LOCUS       RC205550                8649 bp    DNA     circular  SYN 
DEFINITION  SSFA2 (Myc-DDK-tagged)-Human sperm specific antigen 2 (SSFA2), transcript variant 2
ACCESSION   NM_006751
VERSION     NM_006751.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="SSFA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCGGCCC CTGTCGTCGT CGGCGGAGGC GGAGGAGGAA CTGGAGTGGC 
     1081 AAGTGGCGAG TCGCAGGAGG AAGGCCTGGG CCAAGTGCCG CAGCTCCTGG CAAGCGTCGG 
     1141 AGACGGAGGA TCTGTCCACA GAAGCGACGA CGCAGGACGA GGAGGAGGAC GAGGAGGAGG 
     1201 ACCTCCCCGG CGCGCAGCTG CCGGCAGCGG GGGGAAGAGG AAACGTGCCC AACGAGAAGA 
     1261 TCGCGATATG GCTCAAGGAC TGCCGTACAC CTTTGGGAGC CTCACTGGAT GAACAAAGCA 
     1321 GTAGTACACT CAAGGGTGTG CTTGTGAGAA ATGGAGGAAG TTTTGAAGAT GATTTGTCAT 
     1381 TGGGAGCTGA AGCCAACCAC CTCCATGAAA GTGATGCTCA AATTGAAAAC TGCAATAATA 
     1441 TCTTGGCCAA AGAGAGAAGA TTACAGTTTC ATCAGAAAGG GAGAAGTATG AATTCCACTG 
     1501 GATCTGGGAA AAGTAGTGGG ACAGTTTCAA GTGTTTCAGA ATTGTTGGAA CTTTATGAGG 
     1561 AAGATCCTGA AGAAATTCTT TATAATCTTG GATTTGGACG TGATGAACCA GATATTGCTT 
     1621 CTAAAATTCC TTCCAGATTT TTTAATTCAT CATCCTTTGC CAAAGGGATA GATATTAAAG 
     1681 TATTTTTGAG TGCTCAGATG CAACGGATGG AAGTAGAAAA CCCAAATTAT GCTTTAACAA 
     1741 GCCGTTTTCG TCAAATTGAA GTGCTTACTA CTGTGGCCAA TGCGTTTTCT TCTTTATATT 
     1801 CTCAAGTCTC CGGGACGCCC CTGCAGAGAA TTGGAAGTAT GTCCTCAGTG ACCTCTAACA 
     1861 AGGAGACAGA CCCACCTCCA CCTTTAACTC GAAGTAACAC TGCAAATCGT TTAATGAAAA 
     1921 CACTCTCAAA ACTGAATTTA TGTGTTGATA AAACAGAGAA AGGAGAAAGT AGTAGTCCTT 
     1981 CTCCATCAGC TGAAAAAGGA AAGATTCTAA ATGTTTCAGT GATTGAAGAA AGTGGCAATA 
     2041 AAAACGATCA AAAGTCTCAA AAAATTATGA AGAAGAAAGA GTCATCTTCT ATGTTGGCTA 
     2101 CAGTTAAAGA AGAAGTCTCT GGTAGTTCAG CAGCTGTTAC GGAGAATGCT GATAGTGATA 
     2161 GAATTTCTGA TGAAGCAAAT AGTAATTTTA ACCAAGGAAC TGAAAATGAA CAAAGTAAAG 
     2221 AAACTCAAAG TCATGAGAGT AAACTGGGTG AGGAATCTGG TATTGTAGAA TCCAAATTAG 
     2281 ATAGTGATTT CAACATATCC AGCCACAGTG AGCTGGAAAA TAGCAGTGAG CTGAAAAGTG 
     2341 TCCATATATC CACACCTGAA AAAGAGCCTT GTGCACCACT GACAATACCA TCCATAAGAA 
     2401 ATATAATGAC ACAGCAGAAG GACTCCTTCG AAATGGAAGA GGTTCAAAGT ACGGAGGGAG 
     2461 AAGCTCCTCA TGTTCCAGCC ACTTACCAGC TAGGTCTTAC GAAGTCGAAA AGAGATCATC 
     2521 TGTTACGTAC TGCAAGTCAG CATTCCGATA GCAGTGGTTT TGCTGAAGAT TCTACAGACT 
     2581 GCCTATCCCT TAATCATCTT CAGGTTCAGG AGTCCTTGCA GGCTATGGGG AGTAGTGCTG 
     2641 ATAGTTGTGA CAGTGAGACA ACAGTTACGT CACTTGGTGA AGACCTTGCC ACACCAACAG 
     2701 CACAAGACCA GCCTTATTTT AATGAATCAG AGGAGGAGTC TCTTGTCCCT CTTCAGAAGG 
     2761 GACTAGAGAA GGCAGCAGCA GTTGCAGACA AAAGAAAATC AGGTAGCCAG GATTTCCCTC 
     2821 AGTGCAACAC CATTGAGAAT ACAGGAACTA AACAGTCCAC CTGTAGTCCA GGGGATCATA 
     2881 TCATTGAAAT TACTGAAGTG GAAGAGGATT TGTTTCCAGC AGAGACAGTA GAGCTACTGA 
     2941 GGGAAGCAAG TGCTGAAAGT GATGTGGGTA AAAGCAGTGA AAGTGAATTT ACTCAGTATA 
     3001 CCACACACCA TATTCTGAAA TCATTGGCTT CTATTGAAGC TAAATGCAGT GATATGAGCT 
     3061 CTGAAAATAC AACTGGGCCT CCCTCTTCCA TGGACAGAGT TAATACAGCT TTGCAAAGAG 
     3121 CTCAAATGAA GGTTTGCAGT CTGTCTAATC AAAGGATGGG GCGTAGCCTG CTAAAATCAA 
     3181 AAGATTTGTT AAAACAAAGG TACTTATTTG CAAAAGCTGG CTATCCTCTA AGAAGGTCTC 
     3241 AGTCTTTACC AACCACCTTA TTGAGCCCAG TAAGGGTTGT GTCCTCTGTC AATGTTCGAT 
     3301 TATCTCCAGG AAAAGAGACC AGATGCAGCC CACCTTCCTT CACCTATAAG TACACACCTG 
     3361 AAGAGGAGCA GGAATTGGAA AAGCGGGTGA TGGAACATGA TGGTCAGTCT TTAGTTAAAT 
     3421 CGACCATTTT CATCTCTCCA TCATCTGTGA AGAAAGAAGA AGCCCCCCAG AGTGAGGCGC 
     3481 CGCGGGTGGA GGAATGCCAT CATGGAAGGA CTCCTACCTG TTCACGGCTT GCTCCACCAC 
     3541 CAATGTCTCA GTCTACCTGT TCCCTTCATT CCATCCACTC TGAGTGGCAA GAAAGGCCCC 
     3601 TGTGTGAGCA CACAAGAACT CTGAGCACTC ACAGTGTTCC CAACATATCA GGGGCTACTT 
     3661 GTAGTGCCTT CGCTTCCCCT TTCGGGTGTC CTTACTCACA TAGACATGCC ACCTACCCTT 
     3721 ACCGAGTGTG CTCTGTGAAT CCTCCTTCAG CCATAGAAAT GCAGTTGCGA AGAGTATTAC 
     3781 ATGATATTAG AAACTCACTG CAGAATCTTT CACAGTACCC TATGATGAGA GGACCTGATC 
     3841 CTGCTGCTGC TCCATATAGT ACTCAGAAAT CATCTGTTCT ACCTCTTTAT GAAAATACTT 
     3901 TTCAGGAGCT CCAGGTAATG AGGCGGAGCC TGAATTTGTT TAGAACACAA ATGATGGATT 
     3961 TAGAATTGGC AATGCTGCGT CAGCAAACCA TGGTTTATCA TCATATGACT GAGGAGGAGA 
     4021 GGTTTGAAGT TGATCAGCTC CAGGGTTTGA GAAATTCAGT CCGAATGGAA CTTCAGGACC 
     4081 TGGAACTGCA GCTGGAGGAG CGCCTGCTGG GCCTGGAGGA ACAGCTTCGT GCTGTGCGCA 
     4141 TGCCTTCACC CTTCCGCTCC TCCGCACTCA TGGGAATGTG TGGCAGTAGA AGCGCTGATA 
     4201 ACTTGTCATG CCCTTCTCCA TTGAATGTAA TGGAACCAGT CACTGAACTG ATGCAGGAGC 
     4261 AGTCATACCT GAAGTCTGAA TTGGGCCTGG GACTTGGAGA AATGGGATTT GAAATTCCTC 
     4321 CTGGAGAAAG CTCAGAATCT GTTTTTTCCC AAGCAACATC AGAATCATCT TCTGTATGTT 
     4381 CTGGTCCCTC TCATGCTAAC AGAAGAACTG GAGTACCTTC TACTGCCTCA GTGGGCAAAT 
     4441 CCAAAACCCC ATTAGTGGCA AGGAAGAAAG TGTTCCGAGC ATCGGTGGCT CTAACGCCAA 
     4501 CAGCTCCTTC TAGAACAGGC TCTGTGCAGA CACCTCCAGA TTTGGAAAGT TCTGAGGAAG 
     4561 TTGATGCAGC TGAAGGAGCC CCAGAAGTTG TAGGACCTAA ATCTGAAGTG GAAGAAGGGC 
     4621 ATGGAAAACT CCCATCAATG CCAGCTGCTG AGGAAATGCA TAAAAATGTG GAGCAAGATG 
     4681 AGTTGCAGCA AGTCATACGG GAGGTTGGCA TGGATCCTAT TAGCTGTGTA ATACTGGAAT 
     4741 TATCAATGAT ATGCACTGGT GGAGGTGTTA TTTGTGCTTT AGAAGATACT TGCTGTACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//