LOCUS       RC205473                8031 bp    DNA     circular  SYN 
DEFINITION  RASA1 (Myc-DDK-tagged)-Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1
ACCESSION   NM_002890
VERSION     NM_002890.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4186..4277 
                     /label="Myc-DDK"  
     misc_feature    4307..4892 
                     /label="PolyA signal"  
     misc_feature    4364..4383 
                     /label="Restriction Enzyme"  
     misc_feature    5168..5840 
                     /label="ColE1"  
     misc_feature    6270..7064 
                     /label="Kan/Neo"  
     misc_feature    7099..7401 
                     /label="SV40 ori"  
     misc_feature    7487..7964 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4161..4168 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4161 
                     /label="RASA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCGGCC GAGGCCGGCA GTGAGGAGGG CGGCCCGGTA ACAGCCGGAG 
     1081 CTGGAGGAGG CGGCGCGGCA GCGGGCTCCA GTGCCTATCC CGCAGTGTGT CGGGTGAAGA 
     1141 TACCCGCGGC CCTGCCTGTG GCAGCCGCCC CCTATCCTGG GCTGGTGGAG ACCGGAGTGG 
     1201 CTGGAACTCT GGGTGGCGGA GCCGCTTTGG GGTCAGAGTT CCTAGGAGCC GGGTCTGTGG 
     1261 CAGGGGCACT GGGGGGAGCT GGACTGACAG GGGGAGGTAC TGCTGCTGGC GTAGCTGGTG 
     1321 CTGCTGCTGG CGTGGCCGGT GCTGCTGTTG CTGGACCTAG TGGAGACATG GCTCTCACCA 
     1381 AACTGCCCAC TTCGTTGCTT GCTGAGACTC TCGGGCCAGG CGGCGGTTTT CCCCCTCTGC 
     1441 CCCCTCCCCC TTACCTGCCC CCTTTGGGGG CGGGCCTCGG GACAGTGGAC GAAGGTGACT 
     1501 CTCTGGATGG ACCAGAATAC GAGGAGGAAG AGGTGGCCAT ACCGTTGACC GCTCCTCCAA 
     1561 CTAACCAGTG GTATCACGGA AAACTTGACA GAACGATAGC AGAAGAACGC CTCAGGCAGG 
     1621 CAGGGAAGTC TGGCAGTTAT CTTATAAGAG AGAGTGATCG GAGGCCAGGG TCCTTTGTAC 
     1681 TTTCATTTCT TAGCCAGATG AATGTTGTCA ACCATTTTAG GATTATTGCT ATGTGTGGAG 
     1741 ATTACTACAT TGGTGGAAGA CGTTTTTCTT CACTGTCAGA CCTAATAGGT TATTACAGTC 
     1801 ATGTTTCTTG TTTGCTTAAA GGAGAAAAAT TACTTTACCC AGTTGCACCA CCAGAGCCAG 
     1861 TAGAAGATAG AAGGCGTGTA CGAGCTATTC TACCTTACAC AAAAGTACCA GACACTGATG 
     1921 AAATAAGTTT CTTAAAAGGA GATATGTTCA TTGTTCATAA TGAATTAGAA GATGGATGGA 
     1981 TGTGGGTTAC AAATTTAAGA ACAGATGAAC AAGGCCTTAT TGTTGAAGAC CTAGTAGAAG 
     2041 AGGTGGGCCG GGAAGAAGAT CCACATGAAG GAAAAATATG GTTCCATGGG AAGATTTCCA 
     2101 AACAGGAAGC TTATAATTTA CTAATGACAG TTGGTCAAGT CTGCAGTTTT CTTGTGAGGC 
     2161 CCTCAGATAA TACTCCTGGC GATTATTCAC TTTATTTCCG GACCAATGAA AATATTCAGC 
     2221 GATTTAAAAT ATGTCCAACG CCAAACAATC AGTTTATGAT GGGAGGCCGG TATTATAACA 
     2281 GCATTGGGGA CATCATAGAT CACTATCGAA AAGAACAGAT TGTTGAAGGA TATTATCTTA 
     2341 AGGAACCTGT ACCAATGCAG GATCAAGAAC AAGTACTCAA TGACACAGTG GATGGCAAGG 
     2401 AAATCTATAA TACCATCCGT CGTAAAACAA AGGATGCCTT TTATAAAAAC ATTGTTAAGA 
     2461 AAGGTTATCT TCTGAAAAAG GGCAAAGGAA AACGTTGGAA AAATTTATAT TTTATCTTAG 
     2521 AGGGTAGTGA TGCCCAACTT ATTTATTTTG AAAGCGAAAA ACGAGCTACC AAACCAAAAG 
     2581 GATTAATAGA TCTCAGTGTA TGTTCTGTCT ATGTCGTTCA TGATAGTCTC TTTGGCAGGC 
     2641 CAAACTGTTT TCAGATAGTA GTTCAGCACT TTAGTGAAGA ACATTACATC TTTTACTTTG 
     2701 CAGGAGAAAC TCCAGAACAA GCAGAGGATT GGATGAAAGG TCTGCAGGCA TTTTGCAATT 
     2761 TACGGAAAAG TAGTCCAGGG ACATCCAATA AACGCCTTCG TCAGGTCAGC AGCCTTGTTT 
     2821 TACATATTGA AGAAGCCCAT AAACTCCCAG TAAAACATTT TACTAATCCA TATTGTAACA 
     2881 TCTACCTGAA TAGTGTCCAA GTAGCAAAAA CTCATGCAAG GGAAGGGCAA AACCCAGTAT 
     2941 GGTCAGAAGA GTTTGTCTTT GATGATCTTC CTCCTGACAT CAATAGATTT GAAATAACTC 
     3001 TTAGTAATAA AACAAAGAAA AGCAAAGATC CTGATATCTT ATTTATGCGC TGCCAGTTGA 
     3061 GCCGATTACA GAAAGGGCAT GCCACAGATG AATGGTTTCT GCTCAGCTCC CATATACCAT 
     3121 TAAAAGGTAT TGAACCAGGG TCCCTGCGTG TTCGAGCACG ATACTCTATG GAAAAAATCA 
     3181 TGCCAGAAGA AGAGTACAGT GAATTTAAAG AGCTTATACT GCAAAAGGAA CTTCATGTAG 
     3241 TCTATGCTTT ATCACATGTA TGTGGACAAG ACCGAACACT ACTGGCCAGC ATCCTACTGA 
     3301 GGATTTTTCT TCACGAAAAG CTTGAATCGT TGTTGTTATG CACACTAAAT GACAGAGAAA 
     3361 TAAGCATGGA AGATGAAGCC ACTACCCTAT TTCGAGCCAC AACACTTGCA AGCACCTTGA 
     3421 TGGAGCAGTA TATGAAAGCC ACTGCTACAC AGTTTGTTCA TCATGCTTTG AAAGACTCTA 
     3481 TTTTAAAGAT AATGGAAAGC AAGCAGTCTT GTGAGTTAAG TCCATCAAAG TTAGAAAAAA 
     3541 ATGAAGATGT GAACACTAAT TTAACACACC TATTGAACAT ACTTTCAGAG CTTGTGGAGA 
     3601 AAATATTCAT GGCTTCAGAA ATACTTCCAC CGACATTGAG ATATATTTAT GGGTGTTTAC 
     3661 AGAAATCTGT TCAGCATAAG TGGCCTACAA ATACCACCAT GAGAACAAGA GTTGTTAGTG 
     3721 GTTTTGTTTT TCTTCGACTC ATCTGTCCTG CCATCCTGAA TCCACGGATG TTCAATATCA 
     3781 TCTCAGATTC TCCATCTCCT ATTGCTGCAA GAACACTGAT ATTAGTGGCT AAATCTGTGC 
     3841 AGAACTTAGC AAATCTTGTG GAATTTGGAG CTAAGGAGCC CTACATGGAA GGTGTCAATC 
     3901 CATTCATCAA AAGCAACAAA CATCGTATGA TCATGTTTTT AGATGAACTT GGGAATGTAC 
     3961 CTGAACTTCC GGACACTACA GAGCATTCTA GAACGGACCT GTCCCGTGAT TTAGCAGCAT 
     4021 TGCATGAGAT TTGCGTGGCT CATTCAGATG AACTTCGAAC GCTCAGTAAT GAGCGTGGTG 
     4081 CACAGCAGCA CGTATTGAAA AAGCTTCTGG CTATAACAGA ACTGCTTCAA CAAAAACAAA 
     4141 ACCAGTATAC AAAAACCAAT GATGTCAGGA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     4201 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4261 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4321 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4381 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4441 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4501 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4561 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4621 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4681 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4741 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4801 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4861 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4921 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4981 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5041 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5101 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5161 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5221 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5281 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5341 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5401 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5461 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5521 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5581 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5641 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5701 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5761 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5821 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5881 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5941 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6001 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6061 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6121 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6181 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6241 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6301 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6361 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6421 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6481 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6541 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6601 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6661 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6721 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6781 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6841 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6901 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6961 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7021 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7081 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7141 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7201 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7261 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7321 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7381 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7441 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7501 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7561 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7621 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7681 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7741 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7801 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7861 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7921 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7981 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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