LOCUS       RC205357                6603 bp    DNA     circular  SYN 
DEFINITION  NEURL (Myc-DDK-tagged)-Human neuralized homolog (Drosophila) (NEURL)
ACCESSION   NM_004210
VERSION     NM_004210.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2760..2851 
                     /label="Myc-DDK"  
     misc_feature    2881..3466 
                     /label="PolyA signal"  
     misc_feature    2938..2957 
                     /label="Restriction Enzyme"  
     misc_feature    3742..4414 
                     /label="ColE1"  
     misc_feature    4844..5638 
                     /label="Kan/Neo"  
     misc_feature    5673..5975 
                     /label="SV40 ori"  
     misc_feature    6061..6538 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2742..2748 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2742 
                     /label="NEURL1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTAACAAC TTCTCCAGTA TCCCCTCGCT GCCCCGAGGA AACCCGAGCC 
     1081 GCGCGCCGCG GGGCCACCCC CAGAACCTCA AAGACTCTAT CGGGGGCCCC TTCCCCGTCA 
     1141 CTTCTCACCG ATGCCACCAC AAGCAGAAGC ACTGTCCGGC AGTGCTGCCC AGCGGGGGGC 
     1201 TCCCAGCCAC GCCGCTGCTC TTCCACCCGC ACACCAAGGG CTCCCAGATC CTCATGGACC 
     1261 TCAGCCACAA GGCTGTCAAG AGGCAGGCCA GCTTCTGCAA CGCCATCACC TTCAGCAACC 
     1321 GCCCGGTCCT CATCTACGAG CAAGTCAGGC TGAAGATCAC CAAGAAGCAG TGCTGCTGGA 
     1381 GCGGGGCCCT GCGGCTGGGC TTCACCAGCA AGGACCCGTC CCGCATCCAC CCTGACTCGC 
     1441 TGCCCAAGTA CGCCTGCCCC GACCTGGTGT CCCAGAGTGG CTTCTGGGCC AAGGCGCTGC 
     1501 CTGAGGAGTT TGCCAATGAG GGCAACATCA TCGCATTCTG GGTGGACAAG AAGGGCCGTG 
     1561 TCTTCCACCG CATCAACGAC TCGGCTGTTA TGCTGTTCTT CAGCGGGGTC CGCACGGCCG 
     1621 ACCCGCTCTG GGCCCTGGTG GACGTCTACG GCCTCACGCG GGGCGTCCAG CTGCTTGATA 
     1681 GCGAGCTGGT GCTCCCGGAC TGTCTGCGGC CGCGCTCCTT CACCGCCCTG CGGCGGCCGT 
     1741 CGCTGCGGCG CGAGGCGGAC GACGCGCGCC TCTCGGTGAG CCTATGCGAC CTCAACGTGC 
     1801 CGGGCGCGGA CGGCGACGAG GCCGCGCCGG CCGCCGGCTG CCCCATCCCG CAGAACTCAC 
     1861 TCAACTCGCA GCACAGCCGC GCGCTGCCGG CGCAGCTCGA CGGCGACCTG CGTTTCCACG 
     1921 CCCTGCGCGC CGGCGCGCAC GTCCGCATCC TCGACGAGCA GACGGTGGCG CGCGTGGAGC 
     1981 ACGGGCGCGA CGAGCGCGCG CTCGTCTTCA CCAGCCGGCC CGTGCGCGTG GCCGAGACCA 
     2041 TCTTCGTCAA GGTCACGCGC TCGGGTGGCG CGCGGCCCGG CGCGCTGTCG TTCGGCGTCA 
     2101 CCACGTGCGA CCCCGGCACG CTGCGGCCGG CCGACCTGCC TTTCAGCCCT GAGGCCCTGG 
     2161 TGGACCGCAA GGAATTCTGG GCCGTGTGCC GCGTGCCCGG GCCCCTGCAC AGCGGCGACA 
     2221 TCCTGGGCCT GGTGGTCAAC GCCGACGGCG AGCTGCACCT CAGCCACAAT GGCGCGGCCG 
     2281 CCGGCATGCA GCTGTGCGTG GACGCCTCGC AGCCGCTTTG GATGCTCTTC GGCCTGCACG 
     2341 GGACCATCAC GCAGATCCGC ATCCTCGGCT CCACTATCCT GGCCGAGCGG GGTATCCCAT 
     2401 CACTCCCCTG CTCCCCTGCC TCCACGCCAA CCTCGCCCAG TGCCCTGGGC AGCCGCCTGT 
     2461 CTGACCCCTT GCTCAGCACG TGCAGCTCTG GCCCTCTGGG TAGCTCTGCT GGTGGGACAG 
     2521 CCCCCAATTC GCCAGTGAGC CTGCCCGAGT CGCCAGTGAC CCCAGGTCTG GGCCAGTGGA 
     2581 GCGATGAGTG CACCATTTGC TATGAACACG CGGTGGACAC GGTCATCTAC ACATGTGGCC 
     2641 ACATGTGCCT CTGCTACGCC TGTGGCCTGC GCCTCAAGAA GGCTCTGCAC GCCTGCTGCC 
     2701 CCATCTGCCG CCGCCCCATC AAGGACATCA TCAAGACCTA CCGCAGCTCC ACGCGTACGC 
     2761 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     2821 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     2881 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     2941 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3001 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3061 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3121 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3181 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3241 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3301 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3361 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     3421 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     3481 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     3541 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     3601 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     3661 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     3721 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     3781 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     3841 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     3901 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     3961 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4021 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4081 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4141 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4201 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4261 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4321 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4381 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     4441 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     4501 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     4561 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     4621 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     4681 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     4741 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     4801 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     4861 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     4921 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     4981 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5041 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5101 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5161 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5221 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5281 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5341 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     5401 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     5461 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     5521 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     5581 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     5641 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     5701 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     5761 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     5821 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     5881 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     5941 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6001 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6061 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6121 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6181 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6241 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6301 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6361 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     6421 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     6481 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     6541 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     6601 TTT
//