LOCUS       RC205678                6936 bp    DNA     circular  SYN 
DEFINITION  STIM1 (Myc-DDK-tagged)-Human stromal interaction molecule 1 (STIM1)
ACCESSION   NM_003156
VERSION     NM_003156.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3093..3184 
                     /label="Myc-DDK"  
     misc_feature    3214..3799 
                     /label="PolyA signal"  
     misc_feature    3271..3290 
                     /label="Restriction Enzyme"  
     misc_feature    4075..4747 
                     /label="ColE1"  
     misc_feature    5177..5971 
                     /label="Kan/Neo"  
     misc_feature    6006..6308 
                     /label="SV40 ori"  
     misc_feature    6394..6871 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3075..3081 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3075 
                     /label="STIM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTATGC GTCCGTCTTG CCCTGTGGCT CCTCTGGGGA CTCCTCCTGC 
     1081 ACCAGGGCCA GAGCCTCAGC CATAGTCACA GTGAGAAGGC GACAGGAACC AGCTCGGGGG 
     1141 CCAACTCTGA GGAGTCCACT GCAGCAGAGT TTTGCCGAAT TGACAAGCCC CTGTGTCACA 
     1201 GTGAGGATGA GAAACTCAGC TTCGAGGCAG TCCGTAACAT CCACAAACTG ATGGACGATG 
     1261 ATGCCAATGG TGATGTGGAT GTGGAAGAAA GTGATGAGTT CCTGAGGGAA GACCTCAATT 
     1321 ACCATGACCC AACAGTGAAA CACAGCACCT TCCATGGTGA GGATAAGCTC ATCAGCGTGG 
     1381 AGGACCTGTG GAAGGCATGG AAGTCATCAG AAGTATACAA TTGGACCGTG GATGAGGTGG 
     1441 TACAGTGGCT GATCACATAT GTGGAGCTGC CTCAGTATGA GGAGACCTTC CGGAAGCTGC 
     1501 AGCTCAGTGG CCATGCCATG CCAAGGCTGG CTGTCACCAA CACCACCATG ACAGGGACTG 
     1561 TGCTGAAGAT GACAGACCGG AGTCATCGGC AGAAGCTGCA GCTGAAGGCT CTGGATACAG 
     1621 TGCTCTTTGG GCCTCCTCTC TTGACTCGCC ATAATCACCT CAAGGACTTC ATGCTGGTGG 
     1681 TGTCTATCGT TATTGGTGTG GGCGGCTGCT GGTTTGCCTA TATCCAGAAC CGTTACTCCA 
     1741 AGGAGCACAT GAAGAAGATG ATGAAGGACT TGGAGGGGTT ACACCGAGCT GAGCAGAGTC 
     1801 TGCATGACCT TCAGGAAAGG CTGCACAAGG CCCAGGAGGA GCACCGCACA GTGGAGGTGG 
     1861 AGAAGGTCCA TCTGGAAAAG AAGCTGCGCG ATGAGATCAA CCTTGCTAAG CAGGAAGCCC 
     1921 AGCGGCTGAA GGAGCTGCGG GAGGGTACTG AGAATGAGCG GAGCCGCCAA AAATATGCTG 
     1981 AGGAGGAGTT GGAGCAGGTT CGGGAGGCCT TGAGGAAAGC AGAGAAGGAG CTAGAATCTC 
     2041 ACAGCTCATG GTATGCTCCA GAGGCCCTTC AGAAGTGGCT GCAGCTGACA CATGAGGTGG 
     2101 AGGTGCAATA TTACAACATC AAGAAGCAAA ATGCTGAGAA GCAGCTGCTG GTGGCCAAGG 
     2161 AGGGGGCTGA GAAGATAAAA AAGAAGAGAA ACACACTCTT TGGCACCTTC CACGTGGCCC 
     2221 ACAGCTCTTC CCTGGATGAT GTAGATCATA AAATTCTAAC AGCTAAGCAA GCACTGAGCG 
     2281 AGGTGACAGC AGCATTGCGG GAGCGCCTGC ACCGCTGGCA ACAGATCGAG ATCCTCTGTG 
     2341 GCTTCCAGAT TGTCAACAAC CCTGGCATCC ACTCACTGGT GGCTGCCCTC AACATAGACC 
     2401 CCAGCTGGAT GGGCAGTACA CGCCCCAACC CTGCTCACTT CATCATGACT GACGACGTGG 
     2461 ATGACATGGA TGAGGAGATT GTGTCTCCCT TGTCCATGCA GTCCCCTAGC CTGCAGAGCA 
     2521 GTGTTCGGCA GCGCCTGACG GAGCCACAGC ATGGCCTGGG ATCTCAGAGG GATTTGACCC 
     2581 ATTCCGATTC GGAGTCCTCC CTCCACATGA GTGACCGCCA GCGTGTGGCC CCCAAACCTC 
     2641 CTCAGATGAG CCGTGCTGCA GACGAGGCTC TCAATGCCAT GACTTCCAAT GGCAGCCACC 
     2701 GGCTGATCGA GGGGGTCCAC CCAGGGTCTC TGGTGGAGAA ACTGCCTGAC AGCCCTGCCC 
     2761 TGGCCAAGAA GGCATTACTG GCGCTGAACC ATGGGCTGGA CAAGGCCCAC AGCCTGATGG 
     2821 AGCTGAGCCC CTCAGCCCCA CCTGGTGGCT CTCCACATTT GGATTCTTCC CGTTCTCACA 
     2881 GCCCCAGCTC CCCAGACCCA GACACACCAT CTCCAGTTGG GGACAGCCGA GCCCTGCAAG 
     2941 CCAGCCGAAA CACACGCATT CCCCACCTGG CTGGCAAGAA GGCTGTGGCT GAGGAGGATA 
     3001 ATGGCTCTAT TGGCGAGGAA ACAGACTCCA GCCCAGGCCG GAAGAAGTTT CCCCTCAAAA 
     3061 TCTTTAAGAA GCCTCTTAAG AAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3121 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3181 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3241 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3301 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3361 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3421 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3481 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3541 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3601 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3661 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3721 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3781 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3841 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3901 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3961 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4021 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4081 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4141 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4201 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4261 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4321 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4381 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4441 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4501 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4561 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4621 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4681 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4741 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4801 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4861 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4921 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4981 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5041 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5101 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5161 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5221 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5281 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5341 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5401 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5461 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5521 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5581 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5641 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5701 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5761 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5821 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5881 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5941 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6001 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6061 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6121 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6181 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6241 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6301 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6361 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6421 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6481 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6541 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6601 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6661 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6721 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6781 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6841 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6901 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//