LOCUS       RC205553L2                9444 bp    DNA     circular  SYN 
DEFINITION  LRRC8B (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member B (LRRC8B), transcript variant 1
ACCESSION   NM_015350
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7735..7915 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8643..9302 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7963..8582 
                     /label="ColE1"  
     misc_feature    6610..6630 
                     /label="LR50"  
     misc_feature    5750..6475 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6619..7210 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5729..5735 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5729 
                     /label="LRRC8B"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GATTACACTA ACTGAGCTAA AATGCTTAGC 
     3361 AGATGCTCAG TCATCTTATC ACATCTTAAA ACCATGGTGG GACGTCTTCT GGTATTACAT 
     3421 CACACTGATC ATGCTGCTGG TGGCCGTGCT GGCCGGAGCT CTCCAGCTGA CGCAGAGCAG 
     3481 GGTTCTGTGC TGTCTTCCAT GCAAAGTGGA ATTTGACAAT CACTGTGCCG TGCCTTGGGA 
     3541 CATCCTGAAA GCCAGCATGA ACACATCCTC TAATCCTGGG ACACCGCTTC CGCTCCCCCT 
     3601 CCGAATTCAG AATGACCTCC ACCGACAGCA GTACTCCTAT ATTGATGCCG TCTGTTACGA 
     3661 GAAACAGCTC CATTGGTTTG CAAAGTTTTT CCCCTATCTG GTGCTCTTGC ACACGCTCAT 
     3721 CTTTGCAGCC TGCAGCAACT TTTGGCTTCA CTACCCCAGT ACCAGTTCCA GGCTCGAGCA 
     3781 TTTTGTGGCC ATCCTTCACA AGTGCTTCGA TTCTCCATGG ACCACCCGCG CCCTTTCAGA 
     3841 AACAGTGGCT GAGCAGTCAG TGAGGCCTCT GAAACTCTCC AAGTCCAAGA TTTTGCTTTC 
     3901 GTCCTCAGGG TGTTCAGCTG ACATAGATTC CGGCAAACAG TCATTGCCCT ACCCACAGCC 
     3961 AGGTTTGGAG TCAGCTGGCA TAGAAAGCCC AACTTCCAGT GTCCTGGACA AGAAGGAGGG 
     4021 TGAACAGGCC AAAGCCATCT TTGAAAAAGT GAAAAGATTC CGCATGCATG TGGAGCAGAA 
     4081 GGACATCATT TATAGAGTAT ATCTGAAACA GATAATAGTC AAAGTCATTT TGTTTGTGCT 
     4141 CATCATAACT TATGTTCCAT ATTTTTTAAC CCACATCACT CTTGAAATCG ACTGTTCAGT 
     4201 TGATGTGCAG GCTTTTACAG GATATAAGCG CTACCAGTGT GTCTATTCCT TGGCAGAAAT 
     4261 CTTTAAGGTC CTGGCTTCAT TTTATGTCAT TTTGGTTATA CTTTATGGTC TGACCTCTTC 
     4321 CTACAGCCTG TGGTGGATGC TGAGGAGTTC CCTGAAGCAA TATTCCTTTG AGGCGTTAAG 
     4381 AGAAAAAAGC AACTACAGTG ACATCCCTGA TGTCAAGAAT GACTTTGCCT TCATCCTTCA 
     4441 TCTGGCTGAT CAGTATGATC CTCTTTATTC CAAACGCTTC TCCATATTCC TATCAGAGGT 
     4501 CAGTGAGAAC AAACTGAAAC AGATCAACCT CAATAATGAA TGGACAGTTG AGAAACTGAA 
     4561 AAGTAAGCTT GTGAAAAATG CCCAGGACAA GATAGAACTG CATCTTTTTA TGCTCAACGG 
     4621 TCTTCCAGAC AATGTCTTTG AGTTAACTGA AATGGAAGTG CTAAGCCTGG AGCTTATCCC 
     4681 AGAGGTGAAG CTGCCCTCTG CAGTCTCACA GCTGGTCAAC CTCAAGGAGC TTCGTGTGTA 
     4741 CCATTCATCT CTGGTCGTAG ACCATCCTGC ACTGGCCTTT CTAGAGGAGA ATTTAAAAAT 
     4801 CCTCCGCCTG AAATTTACTG AAATGGGAAA AATCCCACGC TGGGTATTTC ACCTCAAGAA 
     4861 TCTCAAGGAA CTTTATCTTT CGGGCTGTGT TCTCCCTGAA CAGTTGAGTA CTATGCAGTT 
     4921 GGAGGGCTTT CAGGACTTAA AAAATCTAAG GACCCTGTAC TTGAAGAGCA GCCTCTCCCG 
     4981 GATCCCACAA GTTGTTACAG ACCTCCTGCC TTCATTGCAG AAACTGTCCC TTGATAATGA 
     5041 GGGAAGCAAA CTGGTTGTGT TGAACAACTT GAAAAAGATG GTCAATCTGA AAAGCCTAGA 
     5101 ACTGATCAGC TGTGACCTGG AACGCATCCC ACATTCCATT TTCAGCCTGA ATAATTTGCA 
     5161 TGAGTTAGAC CTAAGGGAAA ATAACCTTAA AACTGTGGAA GAGATCATTA GCTTTCAGCA 
     5221 TCTTCAGAAT CTTTCCTGCT TAAAGTTGTG GCACAATAAC ATTGCTTATA TTCCTGCACA 
     5281 GATTGGGGCA TTATCTAACC TAGAGCAGCT CTCTTTGGAC CATAATAATA TTGAGAATCT 
     5341 GCCCTTGCAG CTTTTCCTAT GCACTAAACT ACATTATTTG GATCTAAGCT ATAACCACTT 
     5401 GACCTTCATT CCAGAAGAAA TCCAGTATCT GAGTAATTTG CAGTACTTTG CTGTGACCAA 
     5461 CAACAATATT GAGATGCTAC CAGATGGGCT GTTTCAGTGC AAAAAGCTGC AGTGTTTACT 
     5521 TTTGGGGAAA AATAGCTTGA TGAATTTGTC CCCTCATGTG GGTGAGCTGT CAAACCTTAC 
     5581 TCATCTGGAG CTCATTGGTA ATTACCTGGA AACACTTCCT CCTGAACTAG AAGGATGTCA 
     5641 GTCCCTAAAA CGGAACTGTC TGATTGTTGA GGAGAACTTG CTCAATACTC TTCCTCTCCC 
     5701 TGTAACAGAA CGTTTACAGA CGTGCTTAGA CAAATGTACG CGTACGCGGC CGCTCGAGAT 
     5761 GAGCGGGGGC GAGGAGCTGT TCGCCGGCAT CGTGCCCGTG CTGATCGAGC TGGACGGCGA 
     5821 CGTGCACGGC CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGACGCCG ACTACGGCAA 
     5881 GCTGGAGATC AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTGGT 
     5941 GACCACCCTC TGCTACGGCA TCCAGTGCTT CGCCCGCTAC CCCGAGCACA TGAAGATGAA 
     6001 CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACATCCAG GAGCGCACCA TCCAGTTCCA 
     6061 GGACGACGGC AAGTACAAGA CCCGCGGCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA 
     6121 CCGCATCGAG CTGAAGGGCA AGGACTTCAA GGAGGACGGC AACATCCTGG GCCACAAGCT 
     6181 GGAGTACAGC TTCAACAGCC ACAACGTGTA CATCCGCCCC GACAAGGCCA ACAACGGCCT 
     6241 GGAGGCTAAC TTCAAGACCC GCCACAACAT CGAGGGCGGC GGCGTGCAGC TGGCCGACCA 
     6301 CTACCAGACC AACGTGCCCC TGGGCGACGG CCCCGTGCTG ATCCCCATCA ACCACTACCT 
     6361 GAGCACTCAG ACCAAGATCA GCAAGGACCG CAACGAGGCC CGCGACCACA TGGTGCTCCT 
     6421 GGAGTCCTTC AGCGCCTGCT GCCACACCCA CGGCATGGAC GAGCTGTACA GGTCCGGACT 
     6481 CAGATAAGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6541 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6601 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6661 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6721 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6781 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6841 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6901 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6961 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     7021 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     7081 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     7141 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     7201 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     7261 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     7321 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     7381 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7441 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7501 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7561 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7621 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7681 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7741 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7801 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7861 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7921 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7981 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     8041 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     8101 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     8161 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     8221 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     8281 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     8341 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     8401 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8461 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8521 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8581 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8641 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8701 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8761 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8821 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8881 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8941 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     9001 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     9061 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     9121 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     9181 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     9241 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     9301 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9361 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     9421 TTTCCCCGAA AAGTGCCACC TGAC
//