LOCUS       RC205488                8487 bp    DNA     circular  SYN 
DEFINITION  PPFIA1 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2
ACCESSION   NM_003626
VERSION     NM_003626.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4644..4735 
                     /label="Myc-DDK"  
     misc_feature    4765..5350 
                     /label="PolyA signal"  
     misc_feature    4822..4841 
                     /label="Restriction Enzyme"  
     misc_feature    5626..6298 
                     /label="ColE1"  
     misc_feature    6728..7522 
                     /label="Kan/Neo"  
     misc_feature    7557..7859 
                     /label="SV40 ori"  
     misc_feature    7945..8422 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4626..4632 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4626 
                     /label="PPFIA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGCGAG GTGATGCCGA CCATCAGCGA AGCAGAAGGC CCCCCTGGAG 
     1081 GAGGTGGAGG CCATGGTTCC GGCTCCCCTT CACAGCCAGA TGCAGATTCA CATTTTGAAC 
     1141 AGTTGATGGT CTCCATGCTA GAAGAAAGGG ACCGCCTTCT TGATACACTG AGAGAGACTC 
     1201 AAGAAACGCT GGCCTTAACC CAGGGGAAGT TACACGAGGT TGGTCATGAA AGAGATTCCT 
     1261 TGCAGAGACA GCTCAACACG GCACTTCCAC AGGAGTTCGC AGCACTTACT AAAGAACTCA 
     1321 ATGTATGCAG GGAACAGCTC CTTGAAAGGG AAGAAGAAAT TGCTGAACTG AAAGCAGAAA 
     1381 GGAATAACAC CAGGCTGCTG TTAGAGCATT TGGAATGCCT TGTCTCCAGG CATGAGCGGT 
     1441 CTCTTAGGAT GACCGTGGTG AAGAGACAAG CGCAGTCTCC AGCAGGCGTG TCCAGCGAAG 
     1501 TGGAAGTGCT GAAAGCACTG AAGTCCTTAT TTGAACACCA CAAAGCTCTG GATGAAAAGG 
     1561 TGAGAGAGCG ATTACGAGTA GCACTTGAAA GATGTAGTTT GTTAGAAGAG GAATTAGGTG 
     1621 CCACACACAA AGAGCTAATG ATTCTTAAAG AACAGAATAA TCAGAAAAAA ACTCTAACAG 
     1681 ATGGAGTGCT GGACATAAAC CATGAACAAG AAAATACACC AAGCACGAGT GGAAAGAGAT 
     1741 CTTCTGATGG TTCTTTAAGC CACGAGGAAG ACCTTGCTAA AGTAATTGAG CTCCAAGAAA 
     1801 TCATAAGTAA GCAGTCAAGG GAACAGAGCC AAATGAAAGA ACGCCTGGCT TCCCTTTCCA 
     1861 GTCATGTGAC AGAACTGGAA GAGGATCTGG ACACGGCTAG AAAAGATCTC ATCAAATCTG 
     1921 AAGAAATGAA CACAAAATTG CAACGAGATG TCCGTGAAGC CATGGCCCAA AAGGAAGATA 
     1981 TGGAAGAGAG AATCACTACT CTTGAAAAAC GCTACCTCGC TGCACAGCGT GAAGCCACAT 
     2041 CTGTGCATGA CCTCAATGAT AAACTTGAAA ATGAAATTGC AAATAAAGAT TCTATGCATC 
     2101 GACAGACTGA AGATAAAAAC CGCCAGTTAC AGGAGCGCTT GGAATTGGCA GAGCAAAAGC 
     2161 TGCAACAGAC ACTGAGGAAG GCAGAGACGC TCCCGGAGGT GGAGGCGGAG CTGGCCCAGA 
     2221 GGGTGGCAGC GCTTTCCAAG GCTGAAGAGA GACACGGCAA CATTGAAGAA AGGTTACGAC 
     2281 AGATGGAAGC ACAGTTGGAG GAGAAGAATC AAGAACTGCA GCGGGCAAGG CAAAGAGAAA 
     2341 AAATGAACGA AGAACATAAT AAACGTTTAT CAGACACTGT TGACAAGCTG CTTTCAGAAT 
     2401 CTAATGAGAG GCTTCAACTT CATCTTAAAG AGAGAATGGC TGCTTTGGAA GATAAGAACT 
     2461 CTCTTTTAAG AGAAGTTGAA AGTGCAAAAA AGCAGTTAGA AGAAACACAA CACGATAAGG 
     2521 ATCAGCTTGT CCTAAACATT GAAGCACTGA GGGCTGAACT AGACCACATG AGACTAAGAG 
     2581 GTGCTTCACT TCATCATGGC CGACCCCACT TGGGCAGTGT CCCAGATTTC AGGTTCCCCA 
     2641 TGGCAGACGG CCACACAGAC TCCTACAGCA CCAGTGCAGT GCTGCGGCGC CCACAGAAAG 
     2701 GCCGGCTGGC AGCCCTGCGA GATGAGCCTT CCAAGGTACA AACTCTTAAT GAGCAGGATT 
     2761 GGGAACGTGC CCAGCAAGCT AGTGTCTTGG CAAATGTAGC ACAAGCATTC GAGAGTGATG 
     2821 CTGACGTGTC TGATGGTGAA GATGACAGGG ACACTCTCCT CAGCTCAGTT GACCTGCTAT 
     2881 CGCCCAGCGG GCAGGCCGAC GCGCACACAC TAGCCATGAT GCTTCAGGAG CAGCTGGACG 
     2941 CCATCAACAA AGAGATCAGG TTGATTCAGG AAGAAAAAGA AAATACAGAG CAGCGGGCAG 
     3001 AGGAGATTGA AAGTCGAGTT GGCAGTGGAA GTCTAGACAA TCTTGGTCGT TTTAGATCAA 
     3061 TGAGCTCCAT TCCCCCCTAC CCTGCTTCCT CGCTTGCTAG CTCCTCCCCT CCGGGCAGTG 
     3121 GGCGCTCCAC CCCACGAAGG ATCCCTCACA GCCCAGCTCG GGAAGTGGAC AGACTGGGCG 
     3181 TCATGACCCT TTTGCCACCT TCCAGAGAAG AGGTACGAGA TGACAAGACA ACCATAAAGT 
     3241 GTGAAACCTC CCCGCCTTCC TCCCCGAGAG CCCTTCGGTT AGACCGGCTG CACAAAGGGG 
     3301 CGCTGCACAC CGTCAGCCAC GAGGACATCA GGGACATAAG GAACTCCACA GGCTCCCAGG 
     3361 ATGGTCCCGT GAGCAACCCC AGCAGTAGCA ACAGTAGCCA GGACTCGCTC CACAAAGCCC 
     3421 CAAAGAAGAA AGGCATTAAG TCCTCCATTG GCCGCTTGTT TGGCAAGAAA GAAAAGGGCC 
     3481 GACCTGGACA AACTGGCAAA GAAGCATTAG GACAAGCTGG TGTTTCCGAG ACGGATAACT 
     3541 CATCTCAGGA TGCCTTGGGA CTTAGCAAAT TGGGGGGACA GGCTGAAAAA AATCGTAAAC 
     3601 TTCAAAAAAA GCATGAATTG CTGGAGGAAG CCCGGAGACA AGGTTTACCT TTTGCCCAAT 
     3661 GGGACGGGCC AACGGTTGTG GTCTGGCTAG AGCTCTGGGT TGGGATGCCA GCCTGGTATG 
     3721 TGGCTGCCTG CCGAGCAAAC GTGAAAAGCG GGGCCATCAT GTCGGCCCTG TCCGACACAG 
     3781 AGATCCAGCG TGAGATTGGC ATCAGCAACC CCCTGCACAG GCTGAAGCTG AGGCTGGCCA 
     3841 TCCAGGAGAT CATGTCGCTG ACCAGCCCGT CTGCCCCGCC CACATCTAGA ACGACACTCG 
     3901 CCTATGGGGA CATGAACCAC GAGTGGATCG GCAACGAGTG GCTCCCCAGC CTGGGCCTCC 
     3961 CCCAGTACCG CAGCTACTTC ATGGAGTGCC TTGTAGACGC CAGGATGCTG GACCACTTGA 
     4021 CCAAGAAAGA CCTTCGAGGG CAGCTGAAAA TGGTCGACAG TTTTCACAGA AACAGTTTCC 
     4081 AGTGTGGAAT TATGTGCCTG AGAAGGTTAA ATTATGACCG GAAAGAACTG GAAAGAAAAA 
     4141 GAGAAGAAAG TCAGAGTGAA ATAAAAGACG TGCTTGTTTG GAGCAATGAT CGAGTGATTC 
     4201 GCTGGATCCT GTCAATTGGC CTTAAAGAAT ATGCAAACAA TCTTATAGAG AGTGGTGTTC 
     4261 ACGGAGCACT TCTGGCCTTA GATGAAACCT TCGACTTCAG TGCACTGGCA CTGCTGTTAC 
     4321 AGATCCCGAC GCAGAACACA CAGGCTCGTG CTGTCTTGGA AAGAGAATTT AACAACCTTT 
     4381 TGGTCATGGG GACTGATAGA AGGTTTGATG AAGATGATGA TAAAAGCTTT AGGAGAGCAC 
     4441 CTTCATGGAG AAAAAAGTTT AGACCAAAGG ACATTCGTGG CTTAGCTGCT GGGTCAGCAG 
     4501 AGACTCTCCC TGCAAACTTC CGGGTGACTT CTTCTATGTC TTCCCCCTCT ATGCAGCCAA 
     4561 AGAAGATGCA GATGGACGGC AATGTATCAG GAACACAGAG GTTGGATTCT GCTACAGTCA 
     4621 GGACTTACTC CTGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4681 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4741 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4801 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4861 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4921 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4981 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5041 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5101 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5161 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5221 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5281 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5341 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5401 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5461 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5521 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5581 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5641 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5701 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5761 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5821 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5881 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5941 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6001 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6061 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6121 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6181 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6241 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6301 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6361 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6421 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6481 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6541 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6601 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6661 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6721 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6781 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6841 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6901 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6961 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7021 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7081 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7141 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7201 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7261 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7321 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7381 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7441 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7501 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7561 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7621 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7681 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7921 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7981 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8041 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8101 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8161 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8221 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8281 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8341 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8401 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8461 ATTTAACAAA AATTTAACGC GAATTTT
//