LOCUS       RC204837L2                9078 bp    DNA     circular  SYN 
DEFINITION  DHX58 (Myc-DDK-tagged)-Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58)
ACCESSION   NM_024119
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7367..7547 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8275..8934 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7595..8214 
                     /label="ColE1"  
     misc_feature    6242..6262 
                     /label="LR50"  
     misc_feature    5382..6107 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6251..6842 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5354..5361 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5354 
                     /label="DHX58"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGCTTCGG TCCTACCAAT GGGAGGTGAT 
     3361 CATGCCTGCC CTGGAGGGCA AGAATATCAT CATCTGGCTG CCCACGGGTG CCGGGAAGAC 
     3421 CCGGGCGGCT GCTTATGTGG CCAAGCGGCA CCTAGAGACT GTGGATGGAG CCAAGGTGGT 
     3481 TGTATTGGTC AACAGGGTGC ACCTGGTGAC CCAGCATGGT GAAGAGTTCA GGCGCATGCT 
     3541 GGATGGACGC TGGACCGTGA CAACCCTGAG TGGGGACATG GGACCACGTG CTGGCTTTGG 
     3601 CCACCTGGCC CGGTGCCATG ACCTGCTCAT CTGCACAGCA GAGCTTCTGC AGATGGCACT 
     3661 GACCAGCCCC GAGGAGGAGG AGCACGTGGA GCTCACTGTC TTCTCCCTGA TCGTGGTGGA 
     3721 TGAGTGCCAC CACACGCACA AGGACACCGT CTACAACGTC ATCATGAGCC AGTACCTAGA 
     3781 ACTTAAACTC CAGAGGGCAC AGCCGCTACC CCAGGTGCTG GGTCTCACAG CCTCCCCAGG 
     3841 CACTGGCGGG GCCTCCAAAC TCGATGGGGC CATCAACCAC GTCCTGCAGC TCTGTGCCAA 
     3901 CTTGGACACG TGGTGCATCA TGTCACCCCA GAACTGCTGC CCCCAGCTGC AGGAGCACAG 
     3961 CCAACAGCCT TGCAAACAGT ACAACCTCTG CCACAGGCGC AGCCAGGATC CGTTTGGGGA 
     4021 CTTGCTGAAG AAGCTCATGG ACCAAATCCA TGACCACCTG GAGATGCCTG AGTTGAGCCG 
     4081 GAAATTTGGG ACGCAAATGT ATGAGCAGCA GGTGGTGAAG CTGAGTGAGG CTGCGGCTTT 
     4141 GGCTGGGCTT CAGGAGCAAC GGGTGTATGC GCTTCACCTG AGGCGCTACA ATGACGCGCT 
     4201 GCTCATCCAT GACACCGTCC GCGCCGTGGA TGCCTTGGCT GCGCTGCAGG ATTTCTATCA 
     4261 CAGGGAGCAC GTCACTAAAA CCCAGATCCT GTGTGCCGAG CGCCGGCTGC TGGCCCTGTT 
     4321 CGATGACCGC AAGAATGAGC TGGCCCACTT GGCAACTCAT GGCCCAGAGA ATCCAAAACT 
     4381 GGAGATGCTG GAAAAGATCC TGCAAAGGCA GTTCAGTAGC TCTAACAGCC CTCGGGGTAT 
     4441 CATCTTCACC CGCACCCGCC AAAGCGCACA CTCCCTCCTG CTCTGGCTCC AGCAGCAGCA 
     4501 GGGCCTGCAG ACTGTGGACA TCCGGGCCCA GCTACTGATT GGGGCTGGGA ACAGCAGCCA 
     4561 GAGCACCCAC ATGACCCAGA GGGACCAGCA AGAAGTGATC CAGAAGTTCC AAGATGGAAC 
     4621 CCTGAACCTT CTGGTGGCCA CGAGTGTGGC GGAGGAGGGG CTGGACATCC CACATTGCAA 
     4681 TGTGGTGGTG CGTTATGGGC TCTTGACCAA TGAAATCTCC ATGGTCCAGG CCAGGGGCCG 
     4741 TGCCCGGGCC GATCAGAGTG TATACGCGTT TGTAGCAACT GAAGGTAGCC GGGAGCTGAA 
     4801 GCGGGAGCTG ATCAACGAGG CGCTGGAGAC GCTGATGGAG CAGGCAGTGG CTGCTGTGCA 
     4861 GAAAATGGAC CAGGCCGAGT ACCAGGCCAA GATCCGGGAT CTGCAGCAGG CAGCCTTGAC 
     4921 CAAGCGGGCG GCCCAGGCAG CCCAGCGGGA GAACCAGCGG CAGCAGTTCC CAGTGGAGCA 
     4981 CGTGCAGCTA CTCTGCATCA ACTGCATGGT GGCTGTGGGC CATGGCAGCG ACCTGCGGAA 
     5041 GGTGGAGGGC ACCCACCATG TCAATGTGAA CCCCAACTTC TCGAACTACT ATAATGTCTC 
     5101 CAGGGATCCT GTGGTCATCA ACAAAGTCTT CAAGGACTGG AAGCCTGGGG GTGTCATCAG 
     5161 CTGCAGGAAC TGTGGGGAGG TCTGGGGTCT GCAGATGATC TACAAGTCAG TGAAGCTGCC 
     5221 AGTGCTCAAA GTCCGCAGCA TGCTGCTGGA GACCCCTCAG GGGCGGATCC AGGCCAAAAA 
     5281 GTGGTCCCGC GTGCCCTTCT CCGTGCCTGA CTTTGACTTC CTGCAGCATT GTGCCGAGAA 
     5341 CTTGTCGGAC CTCTCCCTGG ACAGCGGACC GACGCGTACG CGGCCGCTCG AGATGAGCGG 
     5401 GGGCGAGGAG CTGTTCGCCG GCATCGTGCC CGTGCTGATC GAGCTGGACG GCGACGTGCA 
     5461 CGGCCACAAG TTCAGCGTGC GCGGCGAGGG CGAGGGCGAC GCCGACTACG GCAAGCTGGA 
     5521 GATCAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TGGTGACCAC 
     5581 CCTCTGCTAC GGCATCCAGT GCTTCGCCCG CTACCCCGAG CACATGAAGA TGAACGACTT 
     5641 CTTCAAGAGC GCCATGCCCG AGGGCTACAT CCAGGAGCGC ACCATCCAGT TCCAGGACGA 
     5701 CGGCAAGTAC AAGACCCGCG GCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT 
     5761 CGAGCTGAAG GGCAAGGACT TCAAGGAGGA CGGCAACATC CTGGGCCACA AGCTGGAGTA 
     5821 CAGCTTCAAC AGCCACAACG TGTACATCCG CCCCGACAAG GCCAACAACG GCCTGGAGGC 
     5881 TAACTTCAAG ACCCGCCACA ACATCGAGGG CGGCGGCGTG CAGCTGGCCG ACCACTACCA 
     5941 GACCAACGTG CCCCTGGGCG ACGGCCCCGT GCTGATCCCC ATCAACCACT ACCTGAGCAC 
     6001 TCAGACCAAG ATCAGCAAGG ACCGCAACGA GGCCCGCGAC CACATGGTGC TCCTGGAGTC 
     6061 CTTCAGCGCC TGCTGCCACA CCCACGGCAT GGACGAGCTG TACAGGTCCG GACTCAGATA 
     6121 AGTTTAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG TCGAGGGACC TAATAACTTC 
     6181 GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT TCCGGTTCCA CTAGGTACAA 
     6241 TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT 
     6301 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG 
     6361 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG 
     6421 CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG 
     6481 TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG 
     6541 ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC 
     6601 TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA 
     6661 TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC 
     6721 TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT 
     6781 CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC 
     6841 GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA GAAAAACATG GAGCAATCAC 
     6901 AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA 
     6961 GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC 
     7021 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA 
     7081 ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTG 
     7141 GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA 
     7201 GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT GAAGGAGAGA ACACCCGCTT 
     7261 GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTAT TAGAGTGGAG 
     7321 GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG ACTGTACTGG 
     7381 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 
     7441 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 
     7501 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 
     7561 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     7621 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     7681 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     7741 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     7801 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     7861 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     7921 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     7981 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     8041 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     8101 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     8161 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     8221 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     8281 CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG 
     8341 CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC 
     8401 TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA 
     8461 TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC 
     8521 ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT 
     8581 TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA 
     8641 AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC 
     8701 AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG CATTCATCAG GCGGGCAAGA 
     8761 ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC 
     8821 GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA 
     8881 AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC 
     8941 TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     9001 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     9061 CGAAAAGTGC CACCTGAC
//