LOCUS       RC205295                8451 bp    DNA     circular  SYN 
DEFINITION  TJP2 (Myc-DDK-tagged)-Human tight junction protein 2 (zona occludens 2) (TJP2), transcript variant 1
ACCESSION   NM_004817
VERSION     NM_004817.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4608..4699 
                     /label="Myc-DDK"  
     misc_feature    4729..5314 
                     /label="PolyA signal"  
     misc_feature    4786..4805 
                     /label="Restriction Enzyme"  
     misc_feature    5590..6262 
                     /label="ColE1"  
     misc_feature    6692..7486 
                     /label="Kan/Neo"  
     misc_feature    7521..7823 
                     /label="SV40 ori"  
     misc_feature    7909..8386 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4590..4596 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4590 
                     /label="TJP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTGCGA GGAGACCGCG GGTTTCCACC CCGGCGGGAG CTGTCAGGTT 
     1081 GGCTCCGCGC CCCAGGCATG GAAGAGCTGA TATGGGAACA GTACACTGTG ACCCTACAAA 
     1141 AGGATTCCAA AAGAGGATTT GGAATTGCAG TGTCCGGAGG CAGAGACAAC CCCCACTTTG 
     1201 AAAATGGAGA AACGTCAATT GTCATTTCTG ATGTGCTCCC GGGTGGGCCT GCTGATGGGC 
     1261 TGCTCCAAGA AAATGACAGA GTGGTCATGG TCAATGGCAC CCCCATGGAG GATGTGCTTC 
     1321 ATTCGTTTGC AGTTCAGCAG CTCAGAAAAA GTGGGAAGGT CGCTGCTATT GTGGTCAAGA 
     1381 GGCCCCGGAA GGTCCAGGTG GCCGCACTTC AGGCCAGCCC TCCCCTGGAT CAGGATGACC 
     1441 GGGCTTTTGA GGTGATGGAC GAGTTTGATG GCAGAAGTTT CCGGAGTGGC TACAGCGAGA 
     1501 GGAGCCGGCT GAACAGCCAT GGGGGGCGCA GCCGCAGCTG GGAGGACAGC CCGGAAAGGG 
     1561 GGCGTCCCCA TGAGCGGGCC CGGAGCCGGG AGCGGGACCT CAGCCGGGAC CGGAGCCGTG 
     1621 GCCGGAGCCT GGAGCGGGGC CTGGACCAAG ACCATGCGCG CACCCGAGAC CGCAGCCGTG 
     1681 GCCGGAGCCT GGAGCGGGGC CTGGACCACG ACTTTGGGCC ATCCCGGGAC CGGGACCGTG 
     1741 ACCGCAGCCG CGGCCGGAGC ATTGACCAGG ACTACGAGCG AGCCTATCAC CGGGCCTACG 
     1801 ACCCAGACTA CGAGCGGGCC TACAGCCCGG AGTACAGGCG CGGGGCCCGC CACGATGCCC 
     1861 GCTCTCGGGG ACCCCGAAGC CGCAGCCGCG AGCACCCGCA CTCACGGAGC CCCAGCCCCG 
     1921 AGCCTAGGGG GCGGCCGGGG CCCATCGGGG TCCTCCTGAT GAAAAGCAGA GCGAACGAAG 
     1981 AGTATGGTCT CCGGCTTGGG AGTCAGATCT TCGTAAAGGA AATGACCCGA ACGGGTCTGG 
     2041 CAACTAAAGA TGGCAACCTT CACGAAGGAG ACATAATTCT CAAGATCAAT GGGACTGTAA 
     2101 CTGAGAACAT GTCTTTAACG GATGCTCGAA AATTGATAGA AAAGTCAAGA GGAAAACTAC 
     2161 AGCTAGTGGT GTTGAGAGAC AGCCAGCAGA CCCTCATCAA CATCCCGTCA TTAAATGACA 
     2221 GTGACTCAGA AATAGAAGAT ATTTCAGAAA TAGAGTCAAA CCGATCATTT TCTCCAGAGG 
     2281 AGAGACGTCA TCAGTATTCT GATTATGATT ATCATTCCTC AAGTGAGAAG CTGAAGGAAA 
     2341 GGCCAAGTTC CAGAGAGGAC ACGCCGAGCA GATTGTCCAG GATGGGTGCG ACACCCACTC 
     2401 CCTTTAAGTC CACAGGGGAT ATTGCAGGCA CAGTTGTCCC AGAGACCAAC AAGGAACCCA 
     2461 GATACCAAGA GGACCCCCCA GCTCCTCAAC CAAAAGCAGC CCCGAGAACT TTTCTTCGTC 
     2521 CTAGTCCTGA AGATGAAGCA ATATATGGCC CTAATACCAA AATGGTAAGG TTCAAGAAGG 
     2581 GAGACAGCGT GGGCCTCCGG TTGGCTGGTG GCAATGATGT CGGGATATTT GTTGCTGGCA 
     2641 TTCAAGAAGG GACCTCGGCG GAGCAGGAGG GCCTTCAAGA AGGAGACCAG ATTCTGAAGG 
     2701 TGAACACACA GGATTTCAGA GGATTAGTGC GGGAGGATGC CGTTCTCTAC CTGTTAGAAA 
     2761 TCCCTAAAGG TGAAATGGTG ACCATTTTAG CTCAGAGCCG AGCTGATGTG TATAGAGACA 
     2821 TCCTGGCTTG TGGCAGAGGG GATTCGTTTT TTATAAGAAG CCACTTTGAA TGTGAGAAGG 
     2881 AAACTCCACA GAGCCTGGCC TTCACCAGAG GGGAGGTCTT CCGAGTGGTA GACACACTGT 
     2941 ATGACGGCAA GCTGGGCAAC TGGCTGGCTG TGAGGATTGG GAACGAGTTG GAGAAAGGCT 
     3001 TAATCCCCAA CAAGAGCAGA GCTGAACAAA TGGCCAGTGT TCAAAATGCC CAGAGAGACA 
     3061 ACGCTGGGGA CCGGGCAGAT TTCTGGAGAA TGCGTGGCCA GAGGTCTGGG GTGAAGAAGA 
     3121 ACCTGAGGAA AAGTCGGGAA GACCTCACAG CTGTTGTGTC TGTCAGCACC AAGTTCCCAG 
     3181 CTTATGAGAG GGTTTTGCTG CGAGAAGCTG GTTTCAAGAG ACCTGTGGTC TTATTCGGCC 
     3241 CCATAGCTGA TATAGCAATG GAAAAATTGG CTAATGAGTT ACCTGACTGG TTTCAAACTG 
     3301 CTAAAACGGA ACCAAAAGAT GCAGGATCTG AGAAATCCAC TGGAGTGGTC CGGTTAAATA 
     3361 CCGTGAGGCA AATTATTGAG CAGGATAAGC ATGCACTACT GGATGTGACT CCGAAAGCTG 
     3421 TGGACCTGTT GAATTACACC CAGTGGTTCC CAATTGTGAT TTTTTTCAAC CCAGACTCCA 
     3481 GACAAGGTGT CAAAACCATG AGACAAAGGT TAAATCCAAC GTCCAACAAA AGTTCTCGAA 
     3541 AGTTATTTGA TCAAGCCAAC AAGCTTAAAA AAACGTGTGC ACACCTTTTT ACAGCTACAA 
     3601 TCAACCTAAA TTCAGCCAAT GATAGCTGGT TTGGCAGCTT AAAGGACACT ATTCAGCATC 
     3661 AGCAAGGAGA AGCGGTTTGG GTCTCTGAAG GAAAGATGGA AGGGATGGAT GATGACCCCG 
     3721 AAGACCGCAT GTCCTACTTA ACCGCCATGG GCGCGGACTA TCTGAGTTGC GACAGCCGCC 
     3781 TCATCAGTGA CTTTGAAGAC ACGGACGGTG AAGGAGGCGC CTACACTGAC AATGAGCTGG 
     3841 ATGAGCCAGC CGAGGAGCCG CTGGTGTCGT CCATCACCCG CTCCTCGGAG CCGGTGCAGC 
     3901 ACGAGGAGAG CATAAGGAAA CCCAGCCCAG AGCCACGAGC TCAGATGAGG AGGGCTGCTA 
     3961 GCAGCGATCA ACTTAGGGAC AATAGCCCGC CCCCAGCATT CAAGCCAGAG CCGCCCAAGG 
     4021 CCAAAACCCA GAACAAAGAA GAATCCTATG ACTTCTCCAA ATCCTATGAA TATAAGTCAA 
     4081 ACCCCTCTGC CGTTGCTGGT AATGAAACTC CTGGGGCATC TACCAAAGGT TATCCTCCTC 
     4141 CTGTTGCAGC AAAACCTACC TTTGGGCGGT CTATACTGAA GCCCTCCACT CCCATCCCTC 
     4201 CTCAAGAGGG TGAGGAGGTG GGAGAGAGCA GTGAGGAGCA AGATAATGCT CCCAAATCAG 
     4261 TCCTGGGCAA AGTCAAAATA TTTGAGAAGA TGGATCACAA GGCCAGGTTA CAGAGAATGC 
     4321 AGGAGCTCCA GGAAGCACAG AATGCAAGGA TCGAAATTGC CCAGAAGCAT CCTGATATCT 
     4381 ATGCAGTTCC AATCAAAACG CACAAGCCAG ACCCTGGCAC GCCCCAGCAC ACGAGTTCCA 
     4441 GACCCCCTGA GCCACAGAAA GCTCCTTCCA GACCTTATCA GGATACCAGA GGAAGTTATG 
     4501 GCAGTGATGC CGAGGAGGAG GAGTACCGCC AGCAGCTGTC AGAACACTCC AAGCGCGGTT 
     4561 ACTATGGCCA GTCTGCCCGA TACCGGGACA CAGAATTAAC GCGTACGCGG CCGCTCGAGC 
     4621 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4681 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4741 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4801 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4861 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4921 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4981 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5041 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5101 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5161 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5221 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5281 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5341 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5401 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5461 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5521 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5581 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5641 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5701 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5761 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5821 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5881 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5941 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6001 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6061 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6121 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6181 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6241 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6301 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6361 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6421 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6481 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6541 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6601 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6661 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6721 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6781 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6841 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6901 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6961 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7021 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7081 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7141 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7201 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7261 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7321 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7381 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7441 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7501 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7561 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7621 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7681 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7861 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7921 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7981 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8041 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8101 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8161 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8221 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8281 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8341 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8401 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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