LOCUS       RC205133L1                9258 bp    DNA     circular  SYN 
DEFINITION  FGD1 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 1 (FGD1)
ACCESSION   NM_004463
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7549..7729 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8457..9116 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7888..8456 
                     /label="ColE1"  
     misc_feature    6424..6444 
                     /label="LR50 primer"  
     misc_feature    6221..6292 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6433..7024 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6203..6209 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6203 
                     /label="FGD1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCATGGCCAC CGAGCCCCGG GGGGCGCCGG 
     3361 GCCTTCGGAG CCCGAACACC CGGCCACGAA CCCGCCGGGC GCCGCTCCGC CGGCCTGTGC 
     3421 CGACTCGGAC CCTGGAGCCT CGGAACCCGG ACTGCTGGCG CGCAGGGGCT CAGGTTCGGC 
     3481 TCTTGGCGGC CCACTGGATC CCCAGTTTGT CGGACCCTCG GACACCAGCC TGGGCGCTGC 
     3541 TCCAGGCCAC CGGGTCTTGC CCTGCGGTCC CAGTCCACAG CACCACCGGG CCCTGCGCTT 
     3601 CTCTTACCAC CTGGAGGGCT CGCAGCCTCG GCCTGGGCTG CACCAGGGAA ACCGGATCCT 
     3661 GGTTAAAAGT TTGTCCCTTG ACCCTGGCCA AAGCCTAGAG CCTCATCCAG AAGGTCCCCA 
     3721 GCGGCTTCGC TCAGACCCAG GTCCCCCGAC TGAAACCCCT AGCCAGCGTC CTTCACCACT 
     3781 GAAGCGGGCA CCGGGCCCGA AGCCACAGGT GCCCCCAAAG CCCAGCTACC TGCAGATGCC 
     3841 CCGGATGCCC CCCCCACTGG AGCCCATCCC CCCTCCACCA TCACGCCCAC TGCCTGCCGA 
     3901 CCCCCGAGTG GCCAAGGGCC TGGCTCCCAG GGCAGAGGCC AGCCCCAGTT CTGCAGCAGT 
     3961 ATCCTCACTG ATTGAGAAGT TTGAAAGAGA GCCTGTGATT GTCGCCTCGG ATAGACCAGT 
     4021 CCCTGGCCCC AGCCCAGGTC CCCCAGAGCC AGTCATGTTG CCACAGCCAA CCTCGCAGCC 
     4081 ACCAGTGCCC CAGCTCCCCG AGGGTGAGGC CTCCCGCTGC CTGTTTCTGC TGGCTCCTGG 
     4141 GCCCCGGGAC GGTGAGAAGG TGCCCAACCG GGACAGCGGC ATTGATAGCA TCAGCTCGCC 
     4201 ATCCAACAGC GAGGAGACCT GCTTCGTCAG TGATGACGGG CCCCCCAGCC ACAGCCTCTG 
     4261 CCCTGGGCCC CCTGCCCTGG CTAGTGTGCC TGTTGCCTTG GCCGACCCCC ACCGGCCTGG 
     4321 CTCCCAAGAG GTTGACAGTG ACCTGGAGGA GGAGGACGAC GAGGAGGAGG AGGAAGAGAA 
     4381 GGACAGAGAA ATCCCAGTGC CCCTGATGGA GAGACAGGAG TCTGTGGAGT TGACTGTGCA 
     4441 GCAAAAGGTG TTTCACATTG CCAATGAGCT CCTGCAAACT GAGAAGGCCT ACGTTTCCAG 
     4501 GCTCCATCTC CTGGATCAGG TGTTCTGTGC CCGGCTGCTG GAAGAAGCTC GGAACCGCAG 
     4561 TTCCTTCCCG GCCGACGTTG TCCACGGCAT CTTCTCTAAC ATCTGCTCCA TCTATTGCTT 
     4621 CCACCAGCAG TTCCTGCTGC CTGAGCTAGA GAAGCGCATG GAGGAATGGG ACCGCTATCC 
     4681 ACGCATTGGA GACATCCTGC AGAAACTGGC CCCCTTCCTC AAGATGTATG GTGAGTATGT 
     4741 GAAGAACTTT GACCGGGCCG TGGAGCTGGT CAACACCTGG ACAGAGCGCT CCACCCAGTT 
     4801 TAAAGTCATC ATCCATGAGG TGCAGAAGGA GGAAGCCTGT GGCAACCTGA CATTGCAGCA 
     4861 CCACATGCTG GAGCCTGTGC AGCGCATCCC CCGCTATGAG CTTCTTCTCA AGGACTATCT 
     4921 GTTAAAGCTG CCCCATGGCT CCCCGGACAG CAAGGATGCC CAAAAGTCTC TGGAGCTGAT 
     4981 CGCCACAGCA GCAGAGCACT CGAATGCTGC CATCCGCAAA ATGGAGCGAA TGCATAAGCT 
     5041 GCTGAAGGTA TATGAGCTGT TAGGGGGCGA GGAGGACATT GTCAGCCCCA CCAAAGAGCT 
     5101 CATAAAAGAA GGCCACATCC TTAAGCTGTC AGCAAAGAAT GGGACCACTC AAGACCGATA 
     5161 CCTCATACTA TTCAACGACC GCCTCCTTTA CTGCGTGCCC AGGCTGCGGC TCCTTGGCCA 
     5221 GAAGTTTAGC GTGCGGGCAC GCATTGATGT AGATGGCATG GAGCTAAAGG AGAGCTCCAA 
     5281 CCTCAATCTG CCTCGAACCT TCCTGGTGTC AGGAAAGCAG CGCTCCCTCG AGCTCCAGGC 
     5341 CAGGACTGAG GAGGAGAAGA AAGACTGGGT CCAGGCCATC AACTCCACCC TCCTGAAGCA 
     5401 TGAACAGACG CTGGAGACTT TCAAACTGTT GAACTCAACA AACAGGGAAG ATGAAGACAC 
     5461 CCCACCCAAC TCTCCAAACG TGGATCTTGG GAAGCGGGCA CCTACGCCCA TCCGGGAAAA 
     5521 GGAAGTCACC ATGTGCATGC GCTGCCAGGA GCCCTTCAAT TCTATCACCA AACGCAGGCA 
     5581 CCACTGCAAG GCCTGCGGGC ATGTGGTTTG TGGGAAGTGC TCCGAGTTCC GGGCCCGCCT 
     5641 CGTCTATGAC AACAACCGCT CCAACCGTGT GTGCACTGAT TGCTATGTGG CCTTGCACGG 
     5701 GGTGCCTGGG AGCAGTCCAG CCTGCAGCCA GCATACACCC CAGCGCCGGA GGTCCATCCT 
     5761 GGAGAAACAG GCCTCAGTGG CTGCAGAGAA CAGCGTCATC TGCAGCTTCC TGCACTACAT 
     5821 GGAGAAGGGT GGCAAAGGAT GGCACAAGGC ATGGTTCGTG GTCCCTGAAA ATGAACCCTT 
     5881 GGTGCTGTAT ATCTACGGAG CCCCTCAGGA TGTGAAAGCC CAGCGCAGCC TGCCCCTCAT 
     5941 TGGCTTCGAG GTGGGACCGC CCGAGGCAGG GGAGCGGCCT GACAGAAGGC ATGTCTTCAA 
     6001 GATCACCCAG AGCCACCTCA GCTGGTACTT CAGCCCTGAG ACAGAGGAAC TACAGCGACG 
     6061 CTGGATGGCT GTGCTTGGCC GGGCGGGCCG AGGGGACACG TTCTGCCCGG GGCCCACACT 
     6121 GTCTGAGGAC AGGGAGATGG AGGAGGCACC GGTGGCTGCT TTAGGAGCCA CTGCTGAACC 
     6181 CCCCGAATCC CCCCAGACCC GAGACAAGAC CACGCGTACG CGGCCGCTCG AGCAGAAACT 
     6241 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     6301 GGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 
     6361 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 
     6421 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 
     6481 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 
     6541 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 
     6601 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 
     6661 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 
     6721 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 
     6781 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 
     6841 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 
     6901 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 
     6961 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 
     7021 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 
     7081 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 
     7141 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 
     7201 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 
     7261 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 
     7321 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 
     7381 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 
     7441 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 
     7501 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 
     7561 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 
     7621 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 
     7681 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 
     7741 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     7801 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     7861 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     7921 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     7981 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     8041 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     8101 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     8161 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     8221 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     8281 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     8341 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     8401 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     8461 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
     8521 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 
     8581 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 
     8641 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 
     8701 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 
     8761 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
     8821 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 
     8881 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 
     8941 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 
     9001 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 
     9061 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 
     9121 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     9181 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTCCCGCG CACATTTCCC 
     9241 CGAAAAGTGC CACCTGAC
//