LOCUS       RC205292L2                8670 bp    DNA     circular  SYN 
DEFINITION  PDE1A (Myc-DDK-tagged)-Human phosphodiesterase 1A, calmodulin-dependent (PDE1A), transcript variant 1
ACCESSION   NM_005019
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6961..7141 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7869..8528 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7189..7808 
                     /label="ColE1"  
     misc_feature    5836..5856 
                     /label="LR50"  
     misc_feature    4976..5701 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    5845..6436 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4955..4961 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4955 
                     /label="PDE1A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGGTCTAGT GCCACAGAGA TTGAAGAATT 
     3361 GGAAAACACC ACTTTTAAGT ATCTTACAGG AGAACAGACT GAAAAAATGT GGCAGCGCCT 
     3421 GAAAGGAATA CTAAGATGCT TGGTGAAGCA GCTGGAAAGA GGTGATGTTA ACGTCGTCGA 
     3481 CTTAAAGAAG AATATTGAAT ATGCGGCATC TGTGCTGGAA GCAGTTTATA TCGATGAAAC 
     3541 AAGAAGACTT CTGGATACTG AAGATGAGCT CAGTGACATT CAGACTGACT CAGTCCCATC 
     3601 TGAAGTCCGG GACTGGTTGG CTTCTACCTT TACACGGAAA ATGGGGATGA CAAAAAAGAA 
     3661 ACCTGAGGAA AAACCAAAAT TTCGGAGCAT TGTGCATGCT GTTCAAGCTG GAATTTTTGT 
     3721 GGAAAGAATG TACCGAAAAA CATATCATAT GGTTGGTTTG GCATATCCAG CAGCTGTCAT 
     3781 CGTAACATTA AAGGATGTTG ATAAATGGTC TTTCGATGTA TTTGCCCTAA ATGAAGCAAG 
     3841 TGGAGAGCAT AGTCTGAAGT TTATGATTTA TGAACTGTTT ACCAGATATG ATCTTATCAA 
     3901 CCGTTTCAAG ATTCCTGTTT CTTGCCTAAT CACCTTTGCA GAAGCTTTAG AAGTTGGTTA 
     3961 CAGCAAGTAC AAAAATCCAT ATCACAATTT GATTCATGCA GCTGATGTCA CTCAAACTGT 
     4021 GCATTACATA ATGCTTCATA CAGGTATCAT GCACTGGCTC ACTGAACTGG AAATTTTAGC 
     4081 AATGGTCTTT GCTGCTGCCA TTCATGATTA TGAGCATACA GGGACAACAA ACAACTTTCA 
     4141 CATTCAGACA AGGTCAGATG TTGCCATTTT GTATAATGAT CGCTCTGTCC TTGAGAATCA 
     4201 CCACGTGAGT GCAGCTTATC GACTTATGCA AGAAGAAGAA ATGAATATCT TGATAAATTT 
     4261 ATCCAAAGAT GACTGGAGGG ATCTTCGGAA CCTAGTGATT GAAATGGTTT TATCTACAGA 
     4321 CATGTCAGGT CACTTCCAGC AAATTAAAAA TATAAGAAAC AGTTTGCAGC AGCCTGAAGG 
     4381 GATTGACAGA GCCAAAACCA TGTCCCTGAT TCTCCACGCA GCAGACATCA GCCACCCAGC 
     4441 CAAATCCTGG AAGCTGCATT ATCGGTGGAC CATGGCCCTA ATGGAGGAGT TTTTCCTGCA 
     4501 GGGAGATAAA GAAGCTGAAT TAGGGCTTCC ATTTTCCCCA CTTTGTGATC GGAAGTCAAC 
     4561 CATGGTGGCC CAGTCACAAA TAGGTTTCAT CGATTTCATA GTAGAGCCAA CATTTTCTCT 
     4621 TCTGACAGAC TCAACAGAGA AAATTGTTAT TCCTCTTATA GAGGAAGCCT CAAAAGCCGA 
     4681 AACTTCTTCC TATGTGGCAA GCAGCTCAAC CACCATTGTG GGGTTACACA TTGCTGATGC 
     4741 ACTAAGACGA TCAAATACAA AAGGCTCCAT GAGTGATGGG TCCTATTCCC CAGACTACTC 
     4801 CCTTGCAGCA GTGGACCTGA AGAGTTTCAA GAACAACCTG GTGGACATCA TTCAGCAGAA 
     4861 CAAAGAGAGG TGGAAAGAGT TAGCTGCACA AGGTGAATCT GATCTTCATA AGAACTCAGA 
     4921 AGACTTAGTA AATGCTGAAG AAAAACATGA TGAGACACAT TCAACGCGTA CGCGGCCGCT 
     4981 CGAGATGAGC GGGGGCGAGG AGCTGTTCGC CGGCATCGTG CCCGTGCTGA TCGAGCTGGA 
     5041 CGGCGACGTG CACGGCCACA AGTTCAGCGT GCGCGGCGAG GGCGAGGGCG ACGCCGACTA 
     5101 CGGCAAGCTG GAGATCAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC 
     5161 CCTGGTGACC ACCCTCTGCT ACGGCATCCA GTGCTTCGCC CGCTACCCCG AGCACATGAA 
     5221 GATGAACGAC TTCTTCAAGA GCGCCATGCC CGAGGGCTAC ATCCAGGAGC GCACCATCCA 
     5281 GTTCCAGGAC GACGGCAAGT ACAAGACCCG CGGCGAGGTG AAGTTCGAGG GCGACACCCT 
     5341 GGTGAACCGC ATCGAGCTGA AGGGCAAGGA CTTCAAGGAG GACGGCAACA TCCTGGGCCA 
     5401 CAAGCTGGAG TACAGCTTCA ACAGCCACAA CGTGTACATC CGCCCCGACA AGGCCAACAA 
     5461 CGGCCTGGAG GCTAACTTCA AGACCCGCCA CAACATCGAG GGCGGCGGCG TGCAGCTGGC 
     5521 CGACCACTAC CAGACCAACG TGCCCCTGGG CGACGGCCCC GTGCTGATCC CCATCAACCA 
     5581 CTACCTGAGC ACTCAGACCA AGATCAGCAA GGACCGCAAC GAGGCCCGCG ACCACATGGT 
     5641 GCTCCTGGAG TCCTTCAGCG CCTGCTGCCA CACCCACGGC ATGGACGAGC TGTACAGGTC 
     5701 CGGACTCAGA TAAGTTTAAA CGGCCGGCCG CGGTCTGTAC AAGTAGGATT CGTCGAGGGA 
     5761 CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAC ATGTTTAAGG GTTCCGGTTC 
     5821 CACTAGGTAC AATTCGATAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT 
     5881 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 
     5941 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 
     6001 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 
     6061 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 
     6121 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 
     6181 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 
     6241 TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 
     6301 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 
     6361 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 
     6421 TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCTC GATCGAGACC TAGAAAAACA 
     6481 TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC 
     6541 ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC 
     6601 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT 
     6661 AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA 
     6721 CTTCCCTGAT TGGCAGAACT ACACACCAGG GCCAGGGATC AGATATCCAC TGACCTTTGG 
     6781 ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA 
     6841 GAACACCCGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT 
     6901 ATTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC ATGGCCCGAG AGCTGCATCC 
     6961 GGACTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT 
     7021 AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC 
     7081 CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA 
     7141 AATCTCTAGC AGCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     7201 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     7261 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     7321 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     7381 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     7441 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     7501 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     7561 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     7621 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     7681 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     7741 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     7801 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     7861 AGGGATTTTG GTCATGATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA 
     7921 TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC 
     7981 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG 
     8041 AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT 
     8101 GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA 
     8161 CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC 
     8221 CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA 
     8281 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ACTCCGGATG AGCATTCATC 
     8341 AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC 
     8401 TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC 
     8461 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA 
     8521 GTGATTTTTT TCTCCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8581 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8641 CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//