LOCUS       RC204249                8241 bp    DNA     circular  SYN 
DEFINITION  NFX1 (Myc-DDK-tagged)-Human nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1
ACCESSION   NM_002504
VERSION     NM_002504.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4398..4489 
                     /label="Myc-DDK"  
     misc_feature    4519..5104 
                     /label="PolyA signal"  
     misc_feature    4576..4595 
                     /label="Restriction Enzyme"  
     misc_feature    5380..6052 
                     /label="ColE1"  
     misc_feature    6482..7276 
                     /label="Kan/Neo"  
     misc_feature    7311..7613 
                     /label="SV40 ori"  
     misc_feature    7699..8176 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4380..4386 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4380 
                     /label="NFX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGGCG CCTCCTGTCT CAGGTACTTT TAAATTCAAT ACAGATGCTG 
     1081 CTGAATTCAT TCCTCAGGAG AAAAAAAATT CTGGTCTAAA TTGTGGGACT CAAAGGAGAC 
     1141 TAGACTCTAA TAGGATTGGT AGAAGAAATT ACAGTTCACC ACCTCCCTGT CACCTTTCCA 
     1201 GGCAGGTCCC TTATGATGAA ATCTCTGCTG TTCATCAGCA TAGTTATCAT CCGTCAGGAA 
     1261 GCAAACCTAA GAGTCAGCAG ACGTCTTTCC AGTCCTCTCC TTGTAATAAA TCGCCCAAGA 
     1321 GCCATGGCCT TCAGAATCAA CCTTGGCAGA AATTGAGGAA TGAGAAGCAC CATATCAGAG 
     1381 TCAAGAAAGC ACAGAGTCTT GCTGAGCAGA CCTCAGATAC AGCTGGATTA GAGAGCTCGA 
     1441 CCAGATCAGA GAGTGGGACA GACCTCAGAG AGCATAGTCC TTCTGAGAGT GAGAAGGAAG 
     1501 TTGTGGGTGC AGATCCCAGG GGAGCAAAAC CCAAAAAAGC AACACAGTTT GTATACAGCT 
     1561 ATGGTAGAGG ACCAAAAGTC AAGGGGAAAC TCAAATGTGA ATGGAGTAAC CGAACAACTC 
     1621 CAAAACCGGA GGATGCTGGA CCCGAAAGTA CCAAACCTGT GGGGGTTTTC CACCCTGACT 
     1681 CTTCAGAGGC ATCCTCTAGA AAAGGAGTAT TGGATGGGTA TGGAGCCAGA CGAAATGAGC 
     1741 AGAGAAGATA CCCACAGAAA AGGCCTCCCT GGGAAGTGGA GGGGGCCAGG CCACGACCAG 
     1801 GCAGAAATCC ACCAAAACAG GAGGGCCACC GACATACAAA CGCAGGACAC AGAAACAACA 
     1861 TGGGCCCCAT TCCAAAGGAT GACCTCAATG AAAGACCAGC AAAATCTACC TGTGACAGTG 
     1921 AGAACTTGGC AGTCATCAAC AAGTCTTCCA GGAGGGTTGA CCAAGAGAAA TGCACTGTAC 
     1981 GGAGGCAGGA TCCTCAAGTA GTATCTCCTT TCTCCCGAGG CAAACAGAAC CATGTGCTAA 
     2041 AGAATGTGGA AACGCACACA GGTTCTCTAA TTGAACAACT AACAACAGAA AAATACGAGT 
     2101 GCATGGTGTG CTGTGAATTG GTTCGTGTCA CGGCCCCAGT GTGGAGTTGT CAGAGCTGTT 
     2161 ACCATGTGTT TCATTTGAAC TGCATAAAGA AATGGGCAAG GTCTCCAGCA TCTCAAGCAG 
     2221 ATGGCCAGAG TGGTTGGAGG TGCCCTGCCT GTCAGAATGT TTCTGCACAT GTTCCTAATA 
     2281 CCTACACTTG TTTCTGTGGC AAGGTAAAGA ATCCTGAGTG GAGCAGAAAT GAAATTCCAC 
     2341 ATAGCTGTGG TGAGGTTTGT AGAAAGAAAC AGCCTGGCCA GGACTGCCCA CATTCCTGTA 
     2401 ACCTTCTCTG CCATCCAGGA CCCTGCCCAC CCTGCCCTGC CTTTATGACA AAAACATGTG 
     2461 AATGTGGACG AACCAGGCAC ACAGTTCGCT GTGGTCAGGC TGTCTCAGTC CACTGTTCTA 
     2521 ACCCATGTGA GAATATTTTG AACTGTGGTC AGCACCAGTG TGCTGAGCTG TGCCATGGGG 
     2581 GTCAGTGCCA GCCTTGCCAG ATCATTTTGA ACCAGGTATG CTATTGCGGC AGCACCTCCC 
     2641 GAGATGTGTT ATGTGGAACC GATGTAGGAA AGTCTGATGG ATTTGGGGAT TTCAGCTGTT 
     2701 TAAAGATATG TGGCAAGGAC TTGAAATGCG GTAACCATAC ATGTTCGCAA GTGTGCCACC 
     2761 CTCAGCCCTG CCAGCAATGC CCACGGCTCC CCCAGCTGGT GCGCTGTTGC CCCTGTGGCC 
     2821 AAACTCCTCT CAGCCAATTG CTAGAACTTG GAAGTAGTAG TCGGAAAACA TGCATGGACC 
     2881 CTGTGCCTTC ATGTGGAAAA GTGTGCGGCA AGCCTCTGCC TTGTGGTTCC TTAGATTTCA 
     2941 TTCATACCTG TGAAAAGCTC TGCCATGAAG GAGACTGTGG ACCATGCTCT CGCACATCAG 
     3001 TTATTTCCTG CAGATGCTCT TTCAGAACAA AGGAGCTTCC ATGTACCAGT CTCAAAAGTG 
     3061 AAGATGCTAC ATTTATGTGT GACAAGCGGT GTAACAAGAA ACGGTTGTGT GGACGGCATA 
     3121 AATGTAATGA GATATGCTGT GTGGATAAGG AGCACAAGTG TCCTTTGATT TGTGGGAGGA 
     3181 AACTCCGTTG TGGCCTTCAT AGGTGTGAAG AACCTTGTCA TCGTGGAAAC TGCCAGACAT 
     3241 GCTGGCAAGC CAGTTTTGAT GAATTAACCT GCCATTGTGG TGCATCAGTG ATTTACCCTC 
     3301 CAGTTCCCTG TGGTACTAGG CCCCCTGAAT GTACCCAAAC CTGCGCTAGA GTCCATGAGT 
     3361 GTGACCATCC AGTATATCAT TCTTGTCATA GTGAGGAGAA GTGTCCCCCT TGCACTTTCC 
     3421 TAACTCAGAA GTGGTGCATG GGCAAGCATG AGTTTCGGAG CAACATCCCC TGTCACCTGG 
     3481 TTGATATCTC TTGCGGATTA CCCTGCAGTG CCACGCTACC ATGTGGGATG CACAAATGTC 
     3541 AGAGACTCTG TCACAAAGGG GAGTGTCTTG TGGATGAGCC CTGCAAGCAG CCCTGCACCA 
     3601 CCCCCAGAGC TGACTGTGGT CACCCGTGTA TGGCACCCTG CCATACCAGC TCACCCTGCC 
     3661 CTGTGACTGC TTGTAAAGCT AAGGTAGAGC TACAGTGTGA ATGTGGACGA AGAAAAGAGA 
     3721 TGGTGATTTG CTCTGAAGCA TCTAGTACTT ATCAAAGAAT AGCTGCAATC TCCATGGCCT 
     3781 CTAAGATAAC AGACATGCAG CTTGGAGGTT CAGTGGAGAT CAGCAAGTTA ATTACCAAAA 
     3841 AGGAAGTTCA TCAAGCCAGG CTGGAGTGTG ATGAGGAGTG TTCAGCCTTG GAAAGGAAAA 
     3901 AGAGATTAGC AGAGGCATTT CATATCAGTG AGGATTCTGA TCCTTTCAAT ATACGTTCTT 
     3961 CAGGGTCAAA ATTCAGTGAT AGTTTGAAAG AAGATGCCAG GAAGGACTTA AAGTTTGTCA 
     4021 GTGACGTTGA GAAGGAAATG GAAACCCTCG TGGAGGCCGT GAATAAGGGA AAGAATAGTA 
     4081 AGAAAAGCCA CAGCTTCCCT CCCATGAACA GAGACCACCG CCGGATCATC CATGACTTGG 
     4141 CCCAAGTTTA TGGCCTGGAG AGCGTGAGCT ATGACAGTGA ACCGAAGCGC AATGTGGTGG 
     4201 TCACTGCCAT CAGGGGGAAG TCCGTTTGTC CTCCTACCAC GCTGACAGGT GTGCTTGAAA 
     4261 GGGAAATGCA GGCACGGCCT CCACCACCGA TTCCTCATCA CAGACATCAG TCAGACAAGA 
     4321 ATCCTGGGAG CAGTAATTTA CAGAAAATAA CCAAGGAGCC AATAATTGAC TATTTTGACG 
     4381 TCCAGGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4441 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4501 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4561 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4621 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4681 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4741 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4801 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4861 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4921 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4981 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5041 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5101 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5161 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5221 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5281 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5341 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5401 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5461 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5521 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5581 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5641 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5701 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5761 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5821 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5881 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5941 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6001 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6061 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6121 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6181 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6241 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6301 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6361 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6421 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6481 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6541 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6601 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6661 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6721 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6781 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6841 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6901 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6961 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7021 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7081 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7141 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7201 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7261 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7321 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7381 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7441 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7501 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7561 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7681 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7741 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7801 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7861 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7921 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7981 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8041 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8101 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8161 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8221 CAAAAATTTA ACGCGAATTT T
//