LOCUS       RC204576                8079 bp    DNA     circular  SYN 
DEFINITION  VCL (Myc-DDK-tagged)-Human vinculin (VCL), transcript variant 2
ACCESSION   NM_003373
VERSION     NM_003373.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4236..4327 
                     /label="Myc-DDK"  
     misc_feature    4357..4942 
                     /label="PolyA signal"  
     misc_feature    4414..4433 
                     /label="Restriction Enzyme"  
     misc_feature    5218..5890 
                     /label="ColE1"  
     misc_feature    6320..7114 
                     /label="Kan/Neo"  
     misc_feature    7149..7451 
                     /label="SV40 ori"  
     misc_feature    7537..8014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4218 
                     /label="VCL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGTGTTT CATACGCGCA CGATCGAGAG CATCCTGGAG CCGGTGGCAC 
     1081 AGCAGATCTC CCACCTGGTG ATAATGCACG AGGAGGGCGA GGTGGACGGC AAAGCCATTC 
     1141 CTGACCTCAC CGCGCCCGTG GCCGCCGTGC AGGCGGCCGT CAGCAACCTC GTCCGGGTTG 
     1201 GAAAAGAGAC TGTTCAAACC ACTGAGGATC AGATTTTGAA GAGAGATATG CCACCAGCAT 
     1261 TTATTAAGGT TGAGAATGCT TGCACCAAGC TTGTCCAGGC AGCTCAGATG CTTCAGTCAG 
     1321 ACCCTTACTC AGTGCCTGCT CGAGATTATC TAATTGATGG GTCAAGGGGC ATCCTCTCTG 
     1381 GAACATCAGA CCTGCTCCTT ACCTTCGATG AGGCTGAGGT CCGTAAAATT ATTAGAGTTT 
     1441 GCAAAGGAAT TTTGGAATAT CTTACAGTGG CAGAGGTGGT GGAGACTATG GAAGATTTGG 
     1501 TCACTTACAC AAAGAATCTT GGGCCAGGAA TGACTAAGAT GGCCAAGATG ATTGACGAGA 
     1561 GACAGCAGGA GCTCACTCAC CAGGAGCACC GAGTGATGTT GGTGAACTCG ATGAACACCG 
     1621 TGAAAGAGTT GCTGCCAGTT CTCATTTCAG CTATGAAGAT TTTTGTAACA ACTAAAAACT 
     1681 CAAAAAACCA AGGCATAGAG GAAGCTTTAA AAAATCGCAA TTTTACTCTA GAAAAAATGA 
     1741 GTGCTGAAAT TAATGAGATA ATTCGTGTGT TACAACTCAC CTCTTGGGAT GAAGATGCCT 
     1801 GGGCCAGCAA GGACACTGAA GCCATGAAGA GAGCATTGGC CTCCATAGAC TCCAAACTGA 
     1861 ACCAGGCCAA AGGTTGGCTC CGTGACCCTA GTGCCTCCCC AGGGGATGCT GGTGAGCAGG 
     1921 CCATCAGACA GATCTTAGAT GAAGCTGGAA AAGTTGGTGA ACTCTGTGCA GGCAAAGAAC 
     1981 GCAGGGAGAT TCTGGGAACT TGCAAAATGC TAGGGCAGAT GACTGATCAA GTGGCTGACC 
     2041 TCCGTGCCAG AGGACAAGGA TCCTCACCGG TGGCCATGCA GAAAGCTCAG CAGGTATCTC 
     2101 AGGGTCTGGA TGTGCTCACA GCAAAAGTGG AAAATGCAGC TCGCAAGCTG GAAGCCATGA 
     2161 CCAACTCAAA GCAGAGCATT GCAAAGAAGA TCGATGCTGC TCAGAACTGG CTTGCAGATC 
     2221 CAAATGGTGG ACCGGAAGGA GAAGAGCAGA TTCGAGGTGC TTTGGCTGAA GCTCGGAAAA 
     2281 TAGCAGAATT ATGTGATGAT CCTAAAGAAA GAGATGACAT TCTACGTTCC CTTGGGGAAA 
     2341 TATCTGCTCT GACTTCTAAA TTAGCAGATC TACGAAGACA GGGGAAAGGA GATTCTCCAG 
     2401 AGGCTCGAGC CTTGGCCAAA CAGGTGGCCA CGGCCCTGCA GAACCTGCAG ACCAAAACCA 
     2461 ACCGGGCTGT GGCCAACAGC AGACCGGCCA AAGCAGCTGT ACACCTTGAG GGCAAGATTG 
     2521 AGCAAGCACA GCGGTGGATT GATAATCCCA CAGTGGATGA CCGTGGAGTC GGTCAGGCTG 
     2581 CCATCCGGGG GCTTGTGGCC GAAGGGCATC GTCTGGCTAA TGTTATGATG GGGCCTTATC 
     2641 GGCAAGATCT TCTCGCCAAG TGTGACCGAG TGGACCAGCT GACAGCCCAG CTGGCTGACC 
     2701 TGGCTGCCAG AGGGGAAGGG GAGAGTCCTC AGGCACGAGC ACTTGCATCT CAGCTCCAAG 
     2761 ACTCCTTAAA GGATCTAAAA GCTCGGATGC AGGAGGCCAT GACTCAGGAA GTGTCAGATG 
     2821 TTTTCAGCGA TACCACAACT CCCATCAAGC TGTTGGCAGT GGCAGCCACG GCGCCTCCTG 
     2881 ATGCGCCTAA CAGGGAAGAG GTATTTGATG AGAGGGCAGC TAACTTTGAA AACCATTCAG 
     2941 GAAAGCTTGG TGCTACGGCC GAGAAGGCGG CTGCGGTTGG TACTGCTAAT AAATCAACAG 
     3001 TGGAAGGCAT TCAGGCCTCA GTGAAGACGG CCCGAGAACT CACACCCCAG GTGGTCTCGG 
     3061 CTGCTCGTAT CTTACTTAGG AACCCTGGAA ATCAAGCTGC TTATGAACAT TTTGAGACCA 
     3121 TGAAGAACCA GTGGATCGAT AATGTTGAAA AAATGACAGG GCTGGTGGAC GAAGCCATTG 
     3181 ATACCAAATC TCTGTTGGAT GCTTCAGAAG AAGCAATTAA AAAAGACCTG GACAAGTGCA 
     3241 AGGTAGCTAT GGCCAACATT CAGCCTCAGA TGCTGGTTGC TGGGGCAACC AGTATTGCTC 
     3301 GTCGGGCCAA CCGGATCCTG CTGGTGGCTA AGAGGGAGGT GGAGAATTCC GAGGATCCCA 
     3361 AGTTCCGTGA GGCTGTGAAA GCTGCCTCTG ATGAATTGAG CAAAACCATC TCCCCGATGG 
     3421 TGATGGATGC AAAAGCTGTG GCTGGAAACA TTTCCGACCC TGGACTGCAA AAGAGCTTCC 
     3481 TGGACTCAGG ATATCGGATC CTGGGAGCTG TGGCCAAGGT CAGAGAAGCC TTCCAACCTC 
     3541 AGGAGCCTGA CTTCCCGCCG CCTCCACCAG ACCTTGAACA ACTCCGACTA ACAGATGAGC 
     3601 TTGCTCCTCC CAAACCACCT CTGCCTGAAG GTGAGGTCCC TCCACCTAGG CCTCCACCAC 
     3661 CAGAGGAAAA GGATGAAGAG TTCCCTGAGC AGAAGGCCGG GGAGGTGATT AACCAGCCAA 
     3721 TGATGATGGC TGCCAGACAG CTCCATGATG AAGCTCGCAA ATGGTCCAGC AAGGGCAATG 
     3781 ACATCATTGC AGCAGCCAAG CGCATGGCTC TGCTGATGGC TGAGATGTCT CGGCTGGTAA 
     3841 GAGGGGGCAG TGGTACCAAG CGGGCACTCA TTCAGTGTGC CAAGGACATC GCCAAGGCCT 
     3901 CAGATGAGGT GACTCGGTTG GCCAAGGAGG TTGCCAAGCA GTGCACAGAT AAACGGATTA 
     3961 GAACCAACCT CTTACAGGTA TGTGAGCGAA TCCCAACCAT AAGCACCCAG CTCAAAATCC 
     4021 TGTCCACAGT GAAGGCCACC ATGCTGGGCC GGACCAACAT CAGTGATGAG GAGTCTGAGC 
     4081 AGGCCACAGA GATGCTGGTT CACAATGCCC AGAACCTCAT GCAGTCTGTG AAGGAGACTG 
     4141 TGCGGGAAGC TGAAGCTGCT TCAATCAAAA TTCGAACAGA TGCTGGATTT ACACTGCGCT 
     4201 GGGTTAGAAA GACTCCCTGG TACCAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4261 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5881 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5941 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6001 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6061 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6121 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6181 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6241 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6301 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6361 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6421 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6481 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6541 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6601 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6661 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6721 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6781 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6841 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6901 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6961 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7021 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7081 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7141 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7201 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7261 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7321 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7501 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7561 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7621 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//