LOCUS       RC205133                7764 bp    DNA     circular  SYN 
DEFINITION  FGD1 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 1 (FGD1)
ACCESSION   NM_004463
VERSION     NM_004463.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3921..4012 
                     /label="Myc-DDK"  
     misc_feature    4042..4627 
                     /label="PolyA signal"  
     misc_feature    4099..4118 
                     /label="Restriction Enzyme"  
     misc_feature    4903..5575 
                     /label="ColE1"  
     misc_feature    6005..6799 
                     /label="Kan/Neo"  
     misc_feature    6834..7136 
                     /label="SV40 ori"  
     misc_feature    7222..7699 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3903..3909 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3903 
                     /label="FGD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATGGCCAC CGAGCCCCGG GGGGCGCCGG GCCTTCGGAG CCCGAACACC 
     1081 CGGCCACGAA CCCGCCGGGC GCCGCTCCGC CGGCCTGTGC CGACTCGGAC CCTGGAGCCT 
     1141 CGGAACCCGG ACTGCTGGCG CGCAGGGGCT CAGGTTCGGC TCTTGGCGGC CCACTGGATC 
     1201 CCCAGTTTGT CGGACCCTCG GACACCAGCC TGGGCGCTGC TCCAGGCCAC CGGGTCTTGC 
     1261 CCTGCGGTCC CAGTCCACAG CACCACCGGG CCCTGCGCTT CTCTTACCAC CTGGAGGGCT 
     1321 CGCAGCCTCG GCCTGGGCTG CACCAGGGAA ACCGGATCCT GGTTAAAAGT TTGTCCCTTG 
     1381 ACCCTGGCCA AAGCCTAGAG CCTCATCCAG AAGGTCCCCA GCGGCTTCGC TCAGACCCAG 
     1441 GTCCCCCGAC TGAAACCCCT AGCCAGCGTC CTTCACCACT GAAGCGGGCA CCGGGCCCGA 
     1501 AGCCACAGGT GCCCCCAAAG CCCAGCTACC TGCAGATGCC CCGGATGCCC CCCCCACTGG 
     1561 AGCCCATCCC CCCTCCACCA TCACGCCCAC TGCCTGCCGA CCCCCGAGTG GCCAAGGGCC 
     1621 TGGCTCCCAG GGCAGAGGCC AGCCCCAGTT CTGCAGCAGT ATCCTCACTG ATTGAGAAGT 
     1681 TTGAAAGAGA GCCTGTGATT GTCGCCTCGG ATAGACCAGT CCCTGGCCCC AGCCCAGGTC 
     1741 CCCCAGAGCC AGTCATGTTG CCACAGCCAA CCTCGCAGCC ACCAGTGCCC CAGCTCCCCG 
     1801 AGGGTGAGGC CTCCCGCTGC CTGTTTCTGC TGGCTCCTGG GCCCCGGGAC GGTGAGAAGG 
     1861 TGCCCAACCG GGACAGCGGC ATTGATAGCA TCAGCTCGCC ATCCAACAGC GAGGAGACCT 
     1921 GCTTCGTCAG TGATGACGGG CCCCCCAGCC ACAGCCTCTG CCCTGGGCCC CCTGCCCTGG 
     1981 CTAGTGTGCC TGTTGCCTTG GCCGACCCCC ACCGGCCTGG CTCCCAAGAG GTTGACAGTG 
     2041 ACCTGGAGGA GGAGGACGAC GAGGAGGAGG AGGAAGAGAA GGACAGAGAA ATCCCAGTGC 
     2101 CCCTGATGGA GAGACAGGAG TCTGTGGAGT TGACTGTGCA GCAAAAGGTG TTTCACATTG 
     2161 CCAATGAGCT CCTGCAAACT GAGAAGGCCT ACGTTTCCAG GCTCCATCTC CTGGATCAGG 
     2221 TGTTCTGTGC CCGGCTGCTG GAAGAAGCTC GGAACCGCAG TTCCTTCCCG GCCGACGTTG 
     2281 TCCACGGCAT CTTCTCTAAC ATCTGCTCCA TCTATTGCTT CCACCAGCAG TTCCTGCTGC 
     2341 CTGAGCTAGA GAAGCGCATG GAGGAATGGG ACCGCTATCC ACGCATTGGA GACATCCTGC 
     2401 AGAAACTGGC CCCCTTCCTC AAGATGTATG GTGAGTATGT GAAGAACTTT GACCGGGCCG 
     2461 TGGAGCTGGT CAACACCTGG ACAGAGCGCT CCACCCAGTT TAAAGTCATC ATCCATGAGG 
     2521 TGCAGAAGGA GGAAGCCTGT GGCAACCTGA CATTGCAGCA CCACATGCTG GAGCCTGTGC 
     2581 AGCGCATCCC CCGCTATGAG CTTCTTCTCA AGGACTATCT GTTAAAGCTG CCCCATGGCT 
     2641 CCCCGGACAG CAAGGATGCC CAAAAGTCTC TGGAGCTGAT CGCCACAGCA GCAGAGCACT 
     2701 CGAATGCTGC CATCCGCAAA ATGGAGCGAA TGCATAAGCT GCTGAAGGTA TATGAGCTGT 
     2761 TAGGGGGCGA GGAGGACATT GTCAGCCCCA CCAAAGAGCT CATAAAAGAA GGCCACATCC 
     2821 TTAAGCTGTC AGCAAAGAAT GGGACCACTC AAGACCGATA CCTCATACTA TTCAACGACC 
     2881 GCCTCCTTTA CTGCGTGCCC AGGCTGCGGC TCCTTGGCCA GAAGTTTAGC GTGCGGGCAC 
     2941 GCATTGATGT AGATGGCATG GAGCTAAAGG AGAGCTCCAA CCTCAATCTG CCTCGAACCT 
     3001 TCCTGGTGTC AGGAAAGCAG CGCTCCCTCG AGCTCCAGGC CAGGACTGAG GAGGAGAAGA 
     3061 AAGACTGGGT CCAGGCCATC AACTCCACCC TCCTGAAGCA TGAACAGACG CTGGAGACTT 
     3121 TCAAACTGTT GAACTCAACA AACAGGGAAG ATGAAGACAC CCCACCCAAC TCTCCAAACG 
     3181 TGGATCTTGG GAAGCGGGCA CCTACGCCCA TCCGGGAAAA GGAAGTCACC ATGTGCATGC 
     3241 GCTGCCAGGA GCCCTTCAAT TCTATCACCA AACGCAGGCA CCACTGCAAG GCCTGCGGGC 
     3301 ATGTGGTTTG TGGGAAGTGC TCCGAGTTCC GGGCCCGCCT CGTCTATGAC AACAACCGCT 
     3361 CCAACCGTGT GTGCACTGAT TGCTATGTGG CCTTGCACGG GGTGCCTGGG AGCAGTCCAG 
     3421 CCTGCAGCCA GCATACACCC CAGCGCCGGA GGTCCATCCT GGAGAAACAG GCCTCAGTGG 
     3481 CTGCAGAGAA CAGCGTCATC TGCAGCTTCC TGCACTACAT GGAGAAGGGT GGCAAAGGAT 
     3541 GGCACAAGGC ATGGTTCGTG GTCCCTGAAA ATGAACCCTT GGTGCTGTAT ATCTACGGAG 
     3601 CCCCTCAGGA TGTGAAAGCC CAGCGCAGCC TGCCCCTCAT TGGCTTCGAG GTGGGACCGC 
     3661 CCGAGGCAGG GGAGCGGCCT GACAGAAGGC ATGTCTTCAA GATCACCCAG AGCCACCTCA 
     3721 GCTGGTACTT CAGCCCTGAG ACAGAGGAAC TACAGCGACG CTGGATGGCT GTGCTTGGCC 
     3781 GGGCGGGCCG AGGGGACACG TTCTGCCCGG GGCCCACACT GTCTGAGGAC AGGGAGATGG 
     3841 AGGAGGCACC GGTGGCTGCT TTAGGAGCCA CTGCTGAACC CCCCGAATCC CCCCAGACCC 
     3901 GAGACAAGAC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3961 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4021 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4081 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4141 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4201 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4261 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4321 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4381 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4441 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4501 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4561 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4621 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4681 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4741 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4801 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5401 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5461 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5521 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5581 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5641 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5701 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5761 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5821 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5881 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5941 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6001 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6061 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6121 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6181 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6241 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6301 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6361 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6421 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6481 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6541 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6601 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6661 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6721 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6781 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6841 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6901 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6961 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7021 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7081 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7201 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7261 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7321 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7381 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7441 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7501 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7561 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7621 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7681 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7741 TAACAAAAAT TTAACGCGAA TTTT
//