LOCUS       RC204924L1                9051 bp    DNA     circular  SYN 
DEFINITION  ACTN1 (Myc-DDK-tagged)-Human actinin, alpha 1 (ACTN1), transcript variant 2
ACCESSION   NM_001102
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7342..7522 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8250..8909 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7681..8249 
                     /label="ColE1"  
     misc_feature    6217..6237 
                     /label="LR50 primer"  
     misc_feature    6014..6085 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6226..6817 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5996..6002 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5996 
                     /label="ACTN1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGACCATTAT GATTCTCAGC AAACCAACGA 
     3361 TTACATGCAG CCAGAAGAGG ACTGGGACCG GGACCTGCTC CTGGACCCGG CCTGGGAGAA 
     3421 GCAGCAGAGA AAGACATTCA CGGCATGGTG TAACTCCCAC CTCCGGAAGG CGGGGACACA 
     3481 GATCGAGAAC ATCGAAGAGG ACTTCCGGGA TGGCCTGAAG CTCATGCTGC TGCTGGAGGT 
     3541 CATCTCAGGT GAACGCTTGG CCAAGCCAGA GCGAGGCAAG ATGAGAGTGC ACAAGATCTC 
     3601 CAACGTCAAC AAGGCCCTGG ATTTCATAGC CAGCAAAGGC GTCAAACTGG TGTCCATCGG 
     3661 AGCCGAAGAA ATCGTGGATG GGAATGTGAA GATGACCCTG GGCATGATCT GGACCATCAT 
     3721 CCTGCGCTTT GCCATCCAGG ACATCTCCGT GGAAGAGACT TCAGCCAAGG AAGGGCTGCT 
     3781 CCTGTGGTGT CAGAGAAAGA CAGCCCCTTA CAAAAATGTC AACATCCAGA ACTTCCACAT 
     3841 AAGCTGGAAG GATGGCCTCG GCTTCTGTGC TTTGATCCAC CGACACCGGC CCGAGCTGAT 
     3901 TGACTACGGG AAGCTGCGGA AGGATGATCC ACTCACAAAT CTGAATACGG CTTTTGACGT 
     3961 GGCAGAGAAG TACCTGGACA TCCCCAAGAT GCTGGATGCC GAAGACATCG TTGGAACTGC 
     4021 CCGACCGGAT GAGAAAGCCA TCATGACTTA CGTGTCTAGC TTCTACCACG CCTTCTCTGG 
     4081 AGCCCAGAAG GCGGAGACAG CAGCCAATCG CATCTGCAAG GTGTTGGCCG TCAACCAGGA 
     4141 GAACGAGCAG CTTATGGAAG ACTACGAGAA GCTGGCCAGT GATCTGTTGG AGTGGATCCG 
     4201 CCGCACAATC CCGTGGCTGG AGAACCGGGT GCCCGAGAAC ACCATGCATG CCATGCAACA 
     4261 GAAGCTGGAG GACTTCCGGG ACTACCGGCG CCTGCACAAG CCGCCCAAGG TGCAGGAGAA 
     4321 GTGCCAGCTG GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGGCTCA GCAACCGGCC 
     4381 TGCCTTCATG CCCTCTGAGG GCAGGATGGT CTCGGACATC AACAATGCCT GGGGCTGCCT 
     4441 GGAGCAGGTG GAGAAGGGCT ATGAGGAGTG GTTGCTGAAT GAGATCCGGA GGCTGGAGCG 
     4501 ACTGGACCAC CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACTGA 
     4561 CGGCAAAGAG GCCATGCTGC GACAGAAGGA CTATGAGACC GCCACCCTCT CGGAGATCAA 
     4621 GGCCCTGCTC AAGAAGCATG AGGCCTTCGA GAGTGACCTG GCTGCCCACC AGGACCGTGT 
     4681 GGAGCAGATT GCCGCCATCG CACAGGAGCT CAATGAGCTG GACTATTATG ACTCACCCAG 
     4741 TGTCAACGCC CGTTGCCAAA AGATCTGTGA CCAGTGGGAC AATCTGGGGG CCCTAACTCA 
     4801 GAAGCGAAGG GAAGCTCTGG AGCGGACCGA GAAACTGCTG GAGACCATTG ACCAGCTGTA 
     4861 CTTGGAGTAT GCCAAGCGGG CTGCACCCTT CAACAACTGG ATGGAGGGGG CCATGGAGGA 
     4921 CCTGCAGGAC ACCTTCATTG TGCACACCAT TGAGGAGATC CAGGGACTGA CCACAGCCCA 
     4981 TGAGCAGTTC AAGGCCACCC TCCCTGATGC CGACAAGGAG CGCCTGGCCA TCCTGGGCAT 
     5041 CCACAATGAG GTGTCCAAGA TTGTCCAGAC CTACCACGTC AATATGGCGG GCACCAACCC 
     5101 CTACACAACC ATCACGCCTC AGGAGATCAA TGGCAAATGG GACCACGTGC GGCAGCTGGT 
     5161 GCCTCGGAGG GACCAAGCTC TGACGGAGGA GCATGCCCGA CAGCAGCACA ATGAGAGGCT 
     5221 ACGCAAGCAG TTTGGAGCCC AGGCCAATGT CATCGGGCCC TGGATCCAGA CCAAGATGGA 
     5281 GGAGATCGGG AGGATCTCCA TTGAGATGCA TGGGACCCTG GAGGACCAGC TCAGCCACCT 
     5341 GCGGCAGTAT GAGAAGAGCA TCGTCAACTA CAAGCCAAAG ATTGATCAGC TGGAGGGCGA 
     5401 CCACCAGCTC ATCCAGGAGG CGCTCATCTT CGACAACAAG CACACCAACT ACACCATGGA 
     5461 GCACATCCGT GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCAGGACCA TCAATGAGGT 
     5521 AGAGAACCAG ATCCTGACCC GGGATGCCAA GGGCATCAGC CAGGAGCAGA TGAATGAGTT 
     5581 CCGGGCCTCC TTCAACCACT TTGACCGGGA TCACTCCGGC ACACTGGGTC CCGAGGAGTT 
     5641 CAAAGCCTGC CTCATCAGCT TGGGTTATGA TATTGGCAAC GACCCCCAGG GAGAAGCAGA 
     5701 ATTTGCCCGC ATCATGAGCA TTGTGGACCC CAACCGCCTG GGGGTAGTGA CATTCCAGGC 
     5761 CTTCATTGAC TTCATGTCCC GCGAGACAGC CGACACAGAT ACAGCAGACC AAGTCATGGC 
     5821 TTCCTTCAAG ATCCTGGCTG GGGACAAGAA CTACATTACC ATGGACGAGC TGCGCCGCGA 
     5881 GCTGCCACCC GACCAGGCTG AGTACTGCAT CGCGCGGATG GCCCCCTACA CCGGCCCCGA 
     5941 CTCCGTGCCA GGTGCTCTGG ACTACATGTC CTTCTCCACG GCGCTGTACG GCGAGAGTGA 
     6001 CCTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA 
     6061 TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA 
     6121 CAAGTAGGAT TCGTCGAGGG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 
     6181 CATGTTTAAG GGTTCCGGTT CCACTAGGTA CAATTCGATA TCAAGCTTAT CGATAATCAA 
     6241 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 
     6301 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 
     6361 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 
     6421 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 
     6481 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 
     6541 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 
     6601 ACTGACAATT CCGTGGTGTT GTCGGGGAAA TCATCGTCCT TTCCTTGGCT GCTCGCCTGT 
     6661 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 
     6721 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 
     6781 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCATCGATA CCGTCGACCT 
     6841 CGATCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC 
     6901 TGATTGTGCC TGGCTAGAAG CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA 
     6961 GGTACCTTTA AGACCAATGA CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA 
     7021 AAAGGGGGGA CTGGAAGGGC TAATTCACTC CCAACGAAGA CAAGATATCC TTGATCTGTG 
     7081 GATCTACCAC ACACAAGGCT ACTTCCCTGA TTGGCAGAAC TACACACCAG GGCCAGGGAT 
     7141 CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT 
     7201 AGAAGAAGCC AATGAAGGAG AGAACACCCG CTTGTTACAC CCTGTGAGCC TGCATGGGAT 
     7261 GGATGACCCG GAGAGAGAAG TATTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA 
     7321 CATGGCCCGA GAGCTGCATC CGGACTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC 
     7381 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG 
     7441 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG 
     7501 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGCATGTGA GCAAAAGGCC AGCAAAAGGC 
     7561 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     7621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     7681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     7741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     7801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     7861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     7921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     7981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     8041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     8101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     8161 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     8221 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGATT ACGCCCCGCC CTGCCACTCA 
     8281 TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA 
     8341 TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC 
     8401 ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG 
     8461 AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA 
     8521 TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG 
     8581 AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG 
     8641 GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG 
     8701 AACTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG 
     8761 TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA 
     8821 TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT 
     8881 ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATAC TCTTCCTTTT TCAATATTAT 
     8941 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     9001 AATAAACAAA TAGGGGTCCC GCGCACATTT CCCCGAAAAG TGCCACCTGA C
//