LOCUS       RC205058L4                9482 bp    DNA     circular  SYN 
DEFINITION  ARSF (Myc-DDK-tagged)-Human arylsulfatase F (ARSF), transcript variant 1
ACCESSION   NM_004042
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7773..7953 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8681..9327 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    6648..6668 
                     /label="LR50"  
     misc_feature    5111..5839 
                     /label="mGFP"  
     misc_feature    5846..5911 
                     /label="P2A"  
     misc_feature    8001..8620 
                     /label="pBR322 Ori"  
     misc_feature    5912..6511 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5090..5096 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5090 
                     /label="ARSF"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGGCCCAGG AGACCCTTGG TCTTCATGTC 
     3361 TTTGGTGTGT GCACTCTTGA ACACATGCCA GGCACACAGG GTGCATGACG ACAAGCCTAA 
     3421 TATTGTCCTA ATCATGGTTG ATGACCTGGG TATTGGAGAT CTGGGCTGCT ACGGCAATGA 
     3481 CACCATGAGG ACGCCTCACA TCGACCGCCT TGCCAGGGAA GGCGTGCGAC TGACTCAGCA 
     3541 CATCTCTGCC GCCTCCCTCT GCAGCCCAAG CCGGTCCGCG TTCTTGACGG GAAGATACCC 
     3601 CATCCGATCA GGTATGGTTT CTAGTGGTAA TAGACGTGTC ATCCAAAATC TTGCAGTCCC 
     3661 CGCAGGCCTC CCTCTTAATG AGACAACACT TGCAGCCTTG CTAAAGAAGC AAGGATACAG 
     3721 CACGGGGCTT ATAGGCAAAT GGCACCAAGG CTTGAACTGC GACTCCCGAA GTGACCAGTG 
     3781 CCACCATCCA TATAATTATG GGTTTGACTA CTACTATGGC ATGCCGTTCA CTCTCGTTGA 
     3841 CAGCTGCTGG CCGGACCCCT CTCGTAACAC GGAATTAGCC TTTGAGAGTC AGCTCTGGCT 
     3901 CTGTGTGCAG CTAGTTGCCA TTGCCATCCT CACCCTAACC TTTGGGAAGC TGAGCGGCTG 
     3961 GGTCTCTGTT CCCTGGCTCC TGATCTTCTC CATGATTCTG TTTATTTTCC TCTTGGGCTA 
     4021 TGCTTGGTTC TCCAGCCACA CGTCCCCTTT ATACTGGGAC TGCCTCCTCA TGCGGGGGCA 
     4081 CGAGATCACG GAGCAGCCCA TGAAGGCTGA ACGAGCTGGA TCCATTATGG TGAAGGAAGC 
     4141 GATTTCCTTT TTAGAAAGGC ACAGTAAGGA AACTTTCCTT CTCTTTTTCT CCTTTCTTCA 
     4201 CGTGCACACA CCTCTCCCCA CCACGGACGA TTTCACTGGC ACCAGCAAGC ATGGCTTGTA 
     4261 TGGGGATAAT GTGGAAGAGA TGGACTCCAT GGTGGGCAAG ATTCTTGATG CTATCGATGA 
     4321 TTTTGGCCTA AGGAACAACA CCCTTGTCTA CTTTACATCA GATCACGGAG GGCATTTGGA 
     4381 AGCTAGGCGA GGGCATGCCC AACTTGGTGG ATGGAATGGA ATATACAAAG GTGGAAAAGG 
     4441 CATGGGGGGC TGGGAAGGTG GAATCCGCGT CCCAGGAATT GTCCGATGGC CTGGAAAGGT 
     4501 ACCAGCTGGA CGGTTGATTA AGGAACCTAC AAGTTTAATG GATATTTTAC CAACTGTCGC 
     4561 ATCAGTGTCA GGAGGAAGTC TCCCTCAGGA CAGGGTCATT GACGGCCGAG ACCTCATGCC 
     4621 CTTGCTGCAG GGCAACGTCA GGCACTCGGA GCATGAATTT CTTTTCCACT ACTGTGGCTC 
     4681 CTACCTGCAC GCCGTGCGGT GGATCCCCAA GGACGACAGT GGGTCAGTTT GGAAGGCTCA 
     4741 CTATGTGACC CCGGTATTCC AGCCACCAGC TTCTGGTGGC TGCTATGTCA CCTCATTATG 
     4801 CAGATGTTTC GGAGAACAGG TTACCTACCA CAACCCCCCT CTGCTCTTCG ATCTCTCCAG 
     4861 GGACCCCTCA GAGTCCACAC CCCTGACACC TGCCACAGAG CCCCTCTATG ATTTTGTGAT 
     4921 TAAAAAGGTG GCCAACGCCC TGAAGGAACA CCAGGAAACC ATCGTGCCTG TGACCTACCA 
     4981 ACTCTCAGAA CTGAATCAGG GCAGGACGTG GCTGAAGCCT TGCTGTGGGG TGTTCCCATT 
     5041 TTGTCTGTGT GACAAGGAAG AGGAAGTCTC TCAGCCTCGG GGTCCTAACG AGAAGAGAAC 
     5101 GCGTACGCGG CCGCTCGAGA TGAGCGGGGG CGAGGAGCTG TTCGCCGGCA TCGTGCCCGT 
     5161 GCTGATCGAG CTGGACGGCG ACGTGCACGG CCACAAGTTC AGCGTGCGCG GCGAGGGCGA 
     5221 GGGCGACGCC GACTACGGCA AGCTGGAGAT CAAGTTCATC TGCACCACCG GCAAGCTGCC 
     5281 CGTGCCCTGG CCCACCCTGG TGACCACCCT CTGCTACGGC ATCCAGTGCT TCGCCCGCTA 
     5341 CCCCGAGCAC ATGAAGATGA ACGACTTCTT CAAGAGCGCC ATGCCCGAGG GCTACATCCA 
     5401 GGAGCGCACC ATCCAGTTCC AGGACGACGG CAAGTACAAG ACCCGCGGCG AGGTGAAGTT 
     5461 CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC AAGGACTTCA AGGAGGACGG 
     5521 CAACATCCTG GGCCACAAGC TGGAGTACAG CTTCAACAGC CACAACGTGT ACATCCGCCC 
     5581 CGACAAGGCC AACAACGGCC TGGAGGCTAA CTTCAAGACC CGCCACAACA TCGAGGGCGG 
     5641 CGGCGTGCAG CTGGCCGACC ACTACCAGAC CAACGTGCCC CTGGGCGACG GCCCCGTGCT 
     5701 GATCCCCATC AACCACTACC TGAGCACTCA GACCAAGATC AGCAAGGACC GCAACGAGGC 
     5761 CCGCGACCAC ATGGTGCTCC TGGAGTCCTT CAGCGCCTGC TGCCACACCC ACGGCATGGA 
     5821 CGAGCTGTAC AGGTCCGGAC TCAGAGTTTG GGTAGGAAGC GGAGCTACTA ACTTCAGCCT 
     5881 GCTGAAGCAG GCTGGAGACG TGGAGGAGAA CCCTGGACCT ATGACCGAGT ACAAGCCCAC 
     5941 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT 
     6001 CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAT CGCCACATCG AGCGGGTCAC 
     6061 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC 
     6121 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT 
     6181 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA 
     6241 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC 
     6301 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG 
     6361 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA 
     6421 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG 
     6481 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA TGTACACCCA AACGGCCGGC 
     6541 CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT 
     6601 ACGAAGTTAT ACATGTTTAA GGGTTCCGGT TCCACTAGGT ACAATTCGAT ATCAAGCTTA 
     6661 TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 
     6721 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 
     6781 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 
     6841 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 
     6901 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 
     6961 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 
     7021 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC 
     7081 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 
     7141 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 
     7201 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCATCGAT 
     7261 ACCGTCGACC TCGATCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG CAATACAGCA 
     7321 GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT GGGTTTTCCA 
     7381 GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC 
     7441 TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATATC 
     7501 CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA CTACACACCA 
     7561 GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT ACCAGTTGAG 
     7621 CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA CCCTGTGAGC 
     7681 CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA CAGCCGCCTA 
     7741 GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT CTGGTTAGAC 
     7801 CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA 
     7861 AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG 
     7921 AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATGTG AGCAAAAGGC 
     7981 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 
     8041 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 
     8101 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC 
     8161 CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT 
     8221 AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 
     8281 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 
     8341 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 
     8401 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT 
     8461 AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT 
     8521 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 
     8581 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG 
     8641 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAT TACGCCCCGC 
     8701 CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT 
     8761 CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT 
     8821 AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT 
     8881 CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC 
     8941 CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA 
     9001 GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT 
     9061 CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA 
     9121 TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG 
     9181 GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA 
     9241 CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT 
     9301 GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATA CTCTTCCTTT 
     9361 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     9421 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     9481 AC
//