LOCUS       RC205725                7809 bp    DNA     circular  SYN 
DEFINITION  EPHA2 (Myc-DDK-tagged)-Human EPH receptor A2 (EPHA2)
ACCESSION   NM_004431
VERSION     NM_004431.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3966..4057 
                     /label="Myc-DDK"  
     misc_feature    4087..4672 
                     /label="PolyA signal"  
     misc_feature    4144..4163 
                     /label="Restriction Enzyme"  
     misc_feature    4948..5620 
                     /label="ColE1"  
     misc_feature    6050..6844 
                     /label="Kan/Neo"  
     misc_feature    6879..7181 
                     /label="SV40 ori"  
     misc_feature    7267..7744 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3948..3954 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3948 
                     /label="EPHA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCCAG GCAGCCCGCG CCTGCTTCGC CCTGCTGTGG GGCTGTGCGC 
     1081 TGGCCGCGGC CGCGGCGGCG CAGGGCAAGG AAGTGGTACT GCTGGACTTT GCTGCAGCTG 
     1141 GAGGGGAGCT CGGCTGGCTC ACACACCCGT ATGGCAAAGG GTGGGACCTG ATGCAGAACA 
     1201 TCATGAATGA CATGCCGATC TACATGTACT CCGTGTGCAA CGTGATGTCT GGCGACCAGG 
     1261 ACAACTGGCT CCGCACCAAC TGGGTGTACC GAGGAGAGGC TGAGCGTATC TTCATTGAGC 
     1321 TCAAGTTTAC TGTACGTGAC TGCAACAGCT TCCCTGGTGG CGCCAGCTCC TGCAAGGAGA 
     1381 CTTTCAACCT CTACTATGCC GAGTCGGACC TGGACTACGG CACCAACTTC CAGAAGCGCC 
     1441 TGTTCACCAA GATTGACACC ATTGCGCCCG ATGAGATCAC CGTCAGCAGC GACTTCGAGG 
     1501 CACGCCACGT GAAGCTGAAC GTGGAGGAGC GCTCCGTGGG GCCGCTCACC CGCAAAGGCT 
     1561 TCTACCTGGC CTTCCAGGAT ATCGGTGCCT GTGTGGCGCT GCTCTCCGTC CGTGTCTACT 
     1621 ACAAGAAGTG CCCCGAGCTG CTGCAGGGCC TGGCCCACTT CCCTGAGACC ATCGCCGGCT 
     1681 CTGATGCACC TTCCCTGGCC ACTGTGGCCG GCACCTGTGT GGACCATGCC GTGGTGCCAC 
     1741 CGGGGGGTGA AGAGCCCCGT ATGCACTGTG CAGTGGATGG CGAGTGGCTG GTGCCCATTG 
     1801 GGCAGTGCCT GTGCCAGGCA GGCTACGAGA AGGTGGAGGA TGCCTGCCAG GCCTGCTCGC 
     1861 CTGGATTTTT TAAGTTTGAG GCATCTGAGA GCCCCTGCTT GGAGTGCCCT GAGCACACGC 
     1921 TGCCATCCCC TGAGGGTGCC ACCTCCTGCG AGTGTGAGGA AGGCTTCTTC CGGGCACCTC 
     1981 AGGACCCAGC GTCGATGCCT TGCACACGAC CCCCCTCCGC CCCACACTAC CTCACAGCCG 
     2041 TGGGCATGGG TGCCAAGGTG GAGCTGCGCT GGACGCCCCC TCAGGACAGC GGGGGCCGCG 
     2101 AGGACATTGT CTACAGCGTC ACCTGCGAAC AGTGCTGGCC CGAGTCTGGG GAATGCGGGC 
     2161 CGTGTGAGGC CAGTGTGCGC TACTCGGAGC CTCCTCACGG ACTGACCCGC ACCAGTGTGA 
     2221 CAGTGAGCGA CCTGGAGCCC CACATGAACT ACACCTTCAC CGTGGAGGCC CGCAATGGCG 
     2281 TCTCAGGCCT GGTAACCAGC CGCAGCTTCC GTACTGCCAG TGTCAGCATC AACCAGACAG 
     2341 AGCCCCCCAA GGTGAGGCTG GAGGGCCGCA GCACCACCTC GCTTAGCGTC TCCTGGAGCA 
     2401 TCCCCCCGCC GCAGCAGAGC CGAGTGTGGA AGTACGAGGT CACTTACCGC AAGAAGGGAG 
     2461 ACTCCAACAG CTACAATGTG CGCCGCACCG AGGGTTTCTC CGTGACCCTG GACGACCTGG 
     2521 CCCCAGACAC CACCTACCTG GTCCAGGTGC AGGCACTGAC GCAGGAGGGC CAGGGGGCCG 
     2581 GCAGCAAGGT GCACGAATTC CAGACGCTGT CCCCGGAGGG ATCTGGCAAC TTGGCGGTGA 
     2641 TTGGCGGCGT GGCTGTCGGT GTGGTCCTGC TTCTGGTGCT GGCAGGAGTT GGCTTCTTTA 
     2701 TCCACCGCAG GAGGAAGAAC CAGCGTGCCC GCCAGTCCCC GGAGGACGTT TACTTCTCCA 
     2761 AGTCAGAACA ACTGAAGCCC CTGAAGACAT ACGTGGACCC CCACACATAT GAGGACCCCA 
     2821 ACCAGGCTGT GTTGAAGTTC ACTACCGAGA TCCATCCATC CTGTGTCACT CGGCAGAAGG 
     2881 TGATCGGAGC AGGAGAGTTT GGGGAGGTGT ACAAGGGCAT GCTGAAGACA TCCTCGGGGA 
     2941 AGAAGGAGGT GCCGGTGGCC ATCAAGACGC TGAAAGCCGG CTACACAGAG AAGCAGCGAG 
     3001 TGGACTTCCT CGGCGAGGCC GGCATCATGG GCCAGTTCAG CCACCACAAC ATCATCCGCC 
     3061 TAGAGGGCGT CATCTCCAAA TACAAGCCCA TGATGATCAT CACTGAGTAC ATGGAGAATG 
     3121 GGGCCCTGGA CAAGTTCCTT CGGGAGAAGG ATGGCGAGTT CAGCGTGCTG CAGCTGGTGG 
     3181 GCATGCTGCG GGGCATCGCA GCTGGCATGA AGTACCTGGC CAACATGAAC TATGTGCACC 
     3241 GTGACCTGGC TGCCCGCAAC ATCCTCGTCA ACAGCAACCT GGTCTGCAAG GTGTCTGACT 
     3301 TTGGCCTGTC CCGCGTGCTG GAGGACGACC CCGAGGCCAC CTACACCACC AGTGGCGGCA 
     3361 AGATCCCCAT CCGCTGGACC GCCCCGGAGG CCATTTCCTA CCGGAAGTTC ACCTCTGCCA 
     3421 GCGACGTGTG GAGCTTTGGC ATTGTCATGT GGGAGGTGAT GACCTATGGC GAGCGGCCCT 
     3481 ACTGGGAGTT GTCCAACCAC GAGGTGATGA AAGCCATCAA TGATGGCTTC CGGCTCCCCA 
     3541 CACCCATGGA CTGCCCCTCC GCCATCTACC AGCTCATGAT GCAGTGCTGG CAGCAGGAGC 
     3601 GTGCCCGCCG CCCCAAGTTC GCTGACATCG TCAGCATCCT GGACAAGCTC ATTCGTGCCC 
     3661 CTGACTCCCT CAAGACCCTG GCTGACTTTG ACCCCCGCGT GTCTATCCGG CTCCCCAGCA 
     3721 CGAGCGGCTC GGAGGGGGTG CCCTTCCGCA CGGTGTCCGA GTGGCTGGAG TCCATCAAGA 
     3781 TGCAGCAGTA TACGGAGCAC TTCATGGCGG CCGGCTACAC TGCCATCGAG AAGGTGGTGC 
     3841 AGATGACCAA CGACGACATC AAGAGGATTG GGGTGCGGCT GCCCGGCCAC CAGAAGCGCA 
     3901 TCGCCTACAG CCTGCTGGGA CTCAAGGACC AGGTGAACAC TGTGGGGATC CCCATCACGC 
     3961 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4021 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4081 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4141 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4201 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4261 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4321 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4381 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4441 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4501 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4561 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4621 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4681 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4741 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4801 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4861 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4921 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4981 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5041 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5101 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5161 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5221 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5281 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5341 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5401 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5461 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5521 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5581 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5641 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5701 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5761 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5821 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5881 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5941 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6001 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6061 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6121 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6181 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6241 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6301 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6361 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6421 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6481 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6541 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6601 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6661 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6721 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6781 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6841 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6901 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6961 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7021 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7081 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7141 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7201 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7261 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7321 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7381 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7441 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7501 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7561 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7621 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7681 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7741 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7801 GCGAATTTT
//