LOCUS       RC205228L3                9179 bp    DNA     circular  SYN 
DEFINITION  KLHL11 (Myc-DDK-tagged)-Human kelch-like 11 (Drosophila) (KLHL11)
ACCESSION   NM_018143
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7470..7650 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8378..9037 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7698..8317 
                     /label="ColE1"  
     misc_feature    5510..5533 
                     /label="DDK"  
     misc_feature    6345..6365 
                     /label="LR50"  
     misc_feature    5462..5533 
                     /label="MYC"  
     misc_feature    5543..5608 
                     /label="P2A"  
     misc_feature    5609..6208 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6354..6945 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5444..5450 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5444 
                     /label="KLHL11"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGCTGCG GCAGTGGCGG CGGCGGCGGC 
     3361 GGCGGCCGCG GCTGCATCTC TTCAGGTACT GGAGATGGAG AGCATGGAGA CGGCCGCCGC 
     3421 CGGCTCGGCA GGACTGGCCG CCGAGGTCCG AGGCAGCGGC ACGGTGGACT TCGGGCCTGG 
     3481 GCCGGGGATC TCTGCAATGG AGGCGAGCGG GGGCGATCCG GGCCCAGAAG CCGAGGATTT 
     3541 CGAGTGCAGC TCTCACTGCT CAGAGCTGTC CTGGCGGCAG AACGAGCAGC GGCGCCAGGG 
     3601 CCTCTTCTGC GACATTACCC TGTGCTTCGG CGGGGCTGGA GGCCGCGAGT TCCGGGCCCA 
     3661 CCGCTCGGTA CTGGCTGCCG CCACCGAGTA CTTCACGCCC CTGCTCTCGG GCCAGTTTTC 
     3721 CGAGTCCCGC TCGGGACGGG TGGAGATGCG CAAGTGGAGC TCCGAGCCGG GGCCCGAACC 
     3781 CGACACAGTG GAAGCCGTAA TCGAGTACAT GTACACCGGG CGCATCCGCG TCAGCACGGG 
     3841 CAGCGTGCAC GAGGTGCTGG AGTTGGCCGA CAGGTTCCTA CTCATTCGTT TAAAAGAATT 
     3901 TTGTGGAGAA TTTCTCAAGA AAAAACTTCA TCTCTCAAAT TGTGTGGCAA TTCATAGCTT 
     3961 AGCACACATG TACACCCTGA GCCAACTTGC TCTGAAGGCT GCTGATATGA TACGGAGAAA 
     4021 TTTCCACAAA GTGATTCAGG ATGAAGAATT TTATACGTTA CCTTTCCATC TCATTAGAGA 
     4081 CTGGCTTTCA GATTTGGAAA TTACTGTTGA TTCTGAAGAG GTTCTCTTTG AAACCGTTTT 
     4141 GAAATGGGTT CAGAGAAATG CTGAAGAGAG AGAGAGATAC TTTGAAGAAC TTTTTAAATT 
     4201 GCTCAGGTTG TCCCAGATGA AACCTACCTA CCTTACTCGA CATGTCAAAC CAGAGAGGCT 
     4261 GGTAGCCAAT AATGAAGTTT GTGTCAAGTT GGTCGCTGAC GCAGTGGAGA GACATGCTCT 
     4321 GAGAGCTGAG AATATACAAT CTGGCACATG CCAGCACCCC ACTTCTCATG TGTCACTATT 
     4381 GCCTCGTTAT GGGCAAAACA TGGATGTGAT CATGGTTATT GGAGGTGTGT CAGAAGGAGG 
     4441 GGACTATTTA AGTGAATGTG TGGGATATTT TGTTGATGAG GACAGATGGG TAAATCTGCC 
     4501 ACATATTCAT AATCACCTCG ATGGACATGC TGTTGCAGTA ACAGAATCCT ACGTGTATGT 
     4561 TGCTGGATCA ATGGAGCCAG GGTTTGCTAA AACTGTAGAA AGGTATAACC CAAATTTGAA 
     4621 TACATGGGAA CATGTTTGTA GTCTGATGAC AAGAAAGCAT TCTTTTGGAC TAACAGAAGT 
     4681 CAAAGGGAAG CTCTATAGCA TTGGAGGACA CGGCAACTTT AGTCCTGGTT TTAAAGATGT 
     4741 GACTGTTTAT AATCCTGAGC TTGATAAATG GCACAACTTG GAATCGGCAC CAAAGATTCT 
     4801 TCGAGATGTC AAAGCACTAG CCATTGAAGA CCGGTTTGTA TACATTGCCG CCCGCACTCC 
     4861 TGTAGACCGG GACACTGAAG ATGGATTAAA GGCTGTAATT ACTTGCTATG ATACAGAGAC 
     4921 TCGACAGTGG CAAGATGTGG AATCTTTGCC GCTTATTGAC AATTACTGCT TTTTCCAAAT 
     4981 GTCTGTGGTC AATTCAAACT TTTATCAGAC AGCATCATGT TGTCCCAAGA GTTATTGTTT 
     5041 AGAAAACGAA GAGGCAGTAA GAAAAATTGC CAGCCAAGTG TCTGATGAGA TCCTTGAAAG 
     5101 CTTGCCTCCA GAAGTCCTAA GCATCGAAGG AGCAGCCATT TGCTATTACA AAGATGATGT 
     5161 CTTCATTATA GGAGGCTGGA AAAACAGTGA TGATATTGAT AAACAGTATC GGAAAGAAGC 
     5221 CTACCGATAT TGTGCGGAGA GGAAGAGGTG GATGCTTCTT CCTCCTATGC CACAACCTCG 
     5281 TTGTAGAGCC ACTGCTTGTC ACGTGAGGAT CCCGTACCGG TACTTGCATG GCACACAGAG 
     5341 ATACCCTATG CCTCAAAACC TGATGTGGCA GAAGGACCGC ATCAGACAGA TGCAAGAGAT 
     5401 ACATCGTCAC GCCCTGAACA TGAGGCGAGT GCCAAGCTCT CAGATTGAAT GCACGCGTAC 
     5461 GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA 
     5521 TTACAAGGAT GACGACGATA AGGTTTGGGT AGGAAGCGGA GCTACTAACT TCAGCCTGCT 
     5581 GAAGCAGGCT GGAGACGTGG AGGAGAACCC TGGACCTATG ACCGAGTACA AGCCCACGGT 
     5641 GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC 
     5701 CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGATCGC CACATCGAGC GGGTCACCGA 
     5761 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA 
     5821 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT 
     5881 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA 
     5941 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT 
     6001 CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT 
     6061 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT 
     6121 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC 
     6181 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGATGT ACACCCAAAC GGCCGGCCGC 
     6241 GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG 
     6301 AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG 
     6361 ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 
     6421 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 
     6481 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 
     6541 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 
     6601 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 
     6661 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 
     6721 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC 
     6781 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 
     6841 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 
     6901 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC 
     6961 GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT 
     7021 ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC 
     7081 ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT 
     7141 TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT 
     7201 GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG 
     7261 CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA 
     7321 GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG 
     7381 CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA 
     7441 TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG GTTAGACCAG 
     7501 ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC 
     7561 TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA 
     7621 TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC AAAAGGCCAG 
     7681 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 
     7741 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 
     7801 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 
     7861 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 
     7921 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 
     7981 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 
     8041 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 
     8101 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 
     8161 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 
     8221 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 
     8281 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 
     8341 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC GCCCCGCCCT 
     8401 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC 
     8461 AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT 
     8521 ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA 
     8581 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT 
     8641 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA 
     8701 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT 
     8761 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG 
     8821 CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT 
     8881 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG 
     8941 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC 
     9001 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC TTCCTTTTTC 
     9061 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     9121 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC
//