9121 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9181 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9241 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9301 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9361 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//