LOCUS       RC204862                7518 bp    DNA     circular  SYN 
DEFINITION  MANBA (Myc-DDK-tagged)-Human mannosidase, beta A, lysosomal (MANBA)
ACCESSION   NM_005908
VERSION     NM_005908.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3675..3766 
                     /label="Myc-DDK"  
     misc_feature    3796..4381 
                     /label="PolyA signal"  
     misc_feature    3853..3872 
                     /label="Restriction Enzyme"  
     misc_feature    4657..5329 
                     /label="ColE1"  
     misc_feature    5759..6553 
                     /label="Kan/Neo"  
     misc_feature    6588..6890 
                     /label="SV40 ori"  
     misc_feature    6976..7453 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3657..3663 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3657 
                     /label="MANBA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCCTCCAC CTGCTCCTGC TGCTCGCGCT GTGCGGTGCA GGCACCACCG 
     1081 CCGCGGAGCT CAGTTACAGC TTGCGTGGCA ACTGGAGCAT CTGCAATGGG AACGGCTCGC 
     1141 TGGAGCTGCC CGGGGCGGTC CCTGGCTGCG TGCACAGCGC CTTGTTCCAG CAGGGCCTGA 
     1201 TCCAGGATTC TTACTACAGA TTTAATGACC TTAACTACAG ATGGGTCTCT TTGGATAACT 
     1261 GGACCTATAG CAAAGAATTT AAAATCCCCT TTGAAATTAG CAAATGGCAA AAAGTAAATT 
     1321 TGATTCTTGA GGGAGTGGAT ACGGTTTCAA AAATCCTGTT CAATGAAGTC ACTATTGGGG 
     1381 AAACAGACAA TATGTTCAAT AGATATAGCT TTGATATTAC CAACGTGGTC AGGGACGTGA 
     1441 ACTCCATTGA GCTGCGTTTC CAGTCAGCGG TGTTGTATGC AGCACAGCAG AGCAAAGCTC 
     1501 ACACTCGCTA CCAGGTTCCC CCAGACTGCC CTCCACTTGT GCAGAAGGGT GAATGCCATG 
     1561 TCAACTTTGT TCGGAAGGAG CAATGTTCCT TTAGTTGGGA CTGGGGGCCT TCCTTTCCTA 
     1621 CCCAGGGAAT CTGGAAAGAT GTTAGAATTG AAGCCTATAA TATTTGTCAC CTGAACTACT 
     1681 TCACATTTTC CCCAATATAT GATAAGAGTG CCCAGGAGTG GAATCTGGAA ATAGAGTCTA 
     1741 CATTTGATGT TGTCAGCTCA AAGCCAGTTG GTGGTCAAGT GATCGTAGCC ATCCCTAAGT 
     1801 TGCAAACACA ACAGACATAC AGCATTGAAC TTCAACCTGG GAAAAGGATT GTTGAGCTAT 
     1861 TTGTGAACAT TAGCAAGAAT ATTACTGTAG AAACTTGGTG GCCTCATGGA CATGGAAACC 
     1921 AGACTGGGTA CAACATGACT GTTCTTTTTG AACTGGATGG AGGCTTAAAT ATTGAAAAAT 
     1981 CAGCTAAGGT TTATTTTAGG ACAGTGGAAC TTATAGAAGA GCCTATAAAA GGGTCTCCTG 
     2041 GTTTGAGTTT CTATTTCAAA ATTAATGGAT TTCCCATATT TCTAAAAGGC TCAAACTGGA 
     2101 TCCCAGCAGA TTCATTCCAG GACCGAGTAA CCTCTGAGTT GTTACGGCTC CTTTTACAGT 
     2161 CTGTTGTGGA TGCTAATATG AATACTCTTC GGGTTTGGGG AGGAGGAATT TATGAGCAGG 
     2221 ATGAATTCTA TGAACTCTGT GATGAACTAG GAATAATGGT ATGGCAGGAT TTTATGTTTG 
     2281 CCTGTGCCCT TTATCCAACT GATCAGGGCT TCCTGGATTC AGTGACAGCA GAAGTTGCCT 
     2341 ACCAGATCAA GAGACTGAAA TCTCATCCTT CTATCATCAT ATGGAGTGGC AATAATGAAA 
     2401 ATGAGGAGGC GCTGATGATG AATTGGTATC ATATCAGTTT CACTGACCGG CCAATCTACA 
     2461 TCAAGGACTA TGTGACACTC TATGTGAAAA ACATCAGAGA GCTCGTACTG GCAGGAGACA 
     2521 AGAGTCGTCC TTTTATTACG TCCAGTCCTA CAAATGGGGC TGAAACTGTT GCAGAAGCCT 
     2581 GGGTCTCTCA AAACCCTAAT AGCAATTATT TTGGTGATGT ACATTTTTAT GACTATATCA 
     2641 GTGATTGCTG GAACTGGAAA GTTTTCCCAA AAGCTCGATT TGCATCTGAA TATGGATATC 
     2701 AGTCCTGGCC GTCCTTCAGT ACATTAGAAA AGGTCTCGTC TACAGAGGAC TGGTCTTTCA 
     2761 ATAGCAAGTT TTCACTTCAT CGACAACATC ACGAAGGTGG TAACAAACAA ATGCTTTATC 
     2821 AGGCTGGACT TCATTTCAAA CTCCCCCAAA GCACAGATCC ATTACGCACA TTTAAAGATA 
     2881 CCATCTACCT TACTCAGGTG ATGCAGGCCC AGTGTGTCAA AACAGAAACT GAATTCTACC 
     2941 GCCGTAGTCG CAGCGAGATA GTGGATCAGC AAGGGCACAC GATGGGGGCA CTTTATTGGC 
     3001 AGTTGAATGA CATCTGGCAA GCTCCTTCCT GGGCTTCTCT TGAGTACGGA GGAAAGTGGA 
     3061 AAATGCTTCA TTACTTTGCT CAGAATTTCT TTGCTCCACT GTTGCCAGTA GGCTTTGAGA 
     3121 ATGAAAACAT GTTCTATATC TATGGTGTGT CAGATCTTCA CTCGGATTAT TCGATGACAC 
     3181 TCAGTGTGAG AGTCCATACA TGGAGCTCCC TGGAGCCCGT GTGCTCTCGT GTGACTGAAC 
     3241 GTTTTGTGAT GAAAGGAGGA GAGGCTGTCT GCCTTTATGA GGAGCCAGTG TCTGAATTGC 
     3301 TGAGGAGATG TGGGAATTGC ACACGGGAAA GCTGTGTGGT TTCCTTTTAC CTTTCAGCTG 
     3361 ACCATGAACT CCTGAGCCCG ACCAACTACC ACTTCCTGTC CTCACCGAAG GAGGCCGTGG 
     3421 GGCTCTGCAA GGCGCAGATC ACTGCCATCA TCTCTCAGCA AGGTGACATA TTTGTTTTTG 
     3481 ACCTGGAGAC CTCAGCTGTC GCTCCCTTTG TTTGGTTGGA TGTAGGAAGC ATCCCAGGGA 
     3541 GATTTAGTGA CAATGGTTTC CTCATGACTG AGAAGACACG AACTATATTA TTTTACCCTT 
     3601 GGGAGCCCAC CAGCAAGAAT GAGTTGGAGC AATCTTTTCA TGTGACCTCC TTAACAGATA 
     3661 TTTACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3721 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3781 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3841 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3901 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3961 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4021 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4081 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4141 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4201 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4261 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4321 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4381 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4441 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4501 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4561 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4621 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4681 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4741 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4801 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4861 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4921 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4981 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5041 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5101 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5161 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5221 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5281 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5341 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5401 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5461 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5521 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5581 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5641 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5701 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5761 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5821 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5881 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5941 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6001 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6061 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6121 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6181 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6241 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6301 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6361 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6421 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6481 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6541 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6601 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6661 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6721 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6781 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6841 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6901 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6961 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7021 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7081 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7141 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7201 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7261 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7321 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7381 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7441 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7501 AAATTTAACG CGAATTTT
//