LOCUS       RC205148                7614 bp    DNA     circular  SYN 
DEFINITION  BCAN (Myc-DDK-tagged)-Human brevican (BCAN), transcript variant 1
ACCESSION   NM_021948
VERSION     NM_021948.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3771..3862 
                     /label="Myc-DDK"  
     misc_feature    3892..4477 
                     /label="PolyA signal"  
     misc_feature    3949..3968 
                     /label="Restriction Enzyme"  
     misc_feature    4753..5425 
                     /label="ColE1"  
     misc_feature    5855..6649 
                     /label="Kan/Neo"  
     misc_feature    6684..6986 
                     /label="SV40 ori"  
     misc_feature    7072..7549 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3759 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="BCAN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGCTG TTCCTGCCCC TGCTGGCAGC CCTGGTCCTG GCCCAGGCTC 
     1081 CTGCAGCTTT AGCAGATGTT CTGGAAGGAG ACAGCTCAGA GGACCGCGCT TTTCGCGTGC 
     1141 GCATCGCGGG CGACGCGCCA CTGCAGGGCG TGCTCGGCGG CGCCCTCACC ATCCCTTGCC 
     1201 ACGTCCACTA CCTGCGGCCA CCGCCGAGCC GCCGGGCTGT GCTGGGCTCT CCGCGGGTCA 
     1261 AGTGGACTTT CCTGTCCCGG GGCCGGGAGG CAGAGGTGCT GGTGGCGCGG GGAGTGCGCG 
     1321 TCAAGGTGAA CGAGGCCTAC CGGTTCCGCG TGGCACTGCC TGCGTACCCA GCGTCGCTCA 
     1381 CCGACGTCTC CCTGGCGCTG AGCGAGCTGC GCCCCAACGA CTCAGGTATC TATCGCTGTG 
     1441 AGGTCCAGCA CGGCATCGAT GACAGCAGCG ACGCTGTGGA GGTCAAGGTC AAAGGGGTCG 
     1501 TCTTTCTCTA CCGAGAGGGC TCTGCCCGCT ATGCTTTCTC CTTTTCTGGG ACCCAGGAGG 
     1561 CCTGTGCCCG CATTGGAGCC CACATCGCCA CCCCGGAGCA GCTCTATGCC GCCTACCTTG 
     1621 GGGGCTATGA GCAATGTGAT GCTGGCTGGC TGTCGGATCA GACCGTGAGG TATCCCATCC 
     1681 AGACCCCACG AGAGGCCTGT TACGGAGACA TGGATGGCTT CCCCGGGGTC CGGAACTATG 
     1741 GTGTGGTGGA CCCGGATGAC CTCTATGATG TGTACTGTTA TGCTGAAGAC CTAAATGGAG 
     1801 AACTGTTCCT GGGTGACCCT CCAGAGAAGC TGACATTGGA GGAAGCACGG GCGTACTGCC 
     1861 AGGAGCGGGG TGCAGAGATT GCCACCACGG GCCAACTGTA TGCAGCCTGG GATGGTGGCC 
     1921 TGGACCACTG CAGCCCAGGG TGGCTAGCTG ATGGCAGTGT GCGCTACCCC ATCGTCACAC 
     1981 CCAGCCAGCG CTGTGGTGGG GGCTTGCCTG GTGTCAAGAC TCTCTTCCTC TTCCCCAACC 
     2041 AGACTGGCTT CCCCAATAAG CACAGCCGCT TCAACGTCTA CTGCTTCCGA GACTCGGCCC 
     2101 AGCCTTCTGC CATCCCTGAG GCCTCCAACC CAGCCTCCAA CCCAGCCTCT GATGGACTAG 
     2161 AGGCTATCGT CACAGTGACA GAGACCCTGG AGGAACTGCA GCTGCCTCAG GAAGCCACAG 
     2221 AGAGTGAATC CCGTGGGGCC ATCTACTCCA TCCCCATCAT GGAGGACGGA GGAGGTGGAA 
     2281 GCTCCACTCC AGAAGACCCA GCAGAGGCCC CTAGGACGCT CCTAGAATTT GAAACACAAT 
     2341 CCATGGTACC GCCCACGGGG TTCTCAGAAG AGGAAGGTAA GGCATTGGAG GAAGAAGAGA 
     2401 AATATGAAGA TGAAGAAGAG AAAGAGGAGG AAGAAGAAGA GGAGGAGGTG GAGGATGAGG 
     2461 CTCTGTGGGC ATGGCCCAGC GAGCTCAGCA GCCCGGGCCC TGAGGCCTCT CTCCCCACTG 
     2521 AGCCAGCAGC CCAGGAGGAG TCACTCTCCC AGGCGCCAGC AAGGGCAGTC CTGCAGCCTG 
     2581 GTGCATCACC ACTTCCTGAT GGAGAGTCAG AAGCTTCCAG GCCTCCAAGG GTCCATGGAC 
     2641 CACCTACTGA GACTCTGCCC ACTCCCAGGG AGAGGAACCT AGCATCCCCA TCACCTTCCA 
     2701 CTCTGGTTGA GGCAAGAGAG GTGGGGGAGG CAACTGGTGG TCCTGAGCTA TCTGGGGTCC 
     2761 CTCGAGGAGA GAGCGAGGAG ACAGGAAGCT CCGAGGGTGC CCCTTCCCTG CTTCCAGCCA 
     2821 CACGGGCCCC TGAGGGTACC AGGGAGCTGG AGGCCCCCTC TGAAGATAAT TCTGGAAGAA 
     2881 CTGCCCCAGC AGGGACCTCA GTGCAGGCCC AGCCAGTGCT GCCCACTGAC AGCGCCAGCC 
     2941 GAGGTGGAGT GGCCGTGGTC CCCGCATCAG GTGACTGTGT CCCCAGCCCC TGCCACAATG 
     3001 GTGGGACATG CTTGGAGGAG GAGGAAGGGG TCCGCTGCCT ATGTCTGCCT GGCTATGGGG 
     3061 GGGACCTGTG CGATGTTGGC CTCCGCTTCT GCAACCCCGG CTGGGACGCC TTCCAGGGCG 
     3121 CCTGCTACAA GCACTTTTCC ACACGAAGGA GCTGGGAGGA GGCAGAGACC CAGTGCCGGA 
     3181 TGTACGGCGC GCATCTGGCC AGCATCAGCA CACCCGAGGA ACAGGACTTC ATCAACAACC 
     3241 GGTACCGGGA GTACCAGTGG ATCGGACTCA ACGACAGGAC CATCGAAGGC GACTTCTTGT 
     3301 GGTCGGATGG CGTCCCCCTG CTCTATGAGA ACTGGAACCC TGGGCAGCCT GACAGCTACT 
     3361 TCCTGTCTGG AGAGAACTGC GTGGTCATGG TGTGGCATGA TCAGGGACAA TGGAGTGACG 
     3421 TGCCCTGCAA CTACCACCTG TCCTACACCT GCAAGATGGG GCTGGTGTCC TGTGGGCCGC 
     3481 CACCGGAGCT GCCCCTGGCT CAAGTGTTCG GCCGCCCACG GCTGCGCTAT GAGGTGGACA 
     3541 CTGTGCTTCG CTACCGGTGC CGGGAAGGAC TGGCCCAGCG CAATCTGCCG CTGATCCGAT 
     3601 GCCAAGAGAA CGGTCGTTGG GAGGCCCCCC AGATCTCCTG TGTGCCCAGA AGACCTGCCC 
     3661 GAGCTCTGCA CCCAGAGGAG GACCCAGAAG GACGTCAGGG GAGGCTACTG GGACGCTGGA 
     3721 AGGCGCTGTT GATCCCCCCT TCCAGCCCCA TGCCAGGTCC CACGCGTACG CGGCCGCTCG 
     3781 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3841 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3901 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3961 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4021 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4081 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4141 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4201 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4261 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4321 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4381 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4441 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4501 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4561 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4621 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5281 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5341 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5401 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5461 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5521 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5581 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5641 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5701 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5761 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5821 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5881 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5941 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6001 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6061 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6121 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6181 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6241 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6301 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6361 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6421 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6481 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6541 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6601 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6661 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6721 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6781 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6841 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6901 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6961 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7021 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7081 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7141 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7201 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7261 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7321 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7381 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7441 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7501 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7561 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//