LOCUS       RC205515                7242 bp    DNA     circular  SYN 
DEFINITION  ADAM32 (Myc-DDK-tagged)-Human ADAM metallopeptidase domain 32 (ADAM32)
ACCESSION   NM_145004
VERSION     NM_145004.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3399..3490 
                     /label="Myc-DDK"  
     misc_feature    3520..4105 
                     /label="PolyA signal"  
     misc_feature    3577..3596 
                     /label="Restriction Enzyme"  
     misc_feature    4381..5053 
                     /label="ColE1"  
     misc_feature    5483..6277 
                     /label="Kan/Neo"  
     misc_feature    6312..6614 
                     /label="SV40 ori"  
     misc_feature    6700..7177 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3381..3387 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3381 
                     /label="ADAM32"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCCGCCTC TGGTTGCTGC TGGCCGGGCT CTGCGGCCTC CTGGCGTCAA 
     1081 GACCCGGTTT TCAAAATTCA CTTCTACAGA TCGTAATTCC AGAGAAAATC CAAACAAATA 
     1141 CAAATGACAG TTCAGAAATA GAATATGAAC AAATATCCTA TATTATTCCA ATAGATGAGA 
     1201 AACTGTACAC TGTGCACCTT AAACAAAGAT ATTTTTTAGC AGATAATTTT ATGATCTATT 
     1261 TGTACAATCA AGGATCTATG AATACTTATT CTTCAGATAT TCAGACTCAA TGCTACTATC 
     1321 AAGGAAATAT TGAAGGATAT CCTGATTCCA TGGTCACACT CAGCACGTGC TCTGGACTAA 
     1381 GAGGAATACT GCAATTTGAA AATGTTTCTT ATGGAATTGA GCCTCTGGAA TCTGCAGTTG 
     1441 AATTTCAGCA TGTTCTTTAC AAATTAAAGA ATGAAGACAA TGATATTGCA ATTTTTATTG 
     1501 ACAGAAGCCT GAAAGAACAA CCAATGGATG ACAACATTTT TATAAGTGAA AAATCAGAAC 
     1561 CAGCTGTTCC AGATTTATTT CCTCTTTATC TAGAAATGCA TATTGTGGTG GACAAAACTT 
     1621 TGTATGATTA CTGGGGCTCT GATAGCATGA TAGTAACAAA TAAAGTCATC GAAATTGTTG 
     1681 GCCTTGCAAA TTCAATGTTC ACCCAATTTA AAGTTACTAT TGTGCTGTCA TCATTGGAGT 
     1741 TATGGTCAGA TGAAAATAAG ATTTCTACAG TTGGTGAGGC AGATGAATTA TTGCAAAAAT 
     1801 TTTTAGAATG GAAACAATCT TATCTTAACC TAAGGCCTCA TGATATTGCA TATCTACTAA 
     1861 TTTATATGGA TTATCCTCGT TATTTGGGAG CAGTGTTTCC TGGAACAATG TGTATTACTC 
     1921 GTTATTCTGC AGGAGTTGCA TTGTACCCCA AGGAGATAAC TCTGGAGGCA TTTGCAGTTA 
     1981 TTGTCACCCA GATGCTGGCA CTCAGTCTGG GAATATCATA TGACGACCCA AAGAAATGTC 
     2041 AATGTTCAGA ATCCACCTGT ATAATGAATC CAGAAGTTGT GCAATCCAAT GGTGTGAAGA 
     2101 CTTTTAGCAG TTGCAGTTTG AGGAGCTTTC AAAATTTCAT TTCAAATGTG GGTGTCAAAT 
     2161 GTCTTCAGAA TAAGCCACAA ATGCAAAAAA AATCTCCGAA ACCAGTCTGC GGCAATGGCA 
     2221 GATTGGAGGG AAATGAAATC TGTGATTGTG GTACTGAGGC TCAATGTGGA CCTGCAAGCT 
     2281 GTTGTGATTT TCGAACTTGT GTACTGAAAG ACGGAGCAAA ATGTTATAAA GGACTGTGCT 
     2341 GCAAAGACTG TCAAATTTTA CAATCAGGCG TTGAATGTAG GCCGAAAGCA CATCCTGAAT 
     2401 GTGACATCGC TGAAAATTGT AATGGAAGCT CACCAGAATG TGGTCCTGAC ATAACTTTAA 
     2461 TCAATGGACT TTCATGCAAA AATAATAAGT TTATTTGTTA TGACGGAGAC TGCCATGATC 
     2521 TCGATGCACG TTGTGAGAGT GTATTTGGAA AAGGTTCAAG AAATGCTCCA TTTGCCTGCT 
     2581 ATGAAGAAAT ACAATCTCAA TCAGACAGAT TTGGGAACTG TGGTAGGGAT AGAAATAACA 
     2641 AATATGTGTT CTGTGGATGG AGGAATCTTA TATGTGGAAG ATTAGTTTGT ACATACCCTA 
     2701 CTCGAAAGCC TTTCCATCAA GAAAATGGTG ATGTGATTTA TGCTTTCGTA CGAGATTCTG 
     2761 TATGCATAAC TGTAGACTAC AAATTGCCTC GAACAGTTCC AGATCCACTG GCTGTCAAAA 
     2821 ATGGCTCTCA GTGTGATATT GGGAGGGTTT GTGTAAATCG TGAATGTGTA GAATCAAGGA 
     2881 TAATTAAGGC TTCAGCACAT GTTTGTTCAC AACAGTGTTC TGGACATGGA GTGTGTGATT 
     2941 CCAGAAACAA GTGCCATTGT TCGCCAGGCT ATAAGCCTCC AAACTGCCAA ATACGTTCCA 
     3001 AAGGATTTTC CATATTTCCT GAGGAAGATA TGGGTTCAAT CATGGAAAGA GCATCTGGGA 
     3061 AGACTGAAAA CACCTGGCTT CTAGGTTTCC TCATTGCTCT TCCTATTCTC ATTGTAACAA 
     3121 CCGCAATAGT TTTGGCAAGG AAACAGTTGA AAAAGTGGTT CGCCAAGGAA GAGGAATTCC 
     3181 CAAGTAGCGA ATCTAAATCG GAAGGTAGCA CACAGACATA TGCCAGCCAA TCCAGCTCAG 
     3241 AAGGCAGCAC TCAGACATAT GCCAGCCAAA CCAGATCAGA AAGCAGCAGT CAAGCTGATA 
     3301 CTAGCAAATC CAAATCAGAA GATAGTGCTG AAGCATATAC TAGCAGATCC AAATCACAGG 
     3361 ACAGTACCCA AACACAAAGC AGTAGTAACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3421 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3481 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3541 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3601 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3661 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3721 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3781 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3841 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3901 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3961 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4021 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4081 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4141 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4201 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4261 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4321 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4381 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4441 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4501 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4561 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4621 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4681 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4741 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4801 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4861 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4921 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4981 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5041 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5101 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5161 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5221 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5281 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5341 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5401 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5461 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5521 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5581 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5641 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5701 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5761 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5821 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5881 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5941 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6001 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6061 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6121 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6181 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6241 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6301 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6361 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6421 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6481 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6541 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6601 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6661 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6721 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6781 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6841 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6901 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6961 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7021 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7081 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7141 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7201 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//