LOCUS       RC205984                7782 bp    DNA     circular  SYN 
DEFINITION  ADAMTS1 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 1 (ADAMTS1)
ACCESSION   NM_006988
VERSION     NM_006988.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3939..4030 
                     /label="Myc-DDK"  
     misc_feature    4060..4645 
                     /label="PolyA signal"  
     misc_feature    4117..4136 
                     /label="Restriction Enzyme"  
     misc_feature    4921..5593 
                     /label="ColE1"  
     misc_feature    6023..6817 
                     /label="Kan/Neo"  
     misc_feature    6852..7154 
                     /label="SV40 ori"  
     misc_feature    7240..7717 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3921..3927 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3921 
                     /label="ADAMTS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCGAGCT GTGCCCGAGG GGTTCGGAAG GCGCAAGCTG GGCAGCGACA 
     1081 TGGGGAACGC GGAGCGGGCT CCGGGGTCTC GGAGCTTTGG GCCCGTACCC ACGCTGCTGC 
     1141 TGCTCGCCGC GGCGCTACTG GCCGTGTCGG ACGCACTCGG GCGCCCCTCC GAGGAGGACG 
     1201 AGGAGCTAGT GGTGCCGGAG CTGGAGCGCG CCCCGGGACA CGGGACCACG CGCCTCCGCC 
     1261 TGCACGCCTT TGACCAGCAG CTGGATCTGG AGCTGCGGCC CGACAGCAGC TTTTTGGCGC 
     1321 CCGGCTTCAC GCTCCAGAAC GTGGGGCGCA AATCCGGGTC CGAGACGCCG CTTCCGGAAA 
     1381 CCGACCTGGC GCACTGCTTC TACTCCGGCA CCGTGAATGG CGATCCCAGC TCGGCTGCCG 
     1441 CCCTCAGCCT CTGCGAGGGC GTGCGCGGCG CCTTCTACCT GCTGGGGGAG GCGTATTTCA 
     1501 TCCAGCCGCT GCCCGCCGCC AGCGAGCGCC TCGCCACCGC CGCCCCAGGG GAGAAGCCGC 
     1561 CGGCACCACT ACAGTTCCAC CTCCTGCGGC GGAATCGGCA GGGCGACGTA GGCGGCACGT 
     1621 GCGGGGTCGT GGACGACGAG CCCCGGCCGA CTGGGAAAGC GGAGACCGAA GACGAGGACG 
     1681 AAGGGACTGA GGGCGAGGAC GAAGGGCCTC AGTGGTCGCC GCAGGACCCG GCACTGCAAG 
     1741 GCGTAGGACA GCCCACAGGA ACTGGAAGCA TAAGAAAGAA GCGATTTGTG TCCAGTCACC 
     1801 GCTATGTGGA AACCATGCTT GTGGCAGACC AGTCGATGGC AGAATTCCAC GGCAGTGGTC 
     1861 TAAAGCATTA CCTTCTCACG TTGTTTTCGG TGGCAGCCAG ATTGTACAAA CACCCCAGCA 
     1921 TTCGTAATTC AGTTAGCCTG GTGGTGGTGA AGATCTTGGT CATCCACGAT GAACAGAAGG 
     1981 GGCCGGAAGT GACCTCCAAT GCTGCCCTCA CTCTGCGGAA CTTTTGCAAC TGGCAGAAGC 
     2041 AGCACAACCC ACCCAGTGAC CGGGATGCAG AGCACTATGA CACAGCAATT CTTTTCACCA 
     2101 GACAGGACTT GTGTGGGTCC CAGACATGTG ATACTCTTGG GATGGCTGAT GTTGGAACTG 
     2161 TGTGTGATCC GAGCAGAAGC TGCTCCGTCA TAGAAGATGA TGGTTTACAA GCTGCCTTCA 
     2221 CCACAGCCCA TGAATTAGGC CACGTGTTTA ACATGCCACA TGATGATGCA AAGCAGTGTG 
     2281 CCAGCCTTAA TGGTGTGAAC CAGGATTCCC ACATGATGGC GTCAATGCTT TCCAACCTGG 
     2341 ACCACAGCCA GCCTTGGTCT CCTTGCAGTG CCTACATGAT TACATCATTT CTGGATAATG 
     2401 GTCATGGGGA ATGTTTGATG GACAAGCCTC AGAATCCCAT ACAGCTCCCA GGCGATCTCC 
     2461 CTGGCACCTC GTACGATGCC AACCGGCAGT GCCAGTTTAC ATTTGGGGAG GACTCCAAAC 
     2521 ACTGCCCTGA TGCAGCCAGC ACATGTAGCA CCTTGTGGTG TACCGGCACC TCTGGTGGGG 
     2581 TGCTGGTGTG TCAAACCAAA CACTTCCCGT GGGCGGATGG CACCAGCTGT GGAGAAGGGA 
     2641 AATGGTGTAT CAACGGCAAG TGTGTGAACA AAACCGACAG AAAGCATTTT GATACGCCTT 
     2701 TTCATGGAAG CTGGGGAATG TGGGGGCCTT GGGGAGACTG TTCGAGAACG TGCGGTGGAG 
     2761 GAGTCCAGTA CACGATGAGG GAATGTGACA ACCCAGTCCC AAAGAATGGA GGGAAGTACT 
     2821 GTGAAGGCAA ACGAGTGCGC TACAGATCCT GTAACCTTGA GGACTGTCCA GACAATAATG 
     2881 GAAAAACCTT TAGAGAGGAA CAATGTGAAG CACACAACGA GTTTTCAAAA GCTTCCTTTG 
     2941 GGAGTGGGCC TGCGGTGGAA TGGATTCCCA AGTACGCTGG CGTCTCACCA AAGGACAGGT 
     3001 GCAAGCTCAT CTGCCAAGCC AAAGGCATTG GCTACTTCTT CGTTTTGCAG CCCAAGGTTG 
     3061 TAGATGGTAC TCCATGTAGC ACAGATTCCA CCTCTGTCTG TGTGCAAGGA CAGTGTGTAA 
     3121 AAGCTGGTTG TGATCGCATC ATAGACTCCA AAAAGAAGTT TGATAAATGT GGTGTTTGCG 
     3181 GGGGAAATGG ATCTACTTGT AAAAAAATAT CAGGATCAGT TACTAGTGCA AAACCTGGAT 
     3241 ATCATGATAT CATCACAATT CCAACTGGAG CCACCAACAT CGAAGTGAAA CAGCGGAACC 
     3301 AGAGGGGATC CAGGAACAAT GGCAGCTTTC TTGCCATCAA AGCTGCTGAT GGCACATATA 
     3361 TTCTTAATGG TGACTACACT TTGTCCACCT TAGAGCAAGA CATTATGTAC AAAGGTGTTG 
     3421 TCTTGAGGTA CAGCGGCTCC TCTGCGGCAT TGGAAAGAAT TCGCAGCTTT AGCCCTCTCA 
     3481 AAGAGCCCTT GACCATCCAG GTTCTTACTG TGGGCAATGC CCTTCGACCT AAAATTAAAT 
     3541 ACACCTACTT CGTAAAGAAG AAGAAGGAAT CTTTCAATGC TATCCCCACT TTTTCAGCAT 
     3601 GGGTCATTGA AGAGTGGGGC GAATGTTCTA AGTCATGTGA ATTGGGTTGG CAGAGAAGAC 
     3661 TGGTAGAATG CCGAGACATT AATGGACAGC CTGCTTCCGA GTGTGCAAAG GAAGTGAAGC 
     3721 CAGCCAGCAC CAGACCTTGT GCAGACCATC CCTGCCCCCA GTGGCAGCTG GGGGAGTGGT 
     3781 CATCATGTTC TAAGACCTGT GGGAAGGGTT ACAAAAAAAG AAGCTTGAAG TGTCTGTCCC 
     3841 ATGATGGAGG GGTGTTATCT CATGAGAGCT GTGATCCTTT AAAGAAACCT AAACATTTCA 
     3901 TAGACTTTTG CACAATGGCA GAATGCAGTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3961 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4021 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4081 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4141 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4201 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4261 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4321 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4381 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4441 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4501 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4561 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4621 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4681 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4741 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4801 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4861 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4921 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4981 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5041 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5101 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5161 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5221 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5281 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5341 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5401 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5461 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5521 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5581 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5641 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5701 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5761 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5821 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5881 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5941 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6001 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6061 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6121 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6181 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6241 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6301 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6361 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6421 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6481 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6541 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6601 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6661 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6721 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6781 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6841 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6901 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6961 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7021 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7081 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7141 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7201 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7261 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7321 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7381 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7441 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7501 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7561 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7621 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7681 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7741 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//