LOCUS       RC204351                8442 bp    DNA     circular  SYN 
DEFINITION  TYK2 (Myc-DDK-tagged)-Human tyrosine kinase 2 (TYK2)
ACCESSION   NM_003331
VERSION     NM_003331.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4599..4690 
                     /label="Myc-DDK"  
     misc_feature    4720..5305 
                     /label="PolyA signal"  
     misc_feature    4777..4796 
                     /label="Restriction Enzyme"  
     misc_feature    5581..6253 
                     /label="ColE1"  
     misc_feature    6683..7477 
                     /label="Kan/Neo"  
     misc_feature    7512..7814 
                     /label="SV40 ori"  
     misc_feature    7900..8377 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4581..4587 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4581 
                     /label="TYK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCTGCGC CACTGGGGGA TGGCCAGGGG CAGTAAGCCC GTTGGGGATG 
     1081 GAGCCCAGCC CATGGCTGCC ATGGGAGGCC TGAAGGTGCT TCTGCACTGG GCTGGTCCAG 
     1141 GCGGCGGGGA GCCCTGGGTC ACTTTCAGTG AGTCATCGCT GACAGCTGAG GAAGTCTGCA 
     1201 TCCACATTGC ACATAAAGTT GGTATCACTC CTCCTTGCTT CAATCTCTTT GCCCTCTTCG 
     1261 ATGCTCAGGC CCAAGTCTGG TTGCCCCCAA ACCACATCCT AGAGATCCCC AGAGATGCAA 
     1321 GCCTGATGCT ATATTTCCGC ATAAGGTTTT ATTTCCGGAA CTGGCATGGC ATGAATCCTC 
     1381 GGGAACCGGC TGTGTACCGT TGTGGGCCCC CAGGAACCGA GGCATCCTCA GATCAGACAG 
     1441 CACAGGGGAT GCAACTCCTG GACCCAGCCT CATTTGAGTA CCTCTTTGAG CAGGGCAAGC 
     1501 ATGAGTTTGT GAATGACGTG GCATCACTGT GGGAGCTGTC GACCGAGGAG GAGATCCACC 
     1561 ACTTTAAGAA TGAGAGCCTG GGCATGGCCT TTCTGCACCT CTGTCACCTC GCTCTCCGCC 
     1621 ATGGCATCCC CCTGGAGGAG GTGGCCAAGA AGACCAGCTT CAAGGACTGC ATCCCGCGCT 
     1681 CCTTCCGCCG GCATATCCGG CAGCACAGCG CCCTGACCCG GCTGCGCCTT CGGAACGTCT 
     1741 TCCGCAGGTT CCTGCGGGAC TTCCAGCCGG GCCGACTCTC CCAGCAGATG GTCATGGTCA 
     1801 AATACCTAGC CACACTCGAG CGGCTGGCAC CCCGCTTCGG CACAGAGCGT GTGCCCGTGT 
     1861 GCCACCTGAG GCTGCTGGCC CAGGCCGAGG GGGAGCCCTG CTACATCCGG GACAGTGGGG 
     1921 TGGCCCCTAC AGACCCTGGC CCTGAGTCTG CTGCTGGGCC CCCAACCCAC GAGGTGCTGG 
     1981 TGACAGGCAC TGGTGGCATC CAGTGGTGGC CAGTAGAGGA GGAGGTGAAC AAGGAGGAGG 
     2041 GTTCTAGTGG CAGCAGTGGC AGGAACCCCC AAGCCAGCCT GTTTGGGAAG AAGGCCAAGG 
     2101 CTCACAAGGC AGTCGGCCAG CCGGCAGACA GGCCGCGGGA GCCACTGTGG GCCTACTTCT 
     2161 GTGACTTCCG GGACATCACC CACGTGGTGC TGAAAGAGCA CTGTGTCAGC ATCCACCGGC 
     2221 AGGACAACAA GTGCCTGGAG CTGAGCTTGC CTTCCCGGGC TGCGGCGCTG TCCTTCGTGT 
     2281 CGCTGGTGGA CGGCTATTTC CGCCTGACGG CCGACTCCAG CCACTACCTG TGCCACGAGG 
     2341 TGGCTCCCCC ACGGCTGGTG ATGAGCATCC GGGATGGGAT CCACGGACCC CTGCTGGAGC 
     2401 CATTTGTGCA GGCCAAGCTG CGGCCCGAGG ACGGCCTGTA CCTCATTCAC TGGAGCACCA 
     2461 GCCACCCCTA CCGCCTGATC CTCACAGTGG CCCAGCGTAG CCAGGCACCA GACGGCATGC 
     2521 AGAGCTTGCG GCTCCGAAAG TTCCCCATTG AGCAGCAGGA CGGGGCCTTC GTGCTGGAGG 
     2581 GCTGGGGCCG GTCCTTCCCC AGCGTTCGGG AACTTGGGGC TGCCTTGCAG GGCTGCTTGC 
     2641 TGAGGGCCGG GGATGACTGC TTCTCTCTGC GTCGCTGTTG CCTGCCCCAA CCAGGAGAAA 
     2701 CCTCCAATCT CATCATCATG CGGGGGGCTC GGGCCAGCCC CAGGACACTC AACCTCAGCC 
     2761 AGCTCAGCTT CCACCGGGTT GACCAGAAGG AGATCACCCA GCTGTCCCAC TTGGGCCAGG 
     2821 GCACAAGGAC CAACGTGTAT GAGGGCCGCC TGCGAGTGGA GGGCAGCGGG GACCCTGAGG 
     2881 AGGGCAAGAT GGATGACGAG GACCCCCTCG TGCCTGGCAG GGACCGTGGG CAGGAGCTAC 
     2941 GAGTGGTGCT CAAAGTGCTG GACCCTAGTC ACCATGACAT CGCCCTGGCC TTCTACGAGA 
     3001 CAGCCAGCCT CATGAGCCAG GTCTCCCACA CGCACCTGGC CTTCGTGCAT GGCGTCTGTG 
     3061 TGCGCGGCCC TGAAAATATC ATGGTGACAG AGTACGTGGA GCACGGACCC CTGGATGTGT 
     3121 GGCTGCGGAG GGAGCGGGGC CATGTGCCCA TGGCTTGGAA GATGGTGGTG GCCCAGCAGC 
     3181 TGGCCAGCGC CCTCAGCTAC CTGGAGAACA AGAACCTGGT TCATGGTAAT GTGTGTGGCC 
     3241 GGAACATCCT GCTGGCCCGG CTGGGGTTGG CAGAGGGCAC CAGCCCCTTC ATCAAGCTGA 
     3301 GTGATCCTGG CGTGGGCCTG GGCGCCCTCT CCAGGGAGGA GCGGGTGGAG AGGATCCCCT 
     3361 GGCTGGCCCC CGAATGCCTA CCAGGTGGGG CCAACAGCCT AAGCACCGCC ATGGACAAGT 
     3421 GGGGGTTTGG CGCCACCCTC CTGGAGATCT GCTTTGACGG AGAGGCCCCT CTGCAGAGCC 
     3481 GCAGTCCCTC CGAGAAGGAG CATTTCTACC AGAGGCAGCA CCGGCTGCCC GAGCCCTCCT 
     3541 GCCCACAGCT GGCCACACTC ACCAGCCAGT GTCTGACCTA TGAGCCAACC CAGAGGCCAT 
     3601 CATTCCGCAC CATCCTGCGT GACCTCACCC GGCTGCAGCC CCACAATCTT GCTGACGTCT 
     3661 TGACTGTGAA CCCGGACTCA CCGGCGTCGG ACCCTACGGT TTTCCACAAG CGCTATTTGA 
     3721 AAAAGATCCG AGATCTGGGC GAGGGTCACT TCGGCAAGGT CAGCTTGTAC TGCTACGATC 
     3781 CGACCAACGA CGGCACTGGC GAGATGGTGG CGGTGAAAGC CCTCAAGGCA GACTGCGGCC 
     3841 CCCAGCACCG CTCGGGCTGG AAGCAGGAGA TTGACATTCT GCGCACGCTC TACCACGAGC 
     3901 ACATCATCAA GTACAAGGGC TGCTGCGAGG ACCAAGGCGA GAAGTCGCTG CAGCTGGTCA 
     3961 TGGAGTACGT GCCCCTGGGC AGCCTCCGAG ACTACCTGCC CCGGCACAGC ATCGGGCTGG 
     4021 CCCAGCTGCT GCTCTTCGCC CAGCAGATCT GCGAGGGCAT GGCCTATCTG CACTCGCAGC 
     4081 ACTACATCCA CCGAGACCTA GCCGCGCGCA ACGTGCTGCT GGACAACGAC AGGCTGGTCA 
     4141 AGATCGGGGA CTTTGGCCTA GCCAAGGCCG TGCCCGAAGG CCACGAGTAC TACCGCGTGC 
     4201 GCGAGGATGG GGACAGCCCC GTGTTCTGGT ATGCCCCAGA GTGCCTGAAG GAGTATAAGT 
     4261 TCTACTATGC GTCAGATGTC TGGTCCTTCG GGGTGACCCT GTATGAGCTG CTGACGCACT 
     4321 GTGACTCCAG CCAGAGCCCC CCCACGAAAT TCCTTGAGCT CATAGGCATT GCTCAGGGTC 
     4381 AGATGACAGT TCTGAGACTC ACTGAGTTGC TGGAACGAGG GGAGAGGCTG CCACGGCCCG 
     4441 ACAAATGTCC CTGTGAGGTC TATCATCTCA TGAAGAACTG CTGGGAGACA GAGGCGTCCT 
     4501 TTCGCCCAAC CTTCGAGAAC CTCATACCCA TTCTGAAGAC AGTCCATGAG AAGTACCAAG 
     4561 GCCAGGCCCC TTCAGTGTTC AGCGTGTGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4621 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4681 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4741 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4801 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4861 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4921 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4981 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5041 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5101 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5161 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5221 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5281 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5341 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5401 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5461 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5521 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5581 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5641 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5701 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5761 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5821 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5881 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5941 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6001 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6061 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6121 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6181 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6241 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6301 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6361 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6421 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6481 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6541 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6601 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6661 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6721 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6781 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6841 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6901 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6961 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7021 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7081 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7141 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7201 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7261 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7321 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7381 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7441 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7501 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7561 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7621 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7681 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7861 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7921 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7981 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8041 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8101 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8161 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8221 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8281 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8341 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8401 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//