LOCUS       RC204368                6726 bp    DNA     circular  SYN 
DEFINITION  ACSF2 (Myc-DDK-tagged)-Human acyl-CoA synthetase family member 2 (ACSF2)
ACCESSION   NM_025149
VERSION     NM_025149.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2883..2974 
                     /label="Myc-DDK"  
     misc_feature    3004..3589 
                     /label="PolyA signal"  
     misc_feature    3061..3080 
                     /label="Restriction Enzyme"  
     misc_feature    3865..4537 
                     /label="ColE1"  
     misc_feature    4967..5761 
                     /label="Kan/Neo"  
     misc_feature    5796..6098 
                     /label="SV40 ori"  
     misc_feature    6184..6661 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2865..2871 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2865 
                     /label="ACSF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTCTAC GTCGGGATGC TGCGCCTGGG GAGGCTGTGC GCCGGGAGCT 
     1081 CGGGGGTGCT GGGGGCCCGG GCCGCCCTCT CTCGGAGTTG GCAGGAAGCC AGGTTGCAGG 
     1141 GTGTCCGCTT CCTCAGTTCC AGAGAGGTGG ATCGCATGGT CTCCACGCCC ATCGGAGGCC 
     1201 TCAGCTACGT TCAGGGGTGC ACCAAAAAGC ATCTTAACAG CAAGACTGTG GTCCAGTGCC 
     1261 TGGAGACCAC AGCACAGAGG GTCCCAGAAC GAGAGGCCTT GGTCGTCCTC CATGAAGACG 
     1321 TCAGGTTGAC CTTTGCCCAA CTCAAGGAGG AGGTGGACAA AGCTGCTTCT GGCCTCCTGA 
     1381 GCATTGGCCT CTGCAAAGGT GACCGGCTGG GCATGTGGGG ACCTAACTCC TATGCATGGG 
     1441 TGCTCATGCA GTTGGCCACC GCCCAGGCGG GCATCATTCT GGTGTCTGTG AACCCAGCCT 
     1501 ACCAGGCTAT GGAACTGGAG TATGTCCTCA AGAAGGTGGG CTGCAAGGCC CTTGTGTTCC 
     1561 CCAAGCAATT CAAGACCCAG CAATACTACA ACGTCCTGAA GCAGATCTGT CCAGAAGTGG 
     1621 AGAATGCCCA GCCAGGGGCC TTGAAGAGTC AGAGGCTCCC AGATCTGACC ACAGTCATCT 
     1681 CGGTGGATGC CCCTTTGCCG GGGACCCTGC TCCTGGATGA AGTGGTGGCG GCTGGCAGCA 
     1741 CACGGCAGCA TCTGGACCAG CTCCAATACA ACCAGCAGTT CCTGTCCTGC CATGACCCCA 
     1801 TCAACATCCA GTTCACCTCG GGGACAACAG GCAGCCCCAA GGGGGCCACC CTCTCCCACT 
     1861 ACAACATTGT CAACAACTCC AACATTTTAG GAGAGCGCCT GAAACTGCAT GAGAAGACAC 
     1921 CAGAGCAGTT GCGGATGATC CTGCCCAACC CCCTGTACCA TTGCCTGGGT TCCGTGGCAG 
     1981 GCACAATGAT GTGTCTGATG TACGGTGCCA CCCTCATCCT GGCCTCTCCC ATCTTCAATG 
     2041 GCAAGAAGGC ACTGGAGGCC ATCAGCAGAG AGAGAGGCAC CTTCCTGTAT GGTACCCCCA 
     2101 CGATGTTCGT GGACATTCTG AACCAGCCAG ACTTCTCCAG TTATGACATC TCGACCATGT 
     2161 GTGGAGGTGT CATTGCTGGG TCCCCTGCAC CTCCAGAGTT GATCCGAGCC ATCATCAACA 
     2221 AGATAAATAT GAAGGACCTG GTGGTTGCTT ATGGAACCAC AGAGAACAGT CCCGTGACAT 
     2281 TCGCGCACTT CCCTGAGGAC ACTGTGGAGC AGAAGGCAGA AAGCGTGGGC AGAATTATGC 
     2341 CTCACACGGA GGCGCGGATC ATGAACATGG AGGCAGGGAC GCTGGCAAAG CTGAACACGC 
     2401 CCGGGGAGCT GTGCATCCGA GGGTACTGCG TCATGCTGGG CTACTGGGGT GAGCCTCAGA 
     2461 AGACAGAGGA AGCAGTGGAT CAGGACAAGT GGTATTGGAC AGGAGATGTC GCCACAATGA 
     2521 ATGAGCAGGG CTTCTGCAAG ATCGTGGGCC GCTCTAAGGA TATGATCATC CGGGGTGGTG 
     2581 AGAACATCTA CCCCGCAGAG CTCGAGGACT TCTTTCACAC ACACCCGAAG GTGCAGGAAG 
     2641 TGCAGGTGGT GGGAGTGAAG GACGATCGGA TGGGGGAAGA GATTTGTGCC TGCATTCGGC 
     2701 TGAAGGACGG GGAGGAGACC ACGGTGGAGG AGATAAAAGC TTTCTGCAAA GGGAAGATCT 
     2761 CTCACTTCAA GATTCCGAAG TACATCGTGT TTGTCACAAA CTACCCCCTC ACCATTTCAG 
     2821 GAAAGATCCA GAAATTCAAA CTTCGAGAGC AGATGGAACG ACATCTAAAT CTGACGCGTA 
     2881 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     2941 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3001 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3061 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3121 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3181 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3241 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3301 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3361 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3421 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     3481 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     3541 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     3601 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     3661 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     3721 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     3781 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     3841 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     3901 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     3961 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4021 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4081 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4141 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4201 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4261 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4321 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4381 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4441 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     4501 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     4561 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     4621 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     4681 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     4741 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     4801 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     4861 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     4921 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     4981 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5041 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5101 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5161 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5221 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5281 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5341 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5401 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     5461 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     5521 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     5581 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     5641 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     5701 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     5761 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     5821 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     5881 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     5941 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6001 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6061 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6121 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6181 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6241 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6301 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6361 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6421 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     6481 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     6541 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     6601 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     6661 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     6721 AATTTT
//