LOCUS       RC205515L3                9416 bp    DNA     circular  SYN 
DEFINITION  ADAM32 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 32 (ADAM32)
ACCESSION   NM_145004
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7707..7887 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8615..9274 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7935..8554 
                     /label="ColE1"  
     misc_feature    5747..5770 
                     /label="DDK"  
     misc_feature    6582..6602 
                     /label="LR50"  
     misc_feature    5699..5770 
                     /label="MYC"  
     misc_feature    5780..5845 
                     /label="P2A"  
     misc_feature    5846..6445 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6591..7182 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5681..5687 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5681 
                     /label="ADAM32"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GTTCCGCCTC TGGTTGCTGC TGGCCGGGCT 
     3361 CTGCGGCCTC CTGGCGTCAA GACCCGGTTT TCAAAATTCA CTTCTACAGA TCGTAATTCC 
     3421 AGAGAAAATC CAAACAAATA CAAATGACAG TTCAGAAATA GAATATGAAC AAATATCCTA 
     3481 TATTATTCCA ATAGATGAGA AACTGTACAC TGTGCACCTT AAACAAAGAT ATTTTTTAGC 
     3541 AGATAATTTT ATGATCTATT TGTACAATCA AGGATCTATG AATACTTATT CTTCAGATAT 
     3601 TCAGACTCAA TGCTACTATC AAGGAAATAT TGAAGGATAT CCTGATTCCA TGGTCACACT 
     3661 CAGCACGTGC TCTGGACTAA GAGGAATACT GCAATTTGAA AATGTTTCTT ATGGAATTGA 
     3721 GCCTCTGGAA TCTGCAGTTG AATTTCAGCA TGTTCTTTAC AAATTAAAGA ATGAAGACAA 
     3781 TGATATTGCA ATTTTTATTG ACAGAAGCCT GAAAGAACAA CCAATGGATG ACAACATTTT 
     3841 TATAAGTGAA AAATCAGAAC CAGCTGTTCC AGATTTATTT CCTCTTTATC TAGAAATGCA 
     3901 TATTGTGGTG GACAAAACTT TGTATGATTA CTGGGGCTCT GATAGCATGA TAGTAACAAA 
     3961 TAAAGTCATC GAAATTGTTG GCCTTGCAAA TTCAATGTTC ACCCAATTTA AAGTTACTAT 
     4021 TGTGCTGTCA TCATTGGAGT TATGGTCAGA TGAAAATAAG ATTTCTACAG TTGGTGAGGC 
     4081 AGATGAATTA TTGCAAAAAT TTTTAGAATG GAAACAATCT TATCTTAACC TAAGGCCTCA 
     4141 TGATATTGCA TATCTACTAA TTTATATGGA TTATCCTCGT TATTTGGGAG CAGTGTTTCC 
     4201 TGGAACAATG TGTATTACTC GTTATTCTGC AGGAGTTGCA TTGTACCCCA AGGAGATAAC 
     4261 TCTGGAGGCA TTTGCAGTTA TTGTCACCCA GATGCTGGCA CTCAGTCTGG GAATATCATA 
     4321 TGACGACCCA AAGAAATGTC AATGTTCAGA ATCCACCTGT ATAATGAATC CAGAAGTTGT 
     4381 GCAATCCAAT GGTGTGAAGA CTTTTAGCAG TTGCAGTTTG AGGAGCTTTC AAAATTTCAT 
     4441 TTCAAATGTG GGTGTCAAAT GTCTTCAGAA TAAGCCACAA ATGCAAAAAA AATCTCCGAA 
     4501 ACCAGTCTGC GGCAATGGCA GATTGGAGGG AAATGAAATC TGTGATTGTG GTACTGAGGC 
     4561 TCAATGTGGA CCTGCAAGCT GTTGTGATTT TCGAACTTGT GTACTGAAAG ACGGAGCAAA 
     4621 ATGTTATAAA GGACTGTGCT GCAAAGACTG TCAAATTTTA CAATCAGGCG TTGAATGTAG 
     4681 GCCGAAAGCA CATCCTGAAT GTGACATCGC TGAAAATTGT AATGGAAGCT CACCAGAATG 
     4741 TGGTCCTGAC ATAACTTTAA TCAATGGACT TTCATGCAAA AATAATAAGT TTATTTGTTA 
     4801 TGACGGAGAC TGCCATGATC TCGATGCACG TTGTGAGAGT GTATTTGGAA AAGGTTCAAG 
     4861 AAATGCTCCA TTTGCCTGCT ATGAAGAAAT ACAATCTCAA TCAGACAGAT TTGGGAACTG 
     4921 TGGTAGGGAT AGAAATAACA AATATGTGTT CTGTGGATGG AGGAATCTTA TATGTGGAAG 
     4981 ATTAGTTTGT ACATACCCTA CTCGAAAGCC TTTCCATCAA GAAAATGGTG ATGTGATTTA 
     5041 TGCTTTCGTA CGAGATTCTG TATGCATAAC TGTAGACTAC AAATTGCCTC GAACAGTTCC 
     5101 AGATCCACTG GCTGTCAAAA ATGGCTCTCA GTGTGATATT GGGAGGGTTT GTGTAAATCG 
     5161 TGAATGTGTA GAATCAAGGA TAATTAAGGC TTCAGCACAT GTTTGTTCAC AACAGTGTTC 
     5221 TGGACATGGA GTGTGTGATT CCAGAAACAA GTGCCATTGT TCGCCAGGCT ATAAGCCTCC 
     5281 AAACTGCCAA ATACGTTCCA AAGGATTTTC CATATTTCCT GAGGAAGATA TGGGTTCAAT 
     5341 CATGGAAAGA GCATCTGGGA AGACTGAAAA CACCTGGCTT CTAGGTTTCC TCATTGCTCT 
     5401 TCCTATTCTC ATTGTAACAA CCGCAATAGT TTTGGCAAGG AAACAGTTGA AAAAGTGGTT 
     5461 CGCCAAGGAA GAGGAATTCC CAAGTAGCGA ATCTAAATCG GAAGGTAGCA CACAGACATA 
     5521 TGCCAGCCAA TCCAGCTCAG AAGGCAGCAC TCAGACATAT GCCAGCCAAA CCAGATCAGA 
     5581 AAGCAGCAGT CAAGCTGATA CTAGCAAATC CAAATCAGAA GATAGTGCTG AAGCATATAC 
     5641 TAGCAGATCC AAATCACAGG ACAGTACCCA AACACAAAGC AGTAGTAACA CGCGTACGCG 
     5701 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     5761 CAAGGATGAC GACGATAAGG TTTGGGTAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA 
     5821 GCAGGCTGGA GACGTGGAGG AGAACCCTGG ACCTATGACC GAGTACAAGC CCACGGTGCG 
     5881 CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA 
     5941 CTACCCCGCC ACGCGCCACA CCGTCGATCC GGATCGCCAC ATCGAGCGGG TCACCGAGCT 
     6001 GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA 
     6061 CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC 
     6121 CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT 
     6181 GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG 
     6241 CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA 
     6301 GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC 
     6361 CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG 
     6421 CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATGTACA CCCAAACGGC CGGCCGCGGT 
     6481 CTGTACAAGT AGGATTCGTC GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG 
     6541 TTATACATGT TTAAGGGTTC CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA 
     6601 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 
     6661 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 
     6721 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 
     6781 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 
     6841 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 
     6901 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 
     6961 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG 
     7021 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 
     7081 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 
     7141 GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC 
     7201 GACCTCGATC GAGACCTAGA AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC 
     7261 AATGCTGATT GTGCCTGGCT AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA 
     7321 CCTCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA 
     7381 AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT 
     7441 CTGTGGATCT ACCACACACA AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA 
     7501 GGGATCAGAT ATCCACTGAC CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG 
     7561 AAGGTAGAAG AAGCCAATGA AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT 
     7621 GGGATGGATG ACCCGGAGAG AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT 
     7681 CATCACATGG CCCGAGAGCT GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC 
     7741 TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG 
     7801 CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC 
     7861 CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA 
     7921 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 
     7981 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 
     8041 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 
     8101 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 
     8161 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 
     8221 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 
     8281 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 
     8341 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 
     8401 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 
     8461 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 
     8521 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 
     8581 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC 
     8641 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA 
     8701 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT 
     8761 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC 
     8821 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG 
     8881 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT 
     8941 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA 
     9001 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA 
     9061 TACGGAACTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA 
     9121 ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT 
     9181 GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT 
     9241 GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT 
     9301 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     9361 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//