LOCUS       RC204467                8463 bp    DNA     circular  SYN 
DEFINITION  DHX30 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 30 (DHX30), transcript variant 1
ACCESSION   NM_138615
VERSION     NM_138615.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4620..4711 
                     /label="Myc-DDK"  
     misc_feature    4741..5326 
                     /label="PolyA signal"  
     misc_feature    4798..4817 
                     /label="Restriction Enzyme"  
     misc_feature    5602..6274 
                     /label="ColE1"  
     misc_feature    6704..7498 
                     /label="Kan/Neo"  
     misc_feature    7533..7835 
                     /label="SV40 ori"  
     misc_feature    7921..8398 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4602..4608 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4602 
                     /label="DHX30"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCAGCCTG GACTCATTCA GAAAAGATCG GGCCCAGCAC AGGCAGCGTC 
     1081 AGTGCAAACT TCCCCCACCC CGCCTTCCAC CCATGTGTGT CAACCCTACC CCAGGAGGGA 
     1141 CCATCTCTCG AGCTTCTAGG GACCTATTAA AAGAGTTCCC ACAGCCCAAA AATCTTCTCA 
     1201 ACAGTGTGAT TGGAAGAGCC CTCGGCATCT CACATGCAAA AGACAAACTA GTCTACGTGC 
     1261 ACACAAATGG ACCGAAGAAA AAGAAAGTCA CACTGCACAT AAAATGGCCC AAGAGCGTGG 
     1321 AGGTAGAAGG CTATGGCAGC AAGAAGATCG ATGCTGAGCG GCAGGCTGCA GCTGCAGCCT 
     1381 GCCAGCTGTT CAAGGGTTGG GGTCTGCTAG GTCCCCGGAA TGAGTTGTTT GACGCAGCCA 
     1441 AATACCGAGT GCTAGCTGAT CGCTTTGGCT CCCCTGCCGA CAGCTGGTGG CGTCCGGAAC 
     1501 CCACCATGCC CCCTACTTCC TGGCGGCAGC TGAATCCAGA GAGTATTCGA CCAGGGGGAC 
     1561 CTGGGGGCCT ATCCCGCTCT TTAGGCCGGG AAGAAGAGGA GGACGAGGAG GAAGAGCTAG 
     1621 AAGAAGGGAC CATAGATGTT ACCGACTTCT TGTCCATGAC CCAGCAGGAT TCCCACGCTC 
     1681 CACTCAGGGA CTCAAGGGGG AGTTCCTTTG AGATGACAGA TGACGACAGT GCCATTAGGG 
     1741 CTCTGACCCA GTTTCCACTT CCCAAGAACC TTCTGGCCAA GGTGATTCAG ATTGCAACGT 
     1801 CATCCTCCAC AGCTAAGAAC CTCATGCAGT TCCATACTGT GGGCACCAAG ACCAAGCTGT 
     1861 CTACACTCAC CCTGCTCTGG CCCTGCCCCA TGACCTTTGT TGCCAAAGGG CGCCGCAAAG 
     1921 CAGAGGCTGA GAATAAGGCG GCAGCCTTGG CCTGCAAGAA ACTGAAGAGC CTGGGCCTGG 
     1981 TGGACAGGAA CAACGAACCG CTTACACACG CCATGTATAA CCTGGCCTCT TTGCGTGAGC 
     2041 TGGGTGAGAC CCAGCGCCGA CCATGCACCA TCCAGGTGCC CGAGCCCATC CTCCGCAAGA 
     2101 TAGAGACCTT CCTGAACCAT TACCCTGTGG AGAGTTCATG GATCGCCCCA GAACTCCGGC 
     2161 TGCAGAGTGA TGACATCTTG CCCTTGGGCA AGGACTCAGG GCCTCTGAGT GACCCTATCA 
     2221 CAGGCAAGCC CTATGTGCCC CTGTTGGAAG CAGAGGAGGT ACGTCTCAGC CAGAGTCTGC 
     2281 TAGAACTGTG GCGGCGGCGA GGGCCGGTCT GGCAGGAGGC CCCCCAGCTA CCTGTGGACC 
     2341 CACATCGGGA CACCATCCTC AACGCCATTG AGCAGCACCC GGTGGTGGTC ATCTCTGGGG 
     2401 ACACGGGCTG TGGGAAGACC ACGCGCATCC CCCAGCTGTT GCTGGAGCGC TATGTGACCG 
     2461 AGGGCCGAGG TGCCCGCTGC AATGTTATCA TCACCCAACC TCGCCGCATC TCTGCTGTGT 
     2521 CTGTGGCACA GCGGGTCAGC CACGAACTGG GCCCCTCCCT GCGCCGGAAT GTGGGCTTCC 
     2581 AGGTGCGGTT GGAAAGTAAG CCCCCATCCC GAGGCGGGGC CCTGCTCTTC TGCACTGTGG 
     2641 GTATCCTGCT GCGTAAGCTG CAGAGCAACC CCAGCCTGGA GGGCGTGAGC CACGTCATCG 
     2701 TGGATGAGGT GCATGAGCGG GACGTGAACA CAGACTTTCT GCTGATCCTG CTCAAGGGCC 
     2761 TGCAGCGGCT CAACCCGGCC CTGCGGCTGG TGCTCATGAG TGCCACAGGG GACAATGAGC 
     2821 GCTTCTCCCG ATACTTTGGT GGCTGCCCCG TCATCAAGGT GCCTGGCTTC ATGTACCCAG 
     2881 TCAAGGAGCA CTACCTAGAG GACATCCTGG CCAAGTTGGG CAAGCACCAG TACCTGCACC 
     2941 GGCACCGGCA CCATGAGTCT GAGGATGAAT GCGCACTCGA TTTGGACCTT GTGACTGATC 
     3001 TGGTTCTGCA CATCGATGCT CGCGGGGAAC CAGGTGGGAT CCTGTGCTTC CTGCCTGGGT 
     3061 GGCAGGAGAT CAAAGGAGTG CAGCAGCGCC TCCAGGAGGC CCTGGGCATG CACGAGAGCA 
     3121 AGTACCTCAT CCTGCCAGTG CACTCCAACA TCCCCATGAT GGATCAGAAG GCCATATTCC 
     3181 AGCAGCCTCC AGTTGGGGTG CGCAAGATTG TCTTGGCCAC CAACATTGCT GAGACTTCCA 
     3241 TCACAATCAA TGACATCGTG CATGTGGTGG ACAGTGGGCT GCACAAGGAA GAACGCTATG 
     3301 ACCTGAAGAC CAAGGTGTCC TGCCTGGAGA CAGTGTGGGT ATCAAGAGCC AATGTGATCC 
     3361 AGCGCCGGGG CCGGGCGGGC CGCTGCCAGT CCGGCTTTGC CTACCACTTG TTCCCTCGAA 
     3421 GCCGGCTGGA GAAAATGGTC CCTTTCCAAG TGCCAGAGAT CCTGCGCACA CCTCTTGAGA 
     3481 ACCTGGTGCT GCAAGCGAAA ATCCACATGC CTGAGAAGAC GGCGGTGGAG TTCCTGTCCA 
     3541 AGGCTGTGGA CAGTCCAAAC ATCAAGGCAG TGGACGAGGC TGTGATCTTG CTCCAGGAGA 
     3601 TCGGGGTGCT GGACCAGCGG GAGTACCTGA CTACCCTGGG GCAGCGCCTG GCTCACATCT 
     3661 CCACCGACCC CCGGTTGGCC AAGGCCATTG TGTTGGCTGC CATCTTCCGT TGCCTGCACC 
     3721 CACTACTGGT GGTCGTTTCC TGCCTCACCC GGGACCCCTT CAGCAGCAGC CTACAGAACC 
     3781 GGGCAGAGGT GGACAAGGTG AAAGCACTGT TGAGCCATGA CAGCGGCAGT GACCACCTGG 
     3841 CCTTTGTGCG GGCTGTCGCC GGCTGGGAGG AGGTGCTGCG TTGGCAGGAC CGCAGCTCCC 
     3901 GGGAGAATTA CCTGGAGGAA AACCTGCTGT ACGCACCCAG CCTGCGCTTC ATCCACGGAC 
     3961 TCATCAAGCA GTTCTCAGAG AACATTTATG AGGCCTTCCT GGTGGGGAAG CCCTCGGACT 
     4021 GCACCCTGGC CTCCGCCCAG TGCAACGAGT ACAGTGAGGA GGAGGAGCTG GTGAAGGGCG 
     4081 TGCTGATGGC CGGCCTCTAC CCCAACCTCA TCCAGGTGAG GCAGGGCAAG GTCACCCGGC 
     4141 AGGGGAAGTT CAAGCCCAAC AGCGTCACAT ATAGGACCAA ATCAGGCAAC ATCCTGCTGC 
     4201 ACAAGTCGAC CATTAACAGG GAGGCCACAC GGTTACGGAG CCGATGGCTG ACGTATTTCA 
     4261 TGGCAGTCAA GTCCAATGGC AGCGTCTTCG TCCGGGACTC CTCTCAGGTG CACCCGCTAG 
     4321 CTGTGCTGCT GCTGACCGAC GGGGACGTGC ACATCCGTGA TGACGGGCGC CGGGCCACCA 
     4381 TCTCACTGAG CGACAGTGAC CTGCTGCGGC TGGAGGGTGA CTCGCGTACC GTGCGGCTGC 
     4441 TGAAGGAGCT GCGGCGGGCC CTGGGCCGCA TGGTGGAGCG GAGCCTGCGC AGCGAGCTGG 
     4501 CTGCACTTCC CCCCAGCGTA CAGGAGGAGC ACGGGCAGCT GCTTGCGCTA CTGGCAGAGC 
     4561 TGCTGCGAGG ACCCTGTGGC AGCTTTGATG TGCGCAAGAC AGCTGACGAC ACGCGTACGC 
     4621 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4681 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4741 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4801 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4861 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4921 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4981 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5041 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5101 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5161 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5221 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5281 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5341 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5401 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5461 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5521 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5581 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5641 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5701 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5761 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5821 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5881 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5941 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6001 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6061 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6121 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6181 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6241 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6301 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6361 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6421 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6481 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6541 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6601 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6661 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6721 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6781 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6841 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6901 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6961 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7021 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7081 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7141 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7201 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7261 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7321 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7381 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7441 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7501 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7561 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7621 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7681 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7861 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7921 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7981 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8041 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8101 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8161 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8221 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8281 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8341 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8401 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8461 TTT
//