LOCUS       RC205725L1                9303 bp    DNA     circular  SYN 
DEFINITION  EPHA2 (Myc-DDK-tagged)-Human EPH receptor A2 (EPHA2)
ACCESSION   NM_004431
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7594..7774 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8502..9161 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7933..8501 
                     /label="ColE1"  
     misc_feature    6469..6489 
                     /label="LR50 primer"  
     misc_feature    6266..6337 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6478..7069 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6248..6254 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6248 
                     /label="EPHA2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGCTCCAG GCAGCCCGCG CCTGCTTCGC 
     3361 CCTGCTGTGG GGCTGTGCGC TGGCCGCGGC CGCGGCGGCG CAGGGCAAGG AAGTGGTACT 
     3421 GCTGGACTTT GCTGCAGCTG GAGGGGAGCT CGGCTGGCTC ACACACCCGT ATGGCAAAGG 
     3481 GTGGGACCTG ATGCAGAACA TCATGAATGA CATGCCGATC TACATGTACT CCGTGTGCAA 
     3541 CGTGATGTCT GGCGACCAGG ACAACTGGCT CCGCACCAAC TGGGTGTACC GAGGAGAGGC 
     3601 TGAGCGTATC TTCATTGAGC TCAAGTTTAC TGTACGTGAC TGCAACAGCT TCCCTGGTGG 
     3661 CGCCAGCTCC TGCAAGGAGA CTTTCAACCT CTACTATGCC GAGTCGGACC TGGACTACGG 
     3721 CACCAACTTC CAGAAGCGCC TGTTCACCAA GATTGACACC ATTGCGCCCG ATGAGATCAC 
     3781 CGTCAGCAGC GACTTCGAGG CACGCCACGT GAAGCTGAAC GTGGAGGAGC GCTCCGTGGG 
     3841 GCCGCTCACC CGCAAAGGCT TCTACCTGGC CTTCCAGGAT ATCGGTGCCT GTGTGGCGCT 
     3901 GCTCTCCGTC CGTGTCTACT ACAAGAAGTG CCCCGAGCTG CTGCAGGGCC TGGCCCACTT 
     3961 CCCTGAGACC ATCGCCGGCT CTGATGCACC TTCCCTGGCC ACTGTGGCCG GCACCTGTGT 
     4021 GGACCATGCC GTGGTGCCAC CGGGGGGTGA AGAGCCCCGT ATGCACTGTG CAGTGGATGG 
     4081 CGAGTGGCTG GTGCCCATTG GGCAGTGCCT GTGCCAGGCA GGCTACGAGA AGGTGGAGGA 
     4141 TGCCTGCCAG GCCTGCTCGC CTGGATTTTT TAAGTTTGAG GCATCTGAGA GCCCCTGCTT 
     4201 GGAGTGCCCT GAGCACACGC TGCCATCCCC TGAGGGTGCC ACCTCCTGCG AGTGTGAGGA 
     4261 AGGCTTCTTC CGGGCACCTC AGGACCCAGC GTCGATGCCT TGCACACGAC CCCCCTCCGC 
     4321 CCCACACTAC CTCACAGCCG TGGGCATGGG TGCCAAGGTG GAGCTGCGCT GGACGCCCCC 
     4381 TCAGGACAGC GGGGGCCGCG AGGACATTGT CTACAGCGTC ACCTGCGAAC AGTGCTGGCC 
     4441 CGAGTCTGGG GAATGCGGGC CGTGTGAGGC CAGTGTGCGC TACTCGGAGC CTCCTCACGG 
     4501 ACTGACCCGC ACCAGTGTGA CAGTGAGCGA CCTGGAGCCC CACATGAACT ACACCTTCAC 
     4561 CGTGGAGGCC CGCAATGGCG TCTCAGGCCT GGTAACCAGC CGCAGCTTCC GTACTGCCAG 
     4621 TGTCAGCATC AACCAGACAG AGCCCCCCAA GGTGAGGCTG GAGGGCCGCA GCACCACCTC 
     4681 GCTTAGCGTC TCCTGGAGCA TCCCCCCGCC GCAGCAGAGC CGAGTGTGGA AGTACGAGGT 
     4741 CACTTACCGC AAGAAGGGAG ACTCCAACAG CTACAATGTG CGCCGCACCG AGGGTTTCTC 
     4801 CGTGACCCTG GACGACCTGG CCCCAGACAC CACCTACCTG GTCCAGGTGC AGGCACTGAC 
     4861 GCAGGAGGGC CAGGGGGCCG GCAGCAAGGT GCACGAATTC CAGACGCTGT CCCCGGAGGG 
     4921 ATCTGGCAAC TTGGCGGTGA TTGGCGGCGT GGCTGTCGGT GTGGTCCTGC TTCTGGTGCT 
     4981 GGCAGGAGTT GGCTTCTTTA TCCACCGCAG GAGGAAGAAC CAGCGTGCCC GCCAGTCCCC 
     5041 GGAGGACGTT TACTTCTCCA AGTCAGAACA ACTGAAGCCC CTGAAGACAT ACGTGGACCC 
     5101 CCACACATAT GAGGACCCCA ACCAGGCTGT GTTGAAGTTC ACTACCGAGA TCCATCCATC 
     5161 CTGTGTCACT CGGCAGAAGG TGATCGGAGC AGGAGAGTTT GGGGAGGTGT ACAAGGGCAT 
     5221 GCTGAAGACA TCCTCGGGGA AGAAGGAGGT GCCGGTGGCC ATCAAGACGC TGAAAGCCGG 
     5281 CTACACAGAG AAGCAGCGAG TGGACTTCCT CGGCGAGGCC GGCATCATGG GCCAGTTCAG 
     5341 CCACCACAAC ATCATCCGCC TAGAGGGCGT CATCTCCAAA TACAAGCCCA TGATGATCAT 
     5401 CACTGAGTAC ATGGAGAATG GGGCCCTGGA CAAGTTCCTT CGGGAGAAGG ATGGCGAGTT 
     5461 CAGCGTGCTG CAGCTGGTGG GCATGCTGCG GGGCATCGCA GCTGGCATGA AGTACCTGGC 
     5521 CAACATGAAC TATGTGCACC GTGACCTGGC TGCCCGCAAC ATCCTCGTCA ACAGCAACCT 
     5581 GGTCTGCAAG GTGTCTGACT TTGGCCTGTC CCGCGTGCTG GAGGACGACC CCGAGGCCAC 
     5641 CTACACCACC AGTGGCGGCA AGATCCCCAT CCGCTGGACC GCCCCGGAGG CCATTTCCTA 
     5701 CCGGAAGTTC ACCTCTGCCA GCGACGTGTG GAGCTTTGGC ATTGTCATGT GGGAGGTGAT 
     5761 GACCTATGGC GAGCGGCCCT ACTGGGAGTT GTCCAACCAC GAGGTGATGA AAGCCATCAA 
     5821 TGATGGCTTC CGGCTCCCCA CACCCATGGA CTGCCCCTCC GCCATCTACC AGCTCATGAT 
     5881 GCAGTGCTGG CAGCAGGAGC GTGCCCGCCG CCCCAAGTTC GCTGACATCG TCAGCATCCT 
     5941 GGACAAGCTC ATTCGTGCCC CTGACTCCCT CAAGACCCTG GCTGACTTTG ACCCCCGCGT 
     6001 GTCTATCCGG CTCCCCAGCA CGAGCGGCTC GGAGGGGGTG CCCTTCCGCA CGGTGTCCGA 
     6061 GTGGCTGGAG TCCATCAAGA TGCAGCAGTA TACGGAGCAC TTCATGGCGG CCGGCTACAC 
     6121 TGCCATCGAG AAGGTGGTGC AGATGACCAA CGACGACATC AAGAGGATTG GGGTGCGGCT 
     6181 GCCCGGCCAC CAGAAGCGCA TCGCCTACAG CCTGCTGGGA CTCAAGGACC AGGTGAACAC 
     6241 TGTGGGGATC CCCATCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     6301 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     6361 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     6421 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     6481 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6541 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6601 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6661 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6721 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6781 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     6841 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     6901 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     6961 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     7021 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     7081 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     7141 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     7201 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     7261 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     7321 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     7381 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     7441 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     7501 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7561 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7621 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7681 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7741 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7801 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     7861 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     7921 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     7981 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     8041 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     8101 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     8161 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     8221 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     8281 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     8341 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     8401 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     8461 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     8521 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8581 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8641 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8701 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8761 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     8821 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     8881 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     8941 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     9001 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     9061 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     9121 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     9181 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     9241 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     9301 GAC
//