LOCUS       RC205768                6891 bp    DNA     circular  SYN 
DEFINITION  DDX18 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 (DDX18)
ACCESSION   NM_006773
VERSION     NM_006773.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3048..3139 
                     /label="Myc-DDK"  
     misc_feature    3169..3754 
                     /label="PolyA signal"  
     misc_feature    3226..3245 
                     /label="Restriction Enzyme"  
     misc_feature    4030..4702 
                     /label="ColE1"  
     misc_feature    5132..5926 
                     /label="Kan/Neo"  
     misc_feature    5961..6263 
                     /label="SV40 ori"  
     misc_feature    6349..6826 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3030..3036 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3030 
                     /label="DDX18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACACCTG CCGATGAAAC TCCTGCGTAA GAAGATCGAG AAGCGGAACC 
     1081 TCAAATTGCG GCAGCGGAAC CTAAAGTTTC AGGGGGCCTC AAATCTGACC CTATCGGAAA 
     1141 CTCAAAATGG AGATGTATCT GAAGAAACAA TGGGAAGTAG AAAGGTTAAA AAATCAAAAC 
     1201 AAAAGCCCAT GAATGTGGGC TTATCAGAAA CTCAAAATGG AGGCATGTCT CAAGAAGCAG 
     1261 TGGGAAATAT AAAAGTTACA AAGTCTCCCC AGAAATCCAC TGTATTAAGC AATGGAGAAG 
     1321 CAGCAATGCA GTCTTCCAAT TCAGAATCAA AAAAGAAAAA GAAGAAAAAG AGAAAAATGG 
     1381 TGAATGATGC TGAGCCTGAT ACGAAAAAAG CAAAAACTGA AAACAAAGGG AAATCTGAAG 
     1441 AAGAAAGTGC CGAGACTACT AAAGAAACAG AAAATAATGT GGAGAAGCCA GATAATGATG 
     1501 AAGATGAGAG TGAGGTGCCC AGTCTGCCCC TGGGACTGAC AGGAGCTTTT GAGGATACTT 
     1561 CGTTTGCTTC TCTATGTAAT CTTGTCAATG AAAACACTCT GAAGGCAATA AAAGAAATGG 
     1621 GTTTTACAAA CATGACTGAA ATTCAGCATA AAAGTATCAG ACCACTTCTG GAAGGCAGGG 
     1681 ATCTTCTAGC AGCTGCAAAA ACAGGCAGTG GTAAAACCCT GGCTTTTCTC ATCCCTGCAG 
     1741 TTGAACTCAT TGTTAAGTTA AGGTTCATGC CCAGGAATGG AACAGGAGTC CTTATTCTCT 
     1801 CACCTACTAG AGAACTAGCC ATGCAAACCT TTGGTGTTCT TAAGGAGCTG ATGACTCACC 
     1861 ACGTGCATAC CTATGGCTTG ATAATGGGTG GCAGTAACAG ATCTGCTGAA GCACAGAAAC 
     1921 TTGGTAATGG GATCAACATC ATTGTGGCCA CACCAGGCCG TCTGCTGGAC CATATGCAGA 
     1981 ATACCCCAGG ATTTATGTAT AAAAACCTGC AGTGTCTGGT TATTGATGAA GCTGATCGTA 
     2041 TCTTGGATGT GGGGTTTGAA GAGGAATTAA AGCAAATTAT TAAACTTTTG CCAACACGTA 
     2101 GACAGACTAT GCTCTTTTCT GCCACCCAAA CTCGAAAAGT TGAAGACCTG GCAAGGATTT 
     2161 CTCTGAAAAA GGAGCCATTG TATGTTGGCG TTGATGATGA TAAAGCGAAT GCAACAGTGG 
     2221 ATGGTCTTGA ACAGGGATAT GTTGTTTGTC CTTCTGAAAA GAGATTCCTT CTGCTCTTTA 
     2281 CATTCCTTAA GAAGAACCGA AAGAAGAAGC TTATGGTCTT CTTTTCATCT TGTATGTCTG 
     2341 TGAAATACCA CTATGAGTTG CTGAACTACA TTGATTTGCC CGTCTTGGCC ATTCATGGAA 
     2401 AGCAAAAGCA AAATAAGCGT ACAACCACAT TCTTCCAGTT CTGCAATGCA GATTCGGGAA 
     2461 CACTATTGTG TACGGATGTG GCAGCGAGAG GACTAGACAT TCCTGAAGTC GACTGGATTG 
     2521 TTCAGTATGA CCCTCCGGAT GACCCTAAGG AATATATTCA TCGTGTGGGT AGAACAGCCA 
     2581 GAGGCCTAAA TGGGAGAGGG CATGCCTTGC TCATTTTGCG CCCAGAAGAA TTGGGTTTTC 
     2641 TTCGCTACTT GAAACAATCC AAGGTTCCAT TAAGTGAATT TGACTTTTCC TGGTCTAAAA 
     2701 TTTCTGACAT TCAGTCTCAG CTTGAGAAAT TGATTGAAAA GAATTACTTT CTTCATAAGT 
     2761 CAGCCCAGGA AGCATATAAG TCATACATAC GAGCCTATGA TTCCCATTCT CTGAAACAGA 
     2821 TCTTTAATGT TAATAACCTA AATTTGCCTC AGGTTGCTCT GTCATTTGGT TTCAAGGTGC 
     2881 CTCCCTTCGT TGATCTGAAC GTCAACAGTA ATGAAGGCAA GCAGAAAAAG CGAGGAGGTG 
     2941 GTGGTGGATT TGGCTACCAG AAAACCAAGA AAGTTGAGAA ATCCAAAATC TTTAAACACA 
     3001 TTAGCAAGAA ATCATCTGAC AGCAGGCAGT TCTCTCACAC GCGTACGCGG CCGCTCGAGC 
     3061 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3121 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3181 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3241 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3301 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3361 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3421 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3481 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3541 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3601 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3661 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3721 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3781 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3841 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3901 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     3961 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4021 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4081 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4141 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4201 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4261 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4321 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4381 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4441 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4501 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4561 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4621 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4681 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4741 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4801 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4861 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4921 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     4981 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5041 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5101 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5161 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5221 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5281 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5341 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5401 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5461 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5521 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5581 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5641 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5701 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5761 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5821 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5881 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     5941 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6001 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6061 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6121 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6181 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6241 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6301 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6361 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6421 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6481 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6541 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6601 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6661 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6721 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6781 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6841 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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