LOCUS       RC204388                7593 bp    DNA     circular  SYN 
DEFINITION  MTBP (Myc-DDK-tagged)-Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP)
ACCESSION   NM_022045
VERSION     NM_022045.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3750..3841 
                     /label="Myc-DDK"  
     misc_feature    3871..4456 
                     /label="PolyA signal"  
     misc_feature    3928..3947 
                     /label="Restriction Enzyme"  
     misc_feature    4732..5404 
                     /label="ColE1"  
     misc_feature    5834..6628 
                     /label="Kan/Neo"  
     misc_feature    6663..6965 
                     /label="SV40 ori"  
     misc_feature    7051..7528 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3732..3738 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3732 
                     /label="MTBP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCGGTAC CTGCTGCTGG TGATCTGGGG GGAAGGAAAA TTCCCGTCGG 
     1081 CGGCCAGTAG GGAGGCAGAA CATGGGCCAG AGGTGTCGTC GGGTGAGGGT ACTGAGAATC 
     1141 AGCCGGACTT CACAGCAGCA AATGTTTATC ACCTCTTGAA AAGAAGCATT AGTGCTTCAA 
     1201 TTAATCCAGA AGATAGTACT TTCCCTGCCT GTTCAGTGGG AGGTATACCT GGTTCCAAGA 
     1261 AGTGGTTCTT TGCAGTGCAG GCAATATATG GATTTTATCA GTTTTGTAGT TCTGATTGGC 
     1321 AAGAGATACA TTTTGATACA GAAAAAGATA AAATTGAAGA TGTTCTTCAA ACGAATATCG 
     1381 AAGAATGTTT GGGTGCTGTT GAGTGTTTTG AAGAAGAAGA CAGTAATAGC AGGGAATCAT 
     1441 TATCCTTGGC TGATCTCTAT GAAGAAGCTG CAGAAAATTT GCATCAGCTG TCAGACAAGC 
     1501 TTCCTGCTCC TGGTAGAGCA ATGGTAGATA TAATACTGTT GCTTTCTGAC AAAGATCCTC 
     1561 CTAAATTGAA AGACTATTTA CCTACTGTAG GAGCATTAAA ACATTTGAGA GAATGGTATT 
     1621 CAGCAAAGAT CACTATAGCA GGAAATCATT GTGAAATAAA CTGTCAGAAA ATTGCAGAAT 
     1681 ACCTTTCTGC TAATGTTGTA TCTTTAGAAG ATCTCAGAAA TGTTATTGAC TCAAAGGAAT 
     1741 TATGGAGGGG GAAAATACAG ATATGGGAAA GAAAGTTTGG ATTTGAAATT AGTTTTCCTG 
     1801 AATTTTGTTT AAAGGGAGTC ACACTTAAGA ATTTTAGTAC TTCTAATTTA AATACTGACT 
     1861 TCCTTGCCAA AAAGATCATA CCATCAAAGG ATAAGAATAT TTTGCCAAAG GTTTTCCATT 
     1921 ATTATGGCCC TGCTTTAGAA TTTGTGCAGA TGATAAAATT ATCAGATCTA CCCTCCTGCT 
     1981 ATATGTCGGA TATTGAATTT GAGTTAGGAT TGACAAACAG TACCAAACAG AATTCTGTGT 
     2041 TGCTGTTGGA GCAGATTTCT TCTCTGTGTA GCAAGGTTGG TGCTCTTTTT GTATTGCCAT 
     2101 GTACCATTAG TAACATACTG ATTCCACCTC CCAACCAACT CAGTTCAAGA AAATGGAAGG 
     2161 AATATATAGC TAAAAAGCCT AAAACAATCA GTGTTCCAGA TGTTGAAGTG AAAGGAGAGT 
     2221 GTTCTAGCTA TTATCTCTTG TTACAAGGTA ATGGCAATAG AAGATGTAAA GCCACATTGA 
     2281 TTCACTCAGC CAACCAGATC AATGGCTCAT TTGCACTCAA TTTAATTCAT GGAAAGATGA 
     2341 AAACAAAGAC AGAAGAAGCC AAATTGAGTT TTCCTTTTGA CTTATTATCA CTTCCACATT 
     2401 TTTCTGGGGA GCAGATTGTA CAGAGAGAGA AACAGTTAGC TAATGTTCAA GTTTTAGCTT 
     2461 TGGAAGAATG CCTAAAAAGA CGAAAGTTGG CAAAGCAGCC TGAAACAGTT TCTGTTGCTG 
     2521 AACTCAAAAG TCTGTTAGTA CTCACAAGGA AACACTTTTT AGATTATTTT GATGCTGTGA 
     2581 TTCCTAAAAT GATTCTAAGA AAGATGGACA AAATTAAAAC CTTCAATATA TTAAATGATT 
     2641 TTAGTCCAGT GGAACCTAAT TCCTCAAGTC TAATGGAAAC CAATCCTCTG GAATGGCCAG 
     2701 AAAGGCATGT TCTTCAAAAT TTGGAAACTT TTGAAAAAAC TAAACAAAAA ATGAGAACTG 
     2761 GTTCATTACC TCATTCATCT GAACAGTTGC TGGGCCACAA AGAGGGTCCT CGGGACTCAA 
     2821 TCACATTGTT GGATGCTAAA GAATTGCTGA AGTACTTTAC CTCAGATGGA TTACCCATTG 
     2881 GAGATCTTCA ACCTTTACCG ATTCAAAAGG GGGAAAAGAC TTTTGTTTTG ACACCAGAAC 
     2941 TTAGTCCTGG GAAACTTCAG GTCTTACCTT TTGAGAAAGC CTCAGTATGT CATTATCATG 
     3001 GAATTGAATA TTGCTTGGAT GACCGAAAAG CTTTGGAAAG AGATGGAGGA TTTTCTGAAC 
     3061 TTCAGTCTCG TCTTATTCGT TATGAAACTC AAACTACCTG CACCAGAGAA AGTTTTCCAG 
     3121 TACCTACTGT GTTGAGCCCT CTTCCATCTC CTGTAGTTTC GTCAGATCCT GGAAGTGTCC 
     3181 CTGACGGAGA AGTTTTACAA AATGAACTTC GAACTGAAGT ATCCCGATTG AAACGGAGAT 
     3241 CTAAAGATCT GAATTGCCTT TATCCCAGAA AAAGACTTGT GAAATCTGAA AGTTCAGAGT 
     3301 CTCTTCTTTC TCAGACAACT GGTAATAGTA ATCACTATCA TCATCATGTG ACATCCAGAA 
     3361 AGCCACAAAC AGAACGGTCC TTACCAGTGA CTTGTCCATT GGTTCCAATT CCTAGCTGTG 
     3421 AAACTCCAAA ACTTGCTACA AAGACCAGTT CAGGTCAAAA AAGTATGCAT GAATCAAAAA 
     3481 CATCAAGGCA AATTAAGGAA TCAAGATCAC AGAAACACAC ACGGATACTG AAAGAAGTAG 
     3541 TTACTGAAAC CCTGAAGAAA CACAGTATTA CCGAGACTCA TGAATGTTTC ACTGCATGCA 
     3601 GCCAGCGTCT CTTTGAAATC TCTAAGTTCT ATCTAAAGGA TCTTAAAACT TCAAGGGGTC 
     3661 TATTTGAAGA AATGAAGAAA ACAGCAAACA ACAATGCTGT ACAGGTGATT GACTGGGTAT 
     3721 TAGAAAAGAC AAGCAAGAAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3781 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3841 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3901 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3961 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4021 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4081 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4141 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4201 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4261 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4321 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4381 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4441 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4501 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4561 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4621 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4681 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4741 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4801 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4861 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4921 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4981 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5041 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5101 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5161 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5221 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5281 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5341 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5401 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5461 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5521 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5581 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5641 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5701 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5761 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5821 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5881 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5941 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6001 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6061 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6121 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6181 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6241 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6301 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6361 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6421 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6481 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6541 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6601 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6661 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6721 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6781 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6841 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6961 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7021 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7141 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7201 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7261 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7321 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7381 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7441 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7501 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7561 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//