LOCUS       RC204837                6924 bp    DNA     circular  SYN 
DEFINITION  DHX58 (Myc-DDK-tagged)-Human DEXH (Asp-Glu-X-His) box polypeptide 58 (DHX58)
ACCESSION   NM_024119
VERSION     NM_024119.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3079..3170 
                     /label="Myc-DDK"  
     misc_feature    3200..3785 
                     /label="PolyA signal"  
     misc_feature    3257..3276 
                     /label="Restriction Enzyme"  
     misc_feature    4061..4733 
                     /label="ColE1"  
     misc_feature    5163..5957 
                     /label="Kan/Neo"  
     misc_feature    5992..6294 
                     /label="SV40 ori"  
     misc_feature    6380..6857 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3054..3061 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3054 
                     /label="DHX58"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTTCGG TCCTACCAAT GGGAGGTGAT CATGCCTGCC CTGGAGGGCA 
     1081 AGAATATCAT CATCTGGCTG CCCACGGGTG CCGGGAAGAC CCGGGCGGCT GCTTATGTGG 
     1141 CCAAGCGGCA CCTAGAGACT GTGGATGGAG CCAAGGTGGT TGTATTGGTC AACAGGGTGC 
     1201 ACCTGGTGAC CCAGCATGGT GAAGAGTTCA GGCGCATGCT GGATGGACGC TGGACCGTGA 
     1261 CAACCCTGAG TGGGGACATG GGACCACGTG CTGGCTTTGG CCACCTGGCC CGGTGCCATG 
     1321 ACCTGCTCAT CTGCACAGCA GAGCTTCTGC AGATGGCACT GACCAGCCCC GAGGAGGAGG 
     1381 AGCACGTGGA GCTCACTGTC TTCTCCCTGA TCGTGGTGGA TGAGTGCCAC CACACGCACA 
     1441 AGGACACCGT CTACAACGTC ATCATGAGCC AGTACCTAGA ACTTAAACTC CAGAGGGCAC 
     1501 AGCCGCTACC CCAGGTGCTG GGTCTCACAG CCTCCCCAGG CACTGGCGGG GCCTCCAAAC 
     1561 TCGATGGGGC CATCAACCAC GTCCTGCAGC TCTGTGCCAA CTTGGACACG TGGTGCATCA 
     1621 TGTCACCCCA GAACTGCTGC CCCCAGCTGC AGGAGCACAG CCAACAGCCT TGCAAACAGT 
     1681 ACAACCTCTG CCACAGGCGC AGCCAGGATC CGTTTGGGGA CTTGCTGAAG AAGCTCATGG 
     1741 ACCAAATCCA TGACCACCTG GAGATGCCTG AGTTGAGCCG GAAATTTGGG ACGCAAATGT 
     1801 ATGAGCAGCA GGTGGTGAAG CTGAGTGAGG CTGCGGCTTT GGCTGGGCTT CAGGAGCAAC 
     1861 GGGTGTATGC GCTTCACCTG AGGCGCTACA ATGACGCGCT GCTCATCCAT GACACCGTCC 
     1921 GCGCCGTGGA TGCCTTGGCT GCGCTGCAGG ATTTCTATCA CAGGGAGCAC GTCACTAAAA 
     1981 CCCAGATCCT GTGTGCCGAG CGCCGGCTGC TGGCCCTGTT CGATGACCGC AAGAATGAGC 
     2041 TGGCCCACTT GGCAACTCAT GGCCCAGAGA ATCCAAAACT GGAGATGCTG GAAAAGATCC 
     2101 TGCAAAGGCA GTTCAGTAGC TCTAACAGCC CTCGGGGTAT CATCTTCACC CGCACCCGCC 
     2161 AAAGCGCACA CTCCCTCCTG CTCTGGCTCC AGCAGCAGCA GGGCCTGCAG ACTGTGGACA 
     2221 TCCGGGCCCA GCTACTGATT GGGGCTGGGA ACAGCAGCCA GAGCACCCAC ATGACCCAGA 
     2281 GGGACCAGCA AGAAGTGATC CAGAAGTTCC AAGATGGAAC CCTGAACCTT CTGGTGGCCA 
     2341 CGAGTGTGGC GGAGGAGGGG CTGGACATCC CACATTGCAA TGTGGTGGTG CGTTATGGGC 
     2401 TCTTGACCAA TGAAATCTCC ATGGTCCAGG CCAGGGGCCG TGCCCGGGCC GATCAGAGTG 
     2461 TATACGCGTT TGTAGCAACT GAAGGTAGCC GGGAGCTGAA GCGGGAGCTG ATCAACGAGG 
     2521 CGCTGGAGAC GCTGATGGAG CAGGCAGTGG CTGCTGTGCA GAAAATGGAC CAGGCCGAGT 
     2581 ACCAGGCCAA GATCCGGGAT CTGCAGCAGG CAGCCTTGAC CAAGCGGGCG GCCCAGGCAG 
     2641 CCCAGCGGGA GAACCAGCGG CAGCAGTTCC CAGTGGAGCA CGTGCAGCTA CTCTGCATCA 
     2701 ACTGCATGGT GGCTGTGGGC CATGGCAGCG ACCTGCGGAA GGTGGAGGGC ACCCACCATG 
     2761 TCAATGTGAA CCCCAACTTC TCGAACTACT ATAATGTCTC CAGGGATCCT GTGGTCATCA 
     2821 ACAAAGTCTT CAAGGACTGG AAGCCTGGGG GTGTCATCAG CTGCAGGAAC TGTGGGGAGG 
     2881 TCTGGGGTCT GCAGATGATC TACAAGTCAG TGAAGCTGCC AGTGCTCAAA GTCCGCAGCA 
     2941 TGCTGCTGGA GACCCCTCAG GGGCGGATCC AGGCCAAAAA GTGGTCCCGC GTGCCCTTCT 
     3001 CCGTGCCTGA CTTTGACTTC CTGCAGCATT GTGCCGAGAA CTTGTCGGAC CTCTCCCTGG 
     3061 ACAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3121 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3181 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3241 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3301 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3361 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3421 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3481 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3541 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3601 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3661 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3721 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3781 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3841 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3901 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3961 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4021 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4081 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4141 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4201 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4261 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4321 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4381 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4441 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4501 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4561 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4621 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4681 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4741 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4801 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4861 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4921 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4981 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5041 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5101 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5161 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5221 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5281 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5341 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5401 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5461 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5521 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5581 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5641 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5701 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5761 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5821 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5881 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5941 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6001 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6061 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6121 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6181 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6241 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6301 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6361 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6421 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6481 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6541 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6601 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6661 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6721 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6781 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6841 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6901 TAACAAAAAT TTAACGCGAA TTTT
//